ANPEP (alanyl aminopeptidase, membrane)

Gene

• Entrez ID: 290
• Gene name: Alanyl aminopeptidase, membrane
• Gene symbol: ANPEP
• Synonyms: AP-M, AP-N, APN, CD13, GP150, LAP1, P150, PEPN, hAPN
• Chromosome: 15
• Chromosome location: 15q26.1
• Summary: Aminopeptidase N is located in the small-intestinal and renal microvillar membrane, and also in other plasma membranes. In the small intestine aminopeptidase N plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastri

miRNA

miRTarBase ID	miRNA	Experiments	Reference
MIRT005211	hsa-miR-30a-5p	pSILAC	18668040
MIRT001389	hsa-miR-1-3p	pSILAC	18668040
MIRT020955	hsa-miR-155-5p	Proteomics	18668040
MIRT022990	hsa-miR-124-3p	Proteomics;Microarray	18668037
MIRT001389	hsa-miR-1-3p	Proteomics;Other	18668040
MIRT005211	hsa-miR-30a-5p	Proteomics;Other	18668040
MIRT030575	hsa-miR-24-3p	Microarray	19748357
MIRT031867	hsa-miR-16-5p	Proteomics	18668040
MIRT786199	hsa-miR-1587	CLIP-seq
MIRT786200	hsa-miR-299-3p	CLIP-seq
MIRT786201	hsa-miR-3192	CLIP-seq
MIRT786202	hsa-miR-3652	CLIP-seq
MIRT786203	hsa-miR-3689a-3p	CLIP-seq
MIRT786204	hsa-miR-3689c	CLIP-seq
MIRT786205	hsa-miR-3689d	CLIP-seq
MIRT786206	hsa-miR-3907	CLIP-seq
MIRT786207	hsa-miR-3925-5p	CLIP-seq
MIRT786208	hsa-miR-4269	CLIP-seq
MIRT786209	hsa-miR-4417	CLIP-seq
MIRT786210	hsa-miR-4430	CLIP-seq
MIRT786211	hsa-miR-4492	CLIP-seq
MIRT786212	hsa-miR-4498	CLIP-seq
MIRT786213	hsa-miR-4514	CLIP-seq
MIRT786214	hsa-miR-4643	CLIP-seq
MIRT786215	hsa-miR-4656	CLIP-seq
MIRT786216	hsa-miR-4675	CLIP-seq
MIRT786217	hsa-miR-4692	CLIP-seq
MIRT786218	hsa-miR-4728-5p	CLIP-seq
MIRT786219	hsa-miR-4741	CLIP-seq
MIRT786220	hsa-miR-4742-5p	CLIP-seq
MIRT786221	hsa-miR-513a-5p	CLIP-seq
MIRT786222	hsa-miR-642b	CLIP-seq
MIRT786223	hsa-miR-661	CLIP-seq
MIRT786224	hsa-miR-762	CLIP-seq
MIRT2435353	hsa-miR-125a-5p	CLIP-seq
MIRT2435354	hsa-miR-125b	CLIP-seq
MIRT2435355	hsa-miR-1273	CLIP-seq
MIRT2435356	hsa-miR-1291	CLIP-seq
MIRT786199	hsa-miR-1587	CLIP-seq
MIRT2435357	hsa-miR-1908	CLIP-seq
MIRT2435358	hsa-miR-1915	CLIP-seq
MIRT2435359	hsa-miR-2355-3p	CLIP-seq
MIRT2435360	hsa-miR-24	CLIP-seq
MIRT2435361	hsa-miR-3126-3p	CLIP-seq
MIRT2435362	hsa-miR-3135	CLIP-seq
MIRT2435363	hsa-miR-3135b	CLIP-seq
MIRT2435364	hsa-miR-3141	CLIP-seq
MIRT2435365	hsa-miR-3176	CLIP-seq
MIRT2435366	hsa-miR-3184	CLIP-seq
MIRT2435367	hsa-miR-328	CLIP-seq
MIRT2435368	hsa-miR-3622b-5p	CLIP-seq
MIRT786202	hsa-miR-3652	CLIP-seq
MIRT2435369	hsa-miR-378g	CLIP-seq
MIRT2435370	hsa-miR-3922-3p	CLIP-seq
MIRT786207	hsa-miR-3925-5p	CLIP-seq
MIRT2435371	hsa-miR-423-5p	CLIP-seq
MIRT2435372	hsa-miR-4254	CLIP-seq
MIRT2435373	hsa-miR-4284	CLIP-seq
MIRT2435374	hsa-miR-4308	CLIP-seq
MIRT2435375	hsa-miR-4319	CLIP-seq
MIRT2435376	hsa-miR-4323	CLIP-seq
MIRT2435377	hsa-miR-4324	CLIP-seq
