RAPGEF4 (Rap guanine nucleotide exchange factor 4)

Gene

• Entrez ID: 11069
• Gene name: Rap guanine nucleotide exchange factor 4
• Gene symbol: RAPGEF4
• Synonyms: CAMP-GEFII, CGEF2, EPAC, EPAC 2, EPAC2, Nbla00496
• Chromosome: 2
• Chromosome location: 2q31.1

miRNA

miRTarBase ID	miRNA	Experiments	Reference
MIRT018141	hsa-miR-335-5p	Microarray	18185580
MIRT027613	hsa-miR-98-5p	Microarray	19088304
MIRT029706	hsa-miR-26b-5p	Microarray	19088304
MIRT156401	hsa-miR-17-5p	HITS-CLIP	22473208
MIRT156402	hsa-miR-19a-3p	HITS-CLIP	22473208
MIRT156403	hsa-miR-19b-3p	HITS-CLIP	22473208
MIRT156404	hsa-miR-20a-5p	HITS-CLIP	22473208
MIRT156406	hsa-miR-93-5p	HITS-CLIP	22473208
MIRT156411	hsa-miR-106b-5p	HITS-CLIP	22473208
MIRT156417	hsa-miR-20b-5p	HITS-CLIP	22473208
MIRT156424	hsa-miR-519d-3p	HITS-CLIP	22473208

Gene ontology (GO)

GO ID	Ontology	Definition	Evidence	Reference
GO:0005085	Function	Guanyl-nucleotide exchange factor activity	ISS
GO:0005515	Function	Protein binding	IPI	11438518, 24722188, 25416956, 32296183, 32814053
GