MED30 (mediator complex subunit 30)

Gene

• Entrez ID: 90390
• Gene name: Mediator complex subunit 30
• Gene symbol: MED30
• Synonyms (NCBI Gene): MED30S, THRAP6, TRAP25
• Chromosome: 8
• Chromosome location: 8q24.11
• Summary: The multiprotein TRAP/Mediator complex facilitates gene expression through a wide variety of transcriptional activators. MED30 is a component of this complex that appears to be metazoan specific (Baek et al., 2002 [PubMed 11909976]).[supplied by OMIM, Nov

miRNA

miRTarBase ID	miRNA	Experiments	Reference
MIRT450262	hsa-miR-5584-5p	PAR-CLIP	22100165
MIRT450263	hsa-miR-3126-5p	PAR-CLIP	22100165
MIRT450261	hsa-miR-6875-5p	PAR-CLIP	22100165
MIRT450260	hsa-miR-4419a	PAR-CLIP	22100165
MIRT450259	hsa-miR-4510	PAR-CLIP	22100165
MIRT450258	hsa-miR-6127	PAR-CLIP	22100165
MIRT450257	hsa-miR-6129	PAR-CLIP	22100165
MIRT450256	hsa-miR-6130	PAR-CLIP	22100165
MIRT450255	hsa-miR-6133	PAR-CLIP	22100165
MIRT450254	hsa-miR-3148	PAR-CLIP	22100165
MIRT450252	hsa-miR-4668-5p	PAR-CLIP	22100165
MIRT450253	hsa-miR-513a-5p	PAR-CLIP	22100165
MIRT450251	hsa-miR-27a-3p	PAR-CLIP	22100165
MIRT450250	hsa-miR-27b-3p	PAR-CLIP	22100165
MIRT450249	hsa-miR-6834-5p	PAR-CLIP	22100165
MIRT450248	hsa-miR-1226-5p	PAR-CLIP	22100165
MIRT450247	hsa-miR-6734-5p	PAR-CLIP	22100165
MIRT450262	hsa-miR-5584-5p	PAR-CLIP	22100165
MIRT450263	hsa-miR-3126-5p	PAR-CLIP	22100165
MIRT450261	hsa-miR-6875-5p	PAR-CLIP	22100165
MIRT450260	hsa-miR-4419a	PAR-CLIP	22100165
MIRT450259	hsa-miR-4510	PAR-CLIP	22100165
MIRT450258	hsa-miR-6127	PAR-CLIP	22100165
MIRT450257	hsa-miR-6129	PAR-CLIP	22100165
MIRT450256	hsa-miR-6130	PAR-CLIP	22100165
MIRT450255	hsa-miR-6133	PAR-CLIP	22100165
MIRT450254	hsa-miR-3148	PAR-CLIP	22100165
MIRT450252	hsa-miR-4668-5p	PAR-CLIP	22100165
MIRT450253	hsa-miR-513a-5p	PAR-CLIP	22100165
MIRT450251	hsa-miR-27a-3p	PAR-CLIP	22100165
MIRT450250	hsa-miR-27b-3p	PAR-CLIP	22100165
MIRT450249	hsa-miR-6834-5p	PAR-CLIP	22100165
MIRT450248	hsa-miR-1226-5p	PAR-CLIP	22100165
MIRT450247	hsa-miR-6734-5p	PAR-CLIP	22100165
MIRT1141715	hsa-miR-1179	CLIP-seq
MIRT1141716	hsa-miR-27a	CLIP-seq
MIRT1141717	hsa-miR-27b	CLIP-seq
MIRT1141718	hsa-miR-3163	CLIP-seq
MIRT1141719	hsa-miR-412	CLIP-seq
MIRT1141720	hsa-miR-4778-3p	CLIP-seq
MIRT1141721	hsa-miR-577	CLIP-seq
MIRT1141716	hsa-miR-27a	CLIP-seq
MIRT1141717	hsa-miR-27b	CLIP-seq

Gene ontology (GO)

GO ID	Ontology	Definition	Evidence	Reference
GO:0000151	Component	Ubiquitin ligase complex	IEA
GO:0003712	Function	Transcription coregulator activity	IBA
GO:0003712	Function	Transcription coregulator activity	IDA	10198638
GO:0003713	Function	Transcription coactivator activity	IDA	12218053
GO:0003713	Function	Transcription coactivator activity	NAS	10235266
GO:0005515	Function	Protein binding	IPI	21784977, 24981860, 25416956, 32296183, 32814053, 33961781, 35271311
GO:0005634	Component	Nucleus	IDA	10235267, 24882805
GO:0005634	Component	Nucleus	IEA
GO:0005654	Component	Nucleoplasm	TAS
GO:0016567	Process	Protein ubiquitination	IEA
GO:0016592	Component	Mediator complex	IBA
GO:0016592	Component	Mediator complex	IDA	10198638
GO:0016592	Component	Mediator complex	IEA
GO:0032968	Process	Positive regulation of transcription elongation by RNA polymerase II	NAS	11559591
GO:0035019	Process	Somatic stem cell population maintenance	IEA
GO:0042809	Function	Nuclear vitamin D receptor binding	NAS	10235266
GO:0045893	Process	Positive regulation of DNA-templated transcription	IBA
GO:0045893	Process	Positive regulation of DNA-templated transcription	IDA	10198638
GO:0046966	Function	Nuclear thyroid hormone receptor binding	IDA	10198638
GO:0051123	Process	RNA polymerase II preinitiation complex assembly	NAS	11559591
GO:0060261	Process	Positive regulation of transcription initiation by RNA polymerase II	IDA	12218053
GO:0060261	Process	Positive regulation of transcription initiation by RNA polymerase II	NAS	11559591
GO:0061630	Function	Ubiquitin protein ligase activity	IEA
GO:0070847	Component	Core mediator complex	IPI	24882805

Other IDs

• MIM: 610237
• HGNC: 23032
• e!Ensembl: ENSG00000164758

Protein

• UniProt ID: Q96HR3
• Protein name: Mediator of RNA polymerase II transcription subunit 30 (Mediator complex subunit 30) (TRAP/Mediator complex component TRAP25) (Thyroid hormone receptor-associated protein 6) (Thyroid hormone receptor-associated protein complex 25 kDa component) (Trap25)
• Protein function: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RN
• PDB: 7EMF, 7ENA, 7ENC, 7ENJ, 7LBM, 7NVR, 8GXQ, 8GXS, 8T9D, 8TQW, 8TRH
• Family and domains:

  Pfam

  Accession	ID	Position in sequence	Description	Type
  PF11315	Med30	29 → 176	Mediator complex subunit 30	Domain

• Tissue specificity: TISSUE SPECIFICITY: Expressed in brain, heart, kidney, liver, lung, pancreas, placenta and skeletal muscle. {ECO:0000269|PubMed:11909976}.
• Sequence:

  MSTPPLAASGMAPGPFAGPQAQQAAREVNTASLCRIGQETVQDIVYRTMEIFQLLRNMQL
  PNGVTYHTGTYQDRLTKLQDNLRQLSVLFRKLRLVYDKCNENCGGMDPIPVEQLIPYVEE
  DGSKNDDRAGPPRFASEERREIAEVNKKLKQKNQQLKQIMDQLRNLIWDINAMLAMRN

• Sequence length: 178

Pathways

KEGG:

Thyroid hormone signaling pathway

Reactome:

PPARA activates gene expression
Generic Transcription Pathway
Transcriptional regulation of white adipocyte differentiation