ME1 (malic enzyme 1)

Gene

• Entrez ID: 4199
• Gene name: Malic enzyme 1
• Gene symbol: ME1
• Synonyms: HUMNDME, MES
• Chromosome: 6
• Chromosome location: 6q14.2
• Summary: This gene encodes a cytosolic, NADP-dependent enzyme that generates NADPH for fatty acid biosynthesis. The activity of this enzyme, the reversible oxidative decarboxylation of malate, links the glycolytic and citric acid cycles. The regulation of expressi

miRNA

miRTarBase ID	miRNA	Experiments	Reference
MIRT025454	hsa-miR-34a-5p	Proteomics	21566225
MIRT1139562	hsa-miR-1224-5p	CLIP-seq
MIRT1139563	hsa-miR-1272	CLIP-seq
MIRT1139564	hsa-miR-1276	CLIP-seq
MIRT1139565	hsa-miR-1285	CLIP-seq
MIRT1139566	hsa-miR-1304	CLIP-seq
MIRT1139567	hsa-miR-133a	CLIP-seq
MIRT1139568	hsa-miR-133b	CLIP-seq
MIRT1139569	hsa-miR-144	CLIP-seq
MIRT1139570	hsa-miR-1468	CLIP-seq
MIRT1139571	hsa-miR-2682	CLIP-seq
MIRT1139572	hsa-miR-3117-3p	CLIP-seq
MIRT1139573	hsa-miR-3133	CLIP-seq
MIRT1139574	hsa-miR-3137	CLIP-seq
MIRT1139575	hsa-miR-3163	CLIP-seq
MIRT1139576	hsa-miR-3169	CLIP-seq
MIRT1139577	hsa-miR-3187-5p	CLIP-seq
MIRT1139578	hsa-miR-339-5p	CLIP-seq
MIRT1139579	hsa-miR-3591-5p	CLIP-seq
MIRT1139580	hsa-miR-3605-3p	CLIP-seq
MIRT1139581	hsa-miR-3662	CLIP-seq
MIRT1139582	hsa-miR-374c	CLIP-seq
MIRT1139583	hsa-miR-378g	CLIP-seq
MIRT1139584	hsa-miR-3915	CLIP-seq
MIRT1139585	hsa-miR-3925-5p	CLIP-seq
MIRT1139586	hsa-miR-3928	CLIP-seq
MIRT1139587	hsa-miR-3945	CLIP-seq
MIRT1139588	hsa-miR-4253	CLIP-seq
MIRT1139589	hsa-miR-4263	CLIP-seq
MIRT1139590	hsa-miR-4311	CLIP-seq
MIRT1139591	hsa-miR-4457	CLIP-seq
MIRT1139592	hsa-miR-4486	CLIP-seq
MIRT1139593	hsa-miR-4496	CLIP-seq
MIRT1139594	hsa-miR-449c	CLIP-seq
MIRT1139595	hsa-miR-4509	CLIP-seq
MIRT1139596	hsa-miR-4515	CLIP-seq
MIRT1139597	hsa-miR-4521	CLIP-seq
MIRT1139598	hsa-miR-4668-3p	CLIP-seq
MIRT1139599	hsa-miR-4672	CLIP-seq
MIRT1139600	hsa-miR-4689	CLIP-seq
MIRT1139601	hsa-miR-4709-5p	CLIP-seq
MIRT1139602	hsa-miR-4744	CLIP-seq
MIRT1139603	hsa-miR-4759	CLIP-seq
MIRT1139604	hsa-miR-4775	CLIP-seq
MIRT1139605	hsa-miR-4799-5p	CLIP-seq
MIRT1139606	hsa-miR-486-5p	CLIP-seq
MIRT1139607	hsa-miR-524-3p	CLIP-seq
MIRT1139608	hsa-miR-525-3p	CLIP-seq
MIRT1139609	hsa-miR-548a-5p	CLIP-seq
MIRT1139610	hsa-miR-548ab	CLIP-seq
MIRT1139611	hsa-miR-548ak	CLIP-seq
MIRT1139612	hsa-miR-548b-5p	CLIP-seq
MIRT1139613	hsa-miR-548c-3p	CLIP-seq
MIRT1139614	hsa-miR-548c-5p	CLIP-seq
MIRT1139615	hsa-miR-548d-5p	CLIP-seq
MIRT1139616	hsa-miR-548h	CLIP-seq
MIRT1139617	hsa-miR-548i	CLIP-seq
MIRT1139618	hsa-miR-548j	CLIP-seq
MIRT1139619	hsa-miR-548k	CLIP-seq
MIRT1139620	hsa-miR-548l	CLIP-seq
MIRT1139621	hsa-miR-548w	CLIP-seq
MIRT1139622	hsa-miR-548y	CLIP-seq
MIRT1139623	hsa-miR-559	CLIP-seq
MIRT1139624	hsa-miR-568	CLIP-seq
MIRT1139625	hsa-miR-576-5p	CLIP-seq
MIRT1139626	hsa-miR-583	CLIP-seq
MIRT1139627	hsa-miR-590-3p	CLIP-seq
MIRT1139628	hsa-miR-612	CLIP-seq
MIRT1139629	hsa-miR-622	CLIP-seq
MIRT1139630	hsa-miR-655	CLIP-seq
MIRT1139631	hsa-miR-940	CLIP-seq
MIRT2039488	hsa-miR-125a-3p	CLIP-seq
MIRT2039489	hsa-miR-3167	CLIP-seq
MIRT2039490	hsa-miR-345	CLIP-seq
MIRT2039491	hsa-miR-3609	CLIP-seq
MIRT2039492	hsa-miR-3934	CLIP-seq
MIRT2039493	hsa-miR-4256	CLIP-seq
MIRT2039494	hsa-miR-4654	CLIP-seq
MIRT2039495	hsa-miR-4733-5p	CLIP-seq
MIRT2039496	hsa-miR-4762-3p	CLIP-seq
MIRT2039497	hsa-miR-4769-5p	CLIP-seq
