PDK4 (pyruvate dehydrogenase kinase 4)

Gene

• Entrez ID: 5166
• Gene name: Pyruvate dehydrogenase kinase 4
• Gene symbol: PDK4
• Synonyms: -
• Chromosome: 7
• Chromosome location: 7q21.3
• Summary: This gene is a member of the PDK/BCKDK protein kinase family and encodes a mitochondrial protein with a histidine kinase domain. This protein is located in the matrix of the mitrochondria and inhibits the pyruvate dehydrogenase complex by phosphorylating

miRNA

miRTarBase ID	miRNA	Experiments	Reference
MIRT018910	hsa-miR-335-5p	Microarray	18185580
MIRT027229	hsa-miR-103a-3p	Sequencing	20371350
MIRT030098	hsa-miR-26b-5p	Microarray	19088304
MIRT032003	hsa-miR-16-5p	Sequencing	20371350
MIRT734505	hsa-miR-152-3p	ELISA, Flow cytometry, Immunoprecipitaion (IP), Luciferase reporter assay, qRT-PCR, Western blotting	34165388
MIRT1222070	hsa-miR-421	CLIP-seq
MIRT1222071	hsa-miR-4272	CLIP-seq
MIRT2064337	hsa-miR-329	CLIP-seq
MIRT2064338	hsa-miR-362-3p	CLIP-seq
MIRT2064339	hsa-miR-3667-3p	CLIP-seq
MIRT2064340	hsa-miR-3941	CLIP-seq
MIRT2064341	hsa-miR-466	CLIP-seq
MIRT2064342	hsa-miR-4672	CLIP-seq
MIRT2064343	hsa-miR-4768-5p	CLIP-seq
MIRT2064344	hsa-miR-579	CLIP-seq
MIRT2064345	hsa-miR-603	CLIP-seq
MIRT2292654	hsa-miR-338-3p	CLIP-seq
MIRT2292655	hsa-miR-4310	CLIP-seq
MIRT2292656	hsa-miR-4659a-3p	CLIP-seq
MIRT2292657	hsa-miR-4659b-3p	CLIP-seq
MIRT2064343	hsa-miR-4768-5p	CLIP-seq
MIRT2439325	hsa-miR-1208	CLIP-seq
MIRT2439326	hsa-miR-1224-3p	CLIP-seq
MIRT2439327	hsa-miR-1260	CLIP-seq
MIRT2439328	hsa-miR-1260b	CLIP-seq
MIRT2439329	hsa-miR-1280	CLIP-seq
MIRT2439330	hsa-miR-136	CLIP-seq
MIRT2439331	hsa-miR-141	CLIP-seq
MIRT2439332	hsa-miR-200a	CLIP-seq
MIRT2439333	hsa-miR-216b	CLIP-seq
MIRT2439334	hsa-miR-3117-3p	CLIP-seq
MIRT2439335	hsa-miR-3169	CLIP-seq
MIRT2439336	hsa-miR-3173-3p	CLIP-seq
MIRT2439337	hsa-miR-330-3p	CLIP-seq
MIRT2439338	hsa-miR-340	CLIP-seq
MIRT2439339	hsa-miR-3622b-5p	CLIP-seq
MIRT2439340	hsa-miR-3646	CLIP-seq
MIRT2439341	hsa-miR-3653	CLIP-seq
MIRT2439342	hsa-miR-3658	CLIP-seq
