MCM6 (minichromosome maintenance complex component 6)

Gene

• Entrez ID: 4175
• Gene name: Minichromosome maintenance complex component 6
• Gene symbol: MCM6
• Synonyms: MCG40308, Mis5, P105MCM
• Chromosome: 2
• Chromosome location: 2q21.3
• Summary: The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component

miRNA

miRTarBase ID	miRNA	Experiments	Reference
MIRT016611	hsa-miR-193b-3p	Microarray	20304954
MIRT016611	hsa-miR-193b-3p	Proteomics	21512034
MIRT024027	hsa-miR-1-3p	Proteomics	18668040
MIRT024879	hsa-miR-215-5p	Microarray	19074876
MIRT025545	hsa-miR-34a-5p	Proteomics	21566225
MIRT026808	hsa-miR-192-5p	Microarray	19074876
MIRT035931	hsa-miR-1180-3p	CLASH	23622248
MIRT1137756	hsa-miR-1178	CLIP-seq
MIRT1137757	hsa-miR-1197	CLIP-seq
MIRT1137758	hsa-miR-1302	CLIP-seq
MIRT1137759	hsa-miR-3128	CLIP-seq
MIRT1137760	hsa-miR-329	CLIP-seq
MIRT1137761	hsa-miR-3619-3p	CLIP-seq
MIRT1137762	hsa-miR-362-3p	CLIP-seq
MIRT1137763	hsa-miR-369-3p	CLIP-seq
MIRT1137764	hsa-miR-374a	CLIP-seq
MIRT1137765	hsa-miR-374b	CLIP-seq
MIRT1137766	hsa-miR-4261	CLIP-seq
MIRT1137767	hsa-miR-4298	CLIP-seq
MIRT1137768	hsa-miR-4420	CLIP-seq
MIRT1137769	hsa-miR-4496	CLIP-seq
MIRT1137770	hsa-miR-4679	CLIP-seq
MIRT1137771	hsa-miR-4775	CLIP-seq
MIRT1137772	hsa-miR-4786-3p	CLIP-seq
MIRT1137773	hsa-miR-4789-3p	CLIP-seq
MIRT1137774	hsa-miR-487a	CLIP-seq
MIRT1137775	hsa-miR-548l	CLIP-seq
MIRT1137776	hsa-miR-548n	CLIP-seq
MIRT1137777	hsa-miR-548t	CLIP-seq
MIRT1137778	hsa-miR-553	CLIP-seq
MIRT1137779	hsa-miR-590-3p	CLIP-seq
MIRT1137780	hsa-miR-642b	CLIP-seq
MIRT1137781	hsa-miR-649	CLIP-seq
MIRT1137756	hsa-miR-1178	CLIP-seq
MIRT1137757	hsa-miR-1197	CLIP-seq
MIRT1137773	hsa-miR-4789-3p	CLIP-seq
MIRT2039120	hsa-miR-570	CLIP-seq
MIRT2269164	hsa-miR-149	CLIP-seq
MIRT2269165	hsa-miR-3064-5p	CLIP-seq
MIRT2269166	hsa-miR-4794	CLIP-seq
MIRT2269167	hsa-miR-604	CLIP-seq
MIRT2269168	hsa-miR-647	CLIP-seq
MIRT2269169	hsa-miR-767-3p	CLIP-seq
MIRT2568407	hsa-miR-3180-5p	CLIP-seq
MIRT2568408	hsa-miR-3182	CLIP-seq
MIRT1137761	hsa-miR-3619-3p	CLIP-seq
MIRT2568409	hsa-miR-3690	CLIP-seq
MIRT2568410	hsa-miR-4254	CLIP-seq
MIRT2568411	hsa-miR-4643	CLIP-seq
MIRT2568412	hsa-miR-4762-3p	CLIP-seq
MIRT2568413	hsa-miR-4766-5p	CLIP-seq
MIRT1137773	hsa-miR-4789-3p	CLIP-seq
MIRT2568414	hsa-miR-520a-5p	CLIP-seq
MIRT2568415	hsa-miR-525-5p	CLIP-seq
MIRT2039120	hsa-miR-570	CLIP-seq
MIRT2568416	hsa-miR-578	CLIP-seq
MIRT1137780	hsa-miR-642b	CLIP-seq
MIRT2568417	hsa-miR-671-5p	CLIP-seq
MIRT2568418	hsa-miR-766	CLIP-seq
MIRT1137761	hsa-miR-3619-3p	CLIP-seq
MIRT2568409	hsa-miR-3690	CLIP-seq
MIRT2568410	hsa-miR-4254	CLIP-seq
MIRT2568412	hsa-miR-4762-3p	CLIP-seq
MIRT2568413	hsa-miR-4766-5p	CLIP-seq
MIRT1137773	hsa-miR-4789-3p	CLIP-seq
MIRT2039120	hsa-miR-570	CLIP-seq
MIRT2568416	hsa-miR-578	CLIP-seq
MIRT1137780	hsa-miR-642b	CLIP-seq
MIRT2568417	hsa-miR-671-5p	CLIP-seq

