TPX2 (TPX2 microtubule nucleation factor)

Gene

• Entrez ID: 22974
• Gene name: TPX2 microtubule nucleation factor
• Gene symbol: TPX2
• Synonyms: C20orf1, C20orf2, DIL-2, DIL2, FLS353, GD:C20orf1, HCA519, HCTP4, REPP86, p100
• Chromosome: 20
• Chromosome location: 20q11.21

miRNA

miRTarBase ID	miRNA	Experiments	Reference
MIRT041448	hsa-miR-193b-3p	CLASH	23622248
MIRT733798	hsa-miR-338-3p	Microarray, qRT-PCR	33789319
MIRT736547	hsa-miR-548a-3p	Luciferase reporter assay, Western blotting, qRT-PCR	32857606
MIRT737338	hsa-miR-216b-3p	Luciferase reporter assay, Western blotting, Immunohistochemistry (IHC), qRT-PCR, Flow cytometry	32169806
MIRT755887	hsa-miR-29c-3p	Luciferase reporter assay, Western blotting, qRT-PCR	35340555
MIRT1450484	hsa-miR-1266	CLIP-seq
MIRT1450485	hsa-miR-1289	CLIP-seq
MIRT1450486	hsa-miR-1304	CLIP-seq
MIRT1450487	hsa-miR-1323	CLIP-seq
MIRT1450488	hsa-miR-216a	CLIP-seq
MIRT1450489	hsa-miR-216b	CLIP-seq
MIRT1450490	hsa-miR-219-5p	CLIP-seq
MIRT1450491	hsa-miR-2278	CLIP-seq
MIRT1450492	hsa-miR-2467-3p	CLIP-seq
MIRT1450493	hsa-miR-3065-5p	CLIP-seq
MIRT1450494	hsa-miR-3150a-3p	CLIP-seq
MIRT1450495	hsa-miR-3159	CLIP-seq
MIRT1450496	hsa-miR-3160-5p	CLIP-seq
MIRT1450497	hsa-miR-3175	CLIP-seq
MIRT1450498	hsa-miR-3198	CLIP-seq
MIRT1450499	hsa-miR-3622a-5p	CLIP-seq
MIRT1450500	hsa-miR-3664-3p	CLIP-seq
MIRT1450501	hsa-miR-3667-5p	CLIP-seq
MIRT1450502	hsa-miR-3675-3p	CLIP-seq
MIRT1450503	hsa-miR-3678-3p	CLIP-seq
MIRT1450504	hsa-miR-3713	CLIP-seq
MIRT1450505	hsa-miR-3920	CLIP-seq
MIRT1450506	hsa-miR-4269	CLIP-seq
MIRT1450507	hsa-miR-4282	CLIP-seq
MIRT1450508	hsa-miR-4294	CLIP-seq
MIRT1450509	hsa-miR-4309	CLIP-seq
MIRT1450510	hsa-miR-4317	CLIP-seq
MIRT1450511	hsa-miR-4326	CLIP-seq
MIRT1450512	hsa-miR-4445	CLIP-seq
MIRT1450513	hsa-miR-4464	CLIP-seq
MIRT1450514	hsa-miR-4514	CLIP-seq
MIRT1450515	hsa-miR-4518	CLIP-seq
MIRT1450516	hsa-miR-4660	CLIP-seq
MIRT1450517	hsa-miR-4692	CLIP-seq
MIRT1450518	hsa-miR-4693-3p	CLIP-seq
MIRT1450519	hsa-miR-4699-3p	CLIP-seq
MIRT1450520	hsa-miR-4729	CLIP-seq
MIRT1450521	hsa-miR-4742-5p	CLIP-seq
MIRT1450522	hsa-miR-4748	CLIP-seq
MIRT1450523	hsa-miR-4782-3p	CLIP-seq
MIRT1450524	hsa-miR-4796-3p	CLIP-seq
MIRT1450525	hsa-miR-491-5p	CLIP-seq
MIRT1450526	hsa-miR-508-3p	CLIP-seq
MIRT1450527	hsa-miR-513c	CLIP-seq
MIRT1450528	hsa-miR-514b-5p	CLIP-seq
MIRT1450529	hsa-miR-548e	CLIP-seq
MIRT1450530	hsa-miR-548f	CLIP-seq
MIRT1450531	hsa-miR-548o	CLIP-seq
