PFKFB4 (6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4)

Gene

• Entrez ID: 5210
• Gene name: 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4
• Gene symbol: PFKFB4
• Synonyms: -
• Chromosome: 3
• Chromosome location: 3p21.31
• Summary: The protein encoded by this gene is one of four bifunctional kinase/phosphatases that regulate the concentration of the glycolytic byproduct fructose-2,6-bisphosphate (F2,6BP). The encoded protein is highly expressed in cancer cells and is induced by hypo

miRNA

miRTarBase ID	miRNA	Experiments	Reference
MIRT016861	hsa-miR-335-5p	Microarray	18185580
MIRT040714	hsa-miR-92b-3p	CLASH	23622248
MIRT1226376	hsa-miR-214	CLIP-seq
MIRT1226377	hsa-miR-23a	CLIP-seq
MIRT1226378	hsa-miR-23b	CLIP-seq
MIRT1226379	hsa-miR-23c	CLIP-seq
MIRT1226380	hsa-miR-2467-3p	CLIP-seq
MIRT1226381	hsa-miR-296-3p	CLIP-seq
MIRT1226382	hsa-miR-3124-3p	CLIP-seq
MIRT1226383	hsa-miR-3138	CLIP-seq
MIRT1226384	hsa-miR-323-5p	CLIP-seq
MIRT1226385	hsa-miR-3619-5p	CLIP-seq
MIRT1226386	hsa-miR-3687	CLIP-seq
MIRT1226387	hsa-miR-4436b-3p	CLIP-seq
MIRT1226388	hsa-miR-4442	CLIP-seq
MIRT1226389	hsa-miR-450b-3p	CLIP-seq
MIRT1226390	hsa-miR-4520a-3p	CLIP-seq
MIRT1226391	hsa-miR-4685-5p	CLIP-seq
MIRT1226392	hsa-miR-4713-3p	CLIP-seq
MIRT1226393	hsa-miR-4755-3p	CLIP-seq
MIRT1226394	hsa-miR-4786-5p	CLIP-seq
MIRT1226395	hsa-miR-591	CLIP-seq
MIRT1226396	hsa-miR-638	CLIP-seq
MIRT1226397	hsa-miR-658	CLIP-seq
MIRT1226398	hsa-miR-761	CLIP-seq
MIRT1226399	hsa-miR-769-3p	CLIP-seq
MIRT2066293	hsa-miR-1184	CLIP-seq
MIRT2066294	hsa-miR-1205	CLIP-seq
MIRT2066295	hsa-miR-1226	CLIP-seq
MIRT2066296	hsa-miR-1276	CLIP-seq
MIRT2066297	hsa-miR-15a	CLIP-seq
MIRT2066298	hsa-miR-15b	CLIP-seq
MIRT2066299	hsa-miR-16	CLIP-seq
MIRT2066300	hsa-miR-1910	CLIP-seq
MIRT2066301	hsa-miR-195	CLIP-seq
MIRT2066302	hsa-miR-2278	CLIP-seq
MIRT2066303	hsa-miR-2355-5p	CLIP-seq
MIRT2066304	hsa-miR-2861	CLIP-seq
MIRT2066305	hsa-miR-3158-5p	CLIP-seq
MIRT2066306	hsa-miR-424	CLIP-seq
MIRT1226390	hsa-miR-4520a-3p	CLIP-seq
MIRT2066307	hsa-miR-4524	CLIP-seq
