NEDD9 (neural precursor cell expressed, developmentally down-regulated 9)

Gene

• Entrez ID: 4739
• Gene name: Neural precursor cell expressed, developmentally down-regulated 9
• Gene symbol: NEDD9
• Synonyms: CAS-L, CAS2, CASL, CASS2, HEF1
• Chromosome: 6
• Chromosome location: 6p24.2
• Summary: The protein encoded by this gene is a member of the CRK-associated substrates family. Members of this family are adhesion docking molecules that mediate protein-protein interactions for signal transduction pathways. This protein is a focal adhesion protei

miRNA

miRTarBase ID	miRNA	Experiments	Reference
MIRT006817	hsa-miR-18a-5p	qRT-PCR, Western blot	22308110
MIRT006889	hsa-miR-145-5p	Immunofluorescence, Immunohistochemistry, qRT-PCR, Western blot	22869051
MIRT006889	hsa-miR-145-5p	Luciferase reporter assay	23355420
MIRT047916	hsa-miR-30c-5p	CLASH	23622248
MIRT006889	hsa-miR-145-5p	Luciferase reporter assay, qRT-PCR	24384875
MIRT006889	hsa-miR-145-5p	Luciferase reporter assay, qRT-PCR	24384875
MIRT655917	hsa-miR-3912-3p	HITS-CLIP	23824327
MIRT655916	hsa-miR-3127-3p	HITS-CLIP	23824327
MIRT655915	hsa-miR-6756-3p	HITS-CLIP	23824327
MIRT655917	hsa-miR-3912-3p	HITS-CLIP	23824327
MIRT655916	hsa-miR-3127-3p	HITS-CLIP	23824327
MIRT655915	hsa-miR-6756-3p	HITS-CLIP	23824327
MIRT454817	hsa-miR-3609	PAR-CLIP	23592263
MIRT454815	hsa-miR-548ah-5p	PAR-CLIP	23592263
MIRT454814	hsa-miR-4506	PAR-CLIP	23592263
MIRT454813	hsa-miR-29a-3p	PAR-CLIP	23592263
MIRT454812	hsa-miR-29b-3p	PAR-CLIP	23592263
MIRT454811	hsa-miR-29c-3p	PAR-CLIP	23592263
MIRT454810	hsa-miR-6871-3p	PAR-CLIP	23592263
MIRT454809	hsa-miR-520g-3p	PAR-CLIP	23592263
MIRT454808	hsa-miR-520h	PAR-CLIP	23592263
MIRT454807	hsa-miR-5702	PAR-CLIP	23592263
MIRT454806	hsa-miR-6835-5p	PAR-CLIP	23592263
MIRT454805	hsa-miR-6752-5p	PAR-CLIP	23592263
MIRT454804	hsa-miR-6842-5p	PAR-CLIP	23592263
MIRT454803	hsa-miR-7110-5p	PAR-CLIP	23592263
MIRT454802	hsa-miR-5682	PAR-CLIP	23592263
MIRT454801	hsa-miR-4477a	PAR-CLIP	23592263
MIRT454800	hsa-miR-642a-5p	PAR-CLIP	23592263
MIRT454799	hsa-miR-6751-5p	PAR-CLIP	23592263
MIRT454798	hsa-miR-6803-5p	PAR-CLIP	23592263
MIRT440124	hsa-miR-218-5p	HITS-CLIP	23212916
MIRT440124	hsa-miR-218-5p	HITS-CLIP	23212916
MIRT655917	hsa-miR-3912-3p	HITS-CLIP	23824327
MIRT655916	hsa-miR-3127-3p	HITS-CLIP	23824327
MIRT655915	hsa-miR-6756-3p	HITS-CLIP	23824327
MIRT655917	hsa-miR-3912-3p	HITS-CLIP	23824327
MIRT655916	hsa-miR-3127-3p	HITS-CLIP	23824327
MIRT655915	hsa-miR-6756-3p	HITS-CLIP	23824327
MIRT454817	hsa-miR-3609	PAR-CLIP	23592263
MIRT454815	hsa-miR-548ah-5p	PAR-CLIP	23592263
MIRT454814	hsa-miR-4506	PAR-CLIP	23592263
MIRT454813	hsa-miR-29a-3p	PAR-CLIP	23592263
MIRT454812	hsa-miR-29b-3p	PAR-CLIP	23592263
MIRT454811	hsa-miR-29c-3p	PAR-CLIP	23592263
MIRT454810	hsa-miR-6871-3p	PAR-CLIP	23592263
