NES (nestin)

Gene

• Entrez ID: 10763
• Gene name: Nestin
• Gene symbol: NES
• Synonyms (NCBI Gene): Nbla00170
• Chromosome: 1
• Chromosome location: 1q23.1
• Summary: This gene encodes a member of the intermediate filament protein family and is expressed primarily in nerve cells. [provided by RefSeq, Sep 2011]

miRNA

miRTarBase ID	miRNA	Experiments	Reference
MIRT020595	hsa-miR-155-5p	Proteomics	18668040
MIRT035540	hsa-miR-125b-5p	Luciferase reporter assay	23016664
MIRT035540	hsa-miR-125b-5p	Luciferase reporter assay	23016664
MIRT035540	hsa-miR-125b-5p	Luciferase reporter assay	23016664
MIRT438696	hsa-miR-432-5p	Flow, Immunocytochemistry, Luciferase reporter assay, qRT-PCR, Western blot	24657437
MIRT438696	hsa-miR-432-5p	Flow, Immunocytochemistry, Luciferase reporter assay, qRT-PCR, Western blot	24657437
MIRT438696	hsa-miR-432-5p	Flow, Immunocytochemistry, Luciferase reporter assay, qRT-PCR, Western blot	24657437
MIRT438696	hsa-miR-432-5p	Flow, Immunocytochemistry, Luciferase reporter assay, qRT-PCR, Western blot	24657437
MIRT756178	hsa-miR-4488	Luciferase reporter assay, Western blotting, qRT-PCR, Immunohistochemistry (IHC)	38008717
MIRT756179	hsa-miR-4443	Luciferase reporter assay, Western blotting, qRT-PCR, Immunohistochemistry (IHC)	38008717
MIRT1180674	hsa-miR-1909	CLIP-seq
MIRT1180675	hsa-miR-204	CLIP-seq
MIRT1180676	hsa-miR-211	CLIP-seq
MIRT1180677	hsa-miR-3121-5p	CLIP-seq
MIRT1180678	hsa-miR-3689a-3p	CLIP-seq
MIRT1180679	hsa-miR-3689c	CLIP-seq
MIRT1180680	hsa-miR-3938	CLIP-seq
MIRT1180681	hsa-miR-4728-5p	CLIP-seq
MIRT1180682	hsa-miR-4755-5p	CLIP-seq
MIRT1180683	hsa-miR-623	CLIP-seq

Transcription factors

Transcription factor	Regulation	Reference
MYCN	Activation	15117961
SOX10	Unknown	19845757
SOX9	Unknown	19845757

Gene ontology (GO)

GO ID	Ontology	Definition	Evidence	Reference
GO:0000086	Process	G2/M transition of mitotic cell cycle	IDA	19384922
GO:0005515	Function	Protein binding	IPI	26496610, 28514442, 30021884, 33961781, 35271311
GO:0005737	Component	Cytoplasm	IDA	19384922
GO:0005737	Component	Cytoplasm	IEA
GO:0005882	Component	Intermediate filament	IBA
GO:0005882	Component	Intermediate filament	IEA
GO:0005882	Component	Intermediate filament	NAS	9917366
GO:0007399	Process	Nervous system development	IEA
GO:0007399	Process	Nervous system development	IEA
GO:0007417	Process	Central nervous system development	NAS	9917366
GO:0007420	Process	Brain development	ISS
GO:0019215	Function	Intermediate filament binding	IEA
GO:0019215	Function	Intermediate filament binding	ISS
GO:0030844	Process	Positive regulation of intermediate filament depolymerization	IBA
GO:0030844	Process	Positive regulation of intermediate filament depolymerization	ISS
GO:0031076	Process	Embryonic camera-type eye development	ISS
GO:0031730	Function	CCR5 chemokine receptor binding	IBA
GO:0032091	Process	Negative regulation of protein binding	IDA	21185309
GO:0043086	Process	Negative regulation of catalytic activity	IDA	21185309
GO:0043524	Process	Negative regulation of neuron apoptotic process	IEA
GO:0045111	Component	Intermediate filament cytoskeleton	IDA
GO:0048858	Process	Cell projection morphogenesis	IEA
GO:0051402	Process	Neuron apoptotic process	IEA
GO:0072089	Process	Stem cell proliferation	IMP	19384922
GO:2000179	Process	Positive regulation of neural precursor cell proliferation	ISS