MIRT1450532	hsa-miR-554	CLIP-seq
MIRT1450533	hsa-miR-564	CLIP-seq
MIRT1450534	hsa-miR-570	CLIP-seq
MIRT1450535	hsa-miR-642b	CLIP-seq
MIRT1450536	hsa-miR-651	CLIP-seq
MIRT1450537	hsa-miR-759	CLIP-seq
MIRT1450538	hsa-miR-939	CLIP-seq
MIRT2134659	hsa-miR-1253	CLIP-seq
MIRT1450485	hsa-miR-1289	CLIP-seq
MIRT1450486	hsa-miR-1304	CLIP-seq
MIRT2134660	hsa-miR-181a	CLIP-seq
MIRT2134661	hsa-miR-181b	CLIP-seq
MIRT2134662	hsa-miR-181c	CLIP-seq
MIRT2134663	hsa-miR-181d	CLIP-seq
MIRT1450491	hsa-miR-2278	CLIP-seq
MIRT1450492	hsa-miR-2467-3p	CLIP-seq
MIRT2134664	hsa-miR-299-5p	CLIP-seq
MIRT2134665	hsa-miR-3163	CLIP-seq
MIRT1450498	hsa-miR-3198	CLIP-seq
MIRT2134666	hsa-miR-3614-3p	CLIP-seq
MIRT1450501	hsa-miR-3667-5p	CLIP-seq
MIRT1450503	hsa-miR-3678-3p	CLIP-seq
MIRT1450504	hsa-miR-3713	CLIP-seq
MIRT2134667	hsa-miR-3919	CLIP-seq
MIRT2134668	hsa-miR-4262	CLIP-seq
MIRT1450508	hsa-miR-4294	CLIP-seq
MIRT1450509	hsa-miR-4309	CLIP-seq
MIRT1450510	hsa-miR-4317	CLIP-seq
MIRT2134669	hsa-miR-433	CLIP-seq
MIRT2134670	hsa-miR-4424	CLIP-seq
MIRT1450513	hsa-miR-4464	CLIP-seq
MIRT2134671	hsa-miR-4528	CLIP-seq
MIRT1450522	hsa-miR-4748	CLIP-seq
MIRT2134672	hsa-miR-4802-3p	CLIP-seq
MIRT1450532	hsa-miR-554	CLIP-seq
MIRT2134673	hsa-miR-606	CLIP-seq
MIRT1450535	hsa-miR-642b	CLIP-seq
MIRT1450537	hsa-miR-759	CLIP-seq
MIRT2134660	hsa-miR-181a	CLIP-seq
MIRT2134661	hsa-miR-181b	CLIP-seq
MIRT2134662	hsa-miR-181c	CLIP-seq
MIRT2134663	hsa-miR-181d	CLIP-seq
MIRT2355963	hsa-miR-1972	CLIP-seq
MIRT2134664	hsa-miR-299-5p	CLIP-seq
MIRT2134665	hsa-miR-3163	CLIP-seq
MIRT1450501	hsa-miR-3667-5p	CLIP-seq
MIRT1450504	hsa-miR-3713	CLIP-seq
MIRT2355964	hsa-miR-3908	CLIP-seq
MIRT2355965	hsa-miR-3936	CLIP-seq
MIRT2355966	hsa-miR-3942-5p	CLIP-seq
MIRT2134668	hsa-miR-4262	CLIP-seq
MIRT1450508	hsa-miR-4294	CLIP-seq
MIRT1450510	hsa-miR-4317	CLIP-seq
MIRT2355967	hsa-miR-4645-5p	CLIP-seq
MIRT1450516	hsa-miR-4660	CLIP-seq
MIRT2355968	hsa-miR-4673	CLIP-seq
MIRT2355969	hsa-miR-4688	CLIP-seq
MIRT1450518	hsa-miR-4693-3p	CLIP-seq
MIRT2355970	hsa-miR-4703-5p	CLIP-seq
MIRT2355971	hsa-miR-4766-3p	CLIP-seq
MIRT2355972	hsa-miR-548t	CLIP-seq
MIRT1450532	hsa-miR-554	CLIP-seq
MIRT1450535	hsa-miR-642b	CLIP-seq
MIRT1450537	hsa-miR-759	CLIP-seq
MIRT2134664	hsa-miR-299-5p	CLIP-seq
MIRT2134665	hsa-miR-3163	CLIP-seq
MIRT1450501	hsa-miR-3667-5p	CLIP-seq
MIRT1450532	hsa-miR-554	CLIP-seq
MIRT1450535	hsa-miR-642b	CLIP-seq
MIRT1450537	hsa-miR-759	CLIP-seq