MIRT454809	hsa-miR-520g-3p	PAR-CLIP	23592263
MIRT454808	hsa-miR-520h	PAR-CLIP	23592263
MIRT454807	hsa-miR-5702	PAR-CLIP	23592263
MIRT454806	hsa-miR-6835-5p	PAR-CLIP	23592263
MIRT454805	hsa-miR-6752-5p	PAR-CLIP	23592263
MIRT454804	hsa-miR-6842-5p	PAR-CLIP	23592263
MIRT454803	hsa-miR-7110-5p	PAR-CLIP	23592263
MIRT454802	hsa-miR-5682	PAR-CLIP	23592263
MIRT454801	hsa-miR-4477a	PAR-CLIP	23592263
MIRT454800	hsa-miR-642a-5p	PAR-CLIP	23592263
MIRT454799	hsa-miR-6751-5p	PAR-CLIP	23592263
MIRT454798	hsa-miR-6803-5p	PAR-CLIP	23592263
MIRT1179924	hsa-miR-105	CLIP-seq
MIRT1179925	hsa-miR-1321	CLIP-seq
MIRT1179926	hsa-miR-1915	CLIP-seq
MIRT1179927	hsa-miR-298	CLIP-seq
MIRT1179928	hsa-miR-302f	CLIP-seq
MIRT1179929	hsa-miR-3136-5p	CLIP-seq
MIRT1179930	hsa-miR-3163	CLIP-seq
MIRT1179931	hsa-miR-3612	CLIP-seq
MIRT1179932	hsa-miR-3662	CLIP-seq
MIRT1179933	hsa-miR-4270	CLIP-seq
MIRT1179934	hsa-miR-4323	CLIP-seq
MIRT1179935	hsa-miR-4439	CLIP-seq
MIRT1179936	hsa-miR-4441	CLIP-seq
MIRT1179937	hsa-miR-4513	CLIP-seq
MIRT1179938	hsa-miR-4699-5p	CLIP-seq
MIRT1179939	hsa-miR-4719	CLIP-seq
MIRT1179940	hsa-miR-4739	CLIP-seq
MIRT1179941	hsa-miR-4756-5p	CLIP-seq
MIRT1179942	hsa-miR-4762-3p	CLIP-seq
MIRT1179943	hsa-miR-4766-5p	CLIP-seq
MIRT1179944	hsa-miR-650	CLIP-seq
MIRT1179945	hsa-miR-943	CLIP-seq
MIRT1179946	hsa-miR-34b	CLIP-seq
MIRT1179947	hsa-miR-3921	CLIP-seq
MIRT1179948	hsa-miR-4251	CLIP-seq
MIRT1179949	hsa-miR-4329	CLIP-seq
MIRT1179950	hsa-miR-4639-3p	CLIP-seq
MIRT1179951	hsa-miR-4653-5p	CLIP-seq
MIRT1179952	hsa-miR-4714-5p	CLIP-seq
MIRT2052033	hsa-miR-18a	CLIP-seq
MIRT2052034	hsa-miR-18b	CLIP-seq
MIRT2052035	hsa-miR-203	CLIP-seq
MIRT2052036	hsa-miR-4729	CLIP-seq
MIRT2052037	hsa-miR-4735-3p	CLIP-seq
MIRT1179932	hsa-miR-3662	CLIP-seq
MIRT2281397	hsa-miR-1266	CLIP-seq
MIRT2281398	hsa-miR-1294	CLIP-seq
MIRT2281399	hsa-miR-144	CLIP-seq
MIRT2052033	hsa-miR-18a	CLIP-seq
MIRT2052034	hsa-miR-18b	CLIP-seq
MIRT2281400	hsa-miR-3150b-3p	CLIP-seq
MIRT2281401	hsa-miR-3688-3p	CLIP-seq
MIRT2281402	hsa-miR-4316	CLIP-seq
MIRT2281403	hsa-miR-4328	CLIP-seq
MIRT2281404	hsa-miR-4518	CLIP-seq
MIRT2281405	hsa-miR-4652-3p	CLIP-seq
MIRT2281406	hsa-miR-4689	CLIP-seq
MIRT2052037	hsa-miR-4735-3p	CLIP-seq
MIRT2281407	hsa-miR-4760-3p	CLIP-seq
MIRT2281408	hsa-miR-4784	CLIP-seq
MIRT2281409	hsa-miR-510	CLIP-seq
MIRT2281410	hsa-miR-520d-5p	CLIP-seq
MIRT2281411	hsa-miR-524-5p	CLIP-seq
MIRT2281412	hsa-miR-576-3p	CLIP-seq
MIRT2281412	hsa-miR-576-3p	CLIP-seq
MIRT2431535	hsa-miR-3157-5p	CLIP-seq
MIRT2431536	hsa-miR-4691-3p	CLIP-seq
MIRT2431537	hsa-miR-4705	CLIP-seq
MIRT2579581	hsa-miR-1207-3p	CLIP-seq
MIRT2579582	hsa-miR-4307	CLIP-seq
MIRT2579583	hsa-miR-4774-5p	CLIP-seq
MIRT2579584	hsa-miR-567	CLIP-seq
MIRT2579581	hsa-miR-1207-3p	CLIP-seq
MIRT2579582	hsa-miR-4307	CLIP-seq
MIRT2579583	hsa-miR-4774-5p	CLIP-seq
MIRT2579584	hsa-miR-567	CLIP-seq

