DAPK1 (death associated protein kinase 1)

Gene

• Entrez ID: 1612
• Gene name: Death associated protein kinase 1
• Gene symbol: DAPK1
• Synonyms: DAPK, ROCO3
• Chromosome: 9
• Chromosome location: 9q21.33
• Summary: Death-associated protein kinase 1 is a positive mediator of gamma-interferon induced programmed cell death. DAPK1 encodes a structurally unique 160-kD calmodulin dependent serine-threonine kinase that carries 8 ankyrin repeats and 2 putative P-loop consen

miRNA

miRTarBase ID	miRNA	Experiments	Reference
MIRT004738	hsa-miR-214-3p	Microarray, qRT-PCR	19435428
MIRT004738	hsa-miR-214-3p	Microarray, qRT-PCR	19435428
MIRT006690	hsa-miR-103a-3p	Luciferase reporter assay, Western blot	22593189
MIRT006690	hsa-miR-103a-3p	Luciferase reporter assay, Western blot	22593189
MIRT006691	hsa-miR-107	Luciferase reporter assay, Western blot	22593189
MIRT006691	hsa-miR-107	Luciferase reporter assay, Western blot	22593189
MIRT022627	hsa-miR-124-3p	Microarray	18668037
MIRT548653	hsa-miR-376c-3p	PAR-CLIP	21572407
MIRT548651	hsa-miR-5580-3p	PAR-CLIP	21572407
MIRT548652	hsa-miR-130a-3p	PAR-CLIP	21572407
MIRT548650	hsa-miR-130b-3p	PAR-CLIP	21572407
MIRT548649	hsa-miR-301a-3p	PAR-CLIP	21572407
MIRT548648	hsa-miR-301b-3p	PAR-CLIP	21572407
MIRT548647	hsa-miR-3666	PAR-CLIP	21572407
MIRT548646	hsa-miR-4295	PAR-CLIP	21572407
MIRT548645	hsa-miR-454-3p	PAR-CLIP	21572407
MIRT548644	hsa-miR-5011-5p	PAR-CLIP	21572407
MIRT548643	hsa-miR-4671-3p	PAR-CLIP	21572407
MIRT548642	hsa-miR-190a-3p	PAR-CLIP	21572407
MIRT548641	hsa-miR-448	PAR-CLIP	21572407
MIRT548640	hsa-miR-1277-5p	PAR-CLIP	21572407
MIRT440849	hsa-miR-218-5p	HITS-CLIP	23212916
MIRT440849	hsa-miR-218-5p	HITS-CLIP	23212916
MIRT732795	hsa-miR-26b-5p	Luciferase reporter assay, qRT-PCR, Western blotting	33839982
MIRT548653	hsa-miR-376c-3p	PAR-CLIP	21572407
MIRT548651	hsa-miR-5580-3p	PAR-CLIP	21572407
MIRT548652	hsa-miR-130a-3p	PAR-CLIP	21572407
MIRT548650	hsa-miR-130b-3p	PAR-CLIP	21572407
MIRT548649	hsa-miR-301a-3p	PAR-CLIP	21572407
MIRT548648	hsa-miR-301b-3p	PAR-CLIP	21572407
MIRT548647	hsa-miR-3666	PAR-CLIP	21572407
MIRT548646	hsa-miR-4295	PAR-CLIP	21572407
MIRT548645	hsa-miR-454-3p	PAR-CLIP	21572407
