NUAK1 (NUAK family kinase 1)

Gene

• Entrez ID: 9891
• Gene name: NUAK family kinase 1
• Gene symbol: NUAK1
• Synonyms: ARK5
• Chromosome: 12
• Chromosome location: 12q23.3

miRNA

miRTarBase ID	miRNA	Experiments	Reference
MIRT021136	hsa-miR-186-5p	Sequencing	20371350
MIRT023849	hsa-miR-1-3p	Microarray	18668037
MIRT028149	hsa-miR-93-5p	Sequencing	20371350
MIRT050078	hsa-miR-26a-5p	CLASH	23622248
MIRT438079	hsa-miR-211-5p	Luciferase reporter assay, Western blot, qRT-PCR	23934065
MIRT438079	hsa-miR-211-5p	Luciferase reporter assay, Western blot, qRT-PCR	23934065
MIRT438079	hsa-miR-211-5p	Luciferase reporter assay, Western blot, qRT-PCR	23934065
MIRT438079	hsa-miR-211-5p	Luciferase reporter assay, Western blot, qRT-PCR	23934065
MIRT438079	hsa-miR-211-5p	Luciferase reporter assay, Western blot, qRT-PCR	23934065
MIRT438079	hsa-miR-211-5p	Luciferase reporter assay, Western blot, qRT-PCR	23934065
MIRT438079	hsa-miR-211-5p	Luciferase reporter assay, Western blot, qRT-PCR	23934065
MIRT438079	hsa-miR-211-5p	Luciferase reporter assay, Western blot, qRT-PCR	23934065
MIRT655709	hsa-miR-556-3p	HITS-CLIP	23824327
MIRT440086	hsa-miR-770-5p	HITS-CLIP	24374217
MIRT440086	hsa-miR-770-5p	HITS-CLIP	24374217
MIRT733136	hsa-miR-30b-5p	Luciferase reporter assay, qRT-PCR, Western blotting	31949655
MIRT655709	hsa-miR-556-3p	HITS-CLIP	23824327
MIRT756449	hsa-miR-424-5p	Luciferase reporter assay	34097192
MIRT1196381	hsa-miR-103a	CLIP-seq
MIRT1196382	hsa-miR-105	CLIP-seq
MIRT1196383	hsa-miR-107	CLIP-seq
MIRT1196384	hsa-miR-1200	CLIP-seq
MIRT1196385	hsa-miR-1238	CLIP-seq
MIRT1196386	hsa-miR-126	CLIP-seq
MIRT1196387	hsa-miR-127-5p	CLIP-seq
MIRT1196388	hsa-miR-1290	CLIP-seq
MIRT739840	hsa-miR-1470	CLIP-seq
MIRT1196389	hsa-miR-15a	CLIP-seq
MIRT1196390	hsa-miR-15b	CLIP-seq
MIRT1196391	hsa-miR-16	CLIP-seq
MIRT1196392	hsa-miR-182	CLIP-seq
MIRT1196393	hsa-miR-193a-5p	CLIP-seq
MIRT1196394	hsa-miR-195	CLIP-seq
MIRT1196395	hsa-miR-196a	CLIP-seq
MIRT1196396	hsa-miR-196b	CLIP-seq
MIRT1196397	hsa-miR-1973	CLIP-seq
MIRT1196398	hsa-miR-214	CLIP-seq
MIRT1196399	hsa-miR-22	CLIP-seq
MIRT1196400	hsa-miR-224	CLIP-seq
MIRT1196401	hsa-miR-2355-5p	CLIP-seq
MIRT1196402	hsa-miR-28-5p	CLIP-seq
MIRT1196403	hsa-miR-3124-3p	CLIP-seq
MIRT1196404	hsa-miR-3127-3p	CLIP-seq
MIRT1196405	hsa-miR-3139	CLIP-seq
MIRT1196406	hsa-miR-3140-5p	CLIP-seq
MIRT1196407	hsa-miR-3148	CLIP-seq
MIRT1196408	hsa-miR-3167	CLIP-seq
MIRT1196409	hsa-miR-3173-3p	CLIP-seq
MIRT1196410	hsa-miR-3614-5p	CLIP-seq
MIRT1196411	hsa-miR-3619-5p	CLIP-seq
MIRT1196412	hsa-miR-3667-3p	CLIP-seq
MIRT1196413	hsa-miR-3689d	CLIP-seq
MIRT1196414	hsa-miR-3691-5p	CLIP-seq
MIRT1196415	hsa-miR-3923	CLIP-seq
MIRT1196416	hsa-miR-3927	CLIP-seq
MIRT1196417	hsa-miR-3929	CLIP-seq
MIRT1196418	hsa-miR-424	CLIP-seq
MIRT1196419	hsa-miR-4419b	CLIP-seq
MIRT1196420	hsa-miR-4436b-3p	CLIP-seq
MIRT1196421	hsa-miR-4457	CLIP-seq
MIRT1196422	hsa-miR-4462	CLIP-seq
MIRT1196423	hsa-miR-4469	CLIP-seq
MIRT1196424	hsa-miR-4470	CLIP-seq
MIRT1196425	hsa-miR-4478	CLIP-seq
MIRT1196426	hsa-miR-4491	CLIP-seq
