PFKFB4 (6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4)

Gene

• Entrez ID: 5210
• Gene name: 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4
• Gene symbol: PFKFB4
• Synonyms (NCBI Gene): -
• Chromosome: 3
• Chromosome location: 3p21.31
• Summary: The protein encoded by this gene is one of four bifunctional kinase/phosphatases that regulate the concentration of the glycolytic byproduct fructose-2,6-bisphosphate (F2,6BP). The encoded protein is highly expressed in cancer cells and is induced by hypo

miRNA

miRTarBase ID	miRNA	Experiments	Reference
MIRT016861	hsa-miR-335-5p	Microarray	18185580
MIRT040714	hsa-miR-92b-3p	CLASH	23622248
MIRT1226376	hsa-miR-214	CLIP-seq
MIRT1226377	hsa-miR-23a	CLIP-seq
MIRT1226378	hsa-miR-23b	CLIP-seq
MIRT1226379	hsa-miR-23c	CLIP-seq
MIRT1226380	hsa-miR-2467-3p	CLIP-seq
MIRT1226381	hsa-miR-296-3p	CLIP-seq
MIRT1226382	hsa-miR-3124-3p	CLIP-seq
MIRT1226383	hsa-miR-3138	CLIP-seq
MIRT1226384	hsa-miR-323-5p	CLIP-seq
MIRT1226385	hsa-miR-3619-5p	CLIP-seq
MIRT1226386	hsa-miR-3687	CLIP-seq
MIRT1226387	hsa-miR-4436b-3p	CLIP-seq
MIRT1226388	hsa-miR-4442	CLIP-seq
MIRT1226389	hsa-miR-450b-3p	CLIP-seq
MIRT1226390	hsa-miR-4520a-3p	CLIP-seq
MIRT1226391	hsa-miR-4685-5p	CLIP-seq
MIRT1226392	hsa-miR-4713-3p	CLIP-seq
MIRT1226393	hsa-miR-4755-3p	CLIP-seq
MIRT1226394	hsa-miR-4786-5p	CLIP-seq
MIRT1226395	hsa-miR-591	CLIP-seq
MIRT1226396	hsa-miR-638	CLIP-seq
MIRT1226397	hsa-miR-658	CLIP-seq
MIRT1226398	hsa-miR-761	CLIP-seq
MIRT1226399	hsa-miR-769-3p	CLIP-seq
MIRT2066293	hsa-miR-1184	CLIP-seq
MIRT2066294	hsa-miR-1205	CLIP-seq
MIRT2066295	hsa-miR-1226	CLIP-seq
MIRT2066296	hsa-miR-1276	CLIP-seq
MIRT2066297	hsa-miR-15a	CLIP-seq
MIRT2066298	hsa-miR-15b	CLIP-seq
MIRT2066299	hsa-miR-16	CLIP-seq
MIRT2066300	hsa-miR-1910	CLIP-seq
MIRT2066301	hsa-miR-195	CLIP-seq
MIRT2066302	hsa-miR-2278	CLIP-seq
MIRT2066303	hsa-miR-2355-5p	CLIP-seq
MIRT2066304	hsa-miR-2861	CLIP-seq
MIRT2066305	hsa-miR-3158-5p	CLIP-seq
MIRT2066306	hsa-miR-424	CLIP-seq
MIRT1226390	hsa-miR-4520a-3p	CLIP-seq
MIRT2066307	hsa-miR-4524	CLIP-seq
MIRT2066308	hsa-miR-4642	CLIP-seq
MIRT2066309	hsa-miR-4672	CLIP-seq
MIRT1226391	hsa-miR-4685-5p	CLIP-seq
MIRT1226392	hsa-miR-4713-3p	CLIP-seq
MIRT2066310	hsa-miR-497	CLIP-seq
MIRT2066311	hsa-miR-513b	CLIP-seq
MIRT2066312	hsa-miR-518a-5p	CLIP-seq
MIRT2066313	hsa-miR-527	CLIP-seq
MIRT2066295	hsa-miR-1226	CLIP-seq
MIRT2293779	hsa-miR-1229	CLIP-seq
MIRT2293780	hsa-miR-146b-3p	CLIP-seq
MIRT2066297	hsa-miR-15a	CLIP-seq
MIRT2066298	hsa-miR-15b	CLIP-seq
MIRT2066299	hsa-miR-16	CLIP-seq
MIRT2066301	hsa-miR-195	CLIP-seq
MIRT1226376	hsa-miR-214	CLIP-seq
MIRT2066302	hsa-miR-2278	CLIP-seq
MIRT2293781	hsa-miR-3150a-3p	CLIP-seq
MIRT2293782	hsa-miR-3175	CLIP-seq
MIRT2293783	hsa-miR-3184	CLIP-seq
MIRT1226385	hsa-miR-3619-5p	CLIP-seq
MIRT2293784	hsa-miR-3663-5p	CLIP-seq
MIRT2293785	hsa-miR-423-5p	CLIP-seq
MIRT2066306	hsa-miR-424	CLIP-seq
MIRT2293786	hsa-miR-4287	CLIP-seq
MIRT2293787	hsa-miR-4313	CLIP-seq
MIRT2293788	hsa-miR-4457	CLIP-seq
MIRT2293789	hsa-miR-4469	CLIP-seq
MIRT2293790	hsa-miR-4488	CLIP-seq