MIRT2439343	hsa-miR-371-5p	CLIP-seq
MIRT2439344	hsa-miR-371b-5p	CLIP-seq
MIRT2439345	hsa-miR-3920	CLIP-seq
MIRT2439346	hsa-miR-3973	CLIP-seq
MIRT2439347	hsa-miR-4422	CLIP-seq
MIRT2439348	hsa-miR-4428	CLIP-seq
MIRT2439349	hsa-miR-4462	CLIP-seq
MIRT2439350	hsa-miR-4477b	CLIP-seq
MIRT2439351	hsa-miR-4704-5p	CLIP-seq
MIRT2439352	hsa-miR-4720-5p	CLIP-seq
MIRT2439353	hsa-miR-4735-5p	CLIP-seq
MIRT2439354	hsa-miR-4759	CLIP-seq
MIRT2439355	hsa-miR-4762-3p	CLIP-seq
MIRT2439356	hsa-miR-4795-3p	CLIP-seq
MIRT2439357	hsa-miR-4799-3p	CLIP-seq
MIRT2439358	hsa-miR-548a-5p	CLIP-seq
MIRT2439359	hsa-miR-548ab	CLIP-seq
MIRT2439360	hsa-miR-548ak	CLIP-seq
MIRT2439361	hsa-miR-548b-5p	CLIP-seq
MIRT2439362	hsa-miR-548c-5p	CLIP-seq
MIRT2439363	hsa-miR-548d-5p	CLIP-seq
MIRT2439364	hsa-miR-548h	CLIP-seq
MIRT2439365	hsa-miR-548i	CLIP-seq
MIRT2439366	hsa-miR-548j	CLIP-seq
MIRT2439367	hsa-miR-548w	CLIP-seq
MIRT2439368	hsa-miR-548y	CLIP-seq
MIRT2439369	hsa-miR-555	CLIP-seq
MIRT2439370	hsa-miR-559	CLIP-seq
MIRT2439371	hsa-miR-580	CLIP-seq
MIRT2439372	hsa-miR-602	CLIP-seq
MIRT2439373	hsa-miR-626	CLIP-seq
MIRT2439374	hsa-miR-643	CLIP-seq
MIRT2439375	hsa-miR-668	CLIP-seq
MIRT2439331	hsa-miR-141	CLIP-seq
MIRT2439332	hsa-miR-200a	CLIP-seq
MIRT2439351	hsa-miR-4704-5p	CLIP-seq
MIRT2590642	hsa-miR-103a	CLIP-seq
MIRT2590643	hsa-miR-106a	CLIP-seq
MIRT2590644	hsa-miR-106b	CLIP-seq
MIRT2590645	hsa-miR-107	CLIP-seq
MIRT2439325	hsa-miR-1208	CLIP-seq
MIRT2439326	hsa-miR-1224-3p	CLIP-seq
MIRT2439327	hsa-miR-1260	CLIP-seq
MIRT2439328	hsa-miR-1260b	CLIP-seq
MIRT2590646	hsa-miR-1271	CLIP-seq
MIRT2439329	hsa-miR-1280	CLIP-seq
MIRT2590647	hsa-miR-129-5p	CLIP-seq
MIRT2439331	hsa-miR-141	CLIP-seq
MIRT2590648	hsa-miR-15a	CLIP-seq
MIRT2590649	hsa-miR-15b	CLIP-seq
MIRT2590650	hsa-miR-16	CLIP-seq
MIRT2590651	hsa-miR-17	CLIP-seq
MIRT2590652	hsa-miR-182	CLIP-seq
MIRT2590653	hsa-miR-195	CLIP-seq
MIRT2439332	hsa-miR-200a	CLIP-seq
MIRT2590654	hsa-miR-20a	CLIP-seq
MIRT2590655	hsa-miR-20b	CLIP-seq
MIRT2590656	hsa-miR-28-5p	CLIP-seq
MIRT2439334	hsa-miR-3117-3p	CLIP-seq
