ID3 (inhibitor of DNA binding 3)

Gene

• Entrez ID: 3399
• Gene name: Inhibitor of DNA binding 3
• Gene symbol: ID3
• Synonyms: HEIR-1, bHLHb25
• Chromosome: 1
• Chromosome location: 1p36.12
• Summary: The protein encoded by this gene is a helix-loop-helix (HLH) protein that can form heterodimers with other HLH proteins. However, the encoded protein lacks a basic DNA-binding domain and therefore inhibits the DNA binding of any HLH protein with which it

miRNA

miRTarBase ID	miRNA	Experiments	Reference
MIRT004740	hsa-miR-520h	Microarray, qRT-PCR	19435428
MIRT022266	hsa-miR-124-3p	Microarray	18668037
MIRT657245	hsa-miR-634	HITS-CLIP	23824327
MIRT657244	hsa-miR-7151-5p	HITS-CLIP	23824327
MIRT657243	hsa-miR-205-3p	HITS-CLIP	23824327
MIRT657242	hsa-miR-4762-5p	HITS-CLIP	23824327
MIRT657241	hsa-miR-338-3p	HITS-CLIP	23824327
MIRT455442	hsa-miR-4476	PAR-CLIP	23592263
MIRT455441	hsa-miR-6876-5p	PAR-CLIP	23592263
MIRT455440	hsa-miR-5002-5p	PAR-CLIP	23592263
MIRT455439	hsa-miR-6513-3p	PAR-CLIP	23592263
MIRT455438	hsa-miR-665	PAR-CLIP	23592263
MIRT455437	hsa-miR-766-3p	PAR-CLIP	23592263
MIRT455436	hsa-miR-3925-3p	PAR-CLIP	23592263
MIRT455434	hsa-miR-7515	PAR-CLIP	23592263
MIRT455435	hsa-miR-6132	PAR-CLIP	23592263
MIRT455433	hsa-miR-6836-5p	PAR-CLIP	23592263
MIRT455432	hsa-miR-3202	PAR-CLIP	23592263
MIRT455431	hsa-miR-1207-5p	PAR-CLIP	23592263
MIRT455430	hsa-miR-4763-3p	PAR-CLIP	23592263
MIRT455429	hsa-miR-6721-5p	PAR-CLIP	23592263
MIRT455428	hsa-miR-4474-5p	PAR-CLIP	23592263
MIRT455427	hsa-miR-7855-5p	PAR-CLIP	23592263
MIRT455436	hsa-miR-3925-3p	PAR-CLIP	26701625
MIRT455428	hsa-miR-4474-5p	PAR-CLIP	26701625
MIRT455437	hsa-miR-766-3p	PAR-CLIP	26701625
MIRT657245	hsa-miR-634	HITS-CLIP	23824327
MIRT657244	hsa-miR-7151-5p	HITS-CLIP	23824327
MIRT657243	hsa-miR-205-3p	HITS-CLIP	23824327
MIRT657242	hsa-miR-4762-5p	HITS-CLIP	23824327
MIRT657241	hsa-miR-338-3p	HITS-CLIP	23824327
MIRT455442	hsa-miR-4476	PAR-CLIP	23592263
MIRT455441	hsa-miR-6876-5p	PAR-CLIP	23592263
MIRT455440	hsa-miR-5002-5p	PAR-CLIP	23592263
MIRT455439	hsa-miR-6513-3p	PAR-CLIP	23592263
MIRT455438	hsa-miR-665	PAR-CLIP	23592263
MIRT455437	hsa-miR-766-3p	PAR-CLIP	23592263
MIRT455436	hsa-miR-3925-3p	PAR-CLIP	23592263
MIRT455434	hsa-miR-7515	PAR-CLIP	23592263
MIRT455435	hsa-miR-6132	PAR-CLIP	23592263
MIRT455433	hsa-miR-6836-5p	PAR-CLIP	23592263
MIRT455432	hsa-miR-3202	PAR-CLIP	23592263
MIRT455431	hsa-miR-1207-5p	PAR-CLIP	23592263
MIRT455430	hsa-miR-4763-3p	PAR-CLIP	23592263
MIRT455429	hsa-miR-6721-5p	PAR-CLIP	23592263
MIRT455428	hsa-miR-4474-5p	PAR-CLIP	23592263
MIRT455427	hsa-miR-7855-5p	PAR-CLIP	23592263
MIRT1059008	hsa-miR-1180	CLIP-seq
MIRT1059009	hsa-miR-1228	CLIP-seq
MIRT1059010	hsa-miR-1270	CLIP-seq
MIRT1059011	hsa-miR-1275	CLIP-seq
MIRT1059012	hsa-miR-128	CLIP-seq
MIRT1059013	hsa-miR-185	CLIP-seq
MIRT1059014	hsa-miR-1910	CLIP-seq