MIRT2039498	hsa-miR-520g	CLIP-seq
MIRT2039499	hsa-miR-520h	CLIP-seq
MIRT2039500	hsa-miR-548ah	CLIP-seq
MIRT2039501	hsa-miR-548p	CLIP-seq
MIRT2039502	hsa-miR-764	CLIP-seq
MIRT2039503	hsa-miR-767-3p	CLIP-seq
MIRT2039504	hsa-miR-876-5p	CLIP-seq
MIRT2269454	hsa-miR-137	CLIP-seq
MIRT2269455	hsa-miR-25	CLIP-seq
MIRT2269456	hsa-miR-32	CLIP-seq
MIRT2039491	hsa-miR-3609	CLIP-seq
MIRT2269457	hsa-miR-363	CLIP-seq
MIRT2269458	hsa-miR-367	CLIP-seq
MIRT2269459	hsa-miR-374a	CLIP-seq
MIRT2269460	hsa-miR-374b	CLIP-seq
MIRT2269461	hsa-miR-4269	CLIP-seq
MIRT2269462	hsa-miR-4514	CLIP-seq
MIRT2269463	hsa-miR-4692	CLIP-seq
MIRT2269464	hsa-miR-4742-5p	CLIP-seq
MIRT2039498	hsa-miR-520g	CLIP-seq
MIRT2039499	hsa-miR-520h	CLIP-seq
MIRT2039500	hsa-miR-548ah	CLIP-seq
MIRT2269465	hsa-miR-92a	CLIP-seq
MIRT2269466	hsa-miR-92b	CLIP-seq
MIRT1139562	hsa-miR-1224-5p	CLIP-seq
MIRT2568747	hsa-miR-1245	CLIP-seq
MIRT2568748	hsa-miR-1273f	CLIP-seq
MIRT1139566	hsa-miR-1304	CLIP-seq
MIRT2568749	hsa-miR-143	CLIP-seq
MIRT2568750	hsa-miR-186	CLIP-seq
MIRT2568751	hsa-miR-30a	CLIP-seq
MIRT2568752	hsa-miR-30b	CLIP-seq
MIRT2568753	hsa-miR-30c	CLIP-seq
MIRT2568754	hsa-miR-30d	CLIP-seq
MIRT2568755	hsa-miR-30e	CLIP-seq
MIRT2568756	hsa-miR-3607-3p	CLIP-seq
MIRT2568757	hsa-miR-3607-5p	CLIP-seq
MIRT2039491	hsa-miR-3609	CLIP-seq
MIRT2568758	hsa-miR-3664-3p	CLIP-seq
MIRT1139584	hsa-miR-3915	CLIP-seq
MIRT2568759	hsa-miR-3920	CLIP-seq
MIRT1139586	hsa-miR-3928	CLIP-seq
MIRT1139591	hsa-miR-4457	CLIP-seq
MIRT1139593	hsa-miR-4496	CLIP-seq
MIRT2568760	hsa-miR-4519	CLIP-seq
MIRT1139597	hsa-miR-4521	CLIP-seq
MIRT2568761	hsa-miR-4659a-3p	CLIP-seq
MIRT2568762	hsa-miR-4659b-3p	CLIP-seq
MIRT1139598	hsa-miR-4668-3p	CLIP-seq
MIRT1139600	hsa-miR-4689	CLIP-seq
MIRT2568763	hsa-miR-4708-5p	CLIP-seq
MIRT2568764	hsa-miR-4712-3p	CLIP-seq
MIRT2568765	hsa-miR-4724-3p	CLIP-seq
MIRT2039495	hsa-miR-4733-5p	CLIP-seq
MIRT2568766	hsa-miR-4753-3p	CLIP-seq
MIRT2568767	hsa-miR-4761-5p	CLIP-seq
MIRT2039496	hsa-miR-4762-3p	CLIP-seq
MIRT2568768	hsa-miR-4768-5p	CLIP-seq
MIRT2568769	hsa-miR-4770	CLIP-seq
MIRT2568770	hsa-miR-488	CLIP-seq
MIRT2568771	hsa-miR-510	CLIP-seq
MIRT2568772	hsa-miR-515-5p	CLIP-seq
MIRT2039498	hsa-miR-520g	CLIP-seq
MIRT2039499	hsa-miR-520h	CLIP-seq
MIRT1139607	hsa-miR-524-3p	CLIP-seq
MIRT1139608	hsa-miR-525-3p	CLIP-seq
MIRT2568773	hsa-miR-548ag	CLIP-seq
MIRT2039500	hsa-miR-548ah	CLIP-seq
MIRT2568774	hsa-miR-548ai	CLIP-seq
MIRT2039501	hsa-miR-548p	CLIP-seq
MIRT2568775	hsa-miR-548u	CLIP-seq
MIRT2568776	hsa-miR-942	CLIP-seq
MIRT2568748	hsa-miR-1273f	CLIP-seq
MIRT2568749	hsa-miR-143	CLIP-seq
MIRT2568750	hsa-miR-186	CLIP-seq
MIRT2684773	hsa-miR-194	CLIP-seq
MIRT2568751	hsa-miR-30a	CLIP-seq
MIRT2568752	hsa-miR-30b	CLIP-seq
MIRT2568753	hsa-miR-30c	CLIP-seq
MIRT2568754	hsa-miR-30d	CLIP-seq
MIRT2568755	hsa-miR-30e	CLIP-seq
MIRT2568757	hsa-miR-3607-5p	CLIP-seq
MIRT2568758	hsa-miR-3664-3p	CLIP-seq
MIRT1139591	hsa-miR-4457	CLIP-seq
MIRT1139597	hsa-miR-4521	CLIP-seq
MIRT1139598	hsa-miR-4668-3p	CLIP-seq
MIRT2568763	hsa-miR-4708-5p	CLIP-seq
MIRT2568764	hsa-miR-4712-3p	CLIP-seq
MIRT2568767	hsa-miR-4761-5p	CLIP-seq
MIRT2568769	hsa-miR-4770	CLIP-seq
MIRT2568770	hsa-miR-488	CLIP-seq
MIRT2568771	hsa-miR-510	CLIP-seq
MIRT2568773	hsa-miR-548ag	CLIP-seq
MIRT2568774	hsa-miR-548ai	CLIP-seq
MIRT2039501	hsa-miR-548p	CLIP-seq
MIRT2568775	hsa-miR-548u	CLIP-seq

