POLB (DNA polymerase beta)

Gene

• Entrez ID: 5423
• Gene name: DNA polymerase beta
• Gene symbol: POLB
• Synonyms: -
• Chromosome: 8
• Chromosome location: 8p11.21
• Summary: The protein encoded by this gene is a DNA polymerase involved in base excision and repair, also called gap-filling DNA synthesis. The encoded protein, acting as a monomer, is normally found in the cytoplasm, but it translocates to the nucleus upon DNA dam

miRNA

miRTarBase ID	miRNA	Experiments	Reference
MIRT051150	hsa-miR-16-5p	CLASH	23622248
MIRT035975	hsa-miR-1301-3p	CLASH	23622248

Transcription factors

Transcription factor	Regulation	Reference
ATF2	Activation	10518804

Gene ontology (GO)

GO ID	Ontology	Definition	Evidence	Reference
GO:0001701	Process	In utero embryonic development	IEA
GO:0003684	Function	Damaged DNA binding	IEA
GO:0003887	Function	DNA-directed DNA polymerase activity	IBA	21873635
GO:0003887	Function	DNA-directed DNA polymerase activity	IDA	9207062, 16600869
GO:0003887	Function	DNA-directed DNA polymerase activity	TAS
GO:0003906	Function	DNA-(apurinic or apyrimidinic site) endonuclease activity	TAS
GO:0005515	Function	Protein binding	IPI	9207062, 15520167, 25260657, 25416956, 25910212, 26496610, 28514442, 31515488, 32814053
GO:0005634	Component	Nucleus	IBA	21873635
GO:0005634	Component	Nucleus	IDA	19713937, 21362556
GO:0005654	Component	Nucleoplasm	TAS
GO:0005737	Component	Cytoplasm	IDA	15725623, 19713937, 21362556
GO:0005874	Component	Microtubule	IDA	15725623
GO:0005876	Component	Spindle microtubule	IDA	15725623
GO:0006261	Process	DNA-dependent DNA replication	TAS	8168825
GO:0006281	Process	DNA repair	TAS	8168825
GO:0006284	Process	Base-excision repair	IBA	21873635
GO:0006284	Process	Base-excision repair	IDA	16600869
GO:0006284	Process	Base-excision repair	IMP	21362556
GO:0006286	Process	Base-excision repair, base-free sugar-phosphate removal	TAS
GO:0006287	Process	Base-excision repair, gap-filling	IEA
GO:0006288	Process	Base-excision repair, DNA ligation	TAS
GO:0006290	Process	Pyrimidine dimer repair	IEA
GO:0006297	Process	Nucleotide-excision repair, DNA gap filling	IMP	20227374
GO:0006303	Process	Double-strand break repair via nonhomologous end joining	IBA	21873635
GO:0006954	Process	Inflammatory response	IEA
GO:0006974	Process	Cellular response to DNA damage stimulus	IMP	21362556
GO:0007435	Process	Salivary gland morphogenesis	IEA
GO:0007568	Process	Aging	IEA
GO:0008017	Function	Microtubule binding	IDA	15725623
GO:0008630	Process	Intrinsic apoptotic signaling pathway in response to DNA damage	IEA
GO:0010332	Process	Response to gamma radiation	IEA
GO:0016446	Process	Somatic hypermutation of immunoglobulin genes	IEA
GO:0016579	Process	Protein deubiquitination	TAS
GO:0016829	Function	Lyase activity	IDA	16600869
GO:0019899	Function	Enzyme binding	IPI	16600869, 19713937, 21362556
GO:0032991	Component	Protein-containing complex	IDA	25378300
GO:0045471	Process	Response to ethanol	IEA
GO:0046872	Function	Metal ion binding	IEA
GO:0048535	Process	Lymph node development	IEA
GO:0048536	Process	Spleen development	IEA
GO:0048872	Process	Homeostasis of number of cells	IEA
GO:0051402	Process	Neuron apoptotic process	IEA
GO:0055093	Process	Response to hyperoxia	IEA
GO:0071707	Process	Immunoglobulin heavy chain V-D-J recombination	IEA
GO:0071897	Process	DNA biosynthetic process	IEA

Other IDs

• MIM: 174760
• HGNC: 9174
• e!Ensembl: ENSG00000070501

Protein

• UniProt ID: P06746
• Protein name: DNA polymerase beta (EC 2.7.7.7) (5'-deoxyribose-phosphate lyase) (5'-dRP lyase) (EC 4.2.99.-) (AP lyase) (EC 4.2.99.18)
• Protein function: Repair polymerase that plays a key role in base-excision repair (PubMed:10556592, PubMed:9207062, PubMed:9572863). During this process, the damaged base is excised by specific DNA glycosylases, the DNA backbone is nicked at the abasic site by an
• PDB: 1BPX, 1BPY, 1BPZ, 1MQ2, 1MQ3, 1TV9, 1TVA, 1ZJM, 1ZJN, 1ZQA, 1ZQB, 1ZQC, 1ZQD, 1ZQE, 1ZQF, 1ZQG, 1ZQH, 1ZQI, 1ZQJ, 1ZQK, 1ZQL, 1ZQM, 1ZQN, 1ZQO, 1ZQP, 1ZQQ, 1ZQR, 1ZQS, 1ZQT, 2FMP, 2FMQ, 2FMS, 2I9G, 2ISO, 2ISP, 2P66, 2PXI, 3C2K, 3C2L, 3C2M, 3GDX, 3ISB, 3ISC, 3ISD, 3JPN, 3JPO, 3JPP, 3JPQ, 3JPR, 3JPS, 3JPT
• Family and domains:

  Pfam

  Accession	ID	Position in sequence	Description	Type
  PF14716	HHH_8	12 → 78	Helix-hairpin-helix domain	Domain
  PF10391	DNA_pol_lambd_f	98 → 147	Fingers domain of DNA polymerase lambda	Domain
  PF14792	DNA_pol_B_palm	149 → 262	DNA polymerase beta palm	Domain
  PF14791	DNA_pol_B_thumb	268 → 334	DNA polymerase beta thumb	Family

• Sequence:

  MSKRKAPQETLNGGITDMLTELANFEKNVSQAIHKYNAYRKAASVIAKYPHKIKSGAEAK
  KLPGVGTKIAEKIDEFLATGKLRKLEKIRQDDTSSSINFLTRVSGIGPSAARKFVDEGIK
  TLEDLRKNEDKLNHHQRIGLKYFGDFEKRIPREEMLQMQDIVLNEVKKVDSEYIATVCGS
  FRRGAESSGDMDVLLTHPSFTSESTKQPKLLHQVVEQLQKVHFITDTLSKGETKFMGVCQ
  LPSKNDEKEYPHRRIDIRLIPKDQYYCGVLYFTGSDIFNKNMRAHALEKGFTINEYTIRP
  LGVTGVAGEPLPVDSEKDIFDYIQWKYREPKDRSE

• Sequence length: 335

Pathways

KEGG:

Base excision repair
Human T-cell leukemia virus 1 infection
Viral carcinogenesis

Reactome:

POLB-Dependent Long Patch Base Excision Repair
Resolution of AP sites via the multiple-nucleotide patch replacement pathway
Resolution of AP sites via the single-nucleotide replacement pathway
APEX1-Independent Resolution of AP Sites via the Single Nucleotide Replacement Pathway
PCNA-Dependent Long Patch Base Excision Repair
Ub-specific processing proteases
Abasic sugar-phosphate removal via the single-nucleotide replacement pathway

Associated diseases

Causal
Disease name	Disease term	dbSNP ID	References
Colonic neoplasms	Malignant tumor of colon, Colonic Neoplasms	rs267607789, rs774277300, rs781222233, rs1060502734, rs1060503333, rs1339238483	25030372

Unknown
Disease name	Disease term	dbSNP ID	References
Urinary bladder cancer	Malignant neoplasm of urinary bladder		8631603
Bladder neoplasm	Bladder Neoplasm		8631603
Lymphocytic leukemia	Chronic Lymphocytic Leukemia		18006695