DCAF1 (DDB1 and CUL4 associated factor 1)

Gene

• Entrez ID: 9730
• Gene name: DDB1 and CUL4 associated factor 1
• Gene symbol: DCAF1
• Synonyms (NCBI Gene): RIP, VPRBP
• Chromosome: 3
• Chromosome location: 3p21.2

miRNA

miRTarBase ID	miRNA	Experiments	Reference
MIRT755336	hsa-miR-3175	Luciferase reporter assay, Western blotting, qRT-PCR, Immunoprecipitaion (IP), ELISA, RNA pull down assay	34716304

Gene ontology (GO)

GO ID	Ontology	Definition	Evidence	Reference
GO:0000122	Process	Negative regulation of transcription by RNA polymerase II	IDA	24140421
GO:0000166	Function	Nucleotide binding	IEA
GO:0001650	Component	Fibrillar center	IDA
GO:0004674	Function	Protein serine/threonine kinase activity	IEA
GO:0005515	Function	Protein binding	IPI	16949367, 17314515, 19264781, 20178741, 22157821, 22190034, 24336198, 24412650, 25026211, 32814053, 35271311
GO:0005524	Function	ATP binding	IEA
GO:0005634	Component	Nucleus	IBA
GO:0005634	Component	Nucleus	IDA	20178741
GO:0005634	Component	Nucleus	IEA
GO:0005654	Component	Nucleoplasm	IDA
GO:0005737	Component	Cytoplasm	IEA
GO:0005813	Component	Centrosome	IDA	28242748, 34259627
GO:0005813	Component	Centrosome	IEA
GO:0005856	Component	Cytoskeleton	IEA
GO:0006325	Process	Chromatin organization	IEA
GO:0006338	Process	Chromatin remodeling	IEA
GO:0008180	Component	COP9 signalosome	IDA	18850735
GO:0016301	Function	Kinase activity	IEA
GO:0016567	Process	Protein ubiquitination	IEA
GO:0016740	Function	Transferase activity	IEA
GO:0030183	Process	B cell differentiation	IEA
GO:0030183	Process	B cell differentiation	ISS
GO:0030331	Function	Nuclear estrogen receptor binding	IBA
GO:0030331	Function	Nuclear estrogen receptor binding	IPI	28068668
GO:0033151	Process	V(D)J recombination	IEA
GO:0033151	Process	V(D)J recombination	ISS
GO:0035212	Process	Cell competition in a multicellular organism	IMP	20644714
GO:0043161	Process	Proteasome-mediated ubiquitin-dependent protein catabolic process	IDA	28242748, 34259627
GO:0043687	Process	Post-translational protein modification	IDA	24140421
GO:0045732	Process	Positive regulation of protein catabolic process	IDA	11027288, 20870715
GO:0045732	Process	Positive regulation of protein catabolic process	IDA	23063525
GO:0045732	Process	Positive regulation of protein catabolic process	IMP	16949367
GO:0080008	Component	Cul4-RING E3 ubiquitin ligase complex	IBA
GO:0080008	Component	Cul4-RING E3 ubiquitin ligase complex	IDA	16949367, 28242748, 34259627
GO:0097510	Process	Base-excision repair, AP site formation via deaminated base removal	IDA	18973764
GO:0106310	Function	Protein serine kinase activity	IEA
GO:1990244	Function	Histone H2AT120 kinase activity	IBA
GO:1990244	Function	Histone H2AT120 kinase activity	IDA	24140421
GO:1990756	Function	Ubiquitin-like ligase-substrate adaptor activity	IDA	23063525, 28242748, 34259627

Other IDs

• MIM: 617259
• HGNC: 30911
• e!Ensembl: ENSG00000145041

Protein

• UniProt ID: Q9Y4B6
• Protein name: DDB1- and CUL4-associated factor 1 (HIV-1 Vpr-binding protein) (VprBP) (Serine/threonine-protein kinase VPRBP) (EC 2.7.11.1) (Vpr-interacting protein)
• Protein function: Acts both as a substrate recognition component of E3 ubiquitin-protein ligase complexes and as an atypical serine/threonine-protein kinase, playing key roles in various processes such as cell cycle, telomerase regulation and histone modification
• PDB: 3WA0, 4CC9, 4P7I, 4PXW, 4Z8L, 5AJA, 5JK7, 6N45, 6ZUE, 6ZX9, 7OKQ, 7SSE, 7UFV, 7V7B, 7V7C, 8F8E, 8OG5, 8OG6, 8OG7, 8OG8, 8OG9, 8OGA, 8OGB, 8OGC, 8OO5, 8OOD, 9B9H, 9B9T, 9B9W, 9BA2, 9BHR, 9BHS, 9C1Q, 9D4E, 9DLW
• Tissue specificity: TISSUE SPECIFICITY: Ubiquitously expressed. {ECO:0000269|PubMed:11223251}.
• Sequence:

