MCF2L (MCF.2 cell line derived transforming sequence like)

Gene

• Entrez ID: 23263
• Gene name: MCF.2 cell line derived transforming sequence like
• Gene symbol: MCF2L
• Synonyms (NCBI Gene): ARHGEF14, DBS, OST
• Chromosome: 13
• Chromosome location: 13q34
• Summary: This gene encodes a guanine nucleotide exchange factor that interacts specifically with the GTP-bound Rac1 and plays a role in the Rho/Rac signaling pathways. A variant in this gene was associated with osteoarthritis. Alternative splicing results in multi

miRNA

miRTarBase ID	miRNA	Experiments	Reference
MIRT736677	hsa-miR-140-3p	Luciferase reporter assay, Western blotting, qRT-PCR	31732956
MIRT1136833	hsa-miR-128	CLIP-seq
MIRT1136834	hsa-miR-148a	CLIP-seq
MIRT1136835	hsa-miR-148b	CLIP-seq
MIRT1136836	hsa-miR-152	CLIP-seq
MIRT1136837	hsa-miR-19a	CLIP-seq
MIRT1136838	hsa-miR-19b	CLIP-seq
MIRT1136839	hsa-miR-4697-3p	CLIP-seq
MIRT1136840	hsa-miR-4701-5p	CLIP-seq
MIRT1136841	hsa-miR-588	CLIP-seq
MIRT1136842	hsa-miR-640	CLIP-seq
MIRT1136843	hsa-miR-134	CLIP-seq
MIRT1136844	hsa-miR-145	CLIP-seq
MIRT1136845	hsa-miR-1913	CLIP-seq
MIRT1136846	hsa-miR-198	CLIP-seq
MIRT1136847	hsa-miR-2278	CLIP-seq
MIRT1136848	hsa-miR-3118	CLIP-seq
MIRT1136849	hsa-miR-3160-3p	CLIP-seq
MIRT1136850	hsa-miR-3164	CLIP-seq
MIRT1136851	hsa-miR-3168	CLIP-seq
MIRT1136852	hsa-miR-3180-5p	CLIP-seq
MIRT1136853	hsa-miR-3200-5p	CLIP-seq
MIRT1136854	hsa-miR-324-3p	CLIP-seq
MIRT1136855	hsa-miR-4277	CLIP-seq
MIRT1136856	hsa-miR-4303	CLIP-seq
MIRT1136857	hsa-miR-4468	CLIP-seq
MIRT1136858	hsa-miR-4487	CLIP-seq
MIRT1136859	hsa-miR-4494	CLIP-seq
MIRT1136840	hsa-miR-4701-5p	CLIP-seq
MIRT1136860	hsa-miR-4771	CLIP-seq
MIRT1136861	hsa-miR-4777-5p	CLIP-seq
MIRT1136862	hsa-miR-518d-5p	CLIP-seq
MIRT1136863	hsa-miR-519b-5p	CLIP-seq
MIRT1136864	hsa-miR-519c-5p	CLIP-seq
MIRT1136865	hsa-miR-520c-5p	CLIP-seq
MIRT1136866	hsa-miR-526a	CLIP-seq
MIRT1136867	hsa-miR-558	CLIP-seq
MIRT1136841	hsa-miR-588	CLIP-seq
MIRT1136842	hsa-miR-640	CLIP-seq
MIRT2038910	hsa-miR-3941	CLIP-seq
MIRT2038911	hsa-miR-4462	CLIP-seq
MIRT2038912	hsa-miR-4762-3p	CLIP-seq
MIRT2268884	hsa-miR-30a	CLIP-seq
MIRT2268885	hsa-miR-30b	CLIP-seq
MIRT2268886	hsa-miR-30c	CLIP-seq
MIRT2268887	hsa-miR-30d	CLIP-seq
MIRT2268888	hsa-miR-30e	CLIP-seq
MIRT2268889	hsa-miR-3609	CLIP-seq
MIRT2268890	hsa-miR-4495	CLIP-seq
MIRT2268891	hsa-miR-548ah	CLIP-seq
MIRT2268892	hsa-miR-101	CLIP-seq
MIRT2268893	hsa-miR-106a	CLIP-seq
MIRT2268894	hsa-miR-106b	CLIP-seq
MIRT2268895	hsa-miR-1236	CLIP-seq
MIRT2268896	hsa-miR-1255a	CLIP-seq
MIRT2268897	hsa-miR-1255b	CLIP-seq
MIRT2268898	hsa-miR-127-5p	CLIP-seq
MIRT1136833	hsa-miR-128	CLIP-seq
MIRT2268899	hsa-miR-1285	CLIP-seq
MIRT2268900	hsa-miR-1304	CLIP-seq
MIRT2268901	hsa-miR-1343	CLIP-seq
MIRT2268902	hsa-miR-144	CLIP-seq
MIRT2268903	hsa-miR-1468	CLIP-seq
