ENPEP (glutamyl aminopeptidase)

Gene

• Entrez ID: 2028
• Gene name: Glutamyl aminopeptidase
• Gene symbol: ENPEP
• Synonyms: APA, CD249, gp160
• Chromosome: 4
• Chromosome location: 4q25
• Summary: The ENPEP gene encodes glutamyl aminopeptidase, a type II integral membrane protein with an extracellular zinc-binding domain. This protein can upregulate blood pressure by cleaving the N-terminal aspartate from angiotensin II, and can regulate blood vess

miRNA

miRTarBase ID	miRNA	Experiments	Reference
MIRT044621	hsa-miR-320a	CLASH	23622248
MIRT437377	hsa-miR-125b-5p	Luciferase reporter assay, Western blot	24098452
MIRT437377	hsa-miR-125b-5p	Luciferase reporter assay, Western blot	24098452
MIRT963886	hsa-miR-129-5p	CLIP-seq
MIRT963887	hsa-miR-1297	CLIP-seq
MIRT963888	hsa-miR-1299	CLIP-seq
MIRT963889	hsa-miR-133a	CLIP-seq
MIRT963890	hsa-miR-133b	CLIP-seq
MIRT963891	hsa-miR-141	CLIP-seq
MIRT963892	hsa-miR-200a	CLIP-seq
MIRT963893	hsa-miR-2110	CLIP-seq
MIRT963894	hsa-miR-26a	CLIP-seq
MIRT963895	hsa-miR-26b	CLIP-seq
MIRT963896	hsa-miR-3150a-3p	CLIP-seq
MIRT963897	hsa-miR-3159	CLIP-seq
MIRT963898	hsa-miR-4271	CLIP-seq
MIRT963899	hsa-miR-4418	CLIP-seq
MIRT963900	hsa-miR-4465	CLIP-seq
MIRT963901	hsa-miR-4527	CLIP-seq
MIRT963902	hsa-miR-4725-3p	CLIP-seq
MIRT963903	hsa-miR-4726-3p	CLIP-seq
MIRT963904	hsa-miR-4760-3p	CLIP-seq
MIRT963905	hsa-miR-509-3-5p	CLIP-seq
MIRT963906	hsa-miR-509-5p	CLIP-seq
MIRT963907	hsa-miR-513a-3p	CLIP-seq
MIRT963908	hsa-miR-548a-3p	CLIP-seq
MIRT963909	hsa-miR-548e	CLIP-seq
MIRT963910	hsa-miR-548f	CLIP-seq
MIRT963911	hsa-miR-875-3p	CLIP-seq
MIRT963912	hsa-miR-9	CLIP-seq
MIRT1548343	hsa-miR-132	CLIP-seq
MIRT1548344	hsa-miR-212	CLIP-seq
MIRT1548345	hsa-miR-3157-5p	CLIP-seq
MIRT1548346	hsa-miR-4691-3p	CLIP-seq
MIRT1548347	hsa-miR-4709-5p	CLIP-seq
MIRT1985692	hsa-miR-105	CLIP-seq
MIRT1985693	hsa-miR-1295	CLIP-seq
MIRT1985694	hsa-miR-27a	CLIP-seq
MIRT1985695	hsa-miR-27b	CLIP-seq
MIRT1985696	hsa-miR-3150b-3p	CLIP-seq
MIRT1985697	hsa-miR-3658	CLIP-seq
MIRT1985698	hsa-miR-3977	CLIP-seq
MIRT1985699	hsa-miR-4257	CLIP-seq
MIRT1985700	hsa-miR-4662a-3p	CLIP-seq
MIRT1985701	hsa-miR-4717-5p	CLIP-seq
MIRT1985702	hsa-miR-4784	CLIP-seq
MIRT1985703	hsa-miR-513a-5p	CLIP-seq
MIRT1985704	hsa-miR-520d-5p	CLIP-seq
MIRT1985705	hsa-miR-524-5p	CLIP-seq
MIRT2220116	hsa-miR-1200	CLIP-seq
MIRT1985694	hsa-miR-27a	CLIP-seq
MIRT1985695	hsa-miR-27b	CLIP-seq
MIRT2220117	hsa-miR-3074-5p	CLIP-seq
MIRT2220118	hsa-miR-3124-3p	CLIP-seq
MIRT2220119	hsa-miR-3140-5p	CLIP-seq
MIRT2220120	hsa-miR-4753-3p	CLIP-seq
MIRT2220121	hsa-miR-4768-5p	CLIP-seq
MIRT1985703	hsa-miR-513a-5p	CLIP-seq
MIRT2220122	hsa-miR-942	CLIP-seq