Transcription factors

Transcription factor	Regulation	Reference
MYCN	Activation	17826980

Gene ontology (GO)

GO ID	Ontology	Definition	Evidence	Reference
GO:0000082	Process	G1/S transition of mitotic cell cycle	TAS
GO:0000727	Process	Double-strand break repair via break-induced replication	IBA	21873635
GO:0000781	Component	Chromosome, telomeric region	HDA	19135898
GO:0003678	Function	DNA helicase activity	IDA	9305914
GO:0003688	Function	DNA replication origin binding	IBA	21873635
GO:0003697	Function	Single-stranded DNA binding	IBA	21873635
GO:0005515	Function	Protein binding	IPI	11095689, 15232106, 16189514, 16438930, 16899510, 16902406, 17296731, 17955473, 20202939, 22190034, 22540012, 23764002, 24299456, 25036637, 25416956, 26167883, 26496610, 28514442, 29290612, 29426014
GO:0005524	Function	ATP binding	NAS	9286856
GO:0005634	Component	Nucleus	IBA	21873635
GO:0005634	Component	Nucleus	IDA	16899510
GO:0005634	Component	Nucleus	NAS	9286856
GO:0005654	Component	Nucleoplasm	IDA
GO:0005654	Component	Nucleoplasm	TAS
GO:0006260	Process	DNA replication	NAS	9286856
GO:0006260	Process	DNA replication	TAS
GO:0006267	Process	Pre-replicative complex assembly involved in nuclear cell cycle DNA replication	IBA	21873635
GO:0006268	Process	DNA unwinding involved in DNA replication	IBA	21873635
GO:0006270	Process	DNA replication initiation	IEA
GO:0042555	Component	MCM complex	IBA	21873635
GO:0042555	Component	MCM complex	IDA	17296731
GO:0042802	Function	Identical protein binding	IPI	15232106, 16189514
GO:0071162	Component	CMG complex	ISS
GO:1902969	Process	Mitotic DNA replication	IBA	21873635
GO:1990518	Function	Single-stranded 3'-5' DNA helicase activity	IBA	21873635

Other IDs

• MIM: 601806
• HGNC: 6949
• e!Ensembl: ENSG00000076003

Protein

• UniProt ID: Q14566
• Protein name: DNA replication licensing factor MCM6 (EC 3.6.4.12) (p105MCM)
• Protein function: Acts as a component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. Core component of CDC45-MCM-GINS (CMG) helicase, the mol
• PDB: 2KLQ, 2LE8, 6XTX, 6XTY, 7PFO, 7PLO, 7W1Y, 7W68, 8B9D, 8RWV, 8S09, 8S0A, 8S0B, 8S0D, 8S0E, 8S0F, 8W0E, 8W0F, 8W0G, 8W0I, 9CAQ
• Family and domains:

  Pfam

  Accession	ID	Position in sequence	Description	Type
  PF14551	MCM_N	27 → 127	MCM N-terminal domain	Domain
  PF17207	MCM_OB	122 → 252	MCM OB domain	Domain
  PF00493	MCM	333 → 556	MCM P-loop domain	Domain
  PF17855	MCM_lid	570 → 656	MCM AAA-lid domain	Domain
  PF18263	MCM6_C	711 → 819	MCM6 C-terminal winged-helix domain	Domain

