FHL3 (four and a half LIM domains 3)

Gene

• Entrez ID: 2275
• Gene name: Four and a half LIM domains 3
• Gene symbol: FHL3
• Synonyms (NCBI Gene): SLIM2
• Chromosome: 1
• Chromosome location: 1p34.3
• Summary: The protein encoded by this gene is a member of a family of proteins containing a four-and-a-half LIM domain, which is a highly conserved double zinc finger motif. The encoded protein has been shown to interact with the cancer developmental regulators SMA

miRNA

miRTarBase ID	miRNA	Experiments	Reference
MIRT050493	hsa-miR-20a-5p	CLASH	23622248
MIRT044705	hsa-miR-320a	CLASH	23622248
MIRT736028	hsa-miR-663a	Luciferase reporter assay, Western blotting, qRT-PCR	33000682
MIRT2533420	hsa-miR-1233	CLIP-seq
MIRT2533421	hsa-miR-1276	CLIP-seq
MIRT2533422	hsa-miR-142-5p	CLIP-seq
MIRT2533423	hsa-miR-2110	CLIP-seq
MIRT2533424	hsa-miR-3150a-3p	CLIP-seq
MIRT2533425	hsa-miR-3175	CLIP-seq
MIRT2533426	hsa-miR-340	CLIP-seq
MIRT2533427	hsa-miR-3936	CLIP-seq
MIRT2533428	hsa-miR-4260	CLIP-seq
MIRT2533429	hsa-miR-4311	CLIP-seq
MIRT2533430	hsa-miR-4443	CLIP-seq
MIRT2533431	hsa-miR-4464	CLIP-seq
MIRT2533432	hsa-miR-4477b	CLIP-seq
MIRT2533433	hsa-miR-4721	CLIP-seq
MIRT2533434	hsa-miR-4748	CLIP-seq
MIRT2533435	hsa-miR-4777-5p	CLIP-seq
MIRT2533436	hsa-miR-518d-5p	CLIP-seq
MIRT2533437	hsa-miR-519b-5p	CLIP-seq
MIRT2533438	hsa-miR-519c-5p	CLIP-seq
MIRT2533439	hsa-miR-520c-5p	CLIP-seq
MIRT2533440	hsa-miR-526a	CLIP-seq
MIRT2533441	hsa-miR-583	CLIP-seq
MIRT2533442	hsa-miR-601	CLIP-seq
MIRT2533443	hsa-miR-939	CLIP-seq
MIRT2533421	hsa-miR-1276	CLIP-seq
MIRT2533422	hsa-miR-142-5p	CLIP-seq
MIRT2533426	hsa-miR-340	CLIP-seq
MIRT2533427	hsa-miR-3936	CLIP-seq
MIRT2533429	hsa-miR-4311	CLIP-seq
MIRT2533430	hsa-miR-4443	CLIP-seq
MIRT2533431	hsa-miR-4464	CLIP-seq
MIRT2533432	hsa-miR-4477b	CLIP-seq
MIRT2533433	hsa-miR-4721	CLIP-seq
MIRT2533434	hsa-miR-4748	CLIP-seq
MIRT2533435	hsa-miR-4777-5p	CLIP-seq
MIRT2533441	hsa-miR-583	CLIP-seq

Gene ontology (GO)

GO ID	Ontology	Definition	Evidence	Reference
GO:0001725	Component	Stress fiber	IBA
GO:0001725	Component	Stress fiber	IEA
GO:0003712	Function	Transcription coregulator activity	IBA
GO:0003779	Function	Actin binding	IBA
GO:0003779	Function	Actin binding	IEA
GO:0005515	Function	Protein binding	IPI	16189514, 21516116, 25416956, 25910212, 26871637, 27107012, 28514442, 29892012, 31515488, 32296183, 32814053, 33961781
GO:0005634	Component	Nucleus	IBA
GO:0005634	Component	Nucleus	IEA
GO:0005737	Component	Cytoplasm	IEA
GO:0005925	Component	Focal adhesion	HDA	21423176
GO:0007517	Process	Muscle organ development	TAS	10049693
GO:0008270	Function	Zinc ion binding	IEA
GO:0030018	Component	Z disc	IBA
GO:0030018	Component	Z disc	IEA
GO:0030036	Process	Actin cytoskeleton organization	IBA
GO:0030036	Process	Actin cytoskeleton organization	IEA
GO:0046872	Function	Metal ion binding	IEA

Other IDs

• MIM: 602790
• HGNC: 3704
• e!Ensembl: ENSG00000183386

Protein

• UniProt ID: Q13643
• Protein name: Four and a half LIM domains protein 3 (FHL-3) (Skeletal muscle LIM-protein 2) (SLIM-2)
• Protein function: Recruited by SOX15 to FOXK1 promoters where it acts as a transcriptional coactivator of FOXK1.
• PDB: 1WYH, 2CUQ, 2EHE
• Family and domains:

  Pfam

  Accession	ID	Position in sequence	Description	Type
  PF00412	LIM	40 → 97	LIM domain	Domain
  PF00412	LIM	101 → 158	LIM domain	Domain
  PF00412	LIM	162 → 217	LIM domain	Domain
  PF00412	LIM	221 → 279	LIM domain	Domain

• Tissue specificity: TISSUE SPECIFICITY: Expressed only in skeletal muscle.
• Sequence:

  MSESFDCAKCNESLYGRKYIQTDSGPYCVPCYDNTFANTCAECQQLIGHDSRELFYEDRH
  FHEGCFRCCRCQRSLADEPFTCQDSELLCNDCYCSAFSSQCSACGETVMPGSRKLEYGGQ
  TWHEHCFLCSGCEQPLGSRSFVPDKGAHYCVPCYENKFAPRCARCSKTLTQGGVTYRDQP
  WHRECLVCTGCQTPLAGQQFTSRDEDPYCVACFGELFAPKCSSCKRPIVGLGGGKYVSFE
  DRHWHHNCFSCARCSTSLVGQGFVPDGDQVLCQGCSQAGP

• Sequence length: 280

Pathways

KEGG:

Cytoskeleton in muscle cells