MIRT1196427	hsa-miR-4530	CLIP-seq
MIRT1196428	hsa-miR-4635	CLIP-seq
MIRT1196429	hsa-miR-4640-3p	CLIP-seq
MIRT1196430	hsa-miR-4640-5p	CLIP-seq
MIRT1196431	hsa-miR-4655-3p	CLIP-seq
MIRT1196432	hsa-miR-4657	CLIP-seq
MIRT739839	hsa-miR-4667-3p	CLIP-seq
MIRT1196433	hsa-miR-4687-3p	CLIP-seq
MIRT739837	hsa-miR-4687-5p	CLIP-seq
MIRT1196434	hsa-miR-4691-5p	CLIP-seq
MIRT1196435	hsa-miR-4705	CLIP-seq
MIRT1196436	hsa-miR-4726-5p	CLIP-seq
MIRT1196437	hsa-miR-4752	CLIP-seq
MIRT739841	hsa-miR-4760-3p	CLIP-seq
MIRT1196438	hsa-miR-4765	CLIP-seq
MIRT1196439	hsa-miR-483-3p	CLIP-seq
MIRT1196440	hsa-miR-488	CLIP-seq
MIRT1196441	hsa-miR-497	CLIP-seq
MIRT1196442	hsa-miR-545	CLIP-seq
MIRT1196443	hsa-miR-548aa	CLIP-seq
MIRT1196444	hsa-miR-548n	CLIP-seq
MIRT1196445	hsa-miR-548t	CLIP-seq
MIRT1196446	hsa-miR-548u	CLIP-seq
MIRT1196447	hsa-miR-570	CLIP-seq
MIRT1196448	hsa-miR-583	CLIP-seq
MIRT1196449	hsa-miR-603	CLIP-seq
MIRT1196450	hsa-miR-616	CLIP-seq
MIRT1196451	hsa-miR-708	CLIP-seq
MIRT1196452	hsa-miR-761	CLIP-seq
MIRT739838	hsa-miR-766	CLIP-seq
MIRT1196453	hsa-miR-767-3p	CLIP-seq
MIRT1196454	hsa-miR-876-5p	CLIP-seq
MIRT1196381	hsa-miR-103a	CLIP-seq
MIRT1196383	hsa-miR-107	CLIP-seq
MIRT2056983	hsa-miR-145	CLIP-seq
MIRT1196401	hsa-miR-2355-5p	CLIP-seq
MIRT1196412	hsa-miR-3667-3p	CLIP-seq
MIRT1196413	hsa-miR-3689d	CLIP-seq
MIRT1196415	hsa-miR-3923	CLIP-seq
MIRT2056984	hsa-miR-4287	CLIP-seq
MIRT2056985	hsa-miR-4299	CLIP-seq
MIRT2056986	hsa-miR-4312	CLIP-seq
MIRT1196423	hsa-miR-4469	CLIP-seq
MIRT2056987	hsa-miR-4685-3p	CLIP-seq
MIRT1196435	hsa-miR-4705	CLIP-seq
MIRT2056988	hsa-miR-4708-3p	CLIP-seq
MIRT1196439	hsa-miR-483-3p	CLIP-seq
MIRT1196448	hsa-miR-583	CLIP-seq
MIRT2056989	hsa-miR-668	CLIP-seq
MIRT2056990	hsa-miR-873	CLIP-seq
MIRT1196381	hsa-miR-103a	CLIP-seq
MIRT1196383	hsa-miR-107	CLIP-seq
MIRT2056983	hsa-miR-145	CLIP-seq
MIRT1196389	hsa-miR-15a	CLIP-seq
MIRT1196390	hsa-miR-15b	CLIP-seq
MIRT1196391	hsa-miR-16	CLIP-seq
MIRT1196394	hsa-miR-195	CLIP-seq
MIRT1196398	hsa-miR-214	CLIP-seq
MIRT2285412	hsa-miR-3065-3p	CLIP-seq
MIRT2285413	hsa-miR-3185	CLIP-seq
MIRT2285414	hsa-miR-3187-3p	CLIP-seq
MIRT1196411	hsa-miR-3619-5p	CLIP-seq
MIRT1196417	hsa-miR-3929	CLIP-seq
MIRT1196418	hsa-miR-424	CLIP-seq
MIRT1196419	hsa-miR-4419b	CLIP-seq
MIRT1196420	hsa-miR-4436b-3p	CLIP-seq
MIRT1196425	hsa-miR-4478	CLIP-seq
MIRT1196427	hsa-miR-4530	CLIP-seq
MIRT1196428	hsa-miR-4635	CLIP-seq
MIRT1196430	hsa-miR-4640-5p	CLIP-seq
MIRT1196433	hsa-miR-4687-3p	CLIP-seq
MIRT1196436	hsa-miR-4726-5p	CLIP-seq
MIRT1196441	hsa-miR-497	CLIP-seq
MIRT1196452	hsa-miR-761	CLIP-seq
MIRT2391792	hsa-miR-339-5p	CLIP-seq
MIRT1196412	hsa-miR-3667-3p	CLIP-seq
MIRT2056984	hsa-miR-4287	CLIP-seq
MIRT2056985	hsa-miR-4299	CLIP-seq
MIRT1196423	hsa-miR-4469	CLIP-seq
MIRT2056987	hsa-miR-4685-3p	CLIP-seq
MIRT2391793	hsa-miR-4713-5p	CLIP-seq
MIRT2056989	hsa-miR-668	CLIP-seq
MIRT2056985	hsa-miR-4299	CLIP-seq
MIRT2056989	hsa-miR-668	CLIP-seq
MIRT2686668	hsa-miR-3157-5p	CLIP-seq
MIRT2056985	hsa-miR-4299	CLIP-seq
MIRT2686669	hsa-miR-4691-3p	CLIP-seq
MIRT2056989	hsa-miR-668	CLIP-seq

Gene ontology (GO)

GO ID	Ontology	Definition	Evidence	Reference
GO:0001650	Component	Fibrillar center	IDA
GO:0002039	Function	P53 binding	IPI	21317932
GO:0004674	Function	Protein serine/threonine kinase activity	IBA	21873635
GO:0004674	Function	Protein serine/threonine kinase activity	IDA	19927127, 20354225, 21317932
GO:0004674	Function	Protein serine/threonine kinase activity	TAS
GO:0005515	Function	Protein binding	IPI	19927127, 20354225, 21317932, 25329316
GO:0005524	Function	ATP binding	IEA
GO:0005634	Component	Nucleus	IBA	21873635
GO:0005634	Component	Nucleus	IDA	21317932
GO:0005654	Component	Nucleoplasm	IDA
GO:0005654	Component	Nucleoplasm	TAS
GO:0005737	Component	Cytoplasm	IBA	21873635
GO:0005737	Component	Cytoplasm	IDA	21317932
GO:0006468	Process	Protein phosphorylation	IBA	21873635
GO:0006468	Process	Protein phosphorylation	IDA	25329316
GO:0006974	Process	Cellular response to DNA damage stimulus	IEA
GO:0007155	Process	Cell adhesion	IEA
GO:0015630	Component	Microtubule cytoskeleton	IDA
GO:0030155	Process	Regulation of cell adhesion	IDA	20354225
GO:0035507	Process	Regulation of myosin-light-chain-phosphatase activity	IDA	20354225
GO:0035556	Process	Intracellular signal transduction	IBA	21873635
GO:0042127	Process	Regulation of cell population proliferation	TAS	21317932
GO:0042149	Process	Cellular response to glucose starvation	IBA	21873635
GO:0046872	Function	Metal ion binding	IEA
GO:0106310	Function	Protein serine kinase activity	IEA
GO:0106311	Function	Protein threonine kinase activity	IEA
GO:1901796	Process	Regulation of signal transduction by p53 class mediator	TAS
GO:2000772	Process	Regulation of cellular senescence	IDA	19927127