• Sequence:

  MDLAAAAEPGAGSQHLEVRDEVAEKCQKLFLDFLEEFQSSDGEIKYLQLAEELIRPERNT
  LVVSFVDLEQFNQQLSTTIQEEFYRVYPYLCRALKTFVKDRKEIPLAKDFYVAFQDLPTR
  HKIRELTSSRIGLLTRISGQVVRTHPVHPELVSGTFLCLDCQTVIRDVEQQFKYTQPNIC
  RNPVCANRRRFLLDTNKSRFVDFQKVRIQETQAELPRGSIPRSLEVILRAEAVESAQAGD
  KCDFTGTLIVVPDVSKLSTPGARAETNSRVSGVDGYETEGIRGLRALGVRDLSYRLVFLA
  CCVAPTNPRFGGKELRDEEQTAESIKNQMTVKEWEKVFEMSQDKNLYHNLCTSLFPTIHG
  NDEVKRGVLLMLFGGVPKTTGEGTSLRGDINVCIVGDPSTAKSQFLKHVEEFSPRAVYTS
  GKASSAAGLTAAVVRDEESHEFVIEAGALMLADNGVCCIDEFDKMDVRDQVAIHEAMEQQ
  TISITKAGVKATLNARTSILAAANPISGHYDRSKSLKQNINLSAPIMSRFDLFFILVDEC
  NEVTDYAIARRIVDLHSRIEESIDRVYSLDDIRRYLLFARQFKPKISKESEDFIVEQYKH
  LRQRDGSGVTKSSWRITVRQLESMIRLSEAMARMHCCDEVQPKHVKEAFRLLNKSIIRVE
  TPDVNLDQEEEIQMEVDEGAGGINGHADSPAPVNGINGYNEDINQESAPKASLRLGFSEY
  CRISNLIVLHLRKVEEEEDESALKRSELVNWYLKEIESEIDSEEELINKKRIIEKVIHRL
  THYDHVLIELTQAGLKGSTEGSESYEEDPYLVVNPNYLLED

• Sequence length: 821

Pathways

KEGG:

DNA replication
Cell cycle

Reactome:

Activation of ATR in response to replication stress
Assembly of the pre-replicative complex
Orc1 removal from chromatin
Activation of the pre-replicative complex
Switching of origins to a post-replicative state