Gene ontology (GO)

GO ID	Ontology	Definition	Evidence	Reference
GO:0000922	Component	Spindle pole	IEA
GO:0005515	Function	Protein binding	IPI	11827972, 21516116, 25416956, 28514442
GO:0005634	Component	Nucleus	TAS	8668148
GO:0005654	Component	Nucleoplasm	IDA
GO:0005737	Component	Cytoplasm	IBA	21873635
GO:0005794	Component	Golgi apparatus	IEA
GO:0005819	Component	Spindle	TAS	9584194
GO:0005829	Component	Cytosol	IDA
GO:0005886	Component	Plasma membrane	IDA
GO:0005925	Component	Focal adhesion	IEA
GO:0005938	Component	Cell cortex	IEA
GO:0007010	Process	Cytoskeleton organization	NAS	11827972
GO:0007049	Process	Cell cycle	IEA
GO:0007155	Process	Cell adhesion	IEA
GO:0007165	Process	Signal transduction	TAS	8668148
GO:0007169	Process	Transmembrane receptor protein tyrosine kinase signaling pathway	IBA	21873635
GO:0007229	Process	Integrin-mediated signaling pathway	NAS	11827972
GO:0016477	Process	Cell migration	IBA	21873635
GO:0030027	Component	Lamellipodium	IEA
GO:0030335	Process	Positive regulation of cell migration	IMP	18256281
GO:0040008	Process	Regulation of growth	IEA
GO:0051017	Process	Actin filament bundle assembly	NAS	11827972
GO:0051301	Process	Cell division	IEA
GO:0061098	Process	Positive regulation of protein tyrosine kinase activity	IDA	18256281
GO:0090527	Process	Actin filament reorganization	IBA	21873635
GO:0090630	Process	Activation of GTPase activity	IEA
GO:1900026	Process	Positive regulation of substrate adhesion-dependent cell spreading	IMP	18256281
GO:1990782	Function	Protein tyrosine kinase binding	IPI	18256281

