TWF2 (twinfilin actin binding protein 2)

Gene

• Entrez ID: 11344
• Gene name: Twinfilin actin binding protein 2
• Gene symbol: TWF2
• Synonyms (NCBI Gene): A6RP, A6r, MSTP011, PTK9L
• Chromosome: 3
• Chromosome location: 3p21.2
• Summary: The protein encoded by this gene was identified by its interaction with the catalytic domain of protein kinase C-zeta. The encoded protein contains an actin-binding site and an ATP-binding site. It is most closely related to twinfilin (PTK9), a conserved

miRNA

miRTarBase ID	miRNA	Experiments	Reference
MIRT005539	hsa-miR-1-3p	Luciferase reporter assay, Microarray, Northern blot, qRT-PCR, Western blot	20571053
MIRT005540	hsa-miR-126-3p	Luciferase reporter assay, Microarray, Northern blot, qRT-PCR, Western blot	20571053
MIRT042297	hsa-miR-484	CLASH	23622248
MIRT2139695	hsa-miR-1207-3p	CLIP-seq
MIRT2139696	hsa-miR-1207-5p	CLIP-seq
MIRT2139697	hsa-miR-2276	CLIP-seq
MIRT2139698	hsa-miR-3120-3p	CLIP-seq
MIRT2139699	hsa-miR-4763-3p	CLIP-seq
MIRT2653620	hsa-miR-1206	CLIP-seq
MIRT2653621	hsa-miR-1285	CLIP-seq
MIRT2653622	hsa-miR-1587	CLIP-seq
MIRT2653623	hsa-miR-3187-5p	CLIP-seq
MIRT2653624	hsa-miR-3649	CLIP-seq
MIRT2653625	hsa-miR-378g	CLIP-seq
MIRT2653626	hsa-miR-3926	CLIP-seq
MIRT2653627	hsa-miR-3943	CLIP-seq
MIRT2653628	hsa-miR-4286	CLIP-seq
MIRT2653629	hsa-miR-4313	CLIP-seq
MIRT2653630	hsa-miR-4314	CLIP-seq
MIRT2653631	hsa-miR-4474-3p	CLIP-seq
MIRT2653632	hsa-miR-4474-5p	CLIP-seq
MIRT2653633	hsa-miR-4486	CLIP-seq
MIRT2653634	hsa-miR-4492	CLIP-seq
MIRT2653635	hsa-miR-4498	CLIP-seq
MIRT2653636	hsa-miR-4656	CLIP-seq
MIRT2653637	hsa-miR-4675	CLIP-seq
MIRT2653638	hsa-miR-4707-5p	CLIP-seq
MIRT2653639	hsa-miR-4731-5p	CLIP-seq
MIRT2653640	hsa-miR-4732-3p	CLIP-seq
MIRT2653641	hsa-miR-4741	CLIP-seq
MIRT2653642	hsa-miR-4755-3p	CLIP-seq
MIRT2653643	hsa-miR-548s	CLIP-seq
MIRT2653644	hsa-miR-578	CLIP-seq
MIRT2653645	hsa-miR-581	CLIP-seq
MIRT2653646	hsa-miR-612	CLIP-seq
MIRT2653647	hsa-miR-663b	CLIP-seq
MIRT2653648	hsa-miR-762	CLIP-seq
MIRT2653620	hsa-miR-1206	CLIP-seq
MIRT2139696	hsa-miR-1207-5p	CLIP-seq
MIRT2653621	hsa-miR-1285	CLIP-seq
MIRT2653622	hsa-miR-1587	CLIP-seq
MIRT2653623	hsa-miR-3187-5p	CLIP-seq
MIRT2694513	hsa-miR-3194-5p	CLIP-seq
MIRT2653624	hsa-miR-3649	CLIP-seq
MIRT2653625	hsa-miR-378g	CLIP-seq
MIRT2653626	hsa-miR-3926	CLIP-seq
MIRT2653627	hsa-miR-3943	CLIP-seq
MIRT2653628	hsa-miR-4286	CLIP-seq
MIRT2653629	hsa-miR-4313	CLIP-seq
MIRT2653630	hsa-miR-4314	CLIP-seq
MIRT2653631	hsa-miR-4474-3p	CLIP-seq
MIRT2653632	hsa-miR-4474-5p	CLIP-seq
MIRT2653633	hsa-miR-4486	CLIP-seq
MIRT2653634	hsa-miR-4492	CLIP-seq
MIRT2653635	hsa-miR-4498	CLIP-seq
MIRT2694514	hsa-miR-4514	CLIP-seq
MIRT2694515	hsa-miR-4645-5p	CLIP-seq
MIRT2653636	hsa-miR-4656	CLIP-seq
MIRT2694516	hsa-miR-4673	CLIP-seq
MIRT2653637	hsa-miR-4675	CLIP-seq
MIRT2694517	hsa-miR-4692	CLIP-seq
MIRT2653638	hsa-miR-4707-5p	CLIP-seq
MIRT2653639	hsa-miR-4731-5p	CLIP-seq
MIRT2653640	hsa-miR-4732-3p	CLIP-seq
MIRT2653641	hsa-miR-4741	CLIP-seq
MIRT2653642	hsa-miR-4755-3p	CLIP-seq
MIRT2139699	hsa-miR-4763-3p	CLIP-seq
MIRT2653643	hsa-miR-548s	CLIP-seq
MIRT2653644	hsa-miR-578	CLIP-seq
MIRT2653645	hsa-miR-581	CLIP-seq
MIRT2653646	hsa-miR-612	CLIP-seq
MIRT2653647	hsa-miR-663b	CLIP-seq
MIRT2653648	hsa-miR-762	CLIP-seq
MIRT2694518	hsa-miR-940	CLIP-seq