MIRT548644	hsa-miR-5011-5p	PAR-CLIP	21572407
MIRT548643	hsa-miR-4671-3p	PAR-CLIP	21572407
MIRT548642	hsa-miR-190a-3p	PAR-CLIP	21572407
MIRT548641	hsa-miR-448	PAR-CLIP	21572407
MIRT548640	hsa-miR-1277-5p	PAR-CLIP	21572407
MIRT732795	hsa-miR-26b-5p	Luciferase reporter assay, Western blotting, qRT-PCR, Flow cytometry	33839982
MIRT923157	hsa-miR-320e	CLIP-seq
MIRT923158	hsa-miR-329	CLIP-seq
MIRT923159	hsa-miR-362-3p	CLIP-seq
MIRT923160	hsa-miR-3915	CLIP-seq
MIRT923161	hsa-miR-3928	CLIP-seq
MIRT923162	hsa-miR-522	CLIP-seq
MIRT923163	hsa-miR-607	CLIP-seq
MIRT1547851	hsa-miR-1272	CLIP-seq
MIRT1547852	hsa-miR-1273f	CLIP-seq
MIRT1547853	hsa-miR-143	CLIP-seq
MIRT1547854	hsa-miR-4324	CLIP-seq
MIRT1547855	hsa-miR-4708-5p	CLIP-seq
MIRT1547856	hsa-miR-4712-3p	CLIP-seq
MIRT1547857	hsa-miR-4714-3p	CLIP-seq
MIRT1547858	hsa-miR-4770	CLIP-seq
MIRT1547859	hsa-miR-505	CLIP-seq
MIRT1973515	hsa-miR-361-5p	CLIP-seq
MIRT1973516	hsa-miR-3653	CLIP-seq
MIRT1973517	hsa-miR-3977	CLIP-seq
MIRT1547852	hsa-miR-1273f	CLIP-seq
MIRT1547853	hsa-miR-143	CLIP-seq
MIRT1547854	hsa-miR-4324	CLIP-seq
MIRT2209084	hsa-miR-4534	CLIP-seq
MIRT1547855	hsa-miR-4708-5p	CLIP-seq
MIRT1547856	hsa-miR-4712-3p	CLIP-seq
MIRT1547858	hsa-miR-4770	CLIP-seq
MIRT2386723	hsa-miR-544	CLIP-seq
MIRT2512949	hsa-miR-1321	CLIP-seq
MIRT2512950	hsa-miR-3182	CLIP-seq
MIRT2512951	hsa-miR-3607-3p	CLIP-seq
MIRT2512952	hsa-miR-3686	CLIP-seq
MIRT2512953	hsa-miR-4419a	CLIP-seq
MIRT2512954	hsa-miR-4510	CLIP-seq
MIRT2512955	hsa-miR-4739	CLIP-seq
MIRT2512956	hsa-miR-4756-5p	CLIP-seq
MIRT2512957	hsa-miR-548a-3p	CLIP-seq
MIRT2512958	hsa-miR-548ae	CLIP-seq
MIRT2512959	hsa-miR-548aj	CLIP-seq
MIRT2512960	hsa-miR-548am	CLIP-seq
MIRT2512961	hsa-miR-548e	CLIP-seq
MIRT2512962	hsa-miR-548f	CLIP-seq
MIRT2512963	hsa-miR-548g	CLIP-seq
MIRT2512964	hsa-miR-548x	CLIP-seq
MIRT2512965	hsa-miR-570	CLIP-seq
MIRT2512966	hsa-miR-593	CLIP-seq
MIRT2512957	hsa-miR-548a-3p	CLIP-seq
MIRT2512958	hsa-miR-548ae	CLIP-seq
MIRT2512959	hsa-miR-548aj	CLIP-seq
MIRT2512960	hsa-miR-548am	CLIP-seq
MIRT2512961	hsa-miR-548e	CLIP-seq
MIRT2512962	hsa-miR-548f	CLIP-seq
MIRT2512963	hsa-miR-548g	CLIP-seq
MIRT2512964	hsa-miR-548x	CLIP-seq
MIRT2512965	hsa-miR-570	CLIP-seq

