CA9 (carbonic anhydrase 9)

Gene

• Entrez ID: 768
• Gene name: Carbonic anhydrase 9
• Gene symbol: CA9
• Synonyms (NCBI Gene): CAIX, MN
• Chromosome: 9
• Chromosome location: 9p13.3
• Summary: Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption,

miRNA

miRTarBase ID	miRNA	Experiments	Reference
MIRT027439	hsa-miR-98-5p	Microarray	19088304
MIRT028954	hsa-miR-26b-5p	Microarray	19088304
MIRT1954468	hsa-miR-1229	CLIP-seq
MIRT1954469	hsa-miR-1245b-3p	CLIP-seq
MIRT1954470	hsa-miR-149	CLIP-seq
MIRT1954471	hsa-miR-3064-5p	CLIP-seq
MIRT1954472	hsa-miR-342-3p	CLIP-seq
MIRT1954473	hsa-miR-3652	CLIP-seq
MIRT1954474	hsa-miR-4330	CLIP-seq
MIRT1954475	hsa-miR-4430	CLIP-seq
MIRT1954476	hsa-miR-4505	CLIP-seq
MIRT1954477	hsa-miR-4794	CLIP-seq
MIRT1954468	hsa-miR-1229	CLIP-seq
MIRT1954469	hsa-miR-1245b-3p	CLIP-seq
MIRT1954470	hsa-miR-149	CLIP-seq
MIRT1954471	hsa-miR-3064-5p	CLIP-seq
MIRT1954473	hsa-miR-3652	CLIP-seq
MIRT1954474	hsa-miR-4330	CLIP-seq
MIRT1954475	hsa-miR-4430	CLIP-seq
MIRT1954476	hsa-miR-4505	CLIP-seq
MIRT1954477	hsa-miR-4794	CLIP-seq

Transcription factors

Transcription factor	Regulation	Reference
AHR	Repression	19154183
ARNT	Activation	22387692
ATF4	Unknown	19564335
EP300	Activation	16270297
EPAS1	Activation	12171890
ETS1	Repression	18381358
HDAC4	Unknown	20110259
HIF1A	Activation	12171890;19154183
HIF1A	Unknown	17486380;19238077
SP1	Activation	16270297
SP1	Unknown	12615703
SP3	Unknown	12615703
VHL	Unknown	15754010

Gene ontology (GO)

GO ID	Ontology	Definition	Evidence	Reference
GO:0001666	Process	Response to hypoxia	IEA
GO:0002009	Process	Morphogenesis of an epithelium	IEA
GO:0004089	Function	Carbonate dehydratase activity	IBA
GO:0004089	Function	Carbonate dehydratase activity	IDA	18703501, 19805286
GO:0004089	Function	Carbonate dehydratase activity	IEA
GO:0004089	Function	Carbonate dehydratase activity	IMP	17314045, 17705204, 19206230
GO:0004089	Function	Carbonate dehydratase activity	TAS	8661007
GO:0005515	Function	Protein binding	IPI	26871637, 32296183
GO:0005634	Component	Nucleus	IEA
GO:0005730	Component	Nucleolus	IEA
GO:0005886	Component	Plasma membrane	IBA
GO:0005886	Component	Plasma membrane	IDA	22087255
GO:0005886	Component	Plasma membrane	IEA
GO:0005886	Component	Plasma membrane	TAS
GO:0008270	Function	Zinc ion binding	IDA	18703501, 19805286
GO:0008270	Function	Zinc ion binding	IEA
GO:0009410	Process	Response to xenobiotic stimulus	IEA
GO:0016020	Component	Membrane	IEA
GO:0016020	Component	Membrane	TAS	8661007
GO:0016323	Component	Basolateral plasma membrane	IEA
GO:0016829	Function	Lyase activity	IEA
GO:0031528	Component	Microvillus membrane	IEA
GO:0033574	Process	Response to testosterone	IEA
GO:0042995	Component	Cell projection	IEA
GO:0046872	Function	Metal ion binding	IEA
GO:0046903	Process	Secretion	IEA
GO:0140677	Function	Molecular function activator activity	IDA	29564477

Other IDs

• MIM: 603179
• HGNC: 1383
• e!Ensembl: ENSG00000107159

Protein

• UniProt ID: Q16790
• Protein name: Carbonic anhydrase 9 (EC 4.2.1.1) (Carbonate dehydratase IX) (Carbonic anhydrase IX) (CA-IX) (CAIX) (Membrane antigen MN) (P54/58N) (Renal cell carcinoma-associated antigen G250) (RCC-associated antigen G250) (pMW1)
• Protein function: Catalyzes the interconversion between carbon dioxide and water and the dissociated ions of carbonic acid (i.e. bicarbonate and hydrogen ions). {ECO:0000269|PubMed:17314045, ECO:0000269|PubMed:17705204, ECO:0000269|PubMed:18703501, ECO:0000269|Pu
• PDB: 2HKF, 3IAI, 5DVX, 5FL4, 5FL5, 5FL6, 6FE0, 6FE1, 6FE2, 6G98, 6G9U, 6QN2, 6QN5, 6QN6, 6QUT, 6RQN, 6RQQ, 6RQU, 6RQW, 6TL5, 6TL6, 6Y74, 7POM, 8CO0, 8Q18, 8Q19, 8Q1A
• Family and domains:

  Pfam

  Accession	ID	Position in sequence	Description	Type
  PF00194	Carb_anhydrase	140 → 389	Eukaryotic-type carbonic anhydrase	Domain

• Tissue specificity: TISSUE SPECIFICITY: Expressed primarily in carcinoma cells lines. Expression is restricted to very few normal tissues and the most abundant expression is found in the epithelial cells of gastric mucosa.
• Sequence:

  MAPLCPSPWLPLLIPAPAPGLTVQLLLSLLLLVPVHPQRLPRMQEDSPLGGGSSGEDDPL
  GEEDLPSEEDSPREEDPPGEEDLPGEEDLPGEEDLPEVKPKSEEEGSLKLEDLPTVEAPG
  DPQEPQNNAHRDKEGDDQSHWRYGGDPPWPRVSPACAGRFQSPVDIRPQLAAFCPALRPL
  ELLGFQLPPLPELRLRNNGHSVQLTLPPGLEMALGPGREYRALQLHLHWGAAGRPGSEHT
  VEGHRFPAEIHVVHLSTAFARVDEALGRPGGLAVLAAFLEEGPEENSAYEQLLSRLEEIA
  EEGSETQVPGLDISALLPSDFSRYFQYEGSLTTPPCAQGVIWTVFNQTVMLSAKQLHTLS
  DTLWGPGDSRLQLNFRATQPLNGRVIEASFPAGVDSSPRAAEPVQLNSCLAAGDILALVF
  GLLFAVTSVAFLVQMRRQHRRGTKGGVSYRPAEVAETGA

• Sequence length: 459

Pathways

KEGG:

Nitrogen metabolism
Metabolic pathways

Reactome:

Regulation of gene expression by Hypoxia-inducible Factor
Reversible hydration of carbon dioxide