Transcription factors

Transcription factor	Regulation	Reference
SP1	Unknown	9872397

Gene ontology (GO)

GO ID	Ontology	Definition	Evidence	Reference
GO:0004470	Function	Malic enzyme activity	IBA	21873635
GO:0004470	Function	Malic enzyme activity	IDA	7622060, 8187880
GO:0004470	Function	Malic enzyme activity	IMP	23334421
GO:0004471	Function	Malate dehydrogenase (decarboxylating) (NAD+) activity	IEA
GO:0004473	Function	Malate dehydrogenase (decarboxylating) (NADP+) activity	IBA	21873635
GO:0004473	Function	Malate dehydrogenase (decarboxylating) (NADP+) activity	IDA	8187880
GO:0004473	Function	Malate dehydrogenase (decarboxylating) (NADP+) activity	ISS
GO:0005739	Component	Mitochondrion	IBA	21873635
GO:0005829	Component	Cytosol	ISS
GO:0005829	Component	Cytosol	TAS
GO:0005975	Process	Carbohydrate metabolic process	NAS	8187880
GO:0006090	Process	Pyruvate metabolic process	IBA	21873635
GO:0006090	Process	Pyruvate metabolic process	TAS
GO:0006108	Process	Malate metabolic process	IBA	21873635
GO:0006108	Process	Malate metabolic process	IDA	7622060, 8187880
GO:0008948	Function	Oxaloacetate decarboxylase activity	IEA
GO:0009055	Function	Electron transfer activity	TAS	7622060
GO:0009165	Process	Nucleotide biosynthetic process	TAS	8187880
GO:0009725	Process	Response to hormone	ISS
GO:0009743	Process	Response to carbohydrate	TAS	8187880
GO:0019216	Process	Regulation of lipid metabolic process	TAS
GO:0022900	Process	Electron transport chain	IEA
GO:0030145	Function	Manganese ion binding	IDA	7622060
GO:0042802	Function	Identical protein binding	IPI	32296183
GO:0043531	Function	ADP binding	TAS	7622060
GO:0050661	Function	NADP binding	TAS	7622060
GO:0051287	Function	NAD binding	TAS	7622060
GO:1902031	Process	Regulation of NADP metabolic process	IMP	23334421