Other IDs

• MIM: 602265
• HGNC: 7733
• e!Ensembl: ENSG00000111859

Protein

• UniProt ID: Q14511
• Protein name: Enhancer of filamentation 1 (hEF1) (CRK-associated substrate-related protein) (CAS-L) (CasL) (Cas scaffolding protein family member 2) (CASS2) (Neural precursor cell expressed developmentally down-regulated protein 9) (NEDD-9) (Renal carcinoma antigen NY-
• Protein function: Scaffolding protein which plays a central coordinating role for tyrosine-kinase-based signaling related to cell adhesion (PubMed:24574519). As a focal adhesion protein, plays a role in embryonic fibroblast migration (By similarity). May play an
• PDB: 2L81, 5X3S
• Family and domains:

  Pfam

  Accession	ID	Position in sequence	Description	Type
  PF14604	SH3_9	10 → 61	Variant SH3 domain	Domain
  PF08824	Serine_rich	404 → 560	Serine rich protein interaction domain	Domain
  PF12026	CAS_C	619 → 828	Crk-Associated Substrate C-terminal domain	Domain

• Sequence:

  MKYKNLMARALYDNVPECAEELAFRKGDILTVIEQNTGGLEGWWLCSLHGRQGIVPGNRV
  KLLIGPMQETASSHEQPASGLMQQTFGQQKLYQVPNPQAAPRDTIYQVPPSYQNQGIYQV
  PTGHGTQEQEVYQVPPSVQRSIGGTSGPHVGKKVITPVRTGHGYVYEYPSRYQKDVYDIP
  PSHTTQGVYDIPPSSAKGPVFSVPVGEIKPQGVYDIPPTKGVYAIPPSACRDEAGLREKD
  YDFPPPMRQAGRPDLRPEGVYDIPPTCTKPAGKDLHVKYNCDIPGAAEPVARRHQSLSPN
  HPPPQLGQSVGSQNDAYDVPRGVQFLEPPAETSEKANPQERDGVYDVPLHNPPDAKGSRD
  LVDGINRLSFSSTGSTRSNMSTSSTSSKESSLSASPAQDKRLFLDPDTAIERLQRLQQAL
  EMGVSSLMALVTTDWRCYGYMERHINEIRTAVDKVELFLKEYLHFVKGAVANAACLPELI
  LHNKMKRELQRVEDSHQILSQTSHDLNECSWSLNILAINKPQNKCDDLDRFVMVAKTVPD
  DAKQLTTTINTNAEALFRPGPGSLHLKNGPESIMNSTEYPHGGSQGQLLHPGDHKAQAHN
  KALPPGLSKEQAPDCSSSDGSERSWMDDYDYVHLQGKEEFERQQKELLEKENIMKQNKMQ
  LEHHQLSQFQLLEQEITKPVENDISKWKPSQSLPTTNSGVSAQDRQLLCFYYDQCETHFI
  SLLNAIDALFSCVSSAQPPRIFVAHSKFVILSAHKLVFIGDTLTRQVTAQDIRNKVMNSS
  NQLCEQLKTIVMATKMAALHYPSTTALQEMVHQVTDLSRNAQLFKRSLLEMATF

• Sequence length: 834

Associated diseases

Causal
Disease name	Disease term	dbSNP ID	References
Coronary artery disease	Coronary Artery Disease	rs137852988, rs121918313, rs121918529, rs121918531, rs137852340, rs405509, rs1555800701, rs1215189537	29212778
Glaucoma	Glaucoma, Open-Angle	rs121918355, rs1566660365, rs1566635134, rs121918356, rs1566634475, rs28936700, rs55771538, rs28936701, rs104893622, rs55989760, rs72549387, rs104893628, rs2125316417, rs104893629, rs74315328, rs121909193, rs74315330, rs74315329, rs74315332, rs74315334, rs74315336, rs74315338, rs74315341, rs121909194, rs74315331, rs1558603396, rs387907175, rs587778873, rs587778875, rs104894979, rs137854895, rs766425037, rs72549380, rs148542782, rs541217363, rs753021890, rs771076928, rs56010818, rs777678299, rs1446110883, rs1573274915, rs1587545234, rs751768343, rs944452644	22605921
Prostate cancer	Prostate carcinoma	rs121909139, rs121909140, rs121909141, rs121909142, rs121909143, rs606231169, rs606231170, rs137852584, rs137852578, rs137852580, rs137852581, rs137852582	29892016, 25217961