DGKA (diacylglycerol kinase alpha)

Gene

• Entrez ID: 1606
• Gene name: Diacylglycerol kinase alpha
• Gene symbol: DGKA
• Synonyms (NCBI Gene): DAGK, DAGK1, DGK-alpha
• Chromosome: 12
• Chromosome location: 12q13.2
• Summary: The protein encoded by this gene belongs to the eukaryotic diacylglycerol kinase family. It acts as a modulator that competes with protein kinase C for the second messenger diacylglycerol in intracellular signaling pathways. It also plays an important rol

miRNA

miRTarBase ID	miRNA	Experiments	Reference
MIRT023096	hsa-miR-124-3p	Microarray	18668037
MIRT042155	hsa-miR-484	CLASH	23622248
MIRT933827	hsa-miR-1245b-3p	CLIP-seq
MIRT933828	hsa-miR-1827	CLIP-seq
MIRT933829	hsa-miR-3942-3p	CLIP-seq
MIRT933830	hsa-miR-4731-3p	CLIP-seq
MIRT933831	hsa-miR-4801	CLIP-seq
MIRT2211869	hsa-miR-1207-5p	CLIP-seq
MIRT2211870	hsa-miR-320e	CLIP-seq
MIRT2211871	hsa-miR-361-3p	CLIP-seq
MIRT2211872	hsa-miR-4433	CLIP-seq
MIRT2211873	hsa-miR-4722-5p	CLIP-seq
MIRT2211874	hsa-miR-4736	CLIP-seq
MIRT2211875	hsa-miR-4763-3p	CLIP-seq
MIRT2386862	hsa-miR-3688-5p	CLIP-seq
MIRT2386863	hsa-miR-3908	CLIP-seq
MIRT2386864	hsa-miR-4797-5p	CLIP-seq
MIRT2386865	hsa-miR-885-3p	CLIP-seq

Gene ontology (GO)

GO ID	Ontology	Definition	Evidence	Reference
GO:0000166	Function	Nucleotide binding	IEA
GO:0004143	Function	ATP-dependent diacylglycerol kinase activity	IBA
GO:0004143	Function	ATP-dependent diacylglycerol kinase activity	IDA	2175712, 15544348, 18004883, 22627129
GO:0004143	Function	ATP-dependent diacylglycerol kinase activity	IEA
GO:0004143	Function	ATP-dependent diacylglycerol kinase activity	TAS
GO:0005509	Function	Calcium ion binding	IEA
GO:0005524	Function	ATP binding	IEA
GO:0005543	Function	Phospholipid binding	IEA
GO:0005737	Component	Cytoplasm	IEA
GO:0005829	Component	Cytosol	IDA	2175712
GO:0005829	Component	Cytosol	IEA
GO:0005886	Component	Plasma membrane	IBA
GO:0005886	Component	Plasma membrane	IEA
GO:0005886	Component	Plasma membrane	TAS
GO:0006629	Process	Lipid metabolic process	IEA
GO:0006654	Process	Phosphatidic acid biosynthetic process	IBA
GO:0006654	Process	Phosphatidic acid biosynthetic process	IDA	2175712, 15544348, 22627129
GO:0006654	Process	Phosphatidic acid biosynthetic process	IEA
GO:0007165	Process	Signal transduction	IEA
GO:0007200	Process	Phospholipase C-activating G protein-coupled receptor signaling pathway	IEA
GO:0008270	Function	Zinc ion binding	IEA
GO:0008289	Function	Lipid binding	IDA	22627129
GO:0008289	Function	Lipid binding	IEA
GO:0016020	Component	Membrane	HDA	19946888
GO:0016301	Function	Kinase activity	IDA	18004883
GO:0016301	Function	Kinase activity	IEA
GO:0016740	Function	Transferase activity	IEA
GO:0030168	Process	Platelet activation	IEA
GO:0030168	Process	Platelet activation	TAS
GO:0035556	Process	Intracellular signal transduction	IBA
GO:0035556	Process	Intracellular signal transduction	TAS	2175712
GO:0046339	Process	Diacylglycerol metabolic process	IBA
GO:0046339	Process	Diacylglycerol metabolic process	IDA	2175712, 15544348, 22627129
GO:0046339	Process	Diacylglycerol metabolic process	IEA
GO:0046486	Process	Glycerolipid metabolic process	IDA	22627129
GO:0046486	Process	Glycerolipid metabolic process	IEA
GO:0046834	Process	Lipid phosphorylation	IDA	2175712, 15544348, 18004883, 22627129
GO:0046834	Process	Lipid phosphorylation	IEA
GO:0046872	Function	Metal ion binding	IEA
GO:0047649	Function	Alkylglycerol kinase activity	IEA