Other IDs

• MIM: 608130
• HGNC: 14311
• e!Ensembl: ENSG00000074590

Protein

• UniProt ID: O60285
• Protein name: NUAK family SNF1-like kinase 1 (EC 2.7.11.1) (AMPK-related protein kinase 5) (ARK5) (Omphalocele kinase 1)
• Protein function: Serine/threonine-protein kinase involved in various processes such as cell adhesion, regulation of cell ploidy and senescence, cell proliferation and tumor progression. Phosphorylates ATM, CASP6, LATS1, PPP1R12A and p53/TP53. Acts as a regulator
• Family and domains:

  Pfam

  Accession	ID	Position in sequence	Description	Type
  PF00069	Pkinase	55 → 306	Protein kinase domain	Domain

• Sequence:

  MEGAAAPVAGDRPDLGLGAPGSPREAVAGATAALEPRKPHGVKRHHHKHNLKHRYELQET
  LGKGTYGKVKRATERFSGRVVAIKSIRKDKIKDEQDMVHIRREIEIMSSLNHPHIISIYE
  VFENKDKIVIIMEYASKGELYDYISERRRLSERETRHFFRQIVSAVHYCHKNGVVHRDLK
  LENILLDDNCNIKIADFGLSNLYQKDKFLQTFCGSPLYASPEIVNGRPYRGPEVDSWALG
  VLLYTLVYGTMPFDGFDHKNLIRQISSGEYREPTQPSDARGLIRWMLMVNPDRRATIEDI
  ANHWWVNWGYKSSVCDCDALHDSESPLLARIIDWHHRSTGLQADTEAKMKGLAKPTTSEV
  MLERQRSLKKSKKENDFAQSGQDAVPESPSKLSSKRPKGILKKRSNSEHRSHSTGFIEGV
  VGPALPSTFKMEQDLCRTGVLLPSSPEAEVPGKLSPKQSATMPKKGILKKTQQRESGYYS
  SPERSESSELLDSNDVMGSSIPSPSPPDPARVTSHSLSCRRKGILKHSSKYSAGTMDPAL
  VSPEMPTLESLSEPGVPAEGLSRSYSRPSSVISDDSVLSSDSFDLLDLQENRPARQRIRS
  CVSAENFLQIQDFEGLQNRPRPQYLKRYRNRLADSSFSLLTDMDDVTQVYKQALEICSKL
  N

• Sequence length: 661

Pathways

Reactome:

Regulation of TP53 Activity through Phosphorylation

Associated diseases

Causal
Disease name	Disease term	dbSNP ID	References
Attention deficit hyperactivity disorder	Attention deficit hyperactivity disorder	rs120074176, rs786205019	26174813
Multiple myeloma	Multiple Myeloma	rs11540652, rs78311289, rs121913482, rs397507340, rs121913343, rs121913240, rs121913529, rs730882018, rs1057517992, rs121913527, rs756183569, rs746646631, rs1574706907, rs372078034, rs745380962, rs751524927, rs1575079076, rs1575446356, rs1478603808, rs1578264146, rs1578673280, rs1579484570, rs1579491104, rs1171390403, rs1582867955, rs764264135, rs951047896, rs890521687, rs1581495906, rs1587941402, rs1003155450, rs1588299621, rs1591100766, rs1591693095, rs1029296641, rs1593107841, rs1208575764, rs1593835248, rs1594406727, rs1594966387, rs1595889508, rs1159294530, rs1597073318, rs1135402871, rs1599413207, rs1418268495, rs1212577459, rs1600394490, rs1455074519, rs1603113792, rs1603415028, rs1602247047, rs1603452612	26873845