Associated diseases

Causal
Disease name	Disease term	dbSNP ID	References
Diabetes mellitus	Diabetes Mellitus, Non-Insulin-Dependent	rs587776515, rs61730328, rs606231121, rs606231122, rs79020217, rs77625743, rs78378398, rs606231123, rs4402960, rs1362648752, rs3745368, rs3792267, rs3842570, rs5030952, rs2975760, rs119489103, rs7903146, rs12255372, rs11196205, rs104893649, rs80356624, rs80356616, rs80356625, rs80356611, rs104894237, rs80356613, rs137852740, rs137852786, rs387906407, rs151344623, rs28938469, rs28936371, rs137852672, rs80356637, rs80356642, rs80356653, rs137852673, rs137852674, rs80356634, rs80356651, rs193929360, rs137853334, rs137853335, rs137853336, rs1600731198, rs137853338, rs121964882, rs121964883, rs387906511, rs121964884, rs121964885, rs2147483647, rs387906512, rs121964887, rs121964888, rs121964889, rs121964890, rs121964891, rs28934878, rs74315383, rs121964893, rs886037620, rs886037621, rs80356663, rs121434593, rs121913150, rs587776825, rs137853236, rs2135842335, rs137853237, rs137853238, rs2135818776, rs1566092470, rs1463923467, rs137853243, rs137853244, rs2135839114, rs137853245, rs2135847417, rs121918407, rs104894005, rs104894006, rs80356655, rs104894008, rs104894009, rs104894010, rs104894011, rs80356654, rs104894016, rs193929376, rs193929374, rs193929375, rs193929373, rs80356666, rs80356669, rs80356664, rs193929366, rs1048095, rs193929355, rs193929356, rs1259467443, rs104893642, rs387906777, rs387906779, rs141804752, rs182349376, rs184917682, rs193922396, rs193922400, rs193922401, rs137852676, rs193922407, rs193922638, rs193922257, rs193922258, rs193922259, rs193922260, rs193922261, rs193922262, rs193922263, rs193922264, rs193922265, rs193922268, rs193921338, rs193922269, rs193922272, rs193922273, rs193922275, rs193922278, rs193922279, rs193922280, rs193922281, rs193922282, rs193922283, rs193922284, rs193922286, rs193922287, rs193922289, rs193922291, rs193922295, rs193922297, rs193922300, rs193922302, rs193922303, rs193922308, rs193922313, rs193922314, rs144723656, rs193922315, rs193922316, rs193922317, rs148311934, rs193922319, rs193922320, rs193922326, rs193922329, rs193922330, rs193922331, rs193922335, rs193922336, rs193922338, rs193922340, rs193922341, rs193922471, rs193922475, rs193922476, rs193922479, rs193922355, rs193922356, rs193922576, rs193922578, rs193922582, rs193922588, rs193922592, rs193922594, rs193922596, rs386134267, rs193922598, rs193922599, rs193922600, rs193922604, rs193922605, rs397514580, rs397515519, rs267601516, rs587780343, rs587780345, rs587780346, rs587780347, rs587780357, rs148954387, rs61736969, rs587783673, rs587783672, rs587783669, rs786204676, rs794727236, rs151344624, rs794727775, rs794727839, rs199946797, rs869320673, rs796065047, rs759072800, rs797045595, rs797045209, rs797045207, rs797045213, rs797045623, rs863225280, rs149703259, rs864321656, rs139964066, rs777870079, rs878853246, rs769268803, rs886039380, rs886041392, rs886041391, rs886042610, rs143064649, rs1057516192, rs746480424, rs1057516281, rs576684889, rs754728827, rs1057520291, rs1057520779, rs893256143, rs1057520504, rs1057524790, rs1057524902, rs1057524904, rs1057524905, rs764232985, rs1064793998, rs1064794268, rs769086289, rs369429452, rs1085307913, rs1131691416, rs765432081, rs1131692182, rs748749585, rs1554335441, rs762263694, rs1312678560, rs767565869, rs1375656631, rs1554335391, rs1360415315, rs1554335616, rs1554335752, rs1554909277, rs769518471, rs757171524, rs768951263, rs762703502, rs1555212248, rs1555212359, rs1555813319, rs1555816654, rs1553638903, rs1553638909, rs948820149, rs371977235, rs1553784995, rs76474829, rs200998587, rs1415041911, rs1554334894, rs1260178539, rs1554335421, rs1555211904, rs779184183, rs1554335564, rs200670692, rs1400535021, rs1554334872, rs1555212749, rs1553876668, rs1553878211, rs954727530, rs1554924035, rs372307320, rs925231098, rs1554913069, rs1554933565, rs766431403, rs746714109, rs751279984, rs770664202, rs1008906426, rs758844607, rs1554924540, rs1566092307, rs753998395, rs1565885935, rs1167124132, rs1376796469, rs556436603, rs1562715657, rs1486280029, rs1564869850, rs755259997, rs769569410, rs1172328722, rs1286294151, rs1375557127, rs1568731279, rs1562715426, rs556581174, rs1564865302, rs1565886545, rs776793516, rs1568724014, rs1392795567, rs781260712, rs1562719705, rs1382448285, rs1564977373, rs750586210, rs1598842892, rs1583592247, rs780612692, rs1593060859, rs1476637197, rs751279776, rs1593060890, rs1191912908, rs1167675604, rs1583601110, rs1593058932, rs778611627, rs753296261	28736931

Unknown
Disease name	Disease term	dbSNP ID	References
Lactase non-persistence	NON RARE IN EUROPE: Lactase non-persistence in adulthood
Lactose intolerance	Lactose Intolerance, Lactose Intolerance, Adult Type