MIRT786209	hsa-miR-4417	CLIP-seq
MIRT786210	hsa-miR-4430	CLIP-seq
MIRT2435378	hsa-miR-4486	CLIP-seq
MIRT2435379	hsa-miR-4487	CLIP-seq
MIRT786211	hsa-miR-4492	CLIP-seq
MIRT786212	hsa-miR-4498	CLIP-seq
MIRT786213	hsa-miR-4514	CLIP-seq
MIRT2435380	hsa-miR-4633-3p	CLIP-seq
MIRT2435381	hsa-miR-4642	CLIP-seq
MIRT2435382	hsa-miR-4646-3p	CLIP-seq
MIRT2435383	hsa-miR-4649-5p	CLIP-seq
MIRT2435384	hsa-miR-4650-5p	CLIP-seq
MIRT786215	hsa-miR-4656	CLIP-seq
MIRT2435385	hsa-miR-4659a-5p	CLIP-seq
MIRT2435386	hsa-miR-4659b-5p	CLIP-seq
MIRT786216	hsa-miR-4675	CLIP-seq
MIRT2435387	hsa-miR-4685-5p	CLIP-seq
MIRT2435388	hsa-miR-4690-3p	CLIP-seq
MIRT786217	hsa-miR-4692	CLIP-seq
MIRT2435389	hsa-miR-4732-3p	CLIP-seq
MIRT786219	hsa-miR-4741	CLIP-seq
MIRT2435390	hsa-miR-4755-3p	CLIP-seq
MIRT786221	hsa-miR-513a-5p	CLIP-seq
MIRT2435391	hsa-miR-552	CLIP-seq
MIRT2435392	hsa-miR-663	CLIP-seq
MIRT2435393	hsa-miR-670	CLIP-seq
MIRT2435394	hsa-miR-676	CLIP-seq
MIRT786224	hsa-miR-762	CLIP-seq
MIRT2435395	hsa-miR-764	CLIP-seq
MIRT2435396	hsa-miR-767-3p	CLIP-seq
MIRT2435360	hsa-miR-24	CLIP-seq
MIRT786207	hsa-miR-3925-5p	CLIP-seq
MIRT2435373	hsa-miR-4284	CLIP-seq
MIRT786213	hsa-miR-4514	CLIP-seq
MIRT786217	hsa-miR-4692	CLIP-seq
MIRT786221	hsa-miR-513a-5p	CLIP-seq
MIRT786199	hsa-miR-1587	CLIP-seq
MIRT2435359	hsa-miR-2355-3p	CLIP-seq
MIRT2435360	hsa-miR-24	CLIP-seq
MIRT2435363	hsa-miR-3135b	CLIP-seq
MIRT2435366	hsa-miR-3184	CLIP-seq
MIRT786202	hsa-miR-3652	CLIP-seq
MIRT786207	hsa-miR-3925-5p	CLIP-seq
MIRT2435371	hsa-miR-423-5p	CLIP-seq
MIRT2435373	hsa-miR-4284	CLIP-seq
MIRT786209	hsa-miR-4417	CLIP-seq
MIRT786210	hsa-miR-4430	CLIP-seq
MIRT786211	hsa-miR-4492	CLIP-seq
MIRT786212	hsa-miR-4498	CLIP-seq
MIRT786213	hsa-miR-4514	CLIP-seq
MIRT2435382	hsa-miR-4646-3p	CLIP-seq
MIRT786215	hsa-miR-4656	CLIP-seq
MIRT786216	hsa-miR-4675	CLIP-seq
MIRT786217	hsa-miR-4692	CLIP-seq
MIRT786219	hsa-miR-4741	CLIP-seq
MIRT786221	hsa-miR-513a-5p	CLIP-seq
MIRT2435394	hsa-miR-676	CLIP-seq
MIRT786224	hsa-miR-762	CLIP-seq
MIRT2675059	hsa-miR-1197	CLIP-seq
MIRT786199	hsa-miR-1587	CLIP-seq
MIRT2435359	hsa-miR-2355-3p	CLIP-seq
MIRT2435360	hsa-miR-24	CLIP-seq
MIRT2435363	hsa-miR-3135b	CLIP-seq
MIRT2435366	hsa-miR-3184	CLIP-seq
MIRT786202	hsa-miR-3652	CLIP-seq
MIRT786207	hsa-miR-3925-5p	CLIP-seq
MIRT2435371	hsa-miR-423-5p	CLIP-seq
MIRT2435373	hsa-miR-4284	CLIP-seq
MIRT786209	hsa-miR-4417	CLIP-seq
MIRT786210	hsa-miR-4430	CLIP-seq
MIRT786211	hsa-miR-4492	CLIP-seq
MIRT786212	hsa-miR-4498	CLIP-seq
MIRT786213	hsa-miR-4514	CLIP-seq
MIRT2435382	hsa-miR-4646-3p	CLIP-seq
MIRT786215	hsa-miR-4656	CLIP-seq
MIRT786216	hsa-miR-4675	CLIP-seq
MIRT786217	hsa-miR-4692	CLIP-seq
MIRT786219	hsa-miR-4741	CLIP-seq
MIRT786221	hsa-miR-513a-5p	CLIP-seq
MIRT2435394	hsa-miR-676	CLIP-seq
MIRT786224	hsa-miR-762	CLIP-seq