Transcription factors

Transcription factor	Regulation	Reference
CEBPB	Unknown	19521987
HOXB7	Repression	17540169
MBD2	Unknown	21693597

Gene ontology (GO)

GO ID	Ontology	Definition	Evidence	Reference
GO:0004672	Function	Protein kinase activity	IDA	7828849, 12730201
GO:0004674	Function	Protein serine/threonine kinase activity	IBA	21873635
GO:0004674	Function	Protein serine/threonine kinase activity	TAS	16756490
GO:0004683	Function	Calmodulin-dependent protein kinase activity	IDA	10629061
GO:0005515	Function	Protein binding	IPI	15616583, 16132846, 19180116, 19712061, 20389280, 21725354, 25241761, 25852190, 27068745, 28169274, 29513927
GO:0005516	Function	Calmodulin binding	IDA	10629061
GO:0005524	Function	ATP binding	IDA	10629061
GO:0005525	Function	GTP binding	IEA
GO:0005634	Component	Nucleus	IBA	21873635
GO:0005737	Component	Cytoplasm	IBA	21873635
GO:0005737	Component	Cytoplasm	IDA	12730201
GO:0005886	Component	Plasma membrane	IDA	12730201
GO:0006468	Process	Protein phosphorylation	IDA	10629061
GO:0006468	Process	Protein phosphorylation	TAS	16756490
GO:0006915	Process	Apoptotic process	IGI	7828849
GO:0006915	Process	Apoptotic process	IMP	10629061
GO:0010506	Process	Regulation of autophagy	TAS	16756490
GO:0010508	Process	Positive regulation of autophagy	IMP	22095288
GO:0015629	Component	Actin cytoskeleton	IDA	10629061
GO:0017075	Function	Syntaxin-1 binding	IPI	12730201
GO:0017148	Process	Negative regulation of translation	IDA	18995835
GO:0035556	Process	Intracellular signal transduction	IBA	21873635
GO:0035556	Process	Intracellular signal transduction	IDA	10629061
GO:0042802	Function	Identical protein binding	IPI	19180116, 19712061, 21738225
GO:0042981	Process	Regulation of apoptotic process	TAS	16756490
GO:0043065	Process	Positive regulation of apoptotic process	IBA	21873635
GO:0043280	Process	Positive regulation of cysteine-type endopeptidase activity involved in apoptotic process	IDA	16132846
GO:0046777	Process	Protein autophosphorylation	IDA	10629061, 12730201
GO:0046777	Process	Protein autophosphorylation	TAS	16756490
GO:0071346	Process	Cellular response to interferon-gamma	IDA	18995835
GO:0071447	Process	Cellular response to hydroperoxide	IMP	22095288
GO:0097190	Process	Apoptotic signaling pathway	IMP	7828849
GO:0106310	Function	Protein serine kinase activity	IEA
GO:0106311	Function	Protein threonine kinase activity	IEA
GO:2000310	Process	Regulation of NMDA receptor activity	ISS

Other IDs

• MIM: 600831
• HGNC: 2674
• e!Ensembl: ENSG00000196730

Protein

• UniProt ID: P53355
• Protein name: Death-associated protein kinase 1 (DAP kinase 1) (EC 2.7.11.1)
• Protein function: Calcium/calmodulin-dependent serine/threonine kinase involved in multiple cellular signaling pathways that trigger cell survival, apoptosis, and autophagy. Regulates both type I apoptotic and type II autophagic cell deaths signal, depending on t
• PDB: 1IG1, 1JKK, 1JKL, 1JKS, 1JKT, 1P4F, 1WVW, 1WVX, 1WVY, 1YR5, 2W4J, 2W4K, 2X0G, 2XUU, 2XZS, 2Y0A, 2Y4P, 2Y4V, 2YAK, 3DFC, 3DGK, 3EH9, 3EHA, 3F5G, 3F5U, 3GU4, 3GU5, 3GU6, 3GU7, 3GU8, 3GUB, 3ZXT, 4B4L, 4PF4, 4TL0, 4TXC, 4UV0, 4YO4, 4YPD, 5AUT, 5AUU, 5AUV, 5AUW, 5AUX, 5AUY, 5AUZ, 5AV0, 5AV1, 5AV2, 5AV3, 5AV4
• Family and domains:

  Pfam

  Accession	ID	Position in sequence	Description	Type
  PF00069	Pkinase	13 → 275	Protein kinase domain	Domain
  PF00023	Ank	444 → 476	Ankyrin repeat	Repeat
  PF00023	Ank	477 → 509	Ankyrin repeat	Repeat
  PF00023	Ank	510 → 542	Ankyrin repeat	Repeat
  PF00023	Ank	576 → 608	Ankyrin repeat	Repeat
  PF00023	Ank	609 → 641	Ankyrin repeat	Repeat
  PF00531	Death	1310 → 1396	Death domain	Domain