MIRT1059015	hsa-miR-1972	CLIP-seq
MIRT1059016	hsa-miR-224	CLIP-seq
MIRT1059017	hsa-miR-2278	CLIP-seq
MIRT1059018	hsa-miR-27a	CLIP-seq
MIRT1059019	hsa-miR-27b	CLIP-seq
MIRT1059020	hsa-miR-29a	CLIP-seq
MIRT1059021	hsa-miR-29b	CLIP-seq
MIRT1059022	hsa-miR-29c	CLIP-seq
MIRT1059023	hsa-miR-3127-5p	CLIP-seq
MIRT1059024	hsa-miR-3163	CLIP-seq
MIRT1059025	hsa-miR-340	CLIP-seq
MIRT1059026	hsa-miR-342-5p	CLIP-seq
MIRT1059027	hsa-miR-3667-3p	CLIP-seq
MIRT1059028	hsa-miR-3692	CLIP-seq
MIRT1059029	hsa-miR-3918	CLIP-seq
MIRT1059030	hsa-miR-4259	CLIP-seq
MIRT1059031	hsa-miR-4260	CLIP-seq
MIRT1059032	hsa-miR-4300	CLIP-seq
MIRT1059033	hsa-miR-4306	CLIP-seq
MIRT1059034	hsa-miR-4531	CLIP-seq
MIRT1059035	hsa-miR-4532	CLIP-seq
MIRT1059036	hsa-miR-4644	CLIP-seq
MIRT1059037	hsa-miR-4650-5p	CLIP-seq
MIRT1059038	hsa-miR-4651	CLIP-seq
MIRT1059039	hsa-miR-4664-5p	CLIP-seq
MIRT1059040	hsa-miR-4665-5p	CLIP-seq
MIRT1059041	hsa-miR-4691-3p	CLIP-seq
MIRT1059042	hsa-miR-4709-3p	CLIP-seq
MIRT1059043	hsa-miR-4717-5p	CLIP-seq
MIRT1059044	hsa-miR-4722-5p	CLIP-seq
MIRT1059045	hsa-miR-4768-3p	CLIP-seq
MIRT1059046	hsa-miR-483-3p	CLIP-seq
MIRT1059047	hsa-miR-513a-5p	CLIP-seq
MIRT1059048	hsa-miR-561	CLIP-seq
MIRT1059049	hsa-miR-608	CLIP-seq
MIRT1059050	hsa-miR-615-5p	CLIP-seq
MIRT1059051	hsa-miR-620	CLIP-seq
MIRT1059052	hsa-miR-766	CLIP-seq
MIRT1059053	hsa-miR-769-5p	CLIP-seq
MIRT1059054	hsa-miR-889	CLIP-seq
MIRT1059055	hsa-miR-920	CLIP-seq
MIRT1059027	hsa-miR-3667-3p	CLIP-seq
MIRT1549714	hsa-miR-4632	CLIP-seq
MIRT1059008	hsa-miR-1180	CLIP-seq
MIRT2013769	hsa-miR-1181	CLIP-seq
MIRT2013770	hsa-miR-1207-3p	CLIP-seq
MIRT1059012	hsa-miR-128	CLIP-seq
MIRT2013771	hsa-miR-138	CLIP-seq
MIRT2013772	hsa-miR-149	CLIP-seq
MIRT1059014	hsa-miR-1910	CLIP-seq
MIRT2013773	hsa-miR-1915	CLIP-seq
MIRT1059015	hsa-miR-1972	CLIP-seq
MIRT1059018	hsa-miR-27a	CLIP-seq
MIRT1059019	hsa-miR-27b	CLIP-seq
MIRT1059020	hsa-miR-29a	CLIP-seq
MIRT1059021	hsa-miR-29b	CLIP-seq
MIRT1059022	hsa-miR-29c	CLIP-seq
MIRT2013774	hsa-miR-3064-5p	CLIP-seq
MIRT1059027	hsa-miR-3667-3p	CLIP-seq
MIRT1059030	hsa-miR-4259	CLIP-seq
MIRT1059032	hsa-miR-4300	CLIP-seq
MIRT2013775	hsa-miR-4435	CLIP-seq
MIRT2013776	hsa-miR-4456	CLIP-seq
MIRT2013777	hsa-miR-4514	CLIP-seq
MIRT1059035	hsa-miR-4532	CLIP-seq
MIRT2013778	hsa-miR-4645-5p	CLIP-seq
MIRT1059037	hsa-miR-4650-5p	CLIP-seq
MIRT2013779	hsa-miR-4673	CLIP-seq
MIRT2013780	hsa-miR-4692	CLIP-seq
MIRT1059043	hsa-miR-4717-5p	CLIP-seq
MIRT1059044	hsa-miR-4722-5p	CLIP-seq
MIRT2013781	hsa-miR-4726-3p	CLIP-seq
MIRT1059045	hsa-miR-4768-3p	CLIP-seq
MIRT2013782	hsa-miR-4794	CLIP-seq
MIRT1059047	hsa-miR-513a-5p	CLIP-seq
MIRT1059050	hsa-miR-615-5p	CLIP-seq
MIRT2013783	hsa-miR-760	CLIP-seq
MIRT1059052	hsa-miR-766	CLIP-seq
MIRT1059055	hsa-miR-920	CLIP-seq
MIRT1059023	hsa-miR-3127-5p	CLIP-seq
MIRT2245895	hsa-miR-3194-3p	CLIP-seq