MIRT2268904	hsa-miR-1587	CLIP-seq
MIRT2268905	hsa-miR-15a	CLIP-seq
MIRT2268906	hsa-miR-15b	CLIP-seq
MIRT2268907	hsa-miR-16	CLIP-seq
MIRT2268908	hsa-miR-17	CLIP-seq
MIRT2268909	hsa-miR-195	CLIP-seq
MIRT2268910	hsa-miR-20a	CLIP-seq
MIRT2268911	hsa-miR-20b	CLIP-seq
MIRT2268912	hsa-miR-2115	CLIP-seq
MIRT2268913	hsa-miR-214	CLIP-seq
MIRT2268914	hsa-miR-217	CLIP-seq
MIRT2268915	hsa-miR-22	CLIP-seq
MIRT2268916	hsa-miR-2964a-5p	CLIP-seq
MIRT2268917	hsa-miR-298	CLIP-seq
MIRT2268884	hsa-miR-30a	CLIP-seq
MIRT2268885	hsa-miR-30b	CLIP-seq
MIRT2268886	hsa-miR-30c	CLIP-seq
MIRT2268887	hsa-miR-30d	CLIP-seq
MIRT2268888	hsa-miR-30e	CLIP-seq
MIRT2268918	hsa-miR-3115	CLIP-seq
MIRT2268919	hsa-miR-3125	CLIP-seq
MIRT2268920	hsa-miR-3127-5p	CLIP-seq
MIRT2268921	hsa-miR-3133	CLIP-seq
MIRT2268922	hsa-miR-3140-3p	CLIP-seq
MIRT2268923	hsa-miR-3145-3p	CLIP-seq
MIRT2268924	hsa-miR-3150a-3p	CLIP-seq
MIRT2268925	hsa-miR-3158-5p	CLIP-seq
MIRT2268926	hsa-miR-3165	CLIP-seq
MIRT2268927	hsa-miR-3175	CLIP-seq
MIRT2268928	hsa-miR-3176	CLIP-seq
MIRT2268929	hsa-miR-3184	CLIP-seq
MIRT2268930	hsa-miR-3185	CLIP-seq
MIRT2268931	hsa-miR-3187-5p	CLIP-seq
MIRT2268932	hsa-miR-3192	CLIP-seq
MIRT2268933	hsa-miR-320a	CLIP-seq
MIRT2268934	hsa-miR-320b	CLIP-seq
MIRT2268935	hsa-miR-320c	CLIP-seq
MIRT2268936	hsa-miR-320d	CLIP-seq
MIRT2268937	hsa-miR-323-5p	CLIP-seq
MIRT2268938	hsa-miR-329	CLIP-seq
MIRT2268939	hsa-miR-337-3p	CLIP-seq
MIRT2268940	hsa-miR-3607-3p	CLIP-seq
MIRT2268889	hsa-miR-3609	CLIP-seq
MIRT2268941	hsa-miR-3613-3p	CLIP-seq
MIRT2268942	hsa-miR-3619-5p	CLIP-seq
MIRT2268943	hsa-miR-3620	CLIP-seq
MIRT2268944	hsa-miR-3622a-3p	CLIP-seq
MIRT2268945	hsa-miR-3622b-3p	CLIP-seq
MIRT2268946	hsa-miR-362-3p	CLIP-seq
MIRT2268947	hsa-miR-3671	CLIP-seq
MIRT2268948	hsa-miR-3675-5p	CLIP-seq
MIRT2268949	hsa-miR-3681	CLIP-seq
MIRT2268950	hsa-miR-3686	CLIP-seq
MIRT2268951	hsa-miR-3689a-3p	CLIP-seq
MIRT2268952	hsa-miR-3689c	CLIP-seq
MIRT2268953	hsa-miR-3689d	CLIP-seq
MIRT2268954	hsa-miR-3691-5p	CLIP-seq
MIRT2268955	hsa-miR-378g	CLIP-seq
MIRT2268956	hsa-miR-3916	CLIP-seq
MIRT2268957	hsa-miR-3922-3p	CLIP-seq
MIRT2268958	hsa-miR-3926	CLIP-seq
MIRT2038910	hsa-miR-3941	CLIP-seq
MIRT2268959	hsa-miR-3973	CLIP-seq
MIRT2268960	hsa-miR-423-5p	CLIP-seq
MIRT2268961	hsa-miR-424	CLIP-seq
MIRT2268962	hsa-miR-4254	CLIP-seq
MIRT2268963	hsa-miR-4257	CLIP-seq
MIRT2268964	hsa-miR-4270	CLIP-seq
MIRT2268965	hsa-miR-4272	CLIP-seq
MIRT2268966	hsa-miR-4282	CLIP-seq
MIRT2268967	hsa-miR-4291	CLIP-seq
MIRT2268968	hsa-miR-4299	CLIP-seq
MIRT2268969	hsa-miR-4300	CLIP-seq
MIRT2268970	hsa-miR-4308	CLIP-seq
MIRT2268971	hsa-miR-4429	CLIP-seq
MIRT2268972	hsa-miR-4435	CLIP-seq
MIRT2268973	hsa-miR-4441	CLIP-seq
MIRT2268974	hsa-miR-4486	CLIP-seq
MIRT2268975	hsa-miR-4492	CLIP-seq
MIRT2268890	hsa-miR-4495	CLIP-seq
MIRT2268976	hsa-miR-4498	CLIP-seq