Other IDs

• MIM: 154250
• HGNC: 6983
• e!Ensembl: ENSG00000065833

Protein

• UniProt ID: P48163
• Protein name: NADP-dependent malic enzyme (NADP-ME) (EC 1.1.1.40) (Malic enzyme 1)
• Protein function: Catalyzes the oxidative decarboxylation of (S)-malate in the presence of NADP(+) and divalent metal ions, and decarboxylation of oxaloacetate. {ECO:0000269|PubMed:7622060, ECO:0000269|PubMed:7757881, ECO:0000269|PubMed:8187880, ECO:0000269|PubMe
• PDB: 2AW5, 3WJA, 7X11, 7X12
• Family and domains:

  Pfam

  Accession	ID	Position in sequence	Description	Type
  PF00390	malic	79 → 260	Malic enzyme, N-terminal domain	Domain
  PF03949	Malic_M	270 → 522	Malic enzyme, NAD binding domain	Domain

• Sequence:

  MEPEAPRRRHTHQRGYLLTRNPHLNKDLAFTLEERQQLNIHGLLPPSFNSQEIQVLRVVK
  NFEHLNSDFDRYLLLMDLQDRNEKLFYRVLTSDIEKFMPIVYTPTVGLACQQYSLVFRKP
  RGLFITIHDRGHIASVLNAWPEDVIKAIVVTDGERILGLGDLGCNGMGIPVGKLALYTAC
  GGMNPQECLPVILDVGTENEELLKDPLYIGLRQRRVRGSEYDDFLDEFMEAVSSKYGMNC
  LIQFEDFANVNAFRLLNKYRNQYCTFNDDIQGTASVAVAGLLAALRITKNKLSDQTILFQ
  GAGEAALGIAHLIVMALEKEGLPKEKAIKKIWLVDSKGLIVKGRASLTQEKEKFAHEHEE
  MKNLEAIVQEIKPTALIGVAAIGGAFSEQILKDMAAFNERPIIFALSNPTSKAECSAEQC
  YKITKGRAIFASGSPFDPVTLPNGQTLYPGQGNNSYVFPGVALGVVACGLRQITDNIFLT
  TAEVIAQQVSDKHLEEGRLYPPLNTIRDVSLKIAEKIVKDAYQEKTATVYPEPQNKEAFV
  RSQMYSTDYDQILPDCYSWPEEVQKIQTKVDQ

• Sequence length: 572

Pathways

KEGG:

Pyruvate metabolism
Metabolic pathways
Carbon metabolism
PPAR signaling pathway

Reactome:

PPARA activates gene expression
Pyruvate metabolism

Associated diseases

Causal
Disease name	Disease term	dbSNP ID	References
Obesity	Obesity	rs34911341, rs74315349, rs1474810899, rs2282440, rs2491132, rs121918111, rs796065034, rs753856820, rs796065035, rs121918112, rs104894023, rs137852821, rs1580764441, rs137852822, rs137852823, rs137852824, rs13447324, rs121913562, rs121913564, rs74315393, rs121913556, rs2989924, rs193922650, rs193922685, rs193922687, rs751160202, rs1421085, rs747681609, rs1553400259, rs13447339, rs370479598, rs1554394014, rs1553174844, rs756232889, rs369841551, rs1557670950, rs1571321748, rs148538980, rs1572820988, rs1591461970, rs1419374563, rs745921568, rs144159890, rs1570714352, rs779783209, rs1573250294, rs1573254045, rs1580744791, rs1580746829, rs6548238, rs7138803, rs7754840	19270708

Unknown
Disease name	Disease term	dbSNP ID	References
Liver carcinoma	Liver carcinoma		21147110