IBSP (integrin binding sialoprotein)

Gene

• Entrez ID: 3381
• Gene name: Integrin binding sialoprotein
• Gene symbol: IBSP
• Synonyms (NCBI Gene): BNSP, BSP, BSP-II, SP-II
• Chromosome: 4
• Chromosome location: 4q22.1
• Summary: The protein encoded by this gene is a major structural protein of the bone matrix. It constitutes approximately 12% of the noncollagenous proteins in human bone and is synthesized by skeletal-associated cell types, including hypertrophic chondrocytes, ost

miRNA

miRTarBase ID	miRNA	Experiments	Reference
MIRT003594	hsa-miR-135b-5p	qRT-PCR	19795981
MIRT029948	hsa-miR-26b-5p	Microarray	19088304
MIRT733795	hsa-miR-92a-3p	Luciferase reporter assay, Western blotting	33717654
MIRT1058276	hsa-miR-1185	CLIP-seq
MIRT1058277	hsa-miR-128	CLIP-seq
MIRT1058278	hsa-miR-1299	CLIP-seq
MIRT1058279	hsa-miR-153	CLIP-seq
MIRT1058280	hsa-miR-196a	CLIP-seq
MIRT1058281	hsa-miR-196b	CLIP-seq
MIRT1058282	hsa-miR-3605-5p	CLIP-seq
MIRT1058283	hsa-miR-3653	CLIP-seq
MIRT1058284	hsa-miR-3658	CLIP-seq
MIRT1058285	hsa-miR-3679-5p	CLIP-seq
MIRT1058286	hsa-miR-3682-3p	CLIP-seq
MIRT1058287	hsa-miR-374c	CLIP-seq
MIRT1058288	hsa-miR-3927	CLIP-seq
MIRT1058289	hsa-miR-4275	CLIP-seq
MIRT1058290	hsa-miR-448	CLIP-seq
MIRT1058291	hsa-miR-4643	CLIP-seq
MIRT1058292	hsa-miR-466	CLIP-seq
MIRT1058293	hsa-miR-4672	CLIP-seq
MIRT1058294	hsa-miR-4684-3p	CLIP-seq
MIRT1058295	hsa-miR-4729	CLIP-seq
MIRT1058296	hsa-miR-4789-3p	CLIP-seq
MIRT1058297	hsa-miR-548c-3p	CLIP-seq
MIRT1058298	hsa-miR-655	CLIP-seq
MIRT1058299	hsa-miR-875-3p	CLIP-seq
MIRT1920495	hsa-miR-1255a	CLIP-seq
MIRT1920496	hsa-miR-1255b	CLIP-seq
MIRT1920497	hsa-miR-1915	CLIP-seq
MIRT1920498	hsa-miR-3135b	CLIP-seq
MIRT1920499	hsa-miR-3652	CLIP-seq
MIRT1920500	hsa-miR-4430	CLIP-seq
MIRT1920501	hsa-miR-4452	CLIP-seq
MIRT1920502	hsa-miR-4633-3p	CLIP-seq
MIRT1920503	hsa-miR-4726-3p	CLIP-seq
MIRT1920504	hsa-miR-4760-3p	CLIP-seq
MIRT2245652	hsa-miR-616	CLIP-seq

Transcription factors

Transcription factor	Regulation	Reference
CREB1	Unknown	23485603
DLX5	Activation	16000302
DLX5	Unknown	23485603
FOSL2	Unknown	23485603
JUN	Unknown	23485603;9084640
MSX2	Unknown	23485603
RUNX2	Activation	16000302
RUNX2	Repression	17956871
RUNX2	Unknown	23485603
SMAD1	Unknown	23485603

Gene ontology (GO)

GO ID	Ontology	Definition	Evidence	Reference
GO:0001503	Process	Ossification	IEA
GO:0001649	Process	Osteoblast differentiation	HDA	16210410
GO:0005178	Function	Integrin binding	IMP	24103036
GO:0005515	Function	Protein binding	IPI	10747989, 32296183
GO:0005576	Component	Extracellular region	IEA
GO:0005576	Component	Extracellular region	TAS
GO:0005615	Component	Extracellular space	ISS
GO:0007155	Process	Cell adhesion	IEA
GO:0007155	Process	Cell adhesion	ISS
GO:0016020	Component	Membrane	HDA	16210410
GO:0030198	Process	Extracellular matrix organization	IBA
GO:0030198	Process	Extracellular matrix organization	IEA
GO:0030198	Process	Extracellular matrix organization	ISS
GO:0030282	Process	Bone mineralization	IBA
GO:0030282	Process	Bone mineralization	IEA
GO:0031214	Process	Biomineral tissue development	IEA
GO:0031982	Component	Vesicle	ISS
GO:0036094	Function	Small molecule binding	IPI	9258751
GO:0045785	Process	Positive regulation of cell adhesion	IMP	24103036
GO:0071363	Process	Cellular response to growth factor stimulus	IEA
GO:0071363	Process	Cellular response to growth factor stimulus	ISS

Other IDs

• MIM: 147563
• HGNC: 5341
• e!Ensembl: ENSG00000029559

Protein

• UniProt ID: P21815
• Protein name: Integrin-binding sialoprotein (Bone sialoprotein 2) (Bone sialoprotein II) (BSP II) (Cell-binding sialoprotein)
• Protein function: Binds tightly to hydroxyapatite (PubMed:11459848). Appears to form an integral part of the mineralized matrix (PubMed:1818768). Probably important to cell-matrix interaction (PubMed:1818768). Promotes adhesion and migration of various cells via
• Family and domains:

  Pfam

  Accession	ID	Position in sequence	Description	Type
  PF05432	BSP_II	17 → 314	Bone sialoprotein II (BSP-II)	Family

• Tissue specificity: TISSUE SPECIFICITY: Expressed in bone (at protein level) (PubMed:11459848). Expressed in trophoblast cells of placenta (at protein level) (PubMed:1818768). Expressed in brain (PubMed:36261010). {ECO:0000269|PubMed:11459848, ECO:0000269|PubMed:1818768, ECO
• Sequence:

  MKTALILLSILGMACAFSMKNLHRRVKIEDSEENGVFKYRPRYYLYKHAYFYPHLKRFPV
  QGSSDSSEENGDDSSEEEEEEEETSNEGENNEESNEDEDSEAENTTLSATTLGYGEDATP
  GTGYTGLAAIQLPKKAGDITNKATKEKESDEEEEEEEEGNENEESEAEVDENEQGINGTS
  TNSTEAENGNGSSGGDNGEEGEEESVTGANAEDTTETGRQGKGTSKTTTSPNGGFEPTTP
  PQVYRTTSPPFGKTTTVEYEGEYEYTGANEYDNGYEIYESENGEPRGDNYRAYEDEYSYF
  KGQGYDGYDGQNYYHHQ

• Sequence length: 317

Pathways

KEGG:

PI3K-Akt signaling pathway
Focal adhesion
ECM-receptor interaction
Human papillomavirus infection

Reactome:

Integrin cell surface interactions