Transcription factors

Transcription factor	Regulation	Reference
ETS1	Activation	14507917
ETS2	Activation	14507917
MYB	Unknown	10477683

Gene ontology (GO)

GO ID	Ontology	Definition	Evidence	Reference
GO:0001525	Process	Angiogenesis	IEA
GO:0001618	Function	Virus receptor activity	IEA
GO:0004177	Function	Aminopeptidase activity	TAS	10734069
GO:0005615	Component	Extracellular space	HDA	16502470
GO:0005615	Component	Extracellular space	IDA	25645918
GO:0005737	Component	Cytoplasm	IBA	21873635
GO:0005765	Component	Lysosomal membrane	HDA	17897319
GO:0005793	Component	Endoplasmic reticulum-Golgi intermediate compartment	IDA	15308636
GO:0005886	Component	Plasma membrane	IBA	21873635
GO:0005886	Component	Plasma membrane	IDA	2564851
GO:0005886	Component	Plasma membrane	TAS
GO:0006508	Process	Proteolysis	IBA	21873635
GO:0007165	Process	Signal transduction	IBA	21873635
GO:0008217	Process	Regulation of blood pressure	IBA	21873635
GO:0008237	Function	Metallopeptidase activity	TAS	2564851
GO:0008270	Function	Zinc ion binding	IBA	21873635
GO:0016021	Component	Integral component of membrane	IEA
GO:0030154	Process	Cell differentiation	IEA
GO:0030667	Component	Secretory granule membrane	TAS
GO:0038023	Function	Signaling receptor activity	TAS	1350662
GO:0042277	Function	Peptide binding	IBA	21873635
GO:0043171	Process	Peptide catabolic process	IBA	21873635
GO:0043312	Process	Neutrophil degranulation	TAS
GO:0046718	Process	Viral entry into host cell	IEA
GO:0070006	Function	Metalloaminopeptidase activity	IBA	21873635
GO:0070062	Component	Extracellular exosome	HDA	19056867, 19199708, 23533145
GO:0070062	Component	Extracellular exosome	IDA	15326289, 21082674