MIRT2590657	hsa-miR-3139	CLIP-seq
MIRT2590658	hsa-miR-3145-3p	CLIP-seq
MIRT2439335	hsa-miR-3169	CLIP-seq
MIRT2439337	hsa-miR-330-3p	CLIP-seq
MIRT2439338	hsa-miR-340	CLIP-seq
MIRT2590659	hsa-miR-3591-5p	CLIP-seq
MIRT2590660	hsa-miR-3609	CLIP-seq
MIRT2590661	hsa-miR-361-5p	CLIP-seq
MIRT2439341	hsa-miR-3653	CLIP-seq
MIRT2439342	hsa-miR-3658	CLIP-seq
MIRT2590662	hsa-miR-3682-3p	CLIP-seq
MIRT2439343	hsa-miR-371-5p	CLIP-seq
MIRT2439344	hsa-miR-371b-5p	CLIP-seq
MIRT2439346	hsa-miR-3973	CLIP-seq
MIRT2590663	hsa-miR-424	CLIP-seq
MIRT2590664	hsa-miR-4257	CLIP-seq
MIRT2439349	hsa-miR-4462	CLIP-seq
MIRT2590665	hsa-miR-4502	CLIP-seq
MIRT2439351	hsa-miR-4704-5p	CLIP-seq
MIRT2439353	hsa-miR-4735-5p	CLIP-seq
MIRT2439354	hsa-miR-4759	CLIP-seq
MIRT2439355	hsa-miR-4762-3p	CLIP-seq
MIRT2439356	hsa-miR-4795-3p	CLIP-seq
MIRT2590666	hsa-miR-4796-3p	CLIP-seq
MIRT2590667	hsa-miR-497	CLIP-seq
MIRT2590668	hsa-miR-519d	CLIP-seq
MIRT2590669	hsa-miR-548a-3p	CLIP-seq
MIRT2439358	hsa-miR-548a-5p	CLIP-seq
MIRT2439359	hsa-miR-548ab	CLIP-seq
MIRT2590670	hsa-miR-548ac	CLIP-seq
MIRT2590671	hsa-miR-548ae	CLIP-seq
MIRT2590672	hsa-miR-548ah	CLIP-seq
MIRT2590673	hsa-miR-548aj	CLIP-seq
MIRT2439360	hsa-miR-548ak	CLIP-seq
MIRT2590674	hsa-miR-548am	CLIP-seq
MIRT2439361	hsa-miR-548b-5p	CLIP-seq
MIRT2439362	hsa-miR-548c-5p	CLIP-seq
MIRT2590675	hsa-miR-548d-3p	CLIP-seq
MIRT2439363	hsa-miR-548d-5p	CLIP-seq
MIRT2590676	hsa-miR-548e	CLIP-seq
MIRT2590677	hsa-miR-548f	CLIP-seq
MIRT2439364	hsa-miR-548h	CLIP-seq
MIRT2439365	hsa-miR-548i	CLIP-seq
MIRT2439366	hsa-miR-548j	CLIP-seq
MIRT2439367	hsa-miR-548w	CLIP-seq
MIRT2590678	hsa-miR-548x	CLIP-seq
MIRT2439368	hsa-miR-548y	CLIP-seq
MIRT2590679	hsa-miR-548z	CLIP-seq
MIRT2439369	hsa-miR-555	CLIP-seq
MIRT2439370	hsa-miR-559	CLIP-seq
MIRT2590680	hsa-miR-595	CLIP-seq
MIRT2439372	hsa-miR-602	CLIP-seq
MIRT2590681	hsa-miR-648	CLIP-seq
MIRT2439375	hsa-miR-668	CLIP-seq
MIRT2590682	hsa-miR-708	CLIP-seq
MIRT2590683	hsa-miR-93	CLIP-seq
MIRT2590684	hsa-miR-96	CLIP-seq