MIRT1059029	hsa-miR-3918	CLIP-seq
MIRT2245896	hsa-miR-4267	CLIP-seq
MIRT1549714	hsa-miR-4632	CLIP-seq
MIRT2245897	hsa-miR-4642	CLIP-seq
MIRT1059008	hsa-miR-1180	CLIP-seq
MIRT2013769	hsa-miR-1181	CLIP-seq
MIRT2013770	hsa-miR-1207-3p	CLIP-seq
MIRT1059012	hsa-miR-128	CLIP-seq
MIRT2437689	hsa-miR-1299	CLIP-seq
MIRT2013771	hsa-miR-138	CLIP-seq
MIRT2013772	hsa-miR-149	CLIP-seq
MIRT1059014	hsa-miR-1910	CLIP-seq
MIRT2013773	hsa-miR-1915	CLIP-seq
MIRT1059015	hsa-miR-1972	CLIP-seq
MIRT1059018	hsa-miR-27a	CLIP-seq
MIRT1059019	hsa-miR-27b	CLIP-seq
MIRT1059020	hsa-miR-29a	CLIP-seq
MIRT1059021	hsa-miR-29b	CLIP-seq
MIRT1059022	hsa-miR-29c	CLIP-seq
MIRT2013774	hsa-miR-3064-5p	CLIP-seq
MIRT2437690	hsa-miR-3065-3p	CLIP-seq
MIRT2437691	hsa-miR-3193	CLIP-seq
MIRT2437692	hsa-miR-3689d	CLIP-seq
MIRT1059030	hsa-miR-4259	CLIP-seq
MIRT1059031	hsa-miR-4260	CLIP-seq
MIRT2013775	hsa-miR-4435	CLIP-seq
MIRT2013776	hsa-miR-4456	CLIP-seq
MIRT2013777	hsa-miR-4514	CLIP-seq
MIRT1059035	hsa-miR-4532	CLIP-seq
MIRT2013778	hsa-miR-4645-5p	CLIP-seq
MIRT1059037	hsa-miR-4650-5p	CLIP-seq
MIRT2013779	hsa-miR-4673	CLIP-seq
MIRT2013780	hsa-miR-4692	CLIP-seq
MIRT1059044	hsa-miR-4722-5p	CLIP-seq
MIRT2013781	hsa-miR-4726-3p	CLIP-seq
MIRT1059045	hsa-miR-4768-3p	CLIP-seq
MIRT2437693	hsa-miR-4782-5p	CLIP-seq
MIRT2013782	hsa-miR-4794	CLIP-seq
MIRT1059047	hsa-miR-513a-5p	CLIP-seq
MIRT2437694	hsa-miR-516b	CLIP-seq
MIRT1059050	hsa-miR-615-5p	CLIP-seq
MIRT2013783	hsa-miR-760	CLIP-seq
MIRT1059052	hsa-miR-766	CLIP-seq
MIRT1059011	hsa-miR-1275	CLIP-seq
MIRT1059026	hsa-miR-342-5p	CLIP-seq
MIRT1059038	hsa-miR-4651	CLIP-seq
MIRT1059039	hsa-miR-4664-5p	CLIP-seq
MIRT1059040	hsa-miR-4665-5p	CLIP-seq
MIRT1059049	hsa-miR-608	CLIP-seq
MIRT2013769	hsa-miR-1181	CLIP-seq
MIRT2013770	hsa-miR-1207-3p	CLIP-seq
MIRT1059009	hsa-miR-1228	CLIP-seq
MIRT1059011	hsa-miR-1275	CLIP-seq
MIRT1059012	hsa-miR-128	CLIP-seq
MIRT2013771	hsa-miR-138	CLIP-seq
MIRT2013772	hsa-miR-149	CLIP-seq
MIRT1059014	hsa-miR-1910	CLIP-seq
MIRT1059015	hsa-miR-1972	CLIP-seq
MIRT1059018	hsa-miR-27a	CLIP-seq
MIRT1059019	hsa-miR-27b	CLIP-seq
MIRT1059020	hsa-miR-29a	CLIP-seq
MIRT1059021	hsa-miR-29b	CLIP-seq
MIRT1059022	hsa-miR-29c	CLIP-seq
MIRT2013774	hsa-miR-3064-5p	CLIP-seq
MIRT1059026	hsa-miR-342-5p	CLIP-seq
MIRT1059032	hsa-miR-4300	CLIP-seq
MIRT2013775	hsa-miR-4435	CLIP-seq
MIRT2013776	hsa-miR-4456	CLIP-seq
MIRT1059037	hsa-miR-4650-5p	CLIP-seq
MIRT1059038	hsa-miR-4651	CLIP-seq
MIRT1059039	hsa-miR-4664-5p	CLIP-seq
MIRT1059040	hsa-miR-4665-5p	CLIP-seq
MIRT1059042	hsa-miR-4709-3p	CLIP-seq
MIRT1059043	hsa-miR-4717-5p	CLIP-seq
MIRT2013782	hsa-miR-4794	CLIP-seq
MIRT1059047	hsa-miR-513a-5p	CLIP-seq
MIRT1059049	hsa-miR-608	CLIP-seq
MIRT2013783	hsa-miR-760	CLIP-seq
MIRT1059052	hsa-miR-766	CLIP-seq
MIRT1059053	hsa-miR-769-5p	CLIP-seq
MIRT1059055	hsa-miR-920	CLIP-seq