Gene ontology (GO)

GO ID	Ontology	Definition	Evidence	Reference
GO:0001525	Process	Angiogenesis	ISS
GO:0002003	Process	Angiotensin maturation	NAS	19608358
GO:0002003	Process	Angiotensin maturation	TAS
GO:0003081	Process	Regulation of systemic arterial blood pressure by renin-angiotensin	IDA	19608358
GO:0003081	Process	Regulation of systemic arterial blood pressure by renin-angiotensin	NAS	8346219
GO:0004177	Function	Aminopeptidase activity	IDA	8244382, 10692253
GO:0005737	Component	Cytoplasm	IBA	21873635
GO:0005765	Component	Lysosomal membrane	HDA	17897319
GO:0005886	Component	Plasma membrane	IBA	21873635
GO:0005886	Component	Plasma membrane	IDA	10692253
GO:0005886	Component	Plasma membrane	TAS
GO:0005887	Component	Integral component of plasma membrane	NAS	8244382
GO:0005903	Component	Brush border	ISS
GO:0006508	Process	Proteolysis	IBA	21873635
GO:0007165	Process	Signal transduction	IBA	21873635
GO:0007267	Process	Cell-cell signaling	NAS	8346219
GO:0008217	Process	Regulation of blood pressure	IBA	21873635
GO:0008270	Function	Zinc ion binding	IBA	21873635
GO:0008283	Process	Cell population proliferation	IDA	14998491
GO:0009897	Component	External side of plasma membrane	ISS
GO:0016324	Component	Apical plasma membrane	ISS
GO:0016477	Process	Cell migration	IDA	14998491
GO:0031410	Component	Cytoplasmic vesicle	ISS
GO:0032835	Process	Glomerulus development	ISS
GO:0042277	Function	Peptide binding	IBA	21873635
GO:0043171	Process	Peptide catabolic process	IBA	21873635
GO:0045177	Component	Apical part of cell	ISS
GO:0070006	Function	Metalloaminopeptidase activity	IBA	21873635
GO:0070006	Function	Metalloaminopeptidase activity	IDA	8346219, 23888046
GO:0070006	Function	Metalloaminopeptidase activity	NAS	8244382
GO:0070062	Component	Extracellular exosome	HDA	19056867, 23533145

Other IDs

• MIM: 138297
• HGNC: 3355
• e!Ensembl: ENSG00000138792

Protein

• UniProt ID: Q07075
• Protein name: Glutamyl aminopeptidase (EAP) (EC 3.4.11.7) (Aminopeptidase A) (AP-A) (Differentiation antigen gp160) (CD antigen CD249)
• Protein function: Regulates central hypertension through its calcium-modulated preference to cleave N-terminal acidic residues from peptides such as angiotensin II.
• PDB: 4KX7, 4KX8, 4KX9, 4KXA, 4KXB, 4KXC, 4KXD
• Family and domains:

  Pfam

  Accession	ID	Position in sequence	Description	Type
  PF17900	Peptidase_M1_N	98 → 286		Domain
  PF01433	Peptidase_M1	321 → 538	Peptidase family M1 domain	Domain
  PF11838	ERAP1_C	616 → 934	ERAP1-like C-terminal domain	Domain

• Sequence:

