NID2 (nidogen 2)

Gene

• Entrez ID: 22795
• Gene name: Nidogen 2
• Gene symbol: NID2
• Synonyms (NCBI Gene): NID-2
• Chromosome: 14
• Chromosome location: 14q22.1
• Summary: This gene encodes a member of the nidogen family of basement membrane proteins. This protein is a cell-adhesion protein that binds collagens I and IV and laminin and may be involved in maintaining the structure of the basement membrane.[provided by RefSeq

miRNA

miRTarBase ID	miRNA	Experiments	Reference
MIRT022756	hsa-miR-124-3p	Microarray	18668037
MIRT052404	hsa-let-7a-5p	CLASH	23622248
MIRT041174	hsa-miR-181d-5p	CLASH	23622248
MIRT1184851	hsa-miR-33a	CLIP-seq
MIRT1184852	hsa-miR-33b	CLIP-seq
MIRT1184853	hsa-miR-4277	CLIP-seq
MIRT1184854	hsa-miR-544	CLIP-seq
MIRT2053603	hsa-miR-1182	CLIP-seq
MIRT2053604	hsa-miR-1208	CLIP-seq
MIRT2053605	hsa-miR-1264	CLIP-seq
MIRT2053606	hsa-miR-2116	CLIP-seq
MIRT2053607	hsa-miR-2909	CLIP-seq
MIRT2053608	hsa-miR-4677-5p	CLIP-seq
MIRT2431604	hsa-miR-2467-3p	CLIP-seq
MIRT2431605	hsa-miR-3678-3p	CLIP-seq
MIRT2431606	hsa-miR-4257	CLIP-seq
MIRT2431607	hsa-miR-548c-3p	CLIP-seq
MIRT2455177	hsa-miR-1252	CLIP-seq
MIRT2455178	hsa-miR-218	CLIP-seq
MIRT2455179	hsa-miR-3159	CLIP-seq
MIRT2455180	hsa-miR-3187-3p	CLIP-seq
MIRT2455181	hsa-miR-4435	CLIP-seq
MIRT2455182	hsa-miR-4470	CLIP-seq
MIRT2455183	hsa-miR-4701-5p	CLIP-seq
MIRT2455184	hsa-miR-4717-3p	CLIP-seq
MIRT2455185	hsa-miR-4761-5p	CLIP-seq
MIRT2455186	hsa-miR-4775	CLIP-seq
MIRT2455187	hsa-miR-548s	CLIP-seq
MIRT2455188	hsa-miR-578	CLIP-seq
MIRT2455189	hsa-miR-588	CLIP-seq
MIRT2455190	hsa-miR-636	CLIP-seq
MIRT2455191	hsa-miR-651	CLIP-seq

Gene ontology (GO)

GO ID	Ontology	Definition	Evidence	Reference
GO:0005201	Function	Extracellular matrix structural constituent	ISS
GO:0005201	Function	Extracellular matrix structural constituent	RCA	20551380, 27068509, 27559042, 28327460, 28675934
GO:0005509	Function	Calcium ion binding	IEA
GO:0005515	Function	Protein binding	IPI	11956183
GO:0005518	Function	Collagen binding	IEA
GO:0005518	Function	Collagen binding	TAS	9733643
GO:0005576	Component	Extracellular region	IEA
GO:0005576	Component	Extracellular region	TAS
GO:0005604	Component	Basement membrane	IBA
GO:0005604	Component	Basement membrane	IDA	22952693
GO:0005604	Component	Basement membrane	IEA
GO:0005604	Component	Basement membrane	TAS	9733643
GO:0005615	Component	Extracellular space	IBA
GO:0005886	Component	Plasma membrane	IDA
GO:0007155	Process	Cell adhesion	IEA
GO:0007155	Process	Cell adhesion	TAS	9733643
GO:0007160	Process	Cell-matrix adhesion	IBA
GO:0007160	Process	Cell-matrix adhesion	IEA
GO:0007160	Process	Cell-matrix adhesion	IEA
GO:0031012	Component	Extracellular matrix	HDA	20551380
GO:0031012	Component	Extracellular matrix	HDA	23658023, 27068509, 27559042, 28327460, 28675934
GO:0031012	Component	Extracellular matrix	IEA
GO:0070062	Component	Extracellular exosome	HDA	23533145
GO:0071711	Process	Basement membrane organization	IEA
GO:0071711	Process	Basement membrane organization	TAS	22952693

Other IDs

• MIM: 605399
• HGNC: 13389
• e!Ensembl: ENSG00000087303

Protein

• UniProt ID: Q14112
• Protein name: Nidogen-2 (NID-2) (Osteonidogen)
• Protein function: Cell adhesion glycoprotein which is widely distributed in basement membranes. Binds to collagens I and IV, to perlecan and to laminin 1. Does not bind fibulins. It probably has a role in cell-extracellular matrix interactions.
• Family and domains:

