MAP3K10 (mitogen-activated protein kinase kinase kinase 10)

Gene

• Entrez ID: 4294
• Gene name: Mitogen-activated protein kinase kinase kinase 10
• Gene symbol: MAP3K10
• Synonyms (NCBI Gene): MEKK10, MLK2, MST
• Chromosome: 19
• Chromosome location: 19q13.2
• Summary: The protein encoded by this gene is a member of the serine/threonine kinase family. This kinase has been shown to activate MAPK8/JNK and MKK4/SEK1, and this kinase itself can be phoshorylated, and thus activated by JNK kinases. This kinase functions prefe

miRNA

miRTarBase ID	miRNA	Experiments	Reference
MIRT005545	hsa-miR-181b-5p	qRT-PCR, Western blot	20596961
MIRT005545	hsa-miR-181b-5p	qRT-PCR, Western blot	20596961
MIRT007103	hsa-miR-155-5p	Luciferase reporter assay	23189122
MIRT007103	hsa-miR-155-5p	Luciferase reporter assay	23189122
MIRT007103	hsa-miR-155-5p	Reporter assay;Other	20584899
MIRT042327	hsa-miR-484	CLASH	23622248
MIRT053691	hsa-miR-181a-5p	Microarray	22942087
MIRT007103	hsa-miR-155-5p	HITS-CLIP	22473208
MIRT007103	hsa-miR-155-5p	HITS-CLIP	22473208
MIRT732536	hsa-miR-146b-3p	Microarray, qRT-PCR, Western blotting	33995647
MIRT2036441	hsa-miR-1207-5p	CLIP-seq
MIRT2036442	hsa-miR-1915	CLIP-seq
MIRT2036443	hsa-miR-361-3p	CLIP-seq
MIRT2036444	hsa-miR-3918	CLIP-seq
MIRT2036445	hsa-miR-4492	CLIP-seq
MIRT2036446	hsa-miR-4498	CLIP-seq
MIRT2036447	hsa-miR-4505	CLIP-seq
MIRT2036448	hsa-miR-4512	CLIP-seq
MIRT2036449	hsa-miR-4685-5p	CLIP-seq
MIRT2036450	hsa-miR-4722-3p	CLIP-seq
MIRT2036451	hsa-miR-4731-5p	CLIP-seq
MIRT2036452	hsa-miR-4763-3p	CLIP-seq
MIRT2036453	hsa-miR-4793-5p	CLIP-seq
MIRT2036454	hsa-miR-486-3p	CLIP-seq
MIRT2036455	hsa-miR-762	CLIP-seq
MIRT2036441	hsa-miR-1207-5p	CLIP-seq
MIRT2266701	hsa-miR-1224-3p	CLIP-seq
MIRT2036442	hsa-miR-1915	CLIP-seq
MIRT2036443	hsa-miR-361-3p	CLIP-seq
MIRT2036444	hsa-miR-3918	CLIP-seq
MIRT2036448	hsa-miR-4512	CLIP-seq
MIRT2036449	hsa-miR-4685-5p	CLIP-seq
MIRT2036450	hsa-miR-4722-3p	CLIP-seq
MIRT2036452	hsa-miR-4763-3p	CLIP-seq
MIRT2036454	hsa-miR-486-3p	CLIP-seq
MIRT2266701	hsa-miR-1224-3p	CLIP-seq
MIRT2566311	hsa-miR-1908	CLIP-seq
MIRT2566312	hsa-miR-3622b-5p	CLIP-seq
MIRT2566313	hsa-miR-3688-5p	CLIP-seq
MIRT2566314	hsa-miR-4474-3p	CLIP-seq
MIRT2036445	hsa-miR-4492	CLIP-seq
MIRT2036446	hsa-miR-4498	CLIP-seq
MIRT2036447	hsa-miR-4505	CLIP-seq
MIRT2036451	hsa-miR-4731-5p	CLIP-seq
MIRT2566315	hsa-miR-663	CLIP-seq
MIRT2036455	hsa-miR-762	CLIP-seq

Gene ontology (GO)