MIRT1226390	hsa-miR-4520a-3p	CLIP-seq
MIRT2293791	hsa-miR-4522	CLIP-seq
MIRT2066307	hsa-miR-4524	CLIP-seq
MIRT2293792	hsa-miR-4667-5p	CLIP-seq
MIRT2293793	hsa-miR-4685-3p	CLIP-seq
MIRT2293794	hsa-miR-4697-5p	CLIP-seq
MIRT2293795	hsa-miR-4700-5p	CLIP-seq
MIRT2293796	hsa-miR-4728-5p	CLIP-seq
MIRT2293797	hsa-miR-4731-3p	CLIP-seq
MIRT2293798	hsa-miR-4733-3p	CLIP-seq
MIRT2293799	hsa-miR-4801	CLIP-seq
MIRT2066310	hsa-miR-497	CLIP-seq
MIRT2293800	hsa-miR-503	CLIP-seq
MIRT2293801	hsa-miR-622	CLIP-seq
MIRT2293802	hsa-miR-646	CLIP-seq
MIRT1226398	hsa-miR-761	CLIP-seq
MIRT2293803	hsa-miR-939	CLIP-seq
MIRT2066293	hsa-miR-1184	CLIP-seq
MIRT2591861	hsa-miR-1202	CLIP-seq
MIRT2066294	hsa-miR-1205	CLIP-seq
MIRT2066295	hsa-miR-1226	CLIP-seq
MIRT2591862	hsa-miR-197	CLIP-seq
MIRT2066302	hsa-miR-2278	CLIP-seq
MIRT1226380	hsa-miR-2467-3p	CLIP-seq
MIRT2591863	hsa-miR-30a	CLIP-seq
MIRT2591864	hsa-miR-30b	CLIP-seq
MIRT2591865	hsa-miR-30c	CLIP-seq
MIRT2591866	hsa-miR-30d	CLIP-seq
MIRT2591867	hsa-miR-30e	CLIP-seq
MIRT2066305	hsa-miR-3158-5p	CLIP-seq
MIRT2591868	hsa-miR-3194-5p	CLIP-seq
MIRT2591869	hsa-miR-3607-3p	CLIP-seq
MIRT2591870	hsa-miR-3654	CLIP-seq
MIRT2591871	hsa-miR-3678-3p	CLIP-seq
MIRT2591872	hsa-miR-378	CLIP-seq
MIRT2591873	hsa-miR-378b	CLIP-seq
MIRT2591874	hsa-miR-378c	CLIP-seq
MIRT2591875	hsa-miR-378d	CLIP-seq
MIRT2591876	hsa-miR-378e	CLIP-seq
MIRT2591877	hsa-miR-378f	CLIP-seq
MIRT2591878	hsa-miR-378h	CLIP-seq
MIRT2591879	hsa-miR-378i	CLIP-seq
MIRT2591880	hsa-miR-3922-5p	CLIP-seq
MIRT2591881	hsa-miR-3972	CLIP-seq
MIRT2591882	hsa-miR-422a	CLIP-seq
MIRT2591883	hsa-miR-4323	CLIP-seq
MIRT2293788	hsa-miR-4457	CLIP-seq
MIRT2591884	hsa-miR-4494	CLIP-seq
MIRT2591885	hsa-miR-4519	CLIP-seq
MIRT2591886	hsa-miR-4640-3p	CLIP-seq
MIRT2591887	hsa-miR-4676-5p	CLIP-seq
MIRT2293798	hsa-miR-4733-3p	CLIP-seq
MIRT2591888	hsa-miR-4753-5p	CLIP-seq
MIRT2591889	hsa-miR-4758-3p	CLIP-seq
MIRT2591890	hsa-miR-4763-5p	CLIP-seq
MIRT2591891	hsa-miR-4776-3p	CLIP-seq
MIRT2591892	hsa-miR-4798-3p	CLIP-seq
MIRT2591893	hsa-miR-5096	CLIP-seq
MIRT2066311	hsa-miR-513b	CLIP-seq
MIRT2591894	hsa-miR-515-3p	CLIP-seq
MIRT2591895	hsa-miR-519e	CLIP-seq
MIRT2591896	hsa-miR-550a	CLIP-seq
MIRT2591897	hsa-miR-575	CLIP-seq
MIRT2293801	hsa-miR-622	CLIP-seq
MIRT2066295	hsa-miR-1226	CLIP-seq
MIRT2591862	hsa-miR-197	CLIP-seq
MIRT2591868	hsa-miR-3194-5p	CLIP-seq
MIRT2687692	hsa-miR-3202	CLIP-seq
MIRT2591870	hsa-miR-3654	CLIP-seq
MIRT2591872	hsa-miR-378	CLIP-seq
MIRT2591873	hsa-miR-378b	CLIP-seq
MIRT2591874	hsa-miR-378c	CLIP-seq
MIRT2591875	hsa-miR-378d	CLIP-seq
MIRT2591876	hsa-miR-378e	CLIP-seq
MIRT2591877	hsa-miR-378f	CLIP-seq
MIRT2591878	hsa-miR-378h	CLIP-seq
MIRT2591879	hsa-miR-378i	CLIP-seq
MIRT2591880	hsa-miR-3922-5p	CLIP-seq
MIRT2591882	hsa-miR-422a	CLIP-seq
MIRT2293788	hsa-miR-4457	CLIP-seq
MIRT2687693	hsa-miR-4533	CLIP-seq
MIRT2293798	hsa-miR-4733-3p	CLIP-seq
MIRT2591888	hsa-miR-4753-5p	CLIP-seq
MIRT2687694	hsa-miR-4790-3p	CLIP-seq
MIRT2591892	hsa-miR-4798-3p	CLIP-seq
MIRT2591893	hsa-miR-5096	CLIP-seq
MIRT2066311	hsa-miR-513b	CLIP-seq

