CYB5R4 (cytochrome b5 reductase 4)

Gene

• Entrez ID: 51167
• Gene name: Cytochrome b5 reductase 4
• Gene symbol: CYB5R4
• Synonyms: NCB5OR, cb5/cb5R, dJ676J13.1
• Chromosome: 6
• Chromosome location: 6q14.2
• Summary: NCB5OR is a flavohemoprotein that contains functional domains found in both cytochrome b5 (CYB5A; MIM 613218) and CYB5 reductase (CYB5R3; MIM 613213) (Zhu et al., 1999 [PubMed 10611283]).[supplied by OMIM, Jan 2010]

miRNA

miRTarBase ID	miRNA	Experiments	Reference
MIRT004438	hsa-miR-373-3p	Microarray	15685193
MIRT019387	hsa-miR-148b-3p	Microarray	17612493
MIRT004438	hsa-miR-373-3p	Microarray	15685193
MIRT029327	hsa-miR-26b-5p	Microarray	19088304
MIRT713699	hsa-miR-1250-3p	HITS-CLIP	19536157
MIRT713698	hsa-miR-6748-3p	HITS-CLIP	19536157
MIRT713697	hsa-miR-4421	HITS-CLIP	19536157
MIRT713696	hsa-miR-5699-3p	HITS-CLIP	19536157
MIRT713695	hsa-miR-1199-5p	HITS-CLIP	19536157
MIRT713694	hsa-miR-6751-3p	HITS-CLIP	19536157
MIRT713693	hsa-miR-3182	HITS-CLIP	19536157
MIRT713692	hsa-miR-4778-3p	HITS-CLIP	19536157
MIRT713691	hsa-miR-1225-3p	HITS-CLIP	19536157
MIRT713690	hsa-miR-1233-3p	HITS-CLIP	19536157
MIRT713689	hsa-miR-6740-3p	HITS-CLIP	19536157
MIRT713688	hsa-miR-4303	HITS-CLIP	19536157
MIRT713687	hsa-miR-3074-5p	HITS-CLIP	19536157
MIRT713686	hsa-miR-7152-5p	HITS-CLIP	19536157
MIRT713685	hsa-miR-4659a-3p	HITS-CLIP	19536157
MIRT713684	hsa-miR-4659b-3p	HITS-CLIP	19536157
MIRT567871	hsa-miR-32-3p	PAR-CLIP	20371350
MIRT567870	hsa-miR-3185	PAR-CLIP	20371350
MIRT567869	hsa-miR-555	PAR-CLIP	20371350
MIRT567868	hsa-miR-3201	PAR-CLIP	20371350
MIRT713699	hsa-miR-1250-3p	HITS-CLIP	19536157
MIRT713698	hsa-miR-6748-3p	HITS-CLIP	19536157
MIRT713697	hsa-miR-4421	HITS-CLIP	19536157
MIRT713696	hsa-miR-5699-3p	HITS-CLIP	19536157
MIRT713695	hsa-miR-1199-5p	HITS-CLIP	19536157
MIRT713694	hsa-miR-6751-3p	HITS-CLIP	19536157
MIRT713693	hsa-miR-3182	HITS-CLIP	19536157
MIRT713692	hsa-miR-4778-3p	HITS-CLIP	19536157
MIRT713691	hsa-miR-1225-3p	HITS-CLIP	19536157
MIRT713690	hsa-miR-1233-3p	HITS-CLIP	19536157
MIRT713689	hsa-miR-6740-3p	HITS-CLIP	19536157
MIRT713688	hsa-miR-4303	HITS-CLIP	19536157
MIRT713687	hsa-miR-3074-5p	HITS-CLIP	19536157
MIRT713686	hsa-miR-7152-5p	HITS-CLIP	19536157
MIRT713685	hsa-miR-4659a-3p	HITS-CLIP	19536157
MIRT713684	hsa-miR-4659b-3p	HITS-CLIP	19536157
MIRT567871	hsa-miR-32-3p	PAR-CLIP	20371350
MIRT567870	hsa-miR-3185	PAR-CLIP	20371350
MIRT567869	hsa-miR-555	PAR-CLIP	20371350
MIRT567868	hsa-miR-3201	PAR-CLIP	20371350
MIRT919648	hsa-miR-1200	CLIP-seq
MIRT919649	hsa-miR-1245	CLIP-seq
MIRT919650	hsa-miR-130a	CLIP-seq
MIRT919651	hsa-miR-130b	CLIP-seq
MIRT919652	hsa-miR-132	CLIP-seq
MIRT919653	hsa-miR-137	CLIP-seq
MIRT919654	hsa-miR-148a	CLIP-seq
MIRT919655	hsa-miR-148b	CLIP-seq
MIRT919656	hsa-miR-152	CLIP-seq
MIRT919657	hsa-miR-212	CLIP-seq
MIRT919658	hsa-miR-301a	CLIP-seq
MIRT919659	hsa-miR-301b	CLIP-seq
MIRT919660	hsa-miR-3120-3p	CLIP-seq
MIRT919661	hsa-miR-329	CLIP-seq
MIRT919662	hsa-miR-3617	CLIP-seq
MIRT919663	hsa-miR-362-3p	CLIP-seq
MIRT919664	hsa-miR-3646	CLIP-seq
MIRT919665	hsa-miR-3653	CLIP-seq
MIRT919666	hsa-miR-3658	CLIP-seq
MIRT919667	hsa-miR-3666	CLIP-seq
MIRT919668	hsa-miR-369-5p	CLIP-seq
MIRT919669	hsa-miR-3941	CLIP-seq
MIRT919670	hsa-miR-4282	CLIP-seq
MIRT919671	hsa-miR-4295	CLIP-seq
MIRT919672	hsa-miR-4324	CLIP-seq
MIRT919673	hsa-miR-4424	CLIP-seq
MIRT919674	hsa-miR-4493	CLIP-seq
MIRT919675	hsa-miR-454	CLIP-seq
MIRT919676	hsa-miR-4639-5p	CLIP-seq
MIRT919677	hsa-miR-4670-3p	CLIP-seq
MIRT919678	hsa-miR-4682	CLIP-seq
MIRT919679	hsa-miR-4699-3p	CLIP-seq
MIRT919680	hsa-miR-4708-3p	CLIP-seq
MIRT919681	hsa-miR-4711-5p	CLIP-seq
MIRT919682	hsa-miR-4729	CLIP-seq
MIRT919683	hsa-miR-4738-3p	CLIP-seq
MIRT919684	hsa-miR-4742-3p	CLIP-seq
MIRT919685	hsa-miR-4778-5p	CLIP-seq
MIRT919686	hsa-miR-494	CLIP-seq
MIRT919687	hsa-miR-544	CLIP-seq
MIRT919688	hsa-miR-544b	CLIP-seq
MIRT919689	hsa-miR-548ag	CLIP-seq
MIRT919690	hsa-miR-548ai	CLIP-seq
MIRT919691	hsa-miR-579	CLIP-seq
MIRT919692	hsa-miR-603	CLIP-seq
MIRT919693	hsa-miR-641	CLIP-seq
MIRT919677	hsa-miR-4670-3p	CLIP-seq
MIRT1972628	hsa-miR-543	CLIP-seq

