ARHGEF4 (Rho guanine nucleotide exchange factor 4)

Gene

• Entrez ID: 50649
• Gene name: Rho guanine nucleotide exchange factor 4
• Gene symbol: ARHGEF4
• Synonyms: ASEF, ASEF1, GEF4, SMIM39, STM6
• Chromosome: 2
• Chromosome location: 2q21.1
• Summary: Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The protein encoded by this gene may form complex with G proteins and stimulate Rho-dependent sig

miRNA

miRTarBase ID	miRNA	Experiments	Reference
MIRT016987	hsa-miR-335-5p	Microarray	18185580
MIRT483087	hsa-miR-6507-5p	PAR-CLIP	23592263
MIRT483088	hsa-miR-513b-3p	PAR-CLIP	23592263
MIRT483085	hsa-miR-4761-5p	PAR-CLIP	23592263
MIRT483086	hsa-miR-4693-3p	PAR-CLIP	23592263
MIRT483087	hsa-miR-6507-5p	PAR-CLIP	23592263
MIRT483088	hsa-miR-513b-3p	PAR-CLIP	23592263
MIRT483085	hsa-miR-4761-5p	PAR-CLIP	23592263
MIRT483086	hsa-miR-4693-3p	PAR-CLIP	23592263
MIRT795953	hsa-miR-1207-5p	CLIP-seq
MIRT795954	hsa-miR-1256	CLIP-seq
MIRT795955	hsa-miR-136	CLIP-seq
MIRT795956	hsa-miR-1972	CLIP-seq
MIRT795957	hsa-miR-3160-3p	CLIP-seq
MIRT795958	hsa-miR-3679-3p	CLIP-seq
MIRT795959	hsa-miR-4252	CLIP-seq
MIRT795960	hsa-miR-4476	CLIP-seq
MIRT795961	hsa-miR-4487	CLIP-seq
MIRT795962	hsa-miR-4488	CLIP-seq
MIRT795963	hsa-miR-4493	CLIP-seq
MIRT795964	hsa-miR-4663	CLIP-seq
MIRT795965	hsa-miR-4697-5p	CLIP-seq
MIRT795966	hsa-miR-4717-5p	CLIP-seq
MIRT795967	hsa-miR-4720-5p	CLIP-seq
MIRT795968	hsa-miR-4736	CLIP-seq
MIRT795969	hsa-miR-4763-3p	CLIP-seq
MIRT795970	hsa-miR-4799-3p	CLIP-seq
MIRT795971	hsa-miR-515-5p	CLIP-seq
MIRT795972	hsa-miR-558	CLIP-seq
MIRT795973	hsa-miR-637	CLIP-seq
MIRT795974	hsa-miR-766	CLIP-seq
MIRT795975	hsa-miR-198	CLIP-seq
MIRT795976	hsa-miR-338-3p	CLIP-seq
MIRT795977	hsa-miR-4530	CLIP-seq
MIRT795978	hsa-miR-4709-3p	CLIP-seq
MIRT795953	hsa-miR-1207-5p	CLIP-seq
MIRT1935087	hsa-miR-134	CLIP-seq
MIRT795955	hsa-miR-136	CLIP-seq
MIRT1935088	hsa-miR-1914	CLIP-seq
MIRT1935089	hsa-miR-3118	CLIP-seq
MIRT1935090	hsa-miR-3141	CLIP-seq
MIRT1935091	hsa-miR-3182	CLIP-seq
MIRT795959	hsa-miR-4252	CLIP-seq
MIRT795962	hsa-miR-4488	CLIP-seq
MIRT795965	hsa-miR-4697-5p	CLIP-seq
MIRT795968	hsa-miR-4736	CLIP-seq
MIRT795969	hsa-miR-4763-3p	CLIP-seq
MIRT795971	hsa-miR-515-5p	CLIP-seq
MIRT1935092	hsa-miR-592	CLIP-seq
MIRT1935093	hsa-miR-597	CLIP-seq
MIRT795973	hsa-miR-637	CLIP-seq
MIRT1935094	hsa-miR-668	CLIP-seq
MIRT1935095	hsa-miR-943	CLIP-seq
MIRT2175020	hsa-miR-1207-3p	CLIP-seq
MIRT2175021	hsa-miR-1236	CLIP-seq
MIRT2175022	hsa-miR-1587	CLIP-seq
MIRT2175023	hsa-miR-2861	CLIP-seq
MIRT795957	hsa-miR-3160-3p	CLIP-seq
MIRT2175024	hsa-miR-378g	CLIP-seq
MIRT795961	hsa-miR-4487	CLIP-seq
MIRT2175025	hsa-miR-4492	CLIP-seq
MIRT2175026	hsa-miR-4498	CLIP-seq
MIRT2175027	hsa-miR-4656	CLIP-seq
MIRT795964	hsa-miR-4663	CLIP-seq
MIRT2175028	hsa-miR-4675	CLIP-seq
MIRT2175029	hsa-miR-4741	CLIP-seq
MIRT795972	hsa-miR-558	CLIP-seq
MIRT2175030	hsa-miR-661	CLIP-seq
MIRT2175031	hsa-miR-762	CLIP-seq
MIRT2441965	hsa-miR-1184	CLIP-seq
MIRT2441966	hsa-miR-3960	CLIP-seq
MIRT2441967	hsa-miR-644	CLIP-seq