Other IDs

• MIM: 600915
• HGNC: 7756
• e!Ensembl: ENSG00000132688

Protein

• UniProt ID: P48681
• Protein name: Nestin
• Protein function: Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter
• Family and domains:

  Pfam

  Accession	ID	Position in sequence	Description	Type
  PF00038	Filament	7 → 160	Intermediate filament protein	Coiled-coil
  PF00038	Filament	178 → 312	Intermediate filament protein	Coiled-coil

• Tissue specificity: TISSUE SPECIFICITY: CNS stem cells.
• Sequence:

  MEGCMGEESFQMWELNRRLEAYLARVKALEEQNELLSAELGGLRAQSADTSWRAHADDEL
  AALRALVDQRWREKHAAEVARDNLAEELEGVAGRCQQLRLARERTTEEVARNRRAVEAEK
  CARAWLSSQVAELERELEALRVAHEEERVGLNAQAACAPRCPAPPRGPPAPAPEVEELAR
  RLGEAWRGAVRGYQERVAHMETSLGQARERLGRAVQGAREGRLELQQLQAERGGLLERRA
  ALEQRLEGRWQERLRATEKFQLAVEALEQEKQGLQSQIAQVLEGRQQLAHLKMSLSLEVA
  TYRTLLEAENSRLQTPGGGSKTSLSFQDPKLELQFPRTPEGRRLGSLLPVLSPTSLPSPL
  PATLETPVPAFLKNQEFLQARTPTLASTPIPPTPQAPSPAVDAEIRAQDAPLSLLQTQGG
  RKQAPEPLRAEARVAIPASVLPGPEEPGGQRQEASTGQSPEDHASLAPPLSPDHSSLEAK
  DGESGGSRVFSICRGEGEGQIWGLVEKETAIEGKVVSSLQQEIWEEEDLNRKEIQDSQVP
  LEKETLKSLGEEIQESLKTLENQSHETLERENQECPRSLEEDLETLKSLEKENKELLKDV
  EVVRPLEKEAVGQLKPTGKEDTQTLQSLQKENQELMKSLEGNLETFLFPGTENQELVSSL
  QENLESLTALEKENQEPLRSPEVGDEEALRPLTKENQEPLRSLEDENKEAFRSLEKENQE
  PLKTLEEEDQSIVRPLETENHKSLRSLEEQDQETLRTLEKETQQRRRSLGEQDQMTLRPP
  EKVDLEPLKSLDQEIARPLENENQEFLKSLKEESVEAVKSLETEILESLKSAGQENLETL
  KSPETQAPLWTPEEINQGAMNPLEKEIQEPLESVEVNQETFRLLEEENQESLRSLGAWNL
  ENLRSPEEVDKESQRNLEEEENLGKGEYQESLRSLEEEGQELPQSADVQRWEDTVEKDQE
  LAQESPPGMAGVENEDEAELNLREQDGFTGKEEVVEQGELNATEEVWIPGEGHPESPEPK
  EQRGLVEGASVKGGAEGLQDPEGQSQQVGAPGLQAPQGLPEAIEPLVEDDVAPGGDQASP
  EVMLGSEPAMGESAAGAEPGPGQGVGGLGDPGHLTREEVMEPPLEEESLEAKRVQGLEGP
  RKDLEEAGGLGTEFSELPGKSRDPWEPPREGREESEAEAPRGAEEAFPAETLGHTGSDAP
  SPWPLGSEEAEEDVPPVLVSPSPTYTPILEDAPGPQPQAEGSQEASWGVQGRAEALGKVE
  SEQEELGSGEIPEGPQEEGEESREESEEDELGETLPDSTPLGFYLRSPTSPRWDPTGEQR
  PPPQGETGKEGWDPAVLASEGLEAPPSEKEEGEEGEEECGRDSDLSEEFEDLGTEAPFLP
  GVPGEVAEPLGQVPQLLLDPAAWDRDGESDGFADEEESGEEGEEDQEEGREPGAGRWGPG
  SSVGSLQALSSSQRGEFLESDSVSVSVPWDDSLRGAVAGAPKTALETESQDSAEPSGSEE
  ESDPVSLEREDKVPGPLEIPSGMEDAGPGADIIGVNGQGPNLEGKSQHVNGGVMNGLEQS
  EEVGQGMPLVSEGDRGSPFQEEEGSALKTSWAGAPVHLGQGQFLKFTQREGDRESWSSGE
  D

• Sequence length: 1621

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