Gene ontology (GO)

GO ID	Ontology	Definition	Evidence	Reference
GO:0003032	Process	Detection of oxygen	NAS	10611283
GO:0004128	Function	Cytochrome-b5 reductase activity, acting on NAD(P)H	IBA	21873635
GO:0004128	Function	Cytochrome-b5 reductase activity, acting on NAD(P)H	IDA	10611283
GO:0005783	Component	Endoplasmic reticulum	IBA	21873635
GO:0005783	Component	Endoplasmic reticulum	IDA	15131110
GO:0005789	Component	Endoplasmic reticulum membrane	TAS
GO:0006091	Process	Generation of precursor metabolites and energy	IBA	21873635
GO:0006091	Process	Generation of precursor metabolites and energy	IDA	10611283
GO:0006801	Process	Superoxide metabolic process	IBA	21873635
GO:0006801	Process	Superoxide metabolic process	IDA	10611283
GO:0015701	Process	Bicarbonate transport	TAS
GO:0016174	Function	NAD(P)H oxidase H2O2-forming activity	IDA	10611283
GO:0016174	Function	NAD(P)H oxidase H2O2-forming activity	IDA	15131110
GO:0016653	Function	Oxidoreductase activity, acting on NAD(P)H, heme protein as acceptor	IDA	15131110
GO:0020037	Function	Heme binding	IBA	21873635
GO:0030073	Process	Insulin secretion	ISS
GO:0042593	Process	Glucose homeostasis	ISS
GO:0046677	Process	Response to antibiotic	ISS
GO:0046872	Function	Metal ion binding	IEA
GO:0048468	Process	Cell development	ISS
GO:0048471	Component	Perinuclear region of cytoplasm	IDA	10611283