MIRT2590642	hsa-miR-103a	CLIP-seq
MIRT2590643	hsa-miR-106a	CLIP-seq
MIRT2590644	hsa-miR-106b	CLIP-seq
MIRT2590645	hsa-miR-107	CLIP-seq
MIRT2439325	hsa-miR-1208	CLIP-seq
MIRT2439326	hsa-miR-1224-3p	CLIP-seq
MIRT2439327	hsa-miR-1260	CLIP-seq
MIRT2439328	hsa-miR-1260b	CLIP-seq
MIRT2439329	hsa-miR-1280	CLIP-seq
MIRT2590648	hsa-miR-15a	CLIP-seq
MIRT2590649	hsa-miR-15b	CLIP-seq
MIRT2590650	hsa-miR-16	CLIP-seq
MIRT2590651	hsa-miR-17	CLIP-seq
MIRT2590653	hsa-miR-195	CLIP-seq
MIRT2590654	hsa-miR-20a	CLIP-seq
MIRT2590655	hsa-miR-20b	CLIP-seq
MIRT2590656	hsa-miR-28-5p	CLIP-seq
MIRT2439334	hsa-miR-3117-3p	CLIP-seq
MIRT2590657	hsa-miR-3139	CLIP-seq
MIRT2439335	hsa-miR-3169	CLIP-seq
MIRT2439337	hsa-miR-330-3p	CLIP-seq
MIRT2439338	hsa-miR-340	CLIP-seq
MIRT2590659	hsa-miR-3591-5p	CLIP-seq
MIRT2590660	hsa-miR-3609	CLIP-seq
MIRT2590661	hsa-miR-361-5p	CLIP-seq
MIRT2439341	hsa-miR-3653	CLIP-seq
MIRT2439342	hsa-miR-3658	CLIP-seq
MIRT2590662	hsa-miR-3682-3p	CLIP-seq
MIRT2439343	hsa-miR-371-5p	CLIP-seq
MIRT2439344	hsa-miR-371b-5p	CLIP-seq
MIRT2590663	hsa-miR-424	CLIP-seq
MIRT2590664	hsa-miR-4257	CLIP-seq
MIRT2439349	hsa-miR-4462	CLIP-seq
MIRT2590665	hsa-miR-4502	CLIP-seq
MIRT2439353	hsa-miR-4735-5p	CLIP-seq
MIRT2439354	hsa-miR-4759	CLIP-seq
MIRT2439355	hsa-miR-4762-3p	CLIP-seq
MIRT2439356	hsa-miR-4795-3p	CLIP-seq
MIRT2590666	hsa-miR-4796-3p	CLIP-seq
MIRT2590667	hsa-miR-497	CLIP-seq
MIRT2590668	hsa-miR-519d	CLIP-seq
MIRT2590669	hsa-miR-548a-3p	CLIP-seq
MIRT2590670	hsa-miR-548ac	CLIP-seq
MIRT2590671	hsa-miR-548ae	CLIP-seq
MIRT2590672	hsa-miR-548ah	CLIP-seq
MIRT2590673	hsa-miR-548aj	CLIP-seq
MIRT2590674	hsa-miR-548am	CLIP-seq
MIRT2590675	hsa-miR-548d-3p	CLIP-seq
MIRT2590676	hsa-miR-548e	CLIP-seq
MIRT2590677	hsa-miR-548f	CLIP-seq
MIRT2590678	hsa-miR-548x	CLIP-seq
MIRT2590679	hsa-miR-548z	CLIP-seq
MIRT2439369	hsa-miR-555	CLIP-seq
MIRT2590680	hsa-miR-595	CLIP-seq
MIRT2439372	hsa-miR-602	CLIP-seq
MIRT2590681	hsa-miR-648	CLIP-seq
MIRT2439375	hsa-miR-668	CLIP-seq
MIRT2590682	hsa-miR-708	CLIP-seq
MIRT2590683	hsa-miR-93	CLIP-seq