  Pfam

  Accession	ID	Position in sequence	Description	Type
  PF06119	NIDO	179 → 272	Nidogen-like	Family
  PF07474	G2F	527 → 713	G2F domain	Domain
  PF12947	EGF_3	896 → 929	EGF domain	Domain
  PF00086	Thyroglobulin_1	940 → 1005	Thyroglobulin type-1 repeat	Domain
  PF00086	Thyroglobulin_1	1019 → 1084	Thyroglobulin type-1 repeat	Domain
  PF00058	Ldl_recept_b	1198 → 1238	Low-density lipoprotein receptor repeat class B	Repeat
  PF00058	Ldl_recept_b	1241 → 1283	Low-density lipoprotein receptor repeat class B	Repeat

• Tissue specificity: TISSUE SPECIFICITY: Detected in placenta (at protein level) (PubMed:32337544). Heart and bone. Less in pancreas, kidney and skeletal muscle. {ECO:0000269|PubMed:32337544}.
• Sequence:

  MEGDRVAGRPVLSSLPVLLLLPLLMLRAAALHPDELFPHGESWGDQLLQEGDDESSAVVK
  LANPLHFYEARFSNLYVGTNGIISTQDFPRETQYVDYDFPTDFPAIAPFLADIDTSHGRG
  RVLYREDTSPAVLGLAARYVRAGFPRSARFTPTHAFLATWEQVGAYEEVKRGALPSGELN
  TFQAVLASDGSDSYALFLYPANGLQFLGTRPKESYNVQLQLPARVGFCRGEADDLKSEGP
  YFSLTSTEQSVKNLYQLSNLGIPGVWAFHIGSTSPLDNVRPAAVGDLSAAHSSVPLGRSF
  SHATALESDYNEDNLDYYDVNEEEAEYLPGEPEEALNGHSSIDVSFQSKVDTKPLEESST
  LDPHTKEGTSLGEVGGPDLKGQVEPWDERETRSPAPPEVDRDSLAPSWETPPPYPENGSI
  QPYPDGGPVPSEMDVPPAHPEEEIVLRSYPASGHTTPLSRGTYEVGLEDNIGSNTEVFTY
  NAANKETCEHNHRQCSRHAFCTDYATGFCCHCQSKFYGNGKHCLPEGAPHRVNGKVSGHL
  HVGHTPVHFTDVDLHAYIVGNDGRAYTAISHIPQPAAQALLPLTPIGGLFGWLFALEKPG
  SENGFSLAGAAFTHDMEVTFYPGEETVRITQTAEGLDPENYLSIKTNIQGQVPYVSANFT
  AHISPYKELYHYSDSTVTSTSSRDYSLTFGAINQTWSYRIHQNITYQVCRHAPRHPSFPT
  TQQLNVDRVFALYNDEERVLRFAVTNQIGPVKEDSDPTPGNPCYDGSHMCDTTARCHPGT
  GVDYTCECASGYQGDGRNCVDENECATGFHRCGPNSVCINLPGSYRCECRSGYEFADDRH
  TCILITPPANPCEDGSHTCAPAGQARCVHHGGSTFSCACLPGYAGDGHQCTDVDECSENR
  CHPAATCYNTPGSFSCRCQPGYYGDGFQCIPDSTSSLTPCEQQQRHAQAQYAYPGARFHI
  PQCDEQGNFLPLQCHGSTGFCWCVDPDGHEVPGTQTPPGSTPPHCGPSPEPTQRPPTICE
  RWRENLLEHYGGTPRDDQYVPQCDDLGHFIPLQCHGKSDFCWCVDKDGREVQGTRSQPGT
  TPACIPTVAPPMVRPTPRPDVTPPSVGTFLLYTQGQQIGYLPLNGTRLQKDAAKTLLSLH
  GSIIVGIDYDCRERMVYWTDVAGRTISRAGLELGAEPETIVNSGLISPEGLAIDHIRRTM
  YWTDSVLDKIESALLDGSERKVLFYTDLVNPRAIAVDPIRGNLYWTDWNREAPKIETSSL
  DGENRRILINTDIGLPNGLTFDPFSKLLCWADAGTKKLECTLPDGTGRRVIQNNLKYPFS
  IVSYADHFYHTDWRRDGVVSVNKHSGQFTDEYLPEQRSHLYGITAVYPYCPTGRK

• Sequence length: 1375

Pathways

KEGG:

Cytoskeleton in muscle cells

Reactome:

Laminin interactions