PEMT (phosphatidylethanolamine N-methyltransferase)

Gene

• Entrez ID: 10400
• Gene name: Phosphatidylethanolamine N-methyltransferase
• Gene symbol: PEMT
• Synonyms (NCBI Gene): PEAMT, PEMPT, PEMT2, PLMT, PNMT
• Chromosome: 17
• Chromosome location: 17p11.2
• Summary: Phosphatidylcholine (PC) is the most abundant mammalian phospholipid. This gene encodes an enzyme which converts phosphatidylethanolamine to phosphatidylcholine by sequential methylation in the liver. Another distinct synthetic pathway in nucleated cells

miRNA

miRTarBase ID	miRNA	Experiments	Reference
MIRT022625	hsa-miR-124-3p	Microarray	18668037
MIRT1224933	hsa-miR-1	CLIP-seq
MIRT1224934	hsa-miR-1257	CLIP-seq
MIRT1224935	hsa-miR-147	CLIP-seq
MIRT1224936	hsa-miR-206	CLIP-seq
MIRT1224937	hsa-miR-3911	CLIP-seq
MIRT1224938	hsa-miR-3940-5p	CLIP-seq
MIRT1224939	hsa-miR-4507	CLIP-seq
MIRT1224940	hsa-miR-4633-3p	CLIP-seq
MIRT1224941	hsa-miR-4790-3p	CLIP-seq
MIRT1224942	hsa-miR-613	CLIP-seq
MIRT1224943	hsa-miR-615-5p	CLIP-seq
MIRT1224944	hsa-miR-644	CLIP-seq
MIRT2687568	hsa-miR-1245b-5p	CLIP-seq
MIRT2687569	hsa-miR-1972	CLIP-seq
MIRT2687570	hsa-miR-3142	CLIP-seq
MIRT2687571	hsa-miR-3614-5p	CLIP-seq
MIRT2687572	hsa-miR-4252	CLIP-seq
MIRT2687573	hsa-miR-4260	CLIP-seq
MIRT2687574	hsa-miR-4435	CLIP-seq
MIRT2687575	hsa-miR-4459	CLIP-seq
MIRT2687576	hsa-miR-4650-5p	CLIP-seq
MIRT2687577	hsa-miR-4695-5p	CLIP-seq
MIRT2687578	hsa-miR-4755-3p	CLIP-seq
MIRT2687579	hsa-miR-485-5p	CLIP-seq
MIRT2687580	hsa-miR-486-3p	CLIP-seq
MIRT2687581	hsa-miR-665	CLIP-seq

Gene ontology (GO)

GO ID	Ontology	Definition	Evidence	Reference
GO:0000773	Function	Phosphatidyl-N-methylethanolamine N-methyltransferase activity	IEA
GO:0001835	Process	Blastocyst hatching	IEA
GO:0004608	Function	Phosphatidylethanolamine N-methyltransferase activity	IBA
GO:0004608	Function	Phosphatidylethanolamine N-methyltransferase activity	IEA
GO:0004608	Function	Phosphatidylethanolamine N-methyltransferase activity	TAS	9989271
GO:0005515	Function	Protein binding	IPI	32296183
GO:0005739	Component	Mitochondrion	IDA
GO:0005739	Component	Mitochondrion	IEA
GO:0005783	Component	Endoplasmic reticulum	IDA
GO:0005783	Component	Endoplasmic reticulum	IEA
GO:0005789	Component	Endoplasmic reticulum membrane	IEA
GO:0005789	Component	Endoplasmic reticulum membrane	TAS
GO:0005829	Component	Cytosol	IDA
GO:0006629	Process	Lipid metabolic process	IEA
GO:0006656	Process	Phosphatidylcholine biosynthetic process	IBA
GO:0006656	Process	Phosphatidylcholine biosynthetic process	IEA
GO:0006656	Process	Phosphatidylcholine biosynthetic process	TAS
GO:0006686	Process	Sphingomyelin biosynthetic process	IEA
GO:0008168	Function	Methyltransferase activity	IEA
GO:0008654	Process	Phospholipid biosynthetic process	IEA
GO:0008757	Function	S-adenosylmethionine-dependent methyltransferase activity	IEA
GO:0016020	Component	Membrane	IEA
GO:0016740	Function	Transferase activity	IEA
GO:0031966	Component	Mitochondrial membrane	IEA
GO:0032259	Process	Methylation	IEA
GO:0120162	Process	Positive regulation of cold-induced thermogenesis	IEA
GO:0120162	Process	Positive regulation of cold-induced thermogenesis	ISS	26113536

Other IDs

• MIM: 602391
• HGNC: 8830
• e!Ensembl: ENSG00000133027

Protein

• UniProt ID: Q9UBM1
• Protein name: Phosphatidylethanolamine N-methyltransferase (PEAMT) (PEMT) (EC 2.1.1.17) (EC 2.1.1.71) (PEMT2) (Phospholipid methyltransferase) (PLMT)
• Protein function: Catalyzes the three sequential steps of the methylation pathway for the biosynthesis of phosphatidylcholine, a critical and essential component for membrane structure (PubMed:12431977, PubMed:15927961). Uses S-adenosylmethionine (S-adenosyl-L-me
• Family and domains:

  Pfam

  Accession	ID	Position in sequence	Description	Type
  PF04191	PEMT	88 → 192	Phospholipid methyltransferase	Family

• Tissue specificity: TISSUE SPECIFICITY: Primarily expressed in liver (at protein level). {ECO:0000269|PubMed:12431977}.
• Sequence:

  MTRLLGYVDPLDPSFVAAVITITFNPLYWNVVARWEHKTRKLSRAFGSPYLACYSLSVTI
  LLLNFLRSHCFTQAMLSQPRMESLDTPAAYSLGLALLGLGVVLVLSSFFALGFAGTFLGD
  YFGILKEARVTVFPFNILDNPMYWGSTANYLGWAIMHASPTGLLLTVLVALTYIVALLYE
  EPFTAEIYRQKASGSHKRS

• Sequence length: 199

Pathways

KEGG:

Glycerophospholipid metabolism
Metabolic pathways

Reactome:

Synthesis of PC