MIRT2066308	hsa-miR-4642	CLIP-seq
MIRT2066309	hsa-miR-4672	CLIP-seq
MIRT1226391	hsa-miR-4685-5p	CLIP-seq
MIRT1226392	hsa-miR-4713-3p	CLIP-seq
MIRT2066310	hsa-miR-497	CLIP-seq
MIRT2066311	hsa-miR-513b	CLIP-seq
MIRT2066312	hsa-miR-518a-5p	CLIP-seq
MIRT2066313	hsa-miR-527	CLIP-seq
MIRT2066295	hsa-miR-1226	CLIP-seq
MIRT2293779	hsa-miR-1229	CLIP-seq
MIRT2293780	hsa-miR-146b-3p	CLIP-seq
MIRT2066297	hsa-miR-15a	CLIP-seq
MIRT2066298	hsa-miR-15b	CLIP-seq
MIRT2066299	hsa-miR-16	CLIP-seq
MIRT2066301	hsa-miR-195	CLIP-seq
MIRT1226376	hsa-miR-214	CLIP-seq
MIRT2066302	hsa-miR-2278	CLIP-seq
MIRT2293781	hsa-miR-3150a-3p	CLIP-seq
MIRT2293782	hsa-miR-3175	CLIP-seq
MIRT2293783	hsa-miR-3184	CLIP-seq
MIRT1226385	hsa-miR-3619-5p	CLIP-seq
MIRT2293784	hsa-miR-3663-5p	CLIP-seq
MIRT2293785	hsa-miR-423-5p	CLIP-seq
MIRT2066306	hsa-miR-424	CLIP-seq
MIRT2293786	hsa-miR-4287	CLIP-seq
MIRT2293787	hsa-miR-4313	CLIP-seq
MIRT2293788	hsa-miR-4457	CLIP-seq
MIRT2293789	hsa-miR-4469	CLIP-seq
MIRT2293790	hsa-miR-4488	CLIP-seq
MIRT1226390	hsa-miR-4520a-3p	CLIP-seq
MIRT2293791	hsa-miR-4522	CLIP-seq
MIRT2066307	hsa-miR-4524	CLIP-seq
MIRT2293792	hsa-miR-4667-5p	CLIP-seq
MIRT2293793	hsa-miR-4685-3p	CLIP-seq
MIRT2293794	hsa-miR-4697-5p	CLIP-seq
MIRT2293795	hsa-miR-4700-5p	CLIP-seq
MIRT2293796	hsa-miR-4728-5p	CLIP-seq
MIRT2293797	hsa-miR-4731-3p	CLIP-seq
MIRT2293798	hsa-miR-4733-3p	CLIP-seq
MIRT2293799	hsa-miR-4801	CLIP-seq
MIRT2066310	hsa-miR-497	CLIP-seq
MIRT2293800	hsa-miR-503	CLIP-seq
MIRT2293801	hsa-miR-622	CLIP-seq
MIRT2293802	hsa-miR-646	CLIP-seq
MIRT1226398	hsa-miR-761	CLIP-seq
MIRT2293803	hsa-miR-939	CLIP-seq
MIRT2066293	hsa-miR-1184	CLIP-seq
MIRT2591861	hsa-miR-1202	CLIP-seq
MIRT2066294	hsa-miR-1205	CLIP-seq
MIRT2066295	hsa-miR-1226	CLIP-seq
MIRT2591862	hsa-miR-197	CLIP-seq
MIRT2066302	hsa-miR-2278	CLIP-seq
MIRT1226380	hsa-miR-2467-3p	CLIP-seq
MIRT2591863	hsa-miR-30a	CLIP-seq
MIRT2591864	hsa-miR-30b	CLIP-seq
MIRT2591865	hsa-miR-30c	CLIP-seq
MIRT2591866	hsa-miR-30d	CLIP-seq
MIRT2591867	hsa-miR-30e	CLIP-seq