Gene ontology (GO)

GO ID	Ontology	Definition	Evidence	Reference
GO:0000278	Process	Mitotic cell cycle	TAS	9207457
GO:0000922	Component	Spindle pole	IDA	14718566
GO:0005515	Function	Protein binding	IPI	14580337, 17474147, 19357306, 20360068, 21242313, 22110403, 25416956, 25986610, 27837025, 28514442, 32296183, 32814053
GO:0005634	Component	Nucleus	TAS	9207457
GO:0005654	Component	Nucleoplasm	IDA
GO:0005654	Component	Nucleoplasm	TAS
GO:0005819	Component	Spindle	ISS	18663142
GO:0005829	Component	Cytosol	TAS
GO:0005880	Component	Nuclear microtubule	IBA	21873635
GO:0006915	Process	Apoptotic process	IEA
GO:0007020	Process	Microtubule nucleation	IDA	26414402
GO:0007026	Process	Negative regulation of microtubule depolymerization	IDA	26414402
GO:0008017	Function	Microtubule binding	IBA	21873635
GO:0010389	Process	Regulation of G2/M transition of mitotic cell cycle	TAS
GO:0015630	Component	Microtubule cytoskeleton	IDA
GO:0019901	Function	Protein kinase binding	IPI	14580337, 18662907, 19801554
GO:0030295	Function	Protein kinase activator activity	IBA	21873635
GO:0032147	Process	Activation of protein kinase activity	IDA	14580337, 19801554
GO:0043203	Component	Axon hillock	IEA
GO:0045171	Component	Intercellular bridge	IDA
GO:0051301	Process	Cell division	IEA
GO:0060236	Process	Regulation of mitotic spindle organization	ISS	18663142
GO:0061676	Function	Importin-alpha family protein binding	IDA	26165940
GO:0072686	Component	Mitotic spindle	IBA	21873635
GO:0072686	Component	Mitotic spindle	IDA
GO:0090307	Process	Mitotic spindle assembly	IBA	21873635
GO:0090307	Process	Mitotic spindle assembly	IDA	26165940
GO:1901796	Process	Regulation of signal transduction by p53 class mediator	TAS

Other IDs

• MIM: 605917
• HGNC: 1249
• e!Ensembl: ENSG00000088325

Protein

• UniProt ID: Q9ULW0
• Protein name: Targeting protein for Xklp2 (Differentially expressed in cancerous and non-cancerous lung cells 2) (DIL-2) (Hepatocellular carcinoma-associated antigen 519) (Hepatocellular carcinoma-associated antigen 90) (Protein fls353) (Restricted expression prolifera
• Protein function: Spindle assembly factor required for normal assembly of mitotic spindles. Required for normal assembly of microtubules during apoptosis. Required for chromatin and/or kinetochore dependent microtubule nucleation. Mediates AURKA localization to s
• PDB: 1OL5, 3E5A, 3HA6, 4C3P, 5LXM, 6BJC, 6VPG, 6VPH, 6VPI, 6VPJ, 6VPL, 6VPM, 6XKA
• Family and domains:

  Pfam

  Accession	ID	Position in sequence	Description	Type
  PF09041	Aurora-A_bind	1 → 68	Aurora-A binding	Disordered
  PF12214	TPX2_importin	361 → 489	Cell cycle regulated microtubule associated protein	Family
  PF06886	TPX2	540 → 592	Targeting protein for Xklp2 (TPX2) domain	Domain
  PF06886	TPX2	660 → 740	Targeting protein for Xklp2 (TPX2) domain	Domain

• Sequence:

  MSQVKSSYSYDAPSDFINFSSLDDEGDTQNIDSWFEEKANLENKLLGKNGTGGLFQGKTP
  LRKANLQQAIVTPLKPVDNTYYKEAEKENLVEQSIPSNACSSLEVEAAISRKTPAQPQRR
  SLRLSAQKDLEQKEKHHVKMKAKRCATPVIIDEILPSKKMKVSNNKKKPEEEGSAHQDTA
  EKNASSPEKAKGRHTVPCMPPAKQKFLKSTEEQELEKSMKMQQEVVEMRKKNEEFKKLAL
  AGIGQPVKKSVSQVTKSVDFHFRTDERIKQHPKNQEEYKEVNFTSELRKHPSSPARVTKG
  CTIVKPFNLSQGKKRTFDETVSTYVPLAQQVEDFHKRTPNRYHLRSKKDDINLLPSKSSV
  TKICRDPQTPVLQTKHRARAVTCKSTAELEAEELEKLQQYKFKARELDPRILEGGPILPK
  KPPVKPPTEPIGFDLEIEKRIQERESKKKTEDEHFEFHSRPCPTKILEDVVGVPEKKVLP
  ITVPKSPAFALKNRIRMPTKEDEEEDEPVVIKAQPVPHYGVPFKPQIPEARTVEICPFSF
  DSRDKERQLQKEKKIKELQKGEVPKFKALPLPHFDTINLPEKKVKNVTQIEPFCLETDRR
  GALKAQTWKHQLEEELRQQKEAACFKARPNTVISQEPFVPKKEKKSVAEGLSGSLVQEPF
  QLATEKRAKERQELEKRMAEVEAQKAQQLEEARLQEEEQKKEELARLRRELVHKANPIRK
  YQGLEIKSSDQPLTVPVSPKFSTRFHC

• Sequence length: 747

Pathways

Reactome:

Regulation of TP53 Activity through Phosphorylation
AURKA Activation by TPX2

Associated diseases

Causal
Disease name	Disease term	dbSNP ID	References
Colorectal cancer	Colorectal Carcinoma	rs137854568, rs137854573, rs137854575, rs387906234, rs1801155, rs121908380, rs121908702, rs267606674, rs4939827, rs794729661, rs121909055, rs281865417, rs267606884, rs28934575, rs587776769, rs104893815, rs587776800, rs63750198, rs63751109, rs863223312, rs63750710, rs63751615, rs63750206, rs63750781, rs63750899, rs63750691, rs63750217, rs121912965, rs63749939, rs63751194, rs63750693, rs63750540, rs63751221, rs193922370, rs80359596, rs397514632, rs483352909, rs200495564, rs397514684, rs397516436, rs398122386, rs79512956, rs74953290, rs587779001, rs63750677, rs63749837, rs267607816, rs63751715, rs267607819, rs267607815, rs267607822, rs63749906, rs587778883, rs63750472, rs63751012, rs63750715, rs63750580, rs267607706, rs267607709, rs267607710, rs587778894, rs63750749, rs63750483, rs63751015, rs63751153, rs63751094, rs63751118, rs63750316, rs63749981, rs587778906, rs267607821, rs587778908, rs63750020, rs587778909, rs63750713, rs267607825, rs63751592, rs281864936, rs587778913, rs587778914, rs63749795, rs63750855, rs63749916, rs63749923, rs63751472, rs63751689, rs267607832, rs267607837, rs267607836, rs587778923, rs63750028, rs587778928, rs587778929, rs587778930, rs63751277, rs587778933, rs267607842, rs267607843, rs63750192, rs587778934, rs63750193, rs587778937, rs587778938, rs267607845, rs63751244, rs63751393, rs63751460, rs267607849, rs267607853, rs267607856, rs267607850, rs63751657, rs267607854, rs267607852, rs587778942, rs63750309, rs63750587, rs63749863, rs63751486, rs63750016, rs63749868, rs63750375, rs63750035, rs63750604, rs63750386, rs63750150, rs63750486, rs63751428, rs267607866, rs63749986, rs63751594, rs63750152, rs63750850, rs267607867, rs267607868, rs63751632, rs267607871, rs63751892, rs587778956, rs63750469, rs587778958, rs63749792, rs267607875, rs63751255, rs281864938, rs63751202, rs63750726, rs63751310, rs63749900, rs587778964, rs267607879, rs267607878, rs587778966, rs267607883, rs267607887, rs63750061, rs63750663, rs587778968, rs587778971, rs63750809, rs63749867, rs63750864, rs587778972, rs63751275, rs267607718, rs267607722, rs63750769, rs267607717, rs587778973, rs267607716, rs267607720, rs63749995, rs63750859, rs587778975, rs63750114, rs587778976, rs63750603, rs267607889, rs267607723, rs63750561, rs63750499, rs63751642, rs63751022, rs587778981, rs63750971, rs267607898, rs267607906, rs267607903, rs587778989, rs587778992, rs267607894, rs267607901, rs267607892, rs63750437, rs587778997, rs587778998, rs63750005, rs63750641, rs63751421, rs11541859, rs63750266, rs111052004, rs267607726, rs267607727, rs63750453, rs267607734, rs267607735, rs63751665, rs267607736, rs267607732, rs63749816, rs63750539, rs267607739, rs587779006, rs587779008, rs267607745, rs267607742, rs63751595, rs267607743, rs587779010, rs587779012, rs63750057, rs63749818, rs63751124, rs587779014, rs587779015, rs63749820, rs63751302, rs267607750, rs267607751, rs267607749, rs63750891, rs63749959, rs63749804, rs267607765, rs267607760, rs587779021, rs267607759, rs63750515, rs587779023, rs63751021, rs63751480, rs267607772, rs267607773, rs587779024, rs63751653, rs587779027, rs267607767, rs587779029, rs63750706, rs63750385, rs267607774, rs267607778, rs267607780, rs587779034, rs63751711, rs267607784, rs587779035, rs63750823, rs63750822, rs267607787, rs63750303, rs63749839, rs63749827, rs267607789, rs267607790, rs267607791, rs267607786, rs267607771, rs267607795, rs267607794, rs267607788, rs267607799, rs267607801, rs587779045, rs63750034, rs587779047, rs63750216, rs63751707, rs63751598, rs267607803, rs267607777, rs63750144, rs267607805, rs63750547, rs63750489, rs63750993, rs587779054, rs63751259, rs63749926, rs63750796, rs587779058, rs63750745, rs63750582, rs180177084, rs587779866, rs200389141, rs587779950, rs587780104, rs587780183, rs587778536, rs587780683, rs587781554, rs267607712, rs587777627, rs587783057, rs730881734, rs41542214, rs730881273, rs786203456, rs786201990, rs786202767, rs748005072, rs786204317, rs786204318, rs797045117, rs63750549, rs863225383, rs863225384, rs863225373, rs863225376, rs863225377, rs863225378, rs863225379, rs863225380, rs863225381, rs63750059, rs267607823, rs864622457, rs869312767, rs869312753, rs876661059, rs876658915, rs876658923, rs876660860, rs876660822, rs876660458, rs876660214, rs876658657, rs876658247, rs876659226, rs876658821, rs876660589, rs876659068, rs876659681, rs876659608, rs878853794, rs878853778, rs878853780, rs878853785, rs886039423, rs886039424, rs1057517543, rs1057517541, rs756843954, rs1057517617, rs1057517558, rs1057519256, rs1060500689, rs764085979, rs1060500707, rs1060500699, rs1060500706, rs1060500703, rs1064795341, rs1064793607, rs1064794348, rs1064795441, rs1064794373, rs1064793172, rs1064794122, rs1064795515, rs1064794331, rs63750978, rs1114167435, rs1553641362, rs63751448, rs1553648029, rs1553648058, rs587778903, rs1553653237, rs1553664353, rs267607744, rs1553647995, rs1553648047, rs1553653084, rs1553663750, rs1553664436, rs1553488015, rs1553637293, rs63750310, rs63750443, rs63751596, rs1553646681, rs550890395, rs1064796057, rs1553642079, rs1553648023, rs587782087, rs746536721, rs1553653037, rs1248251121, rs1553646602, rs1434898623, rs1553665683, rs1553648068, rs1553645331, rs1553644123, rs1553658246, rs1553651299, rs1553648149, rs1553663159, rs1302248679, rs1553664119, rs1553658009, rs1553665977, rs1416171624, rs1553663834, rs1553664617, rs1553664702, rs1553647969, rs1553648040, rs1437454428, rs1553641273, rs63751101, rs1553646764, rs1553648225, rs1554082118, rs1553648201, rs1553149467, rs1553638868, rs1553665866, rs376736188, rs1553642707, rs1553645226, rs1553652883, rs63751435, rs1553653115, rs1553653195, rs63750300, rs1553662622, rs1553658104, rs1553648220, rs1559544064, rs63750584, rs267608083, rs1559551570, rs1559575107, rs1559553501, rs1565986506, rs1559524405, rs1559553492, rs1559554339, rs1559588540, rs1559558071, rs761329565, rs1559521039, rs1559574795, rs1567221417, rs1559578422, rs1481129490, rs1570714352, rs779783209, rs1575376830, rs1575469070, rs1575537843, rs1575620443, rs1575621506, rs587779022, rs1575414904, rs1575449093, rs1575469505, rs1575536254, rs1575537933, rs1575632112, rs1575639851, rs1575441094, rs1575449402, rs267607831, rs2081922847, rs2083403132, rs2085415927, rs2085469647, rs2043913790, rs147542208

Unknown
Disease name	Disease term	dbSNP ID	References
Liver carcinoma	Liver carcinoma		28284560