Gene ontology (GO)

GO ID	Ontology	Definition	Evidence	Reference
GO:0003723	Function	RNA binding	HDA	22658674
GO:0003779	Function	Actin binding	IEA
GO:0003785	Function	Actin monomer binding	IBA
GO:0003785	Function	Actin monomer binding	IEA
GO:0003785	Function	Actin monomer binding	ISS	12807912, 18837697
GO:0005080	Function	Protein kinase C binding	IPI	10406962
GO:0005515	Function	Protein binding	IPI	21044950
GO:0005524	Function	ATP binding	IDA	10406962
GO:0005546	Function	Phosphatidylinositol-4,5-bisphosphate binding	IEA
GO:0005546	Function	Phosphatidylinositol-4,5-bisphosphate binding	ISS	12807912
GO:0005737	Component	Cytoplasm	IBA
GO:0005737	Component	Cytoplasm	IDA	25468996
GO:0005737	Component	Cytoplasm	IEA
GO:0005737	Component	Cytoplasm	NAS	10406962
GO:0005856	Component	Cytoskeleton	IEA
GO:0005884	Component	Actin filament	IBA
GO:0010591	Process	Regulation of lamellipodium assembly	IBA
GO:0010592	Process	Positive regulation of lamellipodium assembly	IMP	17910947
GO:0010976	Process	Positive regulation of neuron projection development	IBA
GO:0010976	Process	Positive regulation of neuron projection development	IMP	17910947
GO:0030016	Component	Myofibril	IBA
GO:0030016	Component	Myofibril	ISS	18837697
GO:0030027	Component	Lamellipodium	IEA
GO:0030027	Component	Lamellipodium	ISS	12807912
GO:0030030	Process	Cell projection organization	IEA
GO:0030042	Process	Actin filament depolymerization	IBA
GO:0030175	Component	Filopodium	IEA
GO:0030175	Component	Filopodium	ISS	12807912
GO:0030426	Component	Growth cone	IDA	17910947
GO:0030837	Process	Negative regulation of actin filament polymerization	IEA
GO:0030837	Process	Negative regulation of actin filament polymerization	ISS	12807912
GO:0032420	Component	Stereocilium	IEA
GO:0032420	Component	Stereocilium	ISS	19955359
GO:0032532	Process	Regulation of microvillus length	IC	19955359
GO:0032956	Process	Regulation of actin cytoskeleton organization	IMP	17910947
GO:0042995	Component	Cell projection	IEA
GO:0043168	Function	Anion binding	IEA
GO:0045296	Function	Cadherin binding	HDA	25468996
GO:0045773	Process	Positive regulation of axon extension	IMP	17910947
GO:0048471	Component	Perinuclear region of cytoplasm	IEA
GO:0048471	Component	Perinuclear region of cytoplasm	ISS	12807912
GO:0051015	Function	Actin filament binding	IBA
GO:0051016	Process	Barbed-end actin filament capping	IBA
GO:0051016	Process	Barbed-end actin filament capping	ISS	18837697
GO:0070062	Component	Extracellular exosome	HDA	19199708
GO:0071300	Process	Cellular response to retinoic acid	IMP	17910947
GO:0071363	Process	Cellular response to growth factor stimulus	IMP	17910947

Other IDs

• MIM: 607433
• HGNC: 9621
• e!Ensembl: ENSG00000247596

Protein

• UniProt ID: Q6IBS0
• Protein name: Twinfilin-2 (A6-related protein) (hA6RP) (Protein tyrosine kinase 9-like) (Twinfilin-1-like protein)
• Protein function: Actin-binding protein involved in motile and morphological processes. Inhibits actin polymerization, likely by sequestering G-actin. By capping the barbed ends of filaments, it also regulates motility. Seems to play an important role in clathrin
• PDB: 2VAC, 2W0I
• Family and domains:

  Pfam

  Accession	ID	Position in sequence	Description	Type
  PF00241	Cofilin_ADF	9 → 137	Cofilin/tropomyosin-type actin-binding protein	Domain
  PF00241	Cofilin_ADF	186 → 311	Cofilin/tropomyosin-type actin-binding protein	Domain

• Tissue specificity: TISSUE SPECIFICITY: Ubiquitously expressed (at protein level). {ECO:0000269|PubMed:10406962}.
• Sequence:

  MAHQTGIHATEELKEFFAKARAGSVRLIKVVIEDEQLVLGASQEPVGRWDQDYDRAVLPL
  LDAQQPCYLLYRLDSQNAQGFEWLFLAWSPDNSPVRLKMLYAATRATVKKEFGGGHIKDE
  LFGTVKDDLSFAGYQKHLSSCAAPAPLTSAERELQQIRINEVKTEISVESKHQTLQGLAF
  PLQPEAQRALQQLKQKMVNYIQMKLDLERETIELVHTEPTDVAQLPSRVPRDAARYHFFL
  YKHTHEGDPLESVVFIYSMPGYKCSIKERMLYSSCKSRLLDSVEQDFHLEIAKKIEIGDG
  AELTAEFLYDEVHPKQHAFKQAFAKPKGPGGKRGHKRLIRGPGENGDDS

• Sequence length: 349