MIRT2066305	hsa-miR-3158-5p	CLIP-seq
MIRT2591868	hsa-miR-3194-5p	CLIP-seq
MIRT2591869	hsa-miR-3607-3p	CLIP-seq
MIRT2591870	hsa-miR-3654	CLIP-seq
MIRT2591871	hsa-miR-3678-3p	CLIP-seq
MIRT2591872	hsa-miR-378	CLIP-seq
MIRT2591873	hsa-miR-378b	CLIP-seq
MIRT2591874	hsa-miR-378c	CLIP-seq
MIRT2591875	hsa-miR-378d	CLIP-seq
MIRT2591876	hsa-miR-378e	CLIP-seq
MIRT2591877	hsa-miR-378f	CLIP-seq
MIRT2591878	hsa-miR-378h	CLIP-seq
MIRT2591879	hsa-miR-378i	CLIP-seq
MIRT2591880	hsa-miR-3922-5p	CLIP-seq
MIRT2591881	hsa-miR-3972	CLIP-seq
MIRT2591882	hsa-miR-422a	CLIP-seq
MIRT2591883	hsa-miR-4323	CLIP-seq
MIRT2293788	hsa-miR-4457	CLIP-seq
MIRT2591884	hsa-miR-4494	CLIP-seq
MIRT2591885	hsa-miR-4519	CLIP-seq
MIRT2591886	hsa-miR-4640-3p	CLIP-seq
MIRT2591887	hsa-miR-4676-5p	CLIP-seq
MIRT2293798	hsa-miR-4733-3p	CLIP-seq
MIRT2591888	hsa-miR-4753-5p	CLIP-seq
MIRT2591889	hsa-miR-4758-3p	CLIP-seq
MIRT2591890	hsa-miR-4763-5p	CLIP-seq
MIRT2591891	hsa-miR-4776-3p	CLIP-seq
MIRT2591892	hsa-miR-4798-3p	CLIP-seq
MIRT2591893	hsa-miR-5096	CLIP-seq
MIRT2066311	hsa-miR-513b	CLIP-seq
MIRT2591894	hsa-miR-515-3p	CLIP-seq
MIRT2591895	hsa-miR-519e	CLIP-seq
MIRT2591896	hsa-miR-550a	CLIP-seq
MIRT2591897	hsa-miR-575	CLIP-seq
MIRT2293801	hsa-miR-622	CLIP-seq
MIRT2066295	hsa-miR-1226	CLIP-seq
MIRT2591862	hsa-miR-197	CLIP-seq
MIRT2591868	hsa-miR-3194-5p	CLIP-seq
MIRT2687692	hsa-miR-3202	CLIP-seq
MIRT2591870	hsa-miR-3654	CLIP-seq
MIRT2591872	hsa-miR-378	CLIP-seq
MIRT2591873	hsa-miR-378b	CLIP-seq
MIRT2591874	hsa-miR-378c	CLIP-seq
MIRT2591875	hsa-miR-378d	CLIP-seq
MIRT2591876	hsa-miR-378e	CLIP-seq
MIRT2591877	hsa-miR-378f	CLIP-seq
MIRT2591878	hsa-miR-378h	CLIP-seq
MIRT2591879	hsa-miR-378i	CLIP-seq
MIRT2591880	hsa-miR-3922-5p	CLIP-seq
MIRT2591882	hsa-miR-422a	CLIP-seq
MIRT2293788	hsa-miR-4457	CLIP-seq
MIRT2687693	hsa-miR-4533	CLIP-seq
MIRT2293798	hsa-miR-4733-3p	CLIP-seq
MIRT2591888	hsa-miR-4753-5p	CLIP-seq
MIRT2687694	hsa-miR-4790-3p	CLIP-seq
MIRT2591892	hsa-miR-4798-3p	CLIP-seq
MIRT2591893	hsa-miR-5096	CLIP-seq
MIRT2066311	hsa-miR-513b	CLIP-seq