  MTTVVVHVDSKAELTTLLEQWEKEHGSGQDMVPILTRMSQLIEKETEEYRKGDPDPFDDR
  HPGRADPECMLGHLLRILFKNDDFMNALVNAYVMTSREPPLNTAACRLLLDIMPGLETAV
  VFQEKEGIVENLFKWAREADQPLRTYSTGLLGGAMENQDIAANYRDENSQLVAIVLRRLR
  ELQLQEVALRQENKRPSPRKLSSEPLLPLDEEAVDMDYGDMAVDVVDGDQEEASGDMEIS
  FHLDSGHKTSSRVNSTTKPEDGGLKKNKSAKQGDRENFRKAKQKLGFSSSDPDRMFVELS
  NSSWSEMSPWVIGTNYTLYPMTPAIEQRLILQYLTPLGEYQELLPIFMQLGSRELMMFYI
  DLKQTNDVLLTFEALKHLASLLLHNKFATEFVAHGGVQKLLEIPRPSMAATGVSMCLYYL
  SYNQDAMERVCMHPHNVLSDVVNYTLWLMECSHASGCCHATMFFSICFSFRAVLELFDRY
  DGLRRLVNLISTLEILNLEDQGALLSDDEIFASRQTGKHTCMALRKYFEAHLAIKLEQVK
  QSLQRTEGGILVHPQPPYKACSYTHEQIVEMMEFLIEYGPAQLYWEPAEVFLKLSCVQLL
  LQLISIACNWKTYYARNDTVRFALDVLAILTVVPKIQLQLAESVDVLDEAGSTVSTVGIS
  IILGVAEGEFFIHDAEIQKSALQIIINCVCGPDNRISSIGKFISGTPRRKLPQNPKSSEH
  TLAKMWNVVQSNNGIKVLLSLLSIKMPITDADQIRALACKALVGLSRSSTVRQIISKLPL
  FSSCQIQQLMKEPVLQDKRSDHVKFCKYAAELIERVSGKPLLIGTDVSLARLQKADVVAQ
  SRISFPEKELLLLIRNHLISKGLGETATVLTKEADLPMTAASHSSAFTPVTAAASPVSLP
  RTPRIANGIATRLGSHAAVGASAPSAPTAHPQPRPPQGPLALPGPSYAGNSPLIGRISFI
  RERPSPCNGRKIRVLRQKSDHGAYSQSPAIKKQLDRHLPSPPTLDSIITEYLREQHARCK
  NPVATCPPFSLFTPHQCPEPKQRRQAPINFTSRLNRRASFPKYGGVDGGCFDRHLIFSRF
  RPISVFREANEDESGFTCCAFSARERFLMLGTCTGQLKLYNVFSGQEEASYNCHNSAITH
  LEPSRDGSLLLTSATWSQPLSALWGMKSVFDMKHSFTEDHYVEFSKHSQDRVIGTKGDIA
  HIYDIQTGNKLLTLFNPDLANNYKRNCATFNPTDDLVLNDGVLWDVRSAQAIHKFDKFNM
  NISGVFHPNGLEVIINTEIWDLRTFHLLHTVPALDQCRVVFNHTGTVMYGAMLQADDEDD
  LMEERMKSPFGSSFRTFNATDYKPIATIDVKRNIFDLCTDTKDCYLAVIENQGSMDALNM
  DTVCRLYEVGRQRLAEDEDEEEDQEEEEQEEEDDDEDDDDTDDLDELDTDQLLEAELEED
  DNNENAGEDGDNDFSPSDEELANLLEEGEDGEDEDSDADEEVELILGDTDSSDNSDLEDD
  IILSLNE

• Sequence length: 1507

Pathways

KEGG:

Human immunodeficiency virus 1 infection

Reactome:

Antigen processing: Ubiquitination & Proteasome degradation