THOP1 (thimet oligopeptidase 1)

Gene

• Entrez ID: 7064
• Gene name: Thimet oligopeptidase 1
• Gene symbol: THOP1
• Synonyms (NCBI Gene): EP24.15, MEPD_HUMAN, MP78, TOP
• Chromosome: 19
• Chromosome location: 19p13.3
• Summary: The protein encoded by this gene is a kininase that uses zinc as a cofactor. The encoded oligopeptidase cleaves cytosolic peptides, making them unavailable for display on antigen-presenting cells. This protein also cleaves neuropeptides under 20 aa in len

miRNA

miRTarBase ID	miRNA	Experiments	Reference
MIRT020122	hsa-miR-130b-3p	Sequencing	20371350
MIRT024285	hsa-miR-215-5p	Microarray	19074876
MIRT026106	hsa-miR-192-5p	Microarray	19074876
MIRT030367	hsa-miR-24-3p	Microarray	19748357
MIRT031093	hsa-miR-19b-3p	Sequencing	20371350
MIRT046579	hsa-miR-222-3p	CLASH	23622248
MIRT041826	hsa-miR-484	CLASH	23622248
MIRT041034	hsa-miR-505-3p	CLASH	23622248
MIRT040074	hsa-miR-615-3p	CLASH	23622248
MIRT039328	hsa-miR-425-5p	CLASH	23622248
MIRT036680	hsa-miR-935	CLASH	23622248
MIRT1423532	hsa-miR-130a	CLIP-seq
MIRT1423533	hsa-miR-130b	CLIP-seq
MIRT1423534	hsa-miR-1915	CLIP-seq
MIRT1423535	hsa-miR-301a	CLIP-seq
MIRT1423536	hsa-miR-301b	CLIP-seq
MIRT1423537	hsa-miR-3065-5p	CLIP-seq
MIRT1423538	hsa-miR-3152-3p	CLIP-seq
MIRT1423539	hsa-miR-3157-5p	CLIP-seq
MIRT1423540	hsa-miR-3620	CLIP-seq
MIRT1423541	hsa-miR-3666	CLIP-seq
MIRT1423542	hsa-miR-4269	CLIP-seq
MIRT1423543	hsa-miR-4295	CLIP-seq
MIRT1423544	hsa-miR-4473	CLIP-seq
MIRT1423545	hsa-miR-454	CLIP-seq
MIRT1423546	hsa-miR-4645-5p	CLIP-seq
MIRT1423547	hsa-miR-4671-3p	CLIP-seq
MIRT1423548	hsa-miR-4673	CLIP-seq
MIRT1423549	hsa-miR-4691-3p	CLIP-seq
MIRT1423550	hsa-miR-4722-3p	CLIP-seq
MIRT1423551	hsa-miR-4726-3p	CLIP-seq
MIRT1423549	hsa-miR-4691-3p	CLIP-seq
MIRT2461444	hsa-miR-1291	CLIP-seq
MIRT1423532	hsa-miR-130a	CLIP-seq
MIRT1423533	hsa-miR-130b	CLIP-seq
MIRT1423535	hsa-miR-301a	CLIP-seq
MIRT1423536	hsa-miR-301b	CLIP-seq
MIRT1423537	hsa-miR-3065-5p	CLIP-seq
MIRT1423541	hsa-miR-3666	CLIP-seq
MIRT1423543	hsa-miR-4295	CLIP-seq
MIRT1423544	hsa-miR-4473	CLIP-seq
MIRT1423545	hsa-miR-454	CLIP-seq
MIRT1423547	hsa-miR-4671-3p	CLIP-seq

Gene ontology (GO)

GO ID	Ontology	Definition	Evidence	Reference
GO:0000209	Process	Protein polyubiquitination	TAS
GO:0004222	Function	Metalloendopeptidase activity	IBA	21873635
GO:0004222	Function	Metalloendopeptidase activity	TAS
GO:0005515	Function	Protein binding	IPI	32296183, 32814053
GO:0005758	Component	Mitochondrial intermembrane space	IBA	21873635
GO:0005829	Component	Cytosol	TAS
GO:0006508	Process	Proteolysis	IBA	21873635
GO:0006518	Process	Peptide metabolic process	IBA	21873635
GO:0042277	Function	Peptide binding	IEA
GO:0046872	Function	Metal ion binding	IEA

Other IDs

• MIM: 601117
• HGNC: 11793
• e!Ensembl: ENSG00000172009

Protein

• UniProt ID: P52888
• Protein name: Thimet oligopeptidase (EC 3.4.24.15) (Endopeptidase 24.15) (MP78)
• Protein function: Involved in the metabolism of neuropeptides under 20 amino acid residues long. Involved in cytoplasmic peptide degradation (PubMed:17251185, PubMed:7639763). Able to degrade the amyloid-beta precursor protein and generate amyloidogenic fragments
• PDB: 1S4B, 2O36
• Family and domains:

  Pfam

  Accession	ID	Position in sequence	Description	Type
  PF01432	Peptidase_M3	227 → 677	Peptidase family M3	Family

• Sequence:

  MKPPAACAGDMADAASPCSVVNDLRWDLSAQQIEERTRELIEQTKRVYDQVGTQEFEDVS
  YESTLKALADVEVTYTVQRNILDFPQHVSPSKDIRTASTEADKKLSEFDVEMSMREDVYQ
  RIVWLQEKVQKDSLRPEAARYLERLIKLGRRNGLHLPRETQENIKRIKKKLSLLCIDFNK
  NLNEDTTFLPFTLQELGGLPEDFLNSLEKMEDGKLKVTLKYPHYFPLLKKCHVPETRRKV
  EEAFNCRCKEENCAILKELVTLRAQKSRLLGFHTHADYVLEMNMAKTSQTVATFLDELAQ
  KLKPLGEQERAVILELKRAECERRGLPFDGRIRAWDMRYYMNQVEETRYCVDQNLLKEYF
  PVQVVTHGLLGIYQELLGLAFHHEEGASAWHEDVRLYTARDAASGEVVGKFYLDLYPREG
  KYGHAACFGLQPGCLRQDGSRQIAIAAMVANFTKPTADAPSLLQHDEVETYFHEFGHVMH
  QLCSQAEFAMFSGTHVERDFVEAPSQMLENWVWEQEPLLRMSRHYRTGSAVPRELLEKLI
  ESRQANTGLFNLRQIVLAKVDQALHTQTDADPAEEYARLCQEILGVPATPGTNMPATFGH
  LAGGYDAQYYGYLWSEVYSMDMFHTRFKQEGVLNSKVGMDYRSCILRPGGSEDASAMLRR
  FLGRDPKQDAFLLSKGLQVGGCEPEPQVC

• Sequence length: 689