Gene ontology (GO)

GO ID	Ontology	Definition	Evidence	Reference
GO:0005085	Function	Guanyl-nucleotide exchange factor activity	IMP	17599059
GO:0005515	Function	Protein binding	IPI	17588722, 32296183
GO:0005829	Component	Cytosol	TAS
GO:0007186	Process	G protein-coupled receptor signaling pathway	TAS
GO:0019904	Function	Protein domain specific binding	IPI	10947987
GO:0030032	Process	Lamellipodium assembly	IMP	17599059
GO:0032587	Component	Ruffle membrane	IEA
GO:0035556	Process	Intracellular signal transduction	IEA
GO:0043065	Process	Positive regulation of apoptotic process	TAS
GO:0046847	Process	Filopodium assembly	IMP	17599059
GO:0050790	Process	Regulation of catalytic activity	IEA
GO:0051056	Process	Regulation of small GTPase mediated signal transduction	TAS

Other IDs

• MIM: 605216
• HGNC: 684
• e!Ensembl: ENSG00000136002

Protein

• UniProt ID: Q9NR80
• Protein name: Rho guanine nucleotide exchange factor 4 (APC-stimulated guanine nucleotide exchange factor 1) (Asef) (Asef1)
• Protein function: Acts as a guanine nucleotide exchange factor (GEF) for RHOA, RAC1 and CDC42 GTPases. Binding of APC may activate RAC1 GEF activity. The APC-ARHGEF4 complex seems to be involved in cell migration as well as in E-cadherin-mediated cell-cell adhesi
• PDB: 2DX1, 2PZ1, 3NMX, 3NMZ
• Family and domains:

  Pfam

  Accession	ID	Position in sequence	Description	Type
  PF00018	SH3_1	200 → 245	SH3 domain	Domain
  PF00621	RhoGEF	288 → 466	RhoGEF domain	Domain
  PF00169	PH	500 → 604	PH domain	Domain

• Sequence:

  MPWEEPAGEKPSCSHSQKAFHMEPAQKPCFTTDMVTWALLCISAETVRGEAPSQPRGIPH
  RSPVSVDDLWLEKTQRKKLQKQAHVERRLHIGAVHKDGVKCWRKTIITSPESLNLPRRSH
  PLSQSAPTGLNHMGWPEHTPGTAMPDGALDTAVCADEVGSEEDLYDDLHSSSHHYSHPGG
  GGEQLAINELISDGSVVCAEALWDHVTMDDQELGFKAGDVIEVMDATNREWWWGRVADGE
  GWFPASFVRLRVNQDEPADDDAPLAGNSGAEDGGAEAQSSKDQMRTNVINEILSTERDYI
  KHLRDICEGYVRQCRKRADMFSEEQLRTIFGNIEDIYRCQKAFVKALEQRFNRERPHLSE
  LGACFLEHQADFQIYSEYCNNHPNACVELSRLTKLSKYVYFFEACRLLQKMIDISLDGFL
  LTPVQKICKYPLQLAELLKYTHPQHRDFKDVEAALHAMKNVAQLINERKRRLENIDKIAQ
  WQSSIEDWEGEDLLVRSSELIYSGELTRVTQPQAKSQQRMFFLFDHQLIYCKKDLLRRDV
  LYYKGRLDMDGLEVVDLEDGKDRDLHVSIKNAFRLHRGATGDSHLLCTRKPEQKQRWLKA
  FAREREQVQLDQETGFSITELQRKQAMLNASKQQVTGKPKAVGRPCYLTRQKHPALPSNR
  PQQQVLVLAEPRRKPSTFWHSISRLAPFRK

• Sequence length: 690

Pathways

KEGG:

Regulation of actin cytoskeleton

Reactome:

NRAGE signals death through JNK
Rho GTPase cycle
G alpha (12/13) signalling events