Other IDs

• MIM: 151530
• HGNC: 500
• e!Ensembl: ENSG00000166825

Protein

• UniProt ID: P15144
• Protein name: Aminopeptidase N (AP-N) (hAPN) (EC 3.4.11.2) (Alanyl aminopeptidase) (Aminopeptidase M) (AP-M) (Microsomal aminopeptidase) (Myeloid plasma membrane glycoprotein CD13) (gp150) (CD antigen CD13)
• Protein function: Broad specificity aminopeptidase which plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. Also involved in the processing of various peptides including peptide hormones, suc
• PDB: 4FYQ, 4FYR, 4FYS, 4FYT, 5LHD, 6ATK, 6U7E, 6U7F, 6U7G, 6XWD, 7AEW, 7VPQ, 8WDE
• Family and domains:

  Pfam

  Accession	ID	Position in sequence	Description	Type
  PF17900	Peptidase_M1_N	81 → 279		Domain
  PF01433	Peptidase_M1	316 → 543	Peptidase family M1 domain	Domain
  PF11838	ERAP1_C	619 → 946	ERAP1-like C-terminal domain	Domain

• Sequence:

  MAKGFYISKSLGILGILLGVAAVCTIIALSVVYSQEKNKNANSSPVASTTPSASATTNPA
  SATTLDQSKAWNRYRLPNTLKPDSYRVTLRPYLTPNDRGLYVFKGSSTVRFTCKEATDVI
  IIHSKKLNYTLSQGHRVVLRGVGGSQPPDIDKTELVEPTEYLVVHLKGSLVKDSQYEMDS
  EFEGELADDLAGFYRSEYMEGNVRKVVATTQMQAADARKSFPCFDEPAMKAEFNITLIHP
  KDLTALSNMLPKGPSTPLPEDPNWNVTEFHTTPKMSTYLLAFIVSEFDYVEKQASNGVLI
  RIWARPSAIAAGHGDYALNVTGPILNFFAGHYDTPYPLPKSDQIGLPDFNAGAMENWGLV
  TYRENSLLFDPLSSSSSNKERVVTVIAHELAHQWFGNLVTIEWWNDLWLNEGFASYVEYL
  GADYAEPTWNLKDLMVLNDVYRVMAVDALASSHPLSTPASEINTPAQISELFDAISYSKG
  ASVLRMLSSFLSEDVFKQGLASYLHTFAYQNTIYLNLWDHLQEAVNNRSIQLPTTVRDIM
  NRWTLQMGFPVITVDTSTGTLSQEHFLLDPDSNVTRPSEFNYVWIVPITSIRDGRQQQDY
  WLIDVRAQNDLFSTSGNEWVLLNLNVTGYYRVNYDEENWRKIQTQLQRDHSAIPVINRAQ
  IINDAFNLASAHKVPVTLALNNTLFLIEERQYMPWEAALSSLSYFKLMFDRSEVYGPMKN
  YLKKQVTPLFIHFRNNTNNWREIPENLMDQYSEVNAISTACSNGVPECEEMVSGLFKQWM
  ENPNNNPIHPNLRSTVYCNAIAQGGEEEWDFAWEQFRNATLVNEADKLRAALACSKELWI
  LNRYLSYTLNPDLIRKQDATSTIISITNNVIGQGLVWDFVQSNWKKLFNDYGGGSFSFSN
  LIQAVTRRFSTEYELQQLEQFKKDNEETGFGSGTRALEQALEKTKANIKWVKENKEVVLQ
  WFTENSK

• Sequence length: 967

Pathways

KEGG:

Glutathione metabolism
Metabolic pathways
Renin-angiotensin system
Hematopoietic cell lineage

Reactome:

Neutrophil degranulation

Associated diseases

Unknown
Disease name	Disease term	dbSNP ID	References
Urinary bladder cancer	Malignant neoplasm of urinary bladder		26039340
Bladder neoplasm	Bladder Neoplasm		26039340
Mental depression	Unipolar Depression, Major Depressive Disorder	rs587778876, rs587778877	23794217