Transcription factors

Transcription factor	Regulation	Reference
HIF1A	Unknown	16311927

Gene ontology (GO)

GO ID	Ontology	Definition	Evidence	Reference
GO:0000166	Function	Nucleotide binding	IEA
GO:0003824	Function	Catalytic activity	IEA
GO:0003873	Function	6-phosphofructo-2-kinase activity	IBA
GO:0003873	Function	6-phosphofructo-2-kinase activity	IEA
GO:0003873	Function	6-phosphofructo-2-kinase activity	NAS	10095107
GO:0004331	Function	Fructose-2,6-bisphosphate 2-phosphatase activity	IBA
GO:0004331	Function	Fructose-2,6-bisphosphate 2-phosphatase activity	IEA
GO:0005515	Function	Protein binding	IPI	32296183, 33961781
GO:0005524	Function	ATP binding	IEA
GO:0005829	Component	Cytosol	IBA
GO:0005829	Component	Cytosol	TAS
GO:0006000	Process	Fructose metabolic process	IEA
GO:0006003	Process	Fructose 2,6-bisphosphate metabolic process	IBA
GO:0006003	Process	Fructose 2,6-bisphosphate metabolic process	IEA
GO:0006003	Process	Fructose 2,6-bisphosphate metabolic process	ISS
GO:0006094	Process	Gluconeogenesis	NAS	15581487
GO:0006096	Process	Glycolytic process	NAS	15581487
GO:0016301	Function	Kinase activity	IEA
GO:0016740	Function	Transferase activity	IEA
GO:0016787	Function	Hydrolase activity	IEA
GO:0043540	Component	6-phosphofructo-2-kinase/fructose-2,6-biphosphatase complex	ISS
GO:0046835	Process	Carbohydrate phosphorylation	IEA

Other IDs

• MIM: 605320
• HGNC: 8875
• e!Ensembl: ENSG00000114268

Protein

• UniProt ID: Q16877
• Protein name: 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4 (6PF-2-K/Fru-2,6-P2ase 4) (PFK/FBPase 4) (6PF-2-K/Fru-2,6-P2ase testis-type isozyme) [Includes: 6-phosphofructo-2-kinase (EC 2.7.1.105); Fructose-2,6-bisphosphatase (EC 3.1.3.46)]
• Protein function: Synthesis and degradation of fructose 2,6-bisphosphate.
• Family and domains:

  Pfam

  Accession	ID	Position in sequence	Description	Type
  PF01591	6PF2K	28 → 249	6-phosphofructo-2-kinase	Domain
  PF00300	His_Phos_1	252 → 438	Histidine phosphatase superfamily (branch 1)	Domain

• Sequence:

  MASPRELTQNPLKKIWMPYSNGRPALHACQRGVCMTNCPTLIVMVGLPARGKTYISKKLT
  RYLNWIGVPTREFNVGQYRRDVVKTYKSFEFFLPDNEEGLKIRKQCALAALRDVRRFLSE
  EGGHVAVFDATNTTRERRATIFNFGEQNGYKTFFVESICVDPEVIAANIVQVKLGSPDYV
  NRDSDEATEDFMRRIECYENSYESLDEDLDRDLSYIKIMDVGQSYVVNRVADHIQSRIVY
  YLMNIHVTPRSIYLCRHGESELNLKGRIGGDPGLSPRGREFAKSLAQFISDQNIKDLKVW
  TSQMKRTIQTAEALGVPYEQWKVLNEIDAGVCEEMTYEEIQDNYPLEFALRDQDKYRYRY
  PKGESYEDLVQRLEPVIMELERQENVLVICHQAVMRCLLAYFLDKAAEQLPYLKCPLHTV
  LKLTPVAYGCKVESIFLNVAAVNTHRDRPQNVDISRPPEEALVTVPAHQ

• Sequence length: 469

Pathways

KEGG:

Fructose and mannose metabolism
Metabolic pathways
AMPK signaling pathway

Reactome:

Regulation of glycolysis by fructose 2,6-bisphosphate metabolism