Associated diseases

Causal
Disease name	Disease term	dbSNP ID	References
Breast cancer	Malignant neoplasm of breast	rs587776547, rs1137887, rs137853007, rs587776650, rs80359351, rs80359714, rs121917783, rs104886456, rs121964878, rs80359874, rs80357868, rs80357508, rs387906843, rs80357569, rs80358158, rs80357524, rs80357115, rs80357945, rs80357729, rs80357609, rs80357259, rs80357981, rs80358063, rs80357389, rs80356862, rs80359876, rs80357580, rs80358053, rs80358089, rs80187739, rs397507241, rs80358069, rs80357590, rs80357284, rs80357941, rs80359261, rs80359272, rs80359276, rs276174813, rs80358474, rs80359316, rs1555282969, rs80359388, rs80359499, rs80359505, rs80359520, rs80359526, rs80359533, rs56253082, rs80358824, rs80359554, rs80359636, rs80359651, rs80359659, rs80359011, rs80359012, rs80359013, rs80359718, rs397507410, rs81002812, rs80359730, rs80359152, rs80359159, rs397507419, rs28897759, rs80359211, rs80359775, rs397514577, rs397507584, rs80358435, rs80358456, rs80359340, rs80359343, rs80359365, rs80358579, rs397507670, rs80358593, rs80359406, rs80359444, rs80359454, rs276174853, rs276174854, rs80359483, rs80359537, rs80358815, rs80358843, rs80359558, rs80359560, rs80359594, rs80358893, rs28897743, rs397507900, rs397507906, rs397507918, rs80358971, rs80358981, rs397507941, rs80359030, rs80359035, rs41293511, rs397507396, rs81002806, rs80359112, rs397508006, rs81002893, rs45580035, rs80359760, rs397508051, rs80359772, rs4987049, rs80359777, rs80357770, rs397508867, rs62625303, rs397508874, rs80357506, rs80357287, rs273898674, rs80358042, rs80358083, rs80357058, rs41286296, rs80357960, rs80356945, rs80357223, rs386134270, rs80358116, rs80357856, rs80357424, rs397509050, rs80357485, rs80357966, rs397509067, rs80357310, rs80356866, rs80357260, rs80357437, rs80358023, rs80358086, rs80357133, rs80356993, rs80357997, rs80357239, rs80357227, rs397509243, rs80356969, rs80356959, rs63750617, rs63751319, rs587779315, rs200640585, rs398122546, rs80357543, rs398122687, rs80359328, rs398122779, rs398122783, rs62517194, rs80358029, rs515726060, rs180177103, rs180177111, rs180177133, rs587776527, rs180177135, rs180177136, rs515726117, rs587779813, rs587779909, rs587780024, rs587780100, rs28909982, rs121908698, rs180177100, rs587780210, rs587780240, rs587780639, rs587781269, rs587781353, rs587781471, rs587781658, rs587781697, rs587781730, rs587781894, rs587781948, rs587782005, rs587782011, rs200928781, rs587781558, rs370228071, rs587782245, rs587782401, rs180177110, rs587782504, rs72552322, rs587782531, rs587782620, rs587782680, rs587782774, rs587782818, rs730881411, rs730881389, rs564652222, rs397507768, rs587776419, rs730881868, rs730881940, rs56383036, rs758972589, rs201089102, rs730881348, rs786202608, rs786201886, rs786203318, rs786203775, rs786203714, rs786202033, rs750621215, rs786203884, rs786203650, rs772821016, rs863224521, rs864622223, rs864622655, rs375699023, rs876659572, rs768362387, rs876659535, rs876658957, rs483353072, rs876659435, rs267608041, rs876661113, rs730881369, rs878853535, rs772228129, rs878855122, rs760551339, rs80359596, rs397509222, rs886039630, rs886039683, rs886040828, rs587781799, rs886040374, rs886040649, rs397507967, rs878854957, rs886040043, rs1057517589, rs1060502769, rs866380588, rs863224765, rs1064793243, rs747563556, rs1555074976, rs1064795885, rs753961188, rs1064794708, rs869312772, rs1064793887, rs1131690820, rs1135401928, rs1135401868, rs1135401859, rs1553370324, rs397507630, rs1555283160, rs1555283251, rs1555283262, rs1555283361, rs1555286298, rs1555288462, rs886040950, rs1555289566, rs776323117, rs80357123, rs1555579627, rs1555580697, rs80358054, rs1555593302, rs1328985852, rs763470424, rs1555139694, rs878854697, rs1555461217, rs1555461765, rs774684620, rs766416564, rs1554558613, rs1305740166, rs1555461460, rs1555461407, rs1555461586, rs1555567202, rs1555607022, rs1555069815, rs1442299125, rs1474786480, rs1555084947, rs1555457867, rs141087784, rs1482641121, rs1564830522, rs1565469955, rs1565503137, rs864622613, rs755263466, rs757679199, rs1593903166, rs1597801649, rs1603293306, rs879253880, rs80358754, rs1597062038, rs45494092, rs1603275367, rs887358871, rs1597091518, rs1966967065, rs1064793049, rs2082872908, rs2085078278, rs2072475243