PIWIL4 (piwi like RNA-mediated gene silencing 4)

Gene

• Entrez ID: 143689
• Gene name: Piwi like RNA-mediated gene silencing 4
• Gene symbol: PIWIL4
• Synonyms: HIWI2, MIWI2
• Chromosome: 11
• Chromosome location: 11q21
• Summary: PIWIL4 belongs to the Argonaute family of proteins, which function in development and maintenance of germline stem cells (Sasaki et al., 2003 [PubMed 12906857]).[supplied by OMIM, Mar 2008]

miRNA

miRTarBase ID	miRNA	Experiments	Reference
MIRT018636	hsa-miR-335-5p	Microarray	18185580
MIRT023027	hsa-miR-124-3p	Microarray	18668037
MIRT437516	hsa-miR-19b-3p	Immunoprecipitaion	22382630
MIRT1237076	hsa-miR-1283	CLIP-seq
MIRT1237077	hsa-miR-382	CLIP-seq
MIRT1237078	hsa-miR-3942-3p	CLIP-seq
MIRT1237079	hsa-miR-452	CLIP-seq
MIRT1237080	hsa-miR-4676-3p	CLIP-seq
MIRT1237081	hsa-miR-4698	CLIP-seq
MIRT1237082	hsa-miR-548a-3p	CLIP-seq
MIRT1237083	hsa-miR-548e	CLIP-seq
MIRT1237084	hsa-miR-548f	CLIP-seq
MIRT1237085	hsa-miR-548g	CLIP-seq

Gene ontology (GO)

GO ID	Ontology	Definition	Evidence	Reference
GO:0004521	Function	Endoribonuclease activity	ISS
GO:0005634	Component	Nucleus	IDA	28025795
GO:0005634	Component	Nucleus	ISS
GO:0005737	Component	Cytoplasm	IDA	28025795
GO:0005737	Component	Cytoplasm	ISS
GO:0006417	Process	Regulation of translation	IEA
GO:0007275	Process	Multicellular organism development	IEA
GO:0007283	Process	Spermatogenesis	ISS
GO:0010529	Process	Negative regulation of transposition	ISS
GO:0010669	Process	Epithelial structure maintenance	IMP	28025795
GO:0030154	Process	Cell differentiation	IEA
GO:0031047	Process	Gene silencing by RNA	ISS
GO:0034584	Function	PiRNA binding	IDA	25038252
GO:0034584	Function	PiRNA binding	ISS
GO:0034587	Process	PiRNA metabolic process	ISS
GO:0043046	Process	DNA methylation involved in gamete generation	ISS
GO:0043186	Component	P granule	ISS
GO:0051321	Process	Meiotic cell cycle	IEA
GO:0071547	Component	PiP-body	ISS
GO:0090502	Process	RNA phosphodiester bond hydrolysis, endonucleolytic	IEA

Other IDs

• MIM: 610315
• HGNC: 18444
• e!Ensembl: ENSG00000134627

Protein

• UniProt ID: Q7Z3Z4
• Protein name: Piwi-like protein 4
• Protein function: Plays a central role during spermatogenesis by repressing transposable elements and preventing their mobilization, which is essential for the germline integrity (By similarity). Acts via the piRNA metabolic process, which mediates the repression
• Family and domains:

  Pfam

  Accession	ID	Position in sequence	Description	Type
  PF02170	PAZ	274 → 406	PAZ domain	Domain
  PF02171	Piwi	546 → 838	Piwi domain	Family

• Sequence:

  MSGRARVKARGIARSPSATEVGRIQASPLPRSVDLSNNEASSSNGFLGTSRISTNDKYGI
  SSGDAGSTFMERGVKNKQDFMDLSICTREKLAHVRNCKTGSSGIPVKLVTNLFNLDFPQD
  WQLYQYHVTYIPDLASRRLRIALLYSHSELSNKAKAFDGAILFLSQKLEEKVTELSSETQ
  RGETIKMTITLKRELPSSSPVCIQVFNIIFRKILKKLSMYQIGRNFYNPSEPMEIPQHKL
  SLWPGFAISVSYFERKLLFSADVSYKVLRNETVLEFMTALCQRTGLSCFTQTCEKQLIGL
  IVLTRYNNRTYSIDDIDWSVKPTHTFQKRDGTEITYVDYYKQQYDITVSDLNQPMLVSLL
  KKKRNDNSEAQLAHLIPELCFLTGLTDQATSDFQLMKAVAEKTRLSPSGRQQRLARLVDN
  IQRNTNARFELETWGLHFGSQISLTGRIVPSEKILMQDHICQPVSAADWSKDIRTCKILN
  AQSLNTWLILCSDRTEYVAESFLNCLRRVAGSMGFNVDYPKIIKVQENPAAFVRAIQQYV
  DPDVQLVMCILPSNQKTYYDSIKKYLSSDCPVPSQCVLARTLNKQGMMMSIATKIAMQMT
  CKLGGELWAVEIPLKSLMVVGIDVCKDALSKDVMVVGCVASVNPRITRWFSRCILQRTMT
  DVADCLKVFMTGALNKWYKYNHDLPARIIVYRAGVGDGQLKTLIEYEVPQLLSSVAESSS
  NTSSRLSVIVVRKKCMPRFFTEMNRTVQNPPLGTVVDSEATRNEWYDFYLISQVACRGTV
  SPTYYNVIYDDNGLKPDHMQRLTFKLCHLYYNWPGIVSVPAPCQYAHKLTFLVAQSIHKE
  PSLELANHLFYL

• Sequence length: 852

Associated diseases

Causal
Disease name	Disease term	dbSNP ID	References
Attention deficit hyperactivity disorder	Attention deficit hyperactivity disorder	rs120074176, rs786205019	18846501
Oligospermia	Oligospermia	rs1602125411, rs2047796277, rs377712900	20940137