  MNFAEREGSKRYCIQTKHVAILCAVVVGVGLIVGLAVGLTRSCDSSGDGGPGTAPAPSHL
  PSSTASPSGPPAQDQDICPASEDESGQWKNFRLPDFVNPVHYDLHVKPLLEEDTYTGTVS
  ISINLSAPTRYLWLHLRETRITRLPELKRPSGDQVQVRRCFEYKKQEYVVVEAEEELTPS
  SGDGLYLLTMEFAGWLNGSLVGFYRTTYTENGQVKSIVATDHEPTDARKSFPCFDEPNKK
  ATYTISITHPKEYGALSNMPVAKEESVDDKWTRTTFEKSVPMSTYLVCFAVHQFDSVKRI
  SNSGKPLTIYVQPEQKHTAEYAANITKSVFDYFEEYFAMNYSLPKLDKIAIPDFGTGAME
  NWGLITYRETNLLYDPKESASSNQQRVATVVAHELVHQWFGNIVTMDWWEDLWLNEGFAS
  FFEFLGVNHAETDWQMRDQMLLEDVLPVQEDDSLMSSHPIIVTVTTPDEITSVFDGISYS
  KGSSILRMLEDWIKPENFQKGCQMYLEKYQFKNAKTSDFWAALEEASRLPVKEVMDTWTR
  QMGYPVLNVNGVKNITQKRFLLDPRANPSQPPSDLGYTWNIPVKWTEDNITSSVLFNRSE
  KEGITLNSSNPSGNAFLKINPDHIGFYRVNYEVATWDSIATALSLNHKTFSSADRASLID
  DAFALARAQLLDYKVALNLTKYLKREENFLPWQRVISAVTYIISMFEDDKELYPMIEEYF
  QGQVKPIADSLGWNDAGDHVTKLLRSSVLGFACKMGDREALNNASSLFEQWLNGTVSLPV
  NLRLLVYRYGMQNSGNEISWNYTLEQYQKTSLAQEKEKLLYGLASVKNVTLLSRYLDLLK
  DTNLIKTQDVFTVIRYISYNSYGKNMAWNWIQLNWDYLVNRYTLNNRNLGRIVTIAEPFN
  TELQLWQMESFFAKYPQAGAGEKPREQVLETVKNNIEWLKQHRNTIREWFFNLLESG

• Sequence length: 957

Pathways

KEGG:

Renin-angiotensin system

Reactome:

Metabolism of Angiotensinogen to Angiotensins

Associated diseases

Causal
Disease name	Disease term	dbSNP ID	References
Breast cancer	Malignant neoplasm of breast	rs587776547, rs1137887, rs137853007, rs587776650, rs80359351, rs80359714, rs121917783, rs104886456, rs121964878, rs80359874, rs80357868, rs80357508, rs387906843, rs80357569, rs80358158, rs80357524, rs80357115, rs80357945, rs80357729, rs80357609, rs80357259, rs80357981, rs80358063, rs80357389, rs80356862, rs80359876, rs80357580, rs80358053, rs80358089, rs80187739, rs397507241, rs80358069, rs80357590, rs80357284, rs80357941, rs80359261, rs80359272, rs80359276, rs276174813, rs80358474, rs80359316, rs1555282969, rs80359388, rs80359499, rs80359505, rs80359520, rs80359526, rs80359533, rs56253082, rs80358824, rs80359554, rs80359636, rs80359651, rs80359659, rs80359011, rs80359012, rs80359013, rs80359718, rs397507410, rs81002812, rs80359730, rs80359152, rs80359159, rs397507419, rs28897759, rs80359211, rs80359775, rs397514577, rs397507584, rs80358435, rs80358456, rs80359340, rs80359343, rs80359365, rs80358579, rs397507670, rs80358593, rs80359406, rs80359444, rs80359454, rs276174853, rs276174854, rs80359483, rs80359537, rs80358815, rs80358843, rs80359558, rs80359560, rs80359594, rs80358893, rs28897743, rs397507900, rs397507906, rs397507918, rs80358971, rs80358981, rs397507941, rs80359030, rs80359035, rs41293511, rs397507396, rs81002806, rs80359112, rs397508006, rs81002893, rs45580035, rs80359760, rs397508051, rs80359772, rs4987049, rs80359777, rs80357770, rs397508867, rs62625303, rs397508874, rs80357506, rs80357287, rs273898674, rs80358042, rs80358083, rs80357058, rs41286296, rs80357960, rs80356945, rs80357223, rs386134270, rs80358116, rs80357856, rs80357424, rs397509050, rs80357485, rs80357966, rs397509067, rs80357310, rs80356866, rs80357260, rs80357437, rs80358023, rs80358086, rs80357133, rs80356993, rs80357997, rs80357239, rs80357227, rs397509243, rs80356969, rs80356959, rs63750617, rs63751319, rs587779315, rs200640585, rs398122546, rs80357543, rs398122687, rs80359328, rs398122779, rs398122783, rs62517194, rs80358029, rs515726060, rs180177103, rs180177111, rs180177133, rs587776527, rs180177135, rs180177136, rs515726117, rs587779813, rs587779909, rs587780024, rs587780100, rs28909982, rs121908698, rs180177100, rs587780210, rs587780240, rs587780639, rs587781269, rs587781353, rs587781471, rs587781658, rs587781697, rs587781730, rs587781894, rs587781948, rs587782005, rs587782011, rs200928781, rs587781558, rs370228071, rs587782245, rs587782401, rs180177110, rs587782504, rs72552322, rs587782531, rs587782620, rs587782680, rs587782774, rs587782818, rs730881411, rs730881389, rs564652222, rs397507768, rs587776419, rs730881868, rs730881940, rs56383036, rs758972589, rs201089102, rs730881348, rs786202608, rs786201886, rs786203318, rs786203775, rs786203714, rs786202033, rs750621215, rs786203884, rs786203650, rs772821016, rs863224521, rs864622223, rs864622655, rs375699023, rs876659572, rs768362387, rs876659535, rs876658957, rs483353072, rs876659435, rs267608041, rs876661113, rs730881369, rs878853535, rs772228129, rs878855122, rs760551339, rs80359596, rs397509222, rs886039630, rs886039683, rs886040828, rs587781799, rs886040374, rs886040649, rs397507967, rs878854957, rs886040043, rs1057517589, rs1060502769, rs866380588, rs863224765, rs1064793243, rs747563556, rs1555074976, rs1064795885, rs753961188, rs1064794708, rs869312772, rs1064793887, rs1131690820, rs1135401928, rs1135401868, rs1135401859, rs1553370324, rs397507630, rs1555283160, rs1555283251, rs1555283262, rs1555283361, rs1555286298, rs1555288462, rs886040950, rs1555289566, rs776323117, rs80357123, rs1555579627, rs1555580697, rs80358054, rs1555593302, rs1328985852, rs763470424, rs1555139694, rs878854697, rs1555461217, rs1555461765, rs774684620, rs766416564, rs1554558613, rs1305740166, rs1555461460, rs1555461407, rs1555461586, rs1555567202, rs1555607022, rs1555069815, rs1442299125, rs1474786480, rs1555084947, rs1555457867, rs141087784, rs1482641121, rs1564830522, rs1565469955, rs1565503137, rs864622613, rs755263466, rs757679199, rs1593903166, rs1597801649, rs1603293306, rs879253880, rs80358754, rs1597062038, rs45494092, rs1603275367, rs887358871, rs1597091518, rs1966967065, rs1064793049, rs2082872908, rs2085078278, rs2072475243
Hypertension	Hypertensive disease	rs13306026, rs13333226	30487518

Unknown
Disease name	Disease term	dbSNP ID	References
Cardiovascular diseases	Cardiovascular Diseases		30595370
Mental depression	Unipolar Depression, Major Depressive Disorder	rs587778876, rs587778877	23794217