Gene ontology (GO)

GO ID	Ontology	Definition	Evidence	Reference
GO:0000122	Process	Negative regulation of transcription by RNA polymerase II	IBA	21873635
GO:0000122	Process	Negative regulation of transcription by RNA polymerase II	TAS	25248477
GO:0001656	Process	Metanephros development	IEA
GO:0005515	Function	Protein binding	IPI	18255255, 20861012, 25416956, 25609649, 28514442, 31515488, 32296183, 32814053
GO:0005634	Component	Nucleus	IBA	21873635
GO:0005737	Component	Cytoplasm	IEA
GO:0006275	Process	Regulation of DNA replication	IEA
GO:0007275	Process	Multicellular organism development	TAS	1628620
GO:0007417	Process	Central nervous system development	IEA
GO:0007507	Process	Heart development	IEA
GO:0007517	Process	Muscle organ development	IEA
GO:0008134	Function	Transcription factor binding	IEA
GO:0009611	Process	Response to wounding	IEA
GO:0010628	Process	Positive regulation of gene expression	IEA
GO:0019904	Function	Protein domain specific binding	IEA
GO:0030154	Process	Cell differentiation	IBA	21873635
GO:0030182	Process	Neuron differentiation	IEA
GO:0030855	Process	Epithelial cell differentiation	IEA
GO:0030903	Process	Notochord development	IEA
GO:0032922	Process	Circadian regulation of gene expression	IBA	21873635
GO:0042476	Process	Odontogenesis	IEA
GO:0043065	Process	Positive regulation of apoptotic process	IEA
GO:0043433	Process	Negative regulation of DNA-binding transcription factor activity	IDA	14627819
GO:0045662	Process	Negative regulation of myoblast differentiation	IEA
GO:0045668	Process	Negative regulation of osteoblast differentiation	IEA
GO:0045892	Process	Negative regulation of transcription, DNA-templated	IDA	14627819
GO:0046983	Function	Protein dimerization activity	IEA
GO:0051726	Process	Regulation of cell cycle	IEA
GO:0072750	Process	Cellular response to leptomycin B	IEA
GO:0140110	Function	Transcription regulator activity	IBA	21873635
GO:0140416	Function	Transcription regulator inhibitor activity	TAS	25248477
GO:1901707	Function	Leptomycin B binding	IEA