• Sequence:

  MTVFRQENVDDYYDTGEELGSGQFAVVKKCREKSTGLQYAAKFIKKRRTKSSRRGVSRED
  IEREVSILKEIQHPNVITLHEVYENKTDVILILELVAGGELFDFLAEKESLTEEEATEFL
  KQILNGVYYLHSLQIAHFDLKPENIMLLDRNVPKPRIKIIDFGLAHKIDFGNEFKNIFGT
  PEFVAPEIVNYEPLGLEADMWSIGVITYILLSGASPFLGDTKQETLANVSAVNYEFEDEY
  FSNTSALAKDFIRRLLVKDPKKRMTIQDSLQHPWIKPKDTQQALSRKASAVNMEKFKKFA
  ARKKWKQSVRLISLCQRLSRSFLSRSNMSVARSDDTLDEEDSFVMKAIIHAINDDNVPGL
  QHLLGSLSNYDVNQPNKHGTPPLLIAAGCGNIQILQLLIKRGSRIDVQDKGGSNAVYWAA
  RHGHVDTLKFLSENKCPLDVKDKSGEMALHVAARYGHADVAQLLCSFGSNPNIQDKEEET
  PLHCAAWHGYYSVAKALCEAGCNVNIKNREGETPLLTASARGYHDIVECLAEHGADLNAC
  DKDGHIALHLAVRRCQMEVIKTLLSQGCFVDYQDRHGNTPLHVACKDGNMPIVVALCEAN
  CNLDISNKYGRTPLHLAANNGILDVVRYLCLMGASVEALTTDGKTAEDLARSEQHEHVAG
  LLARLRKDTHRGLFIQQLRPTQNLQPRIKLKLFGHSGSGKTTLVESLKCGLLRSFFRRRR
  PRLSSTNSSRFPPSPLASKPTVSVSINNLYPGCENVSVRSRSMMFEPGLTKGMLEVFVAP
  THHPHCSADDQSTKAIDIQNAYLNGVGDFSVWEFSGNPVYFCCYDYFAANDPTSIHVVVF
  SLEEPYEIQLNQVIFWLSFLKSLVPVEEPIAFGGKLKNPLQVVLVATHADIMNVPRPAGG
  EFGYDKDTSLLKEIRNRFGNDLHISNKLFVLDAGASGSKDMKVLRNHLQEIRSQIVSVCP
  PMTHLCEKIISTLPSWRKLNGPNQLMSLQQFVYDVQDQLNPLASEEDLRRIAQQLHSTGE
  INIMQSETVQDVLLLDPRWLCTNVLGKLLSVETPRALHHYRGRYTVEDIQRLVPDSDVEE
  LLQILDAMDICARDLSSGTMVDVPALIKTDNLHRSWADEEDEVMVYGGVRIVPVEHLTPF
  PCGIFHKVQVNLCRWIHQQSTEGDADIRLWVNGCKLANRGAELLVLLVNHGQGIEVQVRG
  LETEKIKCCLLLDSVCSTIENVMATTLPGLLTVKHYLSPQQLREHHEPVMIYQPRDFFRA
  QTLKETSLTNTMGGYKESFSSIMCFGCHDVYSQASLGMDIHASDLNLLTRRKLSRLLDPP
  DPLGKDWCLLAMNLGLPDLVAKYNTSNGAPKDFLPSPLHALLREWTTYPESTVGTLMSKL
  RELGRRDAADFLLKASSVFKINLDGNGQEAYASSCNSGTSYNSISSVVSR

• Sequence length: 1430

Pathways

KEGG:

Autophagy - animal
Pathways in cancer
Bladder cancer

Associated diseases

Causal
Disease name	Disease term	dbSNP ID	References
Adenocarcinoma	Adenocarcinoma, Adenocarcinoma, Basal Cell, Adenocarcinoma, Oxyphilic, Adenocarcinoma, Tubular, Adenoid Cystic Carcinoma	rs121913530, rs886039394, rs121913474	15172992, 16762588
Arthritis	Juvenile arthritis	rs1594890601, rs1594882933, rs184370809, rs776489319, rs1594883470	19565504
Carcinoma	Carcinoma, Cribriform, Carcinoma, Granular Cell	rs121912654, rs555607708, rs786202962, rs1564055259	15172992
Leukemia	Leukemia, Myelocytic, Acute, Acute Myeloid Leukemia (AML-M2)	rs121909646, rs121913488, rs587776834, rs752746786, rs869312821, rs767454740, rs1554564297, rs11978267, rs4132601	19874806
Lung carcinoma	Non-Small Cell Lung Carcinoma	rs1805076, rs121909071, rs121913530, rs112445441, rs121913529, rs121913535, rs121913297, rs121913279, rs104886003, rs397516975, rs11554290, rs121913364, rs121913351, rs121913369, rs121913355, rs121912470, rs121913273, rs121913281, rs121913348, rs727503093, rs121913353, rs397516890, rs397516896, rs121913378, rs397516897, rs397516977, rs397516978, rs397516979, rs397516980, rs397516981, rs397516982, rs121913240, rs17851045, rs397517086, rs121913428, rs397517094, rs397517098, rs397517106, rs121913465, rs397517108, rs397517111, rs397517112, rs397517114, rs397517116, rs1554350366, rs397517127, rs397517200, rs397517202, rs121913283, rs121913370, rs121913357, rs727503106, rs121913238, rs727503108, rs397517040, rs397516976, rs1555618025, rs1057519729, rs1584238193	11313923
Lung cancer	Malignant neoplasm of lung	rs121913530, rs121913529, rs878855122, rs1057519784, rs770315135	15172992
Myelodysplastic syndrome	MYELODYSPLASTIC SYNDROME	rs193303018, rs387906631, rs1576745225, rs373145711, rs752746786, rs377023736, rs373221034, rs1576749014, rs1600586587	19874806
Papillary renal carcinoma	Papillary Renal Cell Carcinoma	rs5030823, rs2137087134, rs121913668, rs121913669, rs121913670, rs121913671, rs121913673, rs121913243, rs786202724	17133271
Renal carcinoma	Renal Cell Carcinoma, Conventional (Clear Cell) Renal Cell Carcinoma, Sarcomatoid Renal Cell Carcinoma, Collecting Duct Carcinoma of the Kidney	rs121913668, rs121913670, rs121913243, rs786202724	17133271

Unknown
Disease name	Disease term	dbSNP ID	References
Brain neoplasms	Brain Neoplasms, Malignant neoplasm of brain, Benign neoplasm of brain, unspecified, Brain Tumor, Primary, Recurrent Brain Neoplasm, Primary malignant neoplasm of brain	rs1800720, rs587781720	17319784
Intracranial neoplasm	Neoplasms, Intracranial		17319784
Chromophobe carcinoma	Chromophobe Renal Cell Carcinoma	rs137853247	17133271
Conjunctival diseases	Conjunctival Diseases		23872714
Juvenile arthritis	Juvenile psoriatic arthritis		19565504
Lung neoplasms	Lung Neoplasms		15172992
Myeloid leukemia	Acute Myeloid Leukemia, M1		19874806
Nervous system diseases	Peripheral Nervous System Diseases		23872714
Pancreatic carcinoma	Pancreatic carcinoma		22293537
Seronegative polyarthritis	Polyarthritis, Juvenile, Rheumatoid Factor Negative		19565504
Polyarthritis, rheumatoid factor positive	Polyarthritis, Juvenile, Rheumatoid Factor Positive		19565504
Respiratory tract diseases	Respiratory Tract Diseases		23872714
Salivary gland neoplasm	Salivary Gland Neoplasms		16762588
Malignant neoplasm of salivary gland	Malignant neoplasm of salivary gland		16762588
Still disease	Juvenile-Onset Still Disease		19565504
Treatment related secondary malignancy	Treatment related secondary malignancy		19874806