Other IDs

• MIM: 125855
• HGNC: 2849
• e!Ensembl: ENSG00000065357

Protein

• UniProt ID: P23743
• Protein name: Diacylglycerol kinase alpha (DAG kinase alpha) (EC 2.7.1.107) (EC 2.7.1.93) (80 kDa diacylglycerol kinase) (Diglyceride kinase alpha) (DGK-alpha)
• Protein function: Diacylglycerol kinase that converts diacylglycerol/DAG into phosphatidic acid/phosphatidate/PA and regulates the respective levels of these two bioactive lipids (PubMed:15544348, PubMed:2175712). Thereby, acts as a central switch between the sig
• PDB: 1TUZ, 6IIE
• Family and domains:

  Pfam

  Accession	ID	Position in sequence	Description	Type
  PF14513	DAG_kinase_N	4 → 88	Diacylglycerol kinase N-terminus	Domain
  PF14513	DAG_kinase_N	82 → 110	Diacylglycerol kinase N-terminus	Domain
  PF00130	C1_1	206 → 257	Phorbol esters/diacylglycerol binding domain (C1 domain)	Domain
  PF00130	C1_1	270 → 322	Phorbol esters/diacylglycerol binding domain (C1 domain)	Domain
  PF00781	DAGK_cat	376 → 497	Diacylglycerol kinase catalytic domain	Family
  PF00609	DAGK_acc	520 → 701	Diacylglycerol kinase accessory domain	Family

• Tissue specificity: TISSUE SPECIFICITY: Expressed in lymphocytes. {ECO:0000269|PubMed:2175712}.
• Sequence:

  MAKERGLISPSDFAQLQKYMEYSTKKVSDVLKLFEDGEMAKYVQGDAIGYEGFQQFLKIY
  LEVDNVPRHLSLALFQSFETGHCLNETNVTKDVVCLNDVSCYFSLLEGGRPEDKLEFTFK
  LYDTDRNGILDSSEVDKIILQMMRVAEYLDWDVSELRPILQEMMKEIDYDGSGSVSQAEW
  VRAGATTVPLLVLLGLEMTLKDDGQHMWRPKRFPRPVYCNLCESSIGLGKQGLSCNLCKY
  TVHDQCAMKALPCEVSTYAKSRKDIGVQSHVWVRGGCESGRCDRCQKKIRIYHSLTGLHC
  VWCHLEIHDDCLQAVGHECDCGLLRDHILPPSSIYPSVLASGPDRKNSKTSQKTMDDLNL
  STSEALRIDPVPNTHPLLVFVNPKSGGKQGQRVLWKFQYILNPRQVFNLLKDGPEIGLRL
  FKDVPDSRILVCGGDGTVGWILETIDKANLPVLPPVAVLPLGTGNDLARCLRWGGGYEGQ
  NLAKILKDLEMSKVVHMDRWSVEVIPQQTEEKSDPVPFQIINNYFSIGVDASIAHRFHIM
  REKYPEKFNSRMKNKLWYFEFATSESIFSTCKKLEESLTVEICGKPLDLSNLSLEGIAVL
  NIPSMHGGSNLWGDTRRPHGDIYGINQALGATAKVITDPDILKTCVPDLSDKRLEVVGLE
  GAIEMGQIYTKLKNAGRRLAKCSEITFHTTKTLPMQIDGEPWMQTPCTIKITHKNQMPML
  MGPPPRSTNFFGFLS

• Sequence length: 735

Pathways

KEGG:

Glycerolipid metabolism
Glycerophospholipid metabolism
Metabolic pathways
Phosphatidylinositol signaling system
Phospholipase D signaling pathway
Choline metabolism in cancer

Reactome:

Effects of PIP2 hydrolysis

Associated diseases

Evidence Score:

• ★☆☆☆☆  Gene-disease association found in Text Mining only
• ★★☆☆☆  Found in Text Mining and Unknown/Other Associations
• ★★★☆☆  Reported in Unknown/Other Associations across ≥2 Sources
• ★★★★☆  ClinVar: Pathogenic/Likely Pathogenic (<5 Variants)
• ★★★★★  ClinVar: Pathogenic/Likely Pathogenic (≥5 Variants)

