NEFM (neurofilament medium chain)

Gene

• Entrez ID: 4741
• Gene name: Neurofilament medium chain
• Gene symbol: NEFM
• Synonyms (NCBI Gene): NEF3, NF-M, NFM
• Chromosome: 8
• Chromosome location: 8p21.2
• Summary: Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons

miRNA

miRTarBase ID	miRNA	Experiments	Reference
MIRT004961	hsa-let-7a-5p	qRT-PCR	17942906
MIRT016901	hsa-miR-335-5p	Microarray	18185580
MIRT2052045	hsa-miR-586	CLIP-seq
MIRT2579604	hsa-miR-1225-3p	CLIP-seq
MIRT2579605	hsa-miR-1231	CLIP-seq
MIRT2579606	hsa-miR-3943	CLIP-seq
MIRT2579607	hsa-miR-4666-3p	CLIP-seq
MIRT2579608	hsa-miR-516b	CLIP-seq
MIRT2579609	hsa-miR-654-3p	CLIP-seq
MIRT2579604	hsa-miR-1225-3p	CLIP-seq
MIRT2579605	hsa-miR-1231	CLIP-seq
MIRT2579606	hsa-miR-3943	CLIP-seq
MIRT2579607	hsa-miR-4666-3p	CLIP-seq
MIRT2579608	hsa-miR-516b	CLIP-seq
MIRT2579609	hsa-miR-654-3p	CLIP-seq

Gene ontology (GO)

GO ID	Ontology	Definition	Evidence	Reference
GO:0005198	Function	Structural molecule activity	IEA
GO:0005200	Function	Structural constituent of cytoskeleton	IBA
GO:0005200	Function	Structural constituent of cytoskeleton	TAS	3608989
GO:0005515	Function	Protein binding	IPI	20195357, 25609649, 27173435, 28514442, 32296183, 33961781, 35271311
GO:0005737	Component	Cytoplasm	IBA
GO:0005737	Component	Cytoplasm	IEA
GO:0005856	Component	Cytoskeleton	IEA
GO:0005882	Component	Intermediate filament	IBA
GO:0005882	Component	Intermediate filament	IEA
GO:0005883	Component	Neurofilament	TAS	3608989
GO:0008017	Function	Microtubule binding	TAS	17498690
GO:0030424	Component	Axon	IBA
GO:0030424	Component	Axon	IEA
GO:0030424	Component	Axon	TAS	14662745
GO:0033693	Process	Neurofilament bundle assembly	IBA
GO:0033693	Process	Neurofilament bundle assembly	TAS	17498690
GO:0042995	Component	Cell projection	IEA
GO:0045111	Component	Intermediate filament cytoskeleton	IDA
GO:0097418	Component	Neurofibrillary tangle	IDA	21828286
GO:0099160	Component	Postsynaptic intermediate filament cytoskeleton	IBA

Other IDs

• MIM: 162250
• HGNC: 7734
• e!Ensembl: ENSG00000104722

Protein

• UniProt ID: P07197
• Protein name: Neurofilament medium polypeptide (NF-M) (160 kDa neurofilament protein) (Neurofilament 3) (Neurofilament triplet M protein)
• Protein function: Neurofilaments usually contain three intermediate filament proteins: NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form
• Family and domains:

  Pfam

  Accession	ID	Position in sequence	Description	Type
  PF04732	Filament_head	9 → 99	Intermediate filament head (DNA binding) region	Family
  PF00038	Filament	100 → 411	Intermediate filament protein	Coiled-coil

• Sequence:

  MSYTLDSLGNPSAYRRVTETRSSFSRVSGSPSSGFRSQSWSRGSPSTVSSSYKRSMLAPR
  LAYSSAMLSSAESSLDFSQSSSLLNGGSGPGGDYKLSRSNEKEQLQGLNDRFAGYIEKVH
  YLEQQNKEIEAEIQALRQKQASHAQLGDAYDQEIRELRATLEMVNHEKAQVQLDSDHLEE
  DIHRLKERFEEEARLRDDTEAAIRALRKDIEEASLVKVELDKKVQSLQDEVAFLRSNHEE
  EVADLLAQIQASHITVERKDYLKTDISTALKEIRSQLESHSDQNMHQAEEWFKCRYAKLT
  EAAEQNKEAIRSAKEEIAEYRRQLQSKSIELESVRGTKESLERQLSDIEERHNHDLSSYQ
  DTIQQLENELRGTKWEMARHLREYQDLLNVKMALDIEIAAYRKLLEGEETRFSTFAGSIT
  GPLYTHRPPITISSKIQKPKVEAPKLKVQHKFVEEIIEETKVEDEKSEMEEALTAITEEL
  AVSMKEEKKEAAEEKEEEPEAEEEEVAAKKSPVKATAPEVKEEEGEKEEEEGQEEEEEED
  EGAKSDQAEEGGSEKEGSSEKEEGEQEEGETEAEAEGEEAEAKEEKKVEEKSEEVATKEE
  LVADAKVEKPEKAKSPVPKSPVEEKGKSPVPKSPVEEKGKSPVPKSPVEEKGKSPVPKSP
  VEEKGKSPVSKSPVEEKAKSPVPKSPVEEAKSKAEVGKGEQKEEEEKEVKEAPKEEKVEK
  KEEKPKDVPEKKKAESPVKEEAVAEVVTITKSVKVHLEKETKEEGKPLQQEKEKEKAGGE
  GGSEEEGSDKGAKGSRKEDIAVNGEVEGKEEVEQETKEKGSGREEEKGVVTNGLDLSPAD
  EKKGGDKSEEKVVVTKTVEKITSEGGDGATKYITKSVTVTQKVEEHEETFEEKLVSTKKV
  EKVTSHAIVKEVTQSD

• Sequence length: 916

Pathways

KEGG:

Amyotrophic lateral sclerosis
Pathways of neurodegeneration - multiple diseases