MIRT2268977	hsa-miR-4511	CLIP-seq
MIRT2268978	hsa-miR-4643	CLIP-seq
MIRT2268979	hsa-miR-4645-3p	CLIP-seq
MIRT2268980	hsa-miR-4656	CLIP-seq
MIRT2268981	hsa-miR-466	CLIP-seq
MIRT2268982	hsa-miR-4666-5p	CLIP-seq
MIRT2268983	hsa-miR-4672	CLIP-seq
MIRT2268984	hsa-miR-4675	CLIP-seq
MIRT2268985	hsa-miR-4680-5p	CLIP-seq
MIRT2268986	hsa-miR-4691-3p	CLIP-seq
MIRT1136840	hsa-miR-4701-5p	CLIP-seq
MIRT2268987	hsa-miR-4717-3p	CLIP-seq
MIRT2268988	hsa-miR-4728-5p	CLIP-seq
MIRT2268989	hsa-miR-4738-3p	CLIP-seq
MIRT2268990	hsa-miR-4741	CLIP-seq
MIRT2268991	hsa-miR-4763-5p	CLIP-seq
MIRT2268992	hsa-miR-4789-3p	CLIP-seq
MIRT2268993	hsa-miR-4796-3p	CLIP-seq
MIRT2268994	hsa-miR-494	CLIP-seq
MIRT2268995	hsa-miR-497	CLIP-seq
MIRT2268996	hsa-miR-499-3p	CLIP-seq
MIRT2268997	hsa-miR-499a-3p	CLIP-seq
MIRT2268998	hsa-miR-515-3p	CLIP-seq
MIRT2268999	hsa-miR-516a-3p	CLIP-seq
MIRT2269000	hsa-miR-519d	CLIP-seq
MIRT2269001	hsa-miR-519e	CLIP-seq
MIRT2269002	hsa-miR-542-3p	CLIP-seq
MIRT2268891	hsa-miR-548ah	CLIP-seq
MIRT2269003	hsa-miR-548k	CLIP-seq
MIRT2269004	hsa-miR-548t	CLIP-seq
MIRT2269005	hsa-miR-587	CLIP-seq
MIRT1136841	hsa-miR-588	CLIP-seq
MIRT2269006	hsa-miR-593	CLIP-seq
MIRT2269007	hsa-miR-598	CLIP-seq
MIRT2269008	hsa-miR-603	CLIP-seq
MIRT2269009	hsa-miR-612	CLIP-seq
MIRT2269010	hsa-miR-616	CLIP-seq
MIRT2269011	hsa-miR-626	CLIP-seq
MIRT2269012	hsa-miR-642b	CLIP-seq
MIRT2269013	hsa-miR-761	CLIP-seq
MIRT2269014	hsa-miR-762	CLIP-seq
MIRT2269015	hsa-miR-876-3p	CLIP-seq
MIRT2269016	hsa-miR-920	CLIP-seq
MIRT2269017	hsa-miR-922	CLIP-seq
MIRT2269018	hsa-miR-93	CLIP-seq
MIRT2269019	hsa-miR-939	CLIP-seq
MIRT2390699	hsa-miR-1207-5p	CLIP-seq
MIRT2390700	hsa-miR-3714	CLIP-seq
MIRT2268965	hsa-miR-4272	CLIP-seq
MIRT2390701	hsa-miR-4532	CLIP-seq
MIRT2390702	hsa-miR-4632	CLIP-seq
MIRT2390703	hsa-miR-4666-3p	CLIP-seq
MIRT2390704	hsa-miR-4736	CLIP-seq
MIRT2390705	hsa-miR-4763-3p	CLIP-seq
MIRT2268991	hsa-miR-4763-5p	CLIP-seq
MIRT2390706	hsa-miR-548an	CLIP-seq
MIRT2390707	hsa-miR-566	CLIP-seq
MIRT2269007	hsa-miR-598	CLIP-seq
MIRT2453823	hsa-miR-1287	CLIP-seq
MIRT2453824	hsa-miR-3135b	CLIP-seq
MIRT2453825	hsa-miR-3148	CLIP-seq
MIRT2453826	hsa-miR-3912	CLIP-seq
MIRT2453827	hsa-miR-875-3p	CLIP-seq
MIRT2268915	hsa-miR-22	CLIP-seq
MIRT2268919	hsa-miR-3125	CLIP-seq
MIRT2268933	hsa-miR-320a	CLIP-seq
MIRT2268934	hsa-miR-320b	CLIP-seq
MIRT2268935	hsa-miR-320c	CLIP-seq
MIRT2268936	hsa-miR-320d	CLIP-seq
MIRT2268956	hsa-miR-3916	CLIP-seq
MIRT2268963	hsa-miR-4257	CLIP-seq
MIRT2268971	hsa-miR-4429	CLIP-seq
MIRT2268915	hsa-miR-22	CLIP-seq
MIRT2268919	hsa-miR-3125	CLIP-seq
MIRT2268933	hsa-miR-320a	CLIP-seq
MIRT2268934	hsa-miR-320b	CLIP-seq
MIRT2268935	hsa-miR-320c	CLIP-seq
MIRT2268936	hsa-miR-320d	CLIP-seq
MIRT2268956	hsa-miR-3916	CLIP-seq
MIRT2268963	hsa-miR-4257	CLIP-seq
MIRT2268971	hsa-miR-4429	CLIP-seq