Other IDs

• MIM: 608343
• HGNC: 20147
• e!Ensembl: ENSG00000065615

Protein

• UniProt ID: Q7L1T6
• Protein name: Cytochrome b5 reductase 4 (EC 1.6.2.2) (Flavohemoprotein b5/b5R) (b5+b5R) (N-terminal cytochrome b5 and cytochrome b5 oxidoreductase domain-containing protein) (cb5/cb5R)
• Protein function: NADH-cytochrome b5 reductase involved in endoplasmic reticulum stress response pathway. Plays a critical role in protecting pancreatic beta-cells against oxidant stress, possibly by protecting the cell from excess buildup of reactive oxygen spec
• PDB: 3LF5, 6MV1, 6MV2
• Family and domains:

  Pfam

  Accession	ID	Position in sequence	Description	Type
  PF00173	Cyt-b5	58 → 130	Cytochrome b5-like Heme/Steroid binding domain	Domain
  PF04969	CS	168 → 246	CS domain	Domain
  PF00970	FAD_binding_6	277 → 384	Oxidoreductase FAD-binding domain	Domain
  PF00175	NAD_binding_1	395 → 503	Oxidoreductase NAD-binding domain	Domain

• Sequence:

  MLNVPSQSFPAPRSQQRVASGGRSKVPLKQGRSLMDWIRLTKSGKDLTGLKGRLIEVTEE
  ELKKHNKKDDCWICIRGFVYNVSPYMEYHPGGEDELMRAAGSDGTELFDQVHRWVNYESM
  LKECLVGRMAIKPAVLKDYREEEKKVLNGMLPKSQVTDTLAKEGPSYPSYDWFQTDSLVT
  IAIYTKQKDINLDSIIVDHQNDSFRAETIIKDCLYLIHIGLSHEVQEDFSVRVVESVGKI
  EIVLQKKENTSWDFLGHPLKNHNSLIPRKDTGLYYRKCQLISKEDVTHDTRLFCLMLPPS
  THLQVPIGQHVYLKLPITGTEIVKPYTPVSGSLLSEFKEPVLPNNKYIYFLIKIYPTGLF
  TPELDRLQIGDFVSVSSPEGNFKISKFQELEDLFLLAAGTGFTPMVKILNYALTDIPSLR
  KVKLMFFNKTEDDIIWRSQLEKLAFKDKRLDVEFVLSAPISEWNGKQGHISPALLSEFLK
  RNLDKSKVLVCICGPVPFTEQGVRLLHDLNFSKNEIHSFTA

• Sequence length: 521

Pathways

KEGG:

Biosynthesis of various nucleotide sugars

Reactome:

Erythrocytes take up carbon dioxide and release oxygen

Associated diseases

Causal
Disease name	Disease term	dbSNP ID	References
Breast cancer	Malignant neoplasm of breast	rs587776547, rs1137887, rs137853007, rs587776650, rs80359351, rs80359714, rs121917783, rs104886456, rs121964878, rs80359874, rs80357868, rs80357508, rs387906843, rs80357569, rs80358158, rs80357524, rs80357115, rs80357945, rs80357729, rs80357609, rs80357259, rs80357981, rs80358063, rs80357389, rs80356862, rs80359876, rs80357580, rs80358053, rs80358089, rs80187739, rs397507241, rs80358069, rs80357590, rs80357284, rs80357941, rs80359261, rs80359272, rs80359276, rs276174813, rs80358474, rs80359316, rs1555282969, rs80359388, rs80359499, rs80359505, rs80359520, rs80359526, rs80359533, rs56253082, rs80358824, rs80359554, rs80359636, rs80359651, rs80359659, rs80359011, rs80359012, rs80359013, rs80359718, rs397507410, rs81002812, rs80359730, rs80359152, rs80359159, rs397507419, rs28897759, rs80359211, rs80359775, rs397514577, rs397507584, rs80358435, rs80358456, rs80359340, rs80359343, rs80359365, rs80358579, rs397507670, rs80358593, rs80359406, rs80359444, rs80359454, rs276174853, rs276174854, rs80359483, rs80359537, rs80358815, rs80358843, rs80359558, rs80359560, rs80359594, rs80358893, rs28897743, rs397507900, rs397507906, rs397507918, rs80358971, rs80358981, rs397507941, rs80359030, rs80359035, rs41293511, rs397507396, rs81002806, rs80359112, rs397508006, rs81002893, rs45580035, rs80359760, rs397508051, rs80359772, rs4987049, rs80359777, rs80357770, rs397508867, rs62625303, rs397508874, rs80357506, rs80357287, rs273898674, rs80358042, rs80358083, rs80357058, rs41286296, rs80357960, rs80356945, rs80357223, rs386134270, rs80358116, rs80357856, rs80357424, rs397509050, rs80357485, rs80357966, rs397509067, rs80357310, rs80356866, rs80357260, rs80357437, rs80358023, rs80358086, rs80357133, rs80356993, rs80357997, rs80357239, rs80357227, rs397509243, rs80356969, rs80356959, rs63750617, rs63751319, rs587779315, rs200640585, rs398122546, rs80357543, rs398122687, rs80359328, rs398122779, rs398122783, rs62517194, rs80358029, rs515726060, rs180177103, rs180177111, rs180177133, rs587776527, rs180177135, rs180177136, rs515726117, rs587779813, rs587779909, rs587780024, rs587780100, rs28909982, rs121908698, rs180177100, rs587780210, rs587780240, rs587780639, rs587781269, rs587781353, rs587781471, rs587781658, rs587781697, rs587781730, rs587781894, rs587781948, rs587782005, rs587782011, rs200928781, rs587781558, rs370228071, rs587782245, rs587782401, rs180177110, rs587782504, rs72552322, rs587782531, rs587782620, rs587782680, rs587782774, rs587782818, rs730881411, rs730881389, rs564652222, rs397507768, rs587776419, rs730881868, rs730881940, rs56383036, rs758972589, rs201089102, rs730881348, rs786202608, rs786201886, rs786203318, rs786203775, rs786203714, rs786202033, rs750621215, rs786203884, rs786203650, rs772821016, rs863224521, rs864622223, rs864622655, rs375699023, rs876659572, rs768362387, rs876659535, rs876658957, rs483353072, rs876659435, rs267608041, rs876661113, rs730881369, rs878853535, rs772228129, rs878855122, rs760551339, rs80359596, rs397509222, rs886039630, rs886039683, rs886040828, rs587781799, rs886040374, rs886040649, rs397507967, rs878854957, rs886040043, rs1057517589, rs1060502769, rs866380588, rs863224765, rs1064793243, rs747563556, rs1555074976, rs1064795885, rs753961188, rs1064794708, rs869312772, rs1064793887, rs1131690820, rs1135401928, rs1135401868, rs1135401859, rs1553370324, rs397507630, rs1555283160, rs1555283251, rs1555283262, rs1555283361, rs1555286298, rs1555288462, rs886040950, rs1555289566, rs776323117, rs80357123, rs1555579627, rs1555580697, rs80358054, rs1555593302, rs1328985852, rs763470424, rs1555139694, rs878854697, rs1555461217, rs1555461765, rs774684620, rs766416564, rs1554558613, rs1305740166, rs1555461460, rs1555461407, rs1555461586, rs1555567202, rs1555607022, rs1555069815, rs1442299125, rs1474786480, rs1555084947, rs1555457867, rs141087784, rs1482641121, rs1564830522, rs1565469955, rs1565503137, rs864622613, rs755263466, rs757679199, rs1593903166, rs1597801649, rs1603293306, rs879253880, rs80358754, rs1597062038, rs45494092, rs1603275367, rs887358871, rs1597091518, rs1966967065, rs1064793049, rs2082872908, rs2085078278, rs2072475243