Other IDs

• MIM: 600277
• HGNC: 5362
• e!Ensembl: ENSG00000117318

Protein

• UniProt ID: Q02535
• Protein name: DNA-binding protein inhibitor ID-3 (Class B basic helix-loop-helix protein 25) (bHLHb25) (Helix-loop-helix protein HEIR-1) (ID-like protein inhibitor HLH 1R21) (Inhibitor of DNA binding 3) (Inhibitor of differentiation 3)
• Protein function: Transcriptional regulator (lacking a basic DNA binding domain) which negatively regulates the basic helix-loop-helix (bHLH) transcription factors by forming heterodimers and inhibiting their DNA binding and transcriptional activity. Implicated i
• PDB: 2LFH
• Family and domains:

  Pfam

  Accession	ID	Position in sequence	Description	Type
  PF00010	HLH	40 → 81	Helix-loop-helix DNA-binding domain	Domain

• Sequence:

  MKALSPVRGCYEAVCCLSERSLAIARGRGKGPAAEEPLSLLDDMNHCYSRLRELVPGVPR
  GTQLSQVEILQRVIDYILDLQVVLAEPAPGPPDGPHLPIQTAELTPELVISNDKRSFCH

• Sequence length: 119

Pathways

KEGG:

TGF-beta signaling pathway
Signaling pathways regulating pluripotency of stem cells

Reactome:

NGF-stimulated transcription

Associated diseases

Causal
Disease name	Disease term	dbSNP ID	References
Adenocarcinoma	Adenocarcinoma, Adenocarcinoma, Basal Cell, Adenocarcinoma, Oxyphilic, Adenocarcinoma, Tubular	rs121913530, rs886039394, rs121913474	21552421
Burkitt`s lymphoma	Burkitt Lymphoma, Burkitt Leukemia	rs28933407, rs121918683, rs121918684	23143597, 23143595
Carcinoma	Carcinoma, Carcinoma, Cribriform, Carcinoma, Granular Cell, Carcinoma, Spindle-Cell, Undifferentiated carcinoma	rs121912654, rs555607708, rs786202962, rs1564055259	12376462, 21552421
Colonic neoplasms	Malignant tumor of colon, Colonic Neoplasms	rs267607789, rs774277300, rs781222233, rs1060502734, rs1060503333, rs1339238483	15059925
Lung cancer	Malignant neoplasm of lung	rs121913530, rs121913529, rs878855122, rs1057519784, rs770315135	21552421
Prostate cancer	Malignant neoplasm of prostate	rs121909139, rs121909140, rs121909141, rs121909142, rs121909143, rs606231169, rs606231170, rs137852584, rs137852578, rs137852580, rs137852581, rs137852582	17199135

Unknown
Disease name	Disease term	dbSNP ID	References
African burkitt`s lymphoma	African Burkitt`s lymphoma		23143595, 23143597
Anaplastic carcinoma	Anaplastic carcinoma		12376462
Lung neoplasms	Lung Neoplasms		21552421
Myocardial ischemia	Myocardial Ischemia		16214533
Nervous system disorder	nervous system disorder		21245421
Prostatic neoplasms	Prostatic Neoplasms		17199135
Thyroid diseases	Thyroid Diseases		23397585