Unknown / Other Associations
Phenotype Name	Clinical Significance	Source	Evidence Score
Acute myeloid leukemia	Benign	ClinVar	★★☆☆☆ Found in Text Mining + Unknown/Other Associations
Colon adenocarcinoma	Benign	ClinVar	★★☆☆☆ Found in Text Mining + Unknown/Other Associations
DGKA-related disorder	Benign; Likely benign	ClinVar	★★☆☆☆ Found in Text Mining + Unknown/Other Associations
Gastric cancer	Benign	ClinVar	★★☆☆☆ Found in Text Mining + Unknown/Other Associations
Hepatocellular carcinoma	Benign	ClinVar	★★☆☆☆ Found in Text Mining + Unknown/Other Associations
HYPEROPIA	—	GWAS catalog	★★☆☆☆ Found in Text Mining + Unknown/Other Associations
Malignant tumor of esophagus	Benign	ClinVar	★★☆☆☆ Found in Text Mining + Unknown/Other Associations
Nonpapillary renal cell carcinoma	Benign	ClinVar	★★☆☆☆ Found in Text Mining + Unknown/Other Associations
Ovarian serous cystadenocarcinoma	Benign	ClinVar	★★☆☆☆ Found in Text Mining + Unknown/Other Associations
Sarcoma	Benign	ClinVar	★★☆☆☆ Found in Text Mining + Unknown/Other Associations
Thymoma	Benign	ClinVar	★★☆☆☆ Found in Text Mining + Unknown/Other Associations
Thyroid cancer, nonmedullary, 1	Benign	ClinVar	★★☆☆☆ Found in Text Mining + Unknown/Other Associations

Associations from Text Mining
Disease Name	Disease (Merged)	Source	PMID	Relationship Type	Evidence Score
Adenocarcinoma of lung (disorder)	Lung adenocarcinoma	BEFREE	15952876		★☆☆☆☆ Found in Text Mining only
Aggressive Periodontitis	Aggressive Periodontitis	LHGDN	14734770		★☆☆☆☆ Found in Text Mining only
Breast Carcinoma	Breast Carcinoma	BEFREE	25339152		★☆☆☆☆ Found in Text Mining only
Depressive Disorder	Major depressive disorder	Pubtator	34341332	Associate	★☆☆☆☆ Found in Text Mining only
Diabetic Nephropathy	Diabetic Nephropathy	BEFREE	28572624, 29297254, 30715374		★☆☆☆☆ Found in Text Mining only
Endometrial Carcinoma	Endometrial carcinoma	BEFREE	21802511		★☆☆☆☆ Found in Text Mining only
Glioblastoma	Glioblastoma	BEFREE	23558954, 24158111, 29048560		★☆☆☆☆ Found in Text Mining only
Glioblastoma	Glioblastoma	Pubtator	23558954	Associate	★☆☆☆☆ Found in Text Mining only
Glioblastoma Multiforme	Glioblastoma	BEFREE	23558954, 24158111, 29048560		★☆☆☆☆ Found in Text Mining only
leukemia	Leukemia	BEFREE	24171497		★☆☆☆☆ Found in Text Mining only
Leukemia Myeloid Acute	Myeloid leukemia	Pubtator	29688850	Associate	★☆☆☆☆ Found in Text Mining only
Liver carcinoma	Liver carcinoma	BEFREE	22425622, 30536880		★☆☆☆☆ Found in Text Mining only
Lung Neoplasms	Lung neoplasms	Pubtator	30158316	Associate	★☆☆☆☆ Found in Text Mining only
Lymphoproliferative Disorders	Lymphoproliferative syndrome	Pubtator	22048771, 30611057	Associate	★☆☆☆☆ Found in Text Mining only
Malignant neoplasm of breast	Breast Cancer	BEFREE	25339152		★☆☆☆☆ Found in Text Mining only
Malignant neoplasm of endometrium	Endometrial Cancer	BEFREE	21802511		★☆☆☆☆ Found in Text Mining only
Malignant Neoplasms	Malignant Neoplasm	BEFREE	23558954, 23847350, 25339152, 26420856, 28316970, 29572701, 30498210, 30511409, 30532074		★☆☆☆☆ Found in Text Mining only
melanoma	Melanoma	LHGDN	17276726		★☆☆☆☆ Found in Text Mining only
melanoma	Melanoma	BEFREE	23558954, 30536880		★☆☆☆☆ Found in Text Mining only
Melanoma	Melanoma	Pubtator	23558954	Associate	★☆☆☆☆ Found in Text Mining only
Neoplasms	Neoplasms	BEFREE	22425622, 23847350, 25339152, 29572701		★☆☆☆☆ Found in Text Mining only
Pancreatic carcinoma	Pancreatic carcinoma	BEFREE	29048560		★☆☆☆☆ Found in Text Mining only
Pancreatic Neoplasms	Pancreatic neoplasm	Pubtator	20581473, 35030986	Associate	★☆☆☆☆ Found in Text Mining only
Respiration Disorders	Respiration Disorders	BEFREE	31766109		★☆☆☆☆ Found in Text Mining only
Squamous cell carcinoma	Carcinoma	BEFREE	30532074		★☆☆☆☆ Found in Text Mining only
Squamous cell carcinoma of esophagus	Esophagus Neoplasm	BEFREE	30532074		★☆☆☆☆ Found in Text Mining only
Stomach Neoplasms	Stomach neoplasms	Pubtator	27498782	Inhibit	★☆☆☆☆ Found in Text Mining only