Transcription factors

Transcription factor	Regulation	Reference
E2F1	Repression	21625432
NFKB1	Repression	21625432
PPARA	Activation	15955060
PPARD	Activation	15955060
RELA	Repression	21625432

Gene ontology (GO)

GO ID	Ontology	Definition	Evidence	Reference
GO:0004672	Function	Protein kinase activity	IDA	18658136
GO:0004740	Function	Pyruvate dehydrogenase (acetyl-transferring) kinase activity	IBA	21873635
GO:0004740	Function	Pyruvate dehydrogenase (acetyl-transferring) kinase activity	ISS
GO:0005515	Function	Protein binding	IPI	32296183
GO:0005524	Function	ATP binding	IDA	18658136
GO:0005739	Component	Mitochondrion	IBA	21873635
GO:0005759	Component	Mitochondrial matrix	TAS
GO:0006006	Process	Glucose metabolic process	IEA
GO:0006468	Process	Protein phosphorylation	IBA	21873635
GO:0006468	Process	Protein phosphorylation	IEA
GO:0006885	Process	Regulation of pH	ISS
GO:0008286	Process	Insulin receptor signaling pathway	IMP	21852536
GO:0009267	Process	Cellular response to starvation	IDA	14966024
GO:0010510	Process	Regulation of acetyl-CoA biosynthetic process from pyruvate	IMP	21852536
GO:0010510	Process	Regulation of acetyl-CoA biosynthetic process from pyruvate	TAS
GO:0010565	Process	Regulation of cellular ketone metabolic process	ISS
GO:0010906	Process	Regulation of glucose metabolic process	IBA	21873635
GO:0010906	Process	Regulation of glucose metabolic process	IMP	21852536
GO:0042304	Process	Regulation of fatty acid biosynthetic process	IMP	21852536
GO:0042593	Process	Glucose homeostasis	ISS
GO:0042594	Process	Response to starvation	ISS
GO:0045124	Process	Regulation of bone resorption	IEA
GO:0046320	Process	Regulation of fatty acid oxidation	ISS
GO:0071398	Process	Cellular response to fatty acid	IMP	20715114
GO:0072593	Process	Reactive oxygen species metabolic process	IMP	21852536
GO:2000811	Process	Negative regulation of anoikis	IMP	21852536

Other IDs

• MIM: 602527
• HGNC: 8812
• e!Ensembl: ENSG00000004799

Protein

• UniProt ID: Q16654
• Protein name: [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 4, mitochondrial (EC 2.7.11.2) (Pyruvate dehydrogenase kinase isoform 4)
• Protein function: Kinase that plays a key role in regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates meta
• PDB: 2E0A, 2ZDX, 2ZDY, 2ZKJ, 3D2R, 7EAT, 7EBB, 7EBG
• Family and domains:

  Pfam

  Accession	ID	Position in sequence	Description	Type
  PF10436	BCDHK_Adom3	34 → 195	Mitochondrial branched-chain alpha-ketoacid dehydrogenase kinase	Family
  PF02518	HATPase_c	243 → 368	Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase	Domain

• Sequence:

  MKAARFVLRSAGSLNGAGLVPREVEHFSRYSPSPLSMKQLLDFGSENACERTSFAFLRQE
  LPVRLANILKEIDILPTQLVNTSSVQLVKSWYIQSLMDLVEFHEKSPDDQKALSDFVDTL
  IKVRNRHHNVVPTMAQGIIEYKDACTVDPVTNQNLQYFLDRFYMNRISTRMLMNQHILIF
  SDSQTGNPSHIGSIDPNCDVVAVVQDAFECSRMLCDQYYLSSPELKLTQVNGKFPDQPIH
  IVYVPSHLHHMLFELFKNAMRATVEHQENQPSLTPIEVIVVLGKEDLTIKISDRGGGVPL
  RIIDRLFSYTYSTAPTPVMDNSRNAPLAGFGYGLPISRLYAKYFQGDLNLYSLSGYGTDA
  IIYLKALSSESIEKLPVFNKSAFKHYQMSSEADDWCIPSREPKNLAKEVAM

• Sequence length: 411

Pathways

KEGG:

Diabetic cardiomyopathy

Reactome:

Regulation of pyruvate dehydrogenase (PDH) complex
Signaling by Retinoic Acid

Associated diseases

Unknown
Disease name	Disease term	dbSNP ID	References
Liver carcinoma	Liver carcinoma		28003426
Myocardial ischemia	Myocardial Ischemia		16214533
Non-alcoholic fatty liver disease	Non-alcoholic Fatty Liver Disease, Nonalcoholic Steatohepatitis		29289645