Gene ontology (GO)

GO ID	Ontology	Definition	Evidence	Reference
GO:0005085	Function	Guanyl-nucleotide exchange factor activity	IBA
GO:0005085	Function	Guanyl-nucleotide exchange factor activity	IDA	15157669
GO:0005085	Function	Guanyl-nucleotide exchange factor activity	IEA
GO:0005085	Function	Guanyl-nucleotide exchange factor activity	TAS
GO:0005515	Function	Protein binding	IPI	32814053
GO:0005615	Component	Extracellular space	HDA	22664934
GO:0005737	Component	Cytoplasm	IBA
GO:0005737	Component	Cytoplasm	IDA	15157669
GO:0005737	Component	Cytoplasm	IEA
GO:0005829	Component	Cytosol	IEA
GO:0005829	Component	Cytosol	TAS
GO:0005886	Component	Plasma membrane	IEA
GO:0008289	Function	Lipid binding	IEA
GO:0012505	Component	Endomembrane system	IDA	15157669
GO:0012505	Component	Endomembrane system	IEA
GO:0016020	Component	Membrane	IEA
GO:0031234	Component	Extrinsic component of cytoplasmic side of plasma membrane	IDA	15157669
GO:0035025	Process	Positive regulation of Rho protein signal transduction	IDA	15157669
GO:0035091	Function	Phosphatidylinositol binding	IDA	15157669
GO:0035091	Function	Phosphatidylinositol binding	IEA
GO:0035556	Process	Intracellular signal transduction	IEA
GO:0051056	Process	Regulation of small GTPase mediated signal transduction	IEA
GO:0051056	Process	Regulation of small GTPase mediated signal transduction	TAS

Other IDs

• MIM: 609499
• HGNC: 14576
• e!Ensembl: ENSG00000126217

Protein

• UniProt ID: O15068
• Protein name: Guanine nucleotide exchange factor DBS (DBL's big sister) (MCF2-transforming sequence-like protein)
• Protein function: Guanine nucleotide exchange factor that catalyzes guanine nucleotide exchange on RHOA and CDC42, and thereby contributes to the regulation of RHOA and CDC42 signaling pathways (By similarity). Seems to lack activity with RAC1. Becomes activated
• Family and domains:

  Pfam

  Accession	ID	Position in sequence	Description	Type
  PF13716	CRAL_TRIO_2	91 → 225	Divergent CRAL/TRIO domain	Domain
  PF00435	Spectrin	351 → 456	Spectrin repeat	Domain
  PF00621	RhoGEF	635 → 809	RhoGEF domain	Domain
  PF00169	PH	830 → 945	PH domain	Domain
  PF07653	SH3_2	1059 → 1114	Variant SH3 domain	Domain

• Sequence:

  MFDCWRFILCKRPGSNSYSSPQRPNEAKKEETDHQIDVSDVIRLVQDTPEATAMATDEIM
  HQDIVPLCAADIQDQLKKRFAYLSGGRGQDGSPVITFPDYPAFSEIPDKEFQNVMTYLTS
  IPSLQDAGIGFILVIDRRRDKWTSVKASVLRIAASFPANLQLVLVLRPTGFFQRTLSDIA
  FKFNRDDFKMKVPVIMLSSVPDLHGYIDKSQLTEDLGGTLDYCHSRWLCQRTAIESFALM
  VKQTAQMLQSFGTELAETELPNDVQSTSSVLCAHTEKKDKAKEDLRLALKEGHSVLESLR
  ELQAEGSEPSVNQDQLDNQATVQRLLAQLNETEAAFDEFWAKHQQKLEQCLQLRHFEQGF
  REVKAILDAASQKIATFTDIGNSLAHVEHLLRDLASFEEKSGVAVERARALSLDGEQLIG
  NKHYAVDSIRPKCQELRHLCDQFSAEIARRRGLLSKSLELHRRLETSMKWCDEGIYLLAS
  QPVDKCQSQDGAEAALQEIEKFLETGAENKIQELNAIYKEYESILNQDLMEHVRKVFQKQ
  ASMEEVFHRRQASLKKLAARQTRPVQPVAPRPEALAKSPCPSPGIRRGSENSSSEGGALR
  RGPYRRAKSEMSESRQGRGSAGEEEESLAILRRHVMSELLDTERAYVEELLCVLEGYAAE
  MDNPLMAHLLSTGLHNKKDVLFGNMEEIYHFHNRIFLRELENYTDCPELVGRCFLERMED
  FQIYEKYCQNKPRSESLWRQCSDCPFFQECQRKLDHKLSLDSYLLKPVQRITKYQLLLKE
  MLKYSRNCEGAEDLQEALSSILGILKAVNDSMHLIAITGYDGNLGDLGKLLMQGSFSVWT
  DHKRGHTKVKELARFKPMQRHLFLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMAAVG
  ITENVKGDAKKFEIWYNAREEVYIVQAPTPEIKAAWVNEIRKVLTSQLQACREASQHRAL
  EQSQSLPLPAPTSTSPSRGNSRNIKKLEERKTDPLSLEGYVSSAPLTKPPEKGKGWSKTS
  HSLEAPEDDGGWSSAEEQINSSDAEEDGGLGPKKLVPGKYTVVADHEKGGPDALRVRSGD
  VVELVQEGDEGLWYVRDPTTGKEGWVPASSLSVRLGPSGSAQCLSSSGKAHVPRAHP

• Sequence length: 1137

Pathways

Reactome:

NRAGE signals death through JNK
Rho GTPase cycle
G alpha (12/13) signalling events

Associated diseases

Unknown
Phenotype Name	Clinical Significance	dbSNP ID	RCV Accession
Cervical cancer	Benign	rs186373570	RCV005907727
Familial cancer of breast	Benign	rs186373570	RCV005907726

Associations from Text Mining
Disease Name	Relationship Type	References
Aging Premature	Associate	37110517
Alzheimer Disease	Associate	30045751, 33069246
Atherosclerosis	Associate	25898923
Breast Neoplasms	Associate	19366686, 25483302, 37110517
Cardiovascular Diseases	Associate	25898923
Myocardial Infarction	Associate	39748436
Neoplasms	Associate	23555185, 25483302
Osteoarthritis	Associate	25483302, 26584642
Osteoarthritis Knee	Associate	22615457
Prostatic Neoplasms	Associate	23555185