GO ID	Ontology	Definition	Evidence	Reference
GO:0003714	Function	Transcription corepressor activity	ISS
GO:0004672	Function	Protein kinase activity	IBA	21873635
GO:0004674	Function	Protein serine/threonine kinase activity	IDA	19801649
GO:0004706	Function	JUN kinase kinase kinase activity	IBA	21873635
GO:0004706	Function	JUN kinase kinase kinase activity	ISS
GO:0005524	Function	ATP binding	IEA
GO:0005737	Component	Cytoplasm	IDA	18455992
GO:0006915	Process	Apoptotic process	TAS	10801775
GO:0007165	Process	Signal transduction	TAS	10801775
GO:0007224	Process	Smoothened signaling pathway	IMP	18455992
GO:0007254	Process	JNK cascade	TAS	10801775
GO:0007256	Process	Activation of JNKK activity	IEA
GO:0007257	Process	Activation of JUN kinase activity	IBA	21873635
GO:0007257	Process	Activation of JUN kinase activity	ISS	8195146
GO:0018105	Process	Peptidyl-serine phosphorylation	IDA	19801649
GO:0018107	Process	Peptidyl-threonine phosphorylation	IDA	19801649
GO:0042803	Function	Protein homodimerization activity	ISS	8195146
GO:0043065	Process	Positive regulation of apoptotic process	IBA	21873635
GO:0043425	Function	BHLH transcription factor binding	IPI	19801649
GO:0043433	Process	Negative regulation of DNA-binding transcription factor activity	IMP	19801649
GO:0043507	Process	Positive regulation of JUN kinase activity	IMP	17584736
GO:0045892	Process	Negative regulation of transcription, DNA-templated	IMP	19801649
GO:0045892	Process	Negative regulation of transcription, DNA-templated	ISS
GO:0046330	Process	Positive regulation of JNK cascade	IMP	17584736
GO:0046777	Process	Protein autophosphorylation	ISS	8195146

Other IDs

• MIM: 600137
• HGNC: 6849
• e!Ensembl: ENSG00000130758

Protein

• UniProt ID: Q02779
• Protein name: Mitogen-activated protein kinase kinase kinase 10 (EC 2.7.11.25) (Mixed lineage kinase 2) (Protein kinase MST)
• Protein function: Activates the JUN N-terminal pathway.
• PDB: 2RF0
• Family and domains:

  Pfam

  Accession	ID	Position in sequence	Description	Type
  PF14604	SH3_9	23 → 77	Variant SH3 domain	Domain
  PF07714	PK_Tyr_Ser-Thr	98 → 357	Protein tyrosine and serine/threonine kinase	Domain

• Tissue specificity: TISSUE SPECIFICITY: Expressed in brain and skeletal muscle.
• Sequence:

  MEEEEGAVAKEWGTTPAGPVWTAVFDYEAAGDEELTLRRGDRVQVLSQDCAVSGDEGWWT
  GQLPSGRVGVFPSNYVAPGAPAAPAGLQLPQEIPFHELQLEEIIGVGGFGKVYRALWRGE
  EVAVKAARLDPEKDPAVTAEQVCQEARLFGALQHPNIIALRGACLNPPHLCLVMEYARGG
  ALSRVLAGRRVPPHVLVNWAVQVARGMNYLHNDAPVPIIHRDLKSINILILEAIENHNLA
  DTVLKITDFGLAREWHKTTKMSAAGTYAWMAPEVIRLSLFSKSSDVWSFGVLLWELLTGE
  VPYREIDALAVAYGVAMNKLTLPIPSTCPEPFARLLEECWDPDPHGRPDFGSILKRLEVI
  EQSALFQMPLESFHSLQEDWKLEIQHMFDDLRTKEKELRSREEELLRAAQEQRFQEEQLR
  RREQELAEREMDIVERELHLLMCQLSQEKPRVRKRKGNFKRSRLLKLREGGSHISLPSGF
  EHKITVQASPTLDKRKGSDGASPPASPSIIPRLRAIRLTPVDCGGSSSGSSSGGSGTWSR
  GGPPKKEELVGGKKKGRTWGPSSTLQKERVGGEERLKGLGEGSKQWSSSAPNLGKSPKHT
  PIAPGFASLNEMEEFAEAEDGGSSVPPSPYSTPSYLSVPLPAEPSPGARAPWEPTPSAPP
  ARWGHGARRRCDLALLGCATLLGAVGLGADVAEARAADGEEQRRWLDGLFFPRAGRFPRG
  LSPPARPHGRREDVGPGLGLAPSATLVSLSSVSDCNSTRSLLRSDSDEAAPAAPSPPPSP
  PAPTPTPSPSTNPLVDLELESFKKDPRQSLTPTHVTAACAVSRGHRRTPSDGALGQRGPP
  EPAGHGPGPRDLLDFPRLPDPQALFPARRRPPEFPGRPTTLTFAPRPRPAASRPRLDPWK
  LVSFGRTLTISPPSRPDTPESPGPPSVQPTLLDMDMEGQNQDSTVPLCGAHGSH

• Sequence length: 954

Pathways

KEGG:

MAPK signaling pathway
Huntington disease
Pathways of neurodegeneration - multiple diseases