ME3 (malic enzyme 3)

Gene

• Entrez ID: 10873
• Gene name: Malic enzyme 3
• Gene symbol: ME3
• Synonyms (NCBI Gene): NADP-ME
• Chromosome: 11
• Chromosome location: 11q14.2
• Summary: Malic enzyme catalyzes the oxidative decarboxylation of malate to pyruvate using either NAD+ or NADP+ as a cofactor. Mammalian tissues contain 3 distinct isoforms of malic enzyme: a cytosolic NADP(+)-dependent isoform, a mitochondrial NADP(+)-dependent is

miRNA

miRTarBase ID	miRNA	Experiments	Reference
MIRT028648	hsa-miR-30a-5p	Proteomics	18668040

Gene ontology (GO)

GO ID	Ontology	Definition	Evidence	Reference
GO:0004470	Function	Malic enzyme activity	IDA	7818469
GO:0004470	Function	Malic enzyme activity	IEA
GO:0004473	Function	Malate dehydrogenase (decarboxylating) (NADP+) activity	IBA
GO:0004473	Function	Malate dehydrogenase (decarboxylating) (NADP+) activity	IDA	7818469
GO:0004473	Function	Malate dehydrogenase (decarboxylating) (NADP+) activity	IEA
GO:0005739	Component	Mitochondrion	HTP	34800366
GO:0005739	Component	Mitochondrion	IBA
GO:0005739	Component	Mitochondrion	IEA
GO:0005739	Component	Mitochondrion	TAS	7818469
GO:0005759	Component	Mitochondrial matrix	IEA
GO:0005759	Component	Mitochondrial matrix	IEA
GO:0005759	Component	Mitochondrial matrix	TAS
GO:0006090	Process	Pyruvate metabolic process	IBA
GO:0006108	Process	Malate metabolic process	IBA
GO:0006108	Process	Malate metabolic process	IDA	7818469
GO:0006108	Process	Malate metabolic process	IEA
GO:0008948	Function	Oxaloacetate decarboxylase activity	IEA
GO:0016491	Function	Oxidoreductase activity	IEA
GO:0016616	Function	Oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor	IEA
GO:0042866	Process	Pyruvate biosynthetic process	IDA	7818469
GO:0046872	Function	Metal ion binding	IEA
GO:0050661	Function	NADP binding	IEA
GO:0051287	Function	NAD binding	IEA
GO:0070401	Function	NADP+ binding	IDA	7818469

Other IDs

• MIM: 604626
• HGNC: 6985
• e!Ensembl: ENSG00000151376

Protein

• UniProt ID: Q16798
• Protein name: NADP-dependent malic enzyme, mitochondrial (NADP-ME) (EC 1.1.1.40) (Malic enzyme 3)
• Protein function: Catalyzes the oxidative decarboxylation of (S)-malate to pyruvate using NADP(+) as a cofactor (PubMed:7818469). Can also reverse the decarboxylation reaction, but only with significantly lower efficiency (PubMed:7818469). {ECO:0000269|PubMed:781
• PDB: 8E76, 8E78, 8E8O, 8EYN, 8EYO
• Family and domains:

  Pfam

  Accession	ID	Position in sequence	Description	Type
  PF00390	malic	114 → 295	Malic enzyme, N-terminal domain	Domain
  PF03949	Malic_M	305 → 557	Malic enzyme, NAD binding domain	Domain

• Tissue specificity: TISSUE SPECIFICITY: Expressed predominantly in organs with a low-division rate.
• Sequence:

  MGAALGTGTRLAPWPGRACGALPRWTPTAPAQGCHSKPGPARPVPLKKRGYDVTRNPHLN
  KGMAFTLEERLQLGIHGLIPPCFLSQDVQLLRIMRYYERQQSDLDKYIILMTLQDRNEKL
  FYRVLTSDVEKFMPIVYTPTVGLACQHYGLTFRRPRGLFITIHDKGHLATMLNSWPEDNI
  KAVVVTDGERILGLGDLGCYGMGIPVGKLALYTACGGVNPQQCLPVLLDVGTNNEELLRD
  PLYIGLKHQRVHGKAYDDLLDEFMQAVTDKFGINCLIQFEDFANANAFRLLNKYRNKYCM
  FNDDIQGTASVAVAGILAALRITKNKLSNHVFVFQGAGEAAMGIAHLLVMALEKEGVPKA
  EATRKIWMVDSKGLIVKGRSHLNHEKEMFAQDHPEVNSLEEVVRLVKPTAIIGVAAIAGA
  FTEQILRDMASFHERPIIFALSNPTSKAECTAEKCYRVTEGRGIFASGSPFKSVTLEDGK
  TFIPGQGNNAYVFPGVALGVIAGGIRHIPDEIFLLTAEQIAQEVSEQHLSQGRLYPPLST
  IRDVSLRIAIKVLDYAYKHNLASYYPEPKDKEAFVRSLVYTPDYDSFTLDSYTWPKEAMN
  VQTV

• Sequence length: 604

Pathways

KEGG:

Pyruvate metabolism
Metabolic pathways
Carbon metabolism
PPAR signaling pathway

Reactome:

Citric acid cycle (TCA cycle)