Transcription factors

Transcription factor	Regulation	Reference
HIF1A	Unknown	16311927

Gene ontology (GO)

GO ID	Ontology	Definition	Evidence	Reference
GO:0003873	Function	6-phosphofructo-2-kinase activity	IBA	21873635
GO:0003873	Function	6-phosphofructo-2-kinase activity	NAS	10095107
GO:0004331	Function	Fructose-2,6-bisphosphate 2-phosphatase activity	IBA	21873635
GO:0005515	Function	Protein binding	IPI	32296183
GO:0005524	Function	ATP binding	IEA
GO:0005829	Component	Cytosol	IBA	21873635
GO:0005829	Component	Cytosol	TAS
GO:0006000	Process	Fructose metabolic process	IEA
GO:0006003	Process	Fructose 2,6-bisphosphate metabolic process	IBA	21873635
GO:0016311	Process	Dephosphorylation	IEA
GO:0045821	Process	Positive regulation of glycolytic process	TAS
GO:0046835	Process	Carbohydrate phosphorylation	IEA

Other IDs

• MIM: 605320
• HGNC: 8875
• e!Ensembl: ENSG00000114268

Protein

• UniProt ID: Q16877
• Protein name: 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4 (6PF-2-K/Fru-2,6-P2ase 4) (PFK/FBPase 4) (6PF-2-K/Fru-2,6-P2ase testis-type isozyme) [Includes: 6-phosphofructo-2-kinase (EC 2.7.1.105); Fructose-2,6-bisphosphatase (EC 3.1.3.46)]
• Protein function: Synthesis and degradation of fructose 2,6-bisphosphate.
• Family and domains:

  Pfam

  Accession	ID	Position in sequence	Description	Type
  PF01591	6PF2K	28 → 249	6-phosphofructo-2-kinase	Domain
  PF00300	His_Phos_1	252 → 438	Histidine phosphatase superfamily (branch 1)	Domain

• Sequence:

  MASPRELTQNPLKKIWMPYSNGRPALHACQRGVCMTNCPTLIVMVGLPARGKTYISKKLT
  RYLNWIGVPTREFNVGQYRRDVVKTYKSFEFFLPDNEEGLKIRKQCALAALRDVRRFLSE
  EGGHVAVFDATNTTRERRATIFNFGEQNGYKTFFVESICVDPEVIAANIVQVKLGSPDYV
  NRDSDEATEDFMRRIECYENSYESLDEDLDRDLSYIKIMDVGQSYVVNRVADHIQSRIVY
  YLMNIHVTPRSIYLCRHGESELNLKGRIGGDPGLSPRGREFAKSLAQFISDQNIKDLKVW
  TSQMKRTIQTAEALGVPYEQWKVLNEIDAGVCEEMTYEEIQDNYPLEFALRDQDKYRYRY
  PKGESYEDLVQRLEPVIMELERQENVLVICHQAVMRCLLAYFLDKAAEQLPYLKCPLHTV
  LKLTPVAYGCKVESIFLNVAAVNTHRDRPQNVDISRPPEEALVTVPAHQ

• Sequence length: 469

Pathways

KEGG:

Fructose and mannose metabolism
Metabolic pathways
AMPK signaling pathway

Reactome:

Regulation of glycolysis by fructose 2,6-bisphosphate metabolism

Associated diseases

Causal
Disease name	Disease term	dbSNP ID	References
Breast cancer	Malignant neoplasm of breast	rs587776547, rs1137887, rs137853007, rs587776650, rs80359351, rs80359714, rs121917783, rs104886456, rs121964878, rs80359874, rs80357868, rs80357508, rs387906843, rs80357569, rs80358158, rs80357524, rs80357115, rs80357945, rs80357729, rs80357609, rs80357259, rs80357981, rs80358063, rs80357389, rs80356862, rs80359876, rs80357580, rs80358053, rs80358089, rs80187739, rs397507241, rs80358069, rs80357590, rs80357284, rs80357941, rs80359261, rs80359272, rs80359276, rs276174813, rs80358474, rs80359316, rs1555282969, rs80359388, rs80359499, rs80359505, rs80359520, rs80359526, rs80359533, rs56253082, rs80358824, rs80359554, rs80359636, rs80359651, rs80359659, rs80359011, rs80359012, rs80359013, rs80359718, rs397507410, rs81002812, rs80359730, rs80359152, rs80359159, rs397507419, rs28897759, rs80359211, rs80359775, rs397514577, rs397507584, rs80358435, rs80358456, rs80359340, rs80359343, rs80359365, rs80358579, rs397507670, rs80358593, rs80359406, rs80359444, rs80359454, rs276174853, rs276174854, rs80359483, rs80359537, rs80358815, rs80358843, rs80359558, rs80359560, rs80359594, rs80358893, rs28897743, rs397507900, rs397507906, rs397507918, rs80358971, rs80358981, rs397507941, rs80359030, rs80359035, rs41293511, rs397507396, rs81002806, rs80359112, rs397508006, rs81002893, rs45580035, rs80359760, rs397508051, rs80359772, rs4987049, rs80359777, rs80357770, rs397508867, rs62625303, rs397508874, rs80357506, rs80357287, rs273898674, rs80358042, rs80358083, rs80357058, rs41286296, rs80357960, rs80356945, rs80357223, rs386134270, rs80358116, rs80357856, rs80357424, rs397509050, rs80357485, rs80357966, rs397509067, rs80357310, rs80356866, rs80357260, rs80357437, rs80358023, rs80358086, rs80357133, rs80356993, rs80357997, rs80357239, rs80357227, rs397509243, rs80356969, rs80356959, rs63750617, rs63751319, rs587779315, rs200640585, rs398122546, rs80357543, rs398122687, rs80359328, rs398122779, rs398122783, rs62517194, rs80358029, rs515726060, rs180177103, rs180177111, rs180177133, rs587776527, rs180177135, rs180177136, rs515726117, rs587779813, rs587779909, rs587780024, rs587780100, rs28909982, rs121908698, rs180177100, rs587780210, rs587780240, rs587780639, rs587781269, rs587781353, rs587781471, rs587781658, rs587781697, rs587781730, rs587781894, rs587781948, rs587782005, rs587782011, rs200928781, rs587781558, rs370228071, rs587782245, rs587782401, rs180177110, rs587782504, rs72552322, rs587782531, rs587782620, rs587782680, rs587782774, rs587782818, rs730881411, rs730881389, rs564652222, rs397507768, rs587776419, rs730881868, rs730881940, rs56383036, rs758972589, rs201089102, rs730881348, rs786202608, rs786201886, rs786203318, rs786203775, rs786203714, rs786202033, rs750621215, rs786203884, rs786203650, rs772821016, rs863224521, rs864622223, rs864622655, rs375699023, rs876659572, rs768362387, rs876659535, rs876658957, rs483353072, rs876659435, rs267608041, rs876661113, rs730881369, rs878853535, rs772228129, rs878855122, rs760551339, rs80359596, rs397509222, rs886039630, rs886039683, rs886040828, rs587781799, rs886040374, rs886040649, rs397507967, rs878854957, rs886040043, rs1057517589, rs1060502769, rs866380588, rs863224765, rs1064793243, rs747563556, rs1555074976, rs1064795885, rs753961188, rs1064794708, rs869312772, rs1064793887, rs1131690820, rs1135401928, rs1135401868, rs1135401859, rs1553370324, rs397507630, rs1555283160, rs1555283251, rs1555283262, rs1555283361, rs1555286298, rs1555288462, rs886040950, rs1555289566, rs776323117, rs80357123, rs1555579627, rs1555580697, rs80358054, rs1555593302, rs1328985852, rs763470424, rs1555139694, rs878854697, rs1555461217, rs1555461765, rs774684620, rs766416564, rs1554558613, rs1305740166, rs1555461460, rs1555461407, rs1555461586, rs1555567202, rs1555607022, rs1555069815, rs1442299125, rs1474786480, rs1555084947, rs1555457867, rs141087784, rs1482641121, rs1564830522, rs1565469955, rs1565503137, rs864622613, rs755263466, rs757679199, rs1593903166, rs1597801649, rs1603293306, rs879253880, rs80358754, rs1597062038, rs45494092, rs1603275367, rs887358871, rs1597091518, rs1966967065, rs1064793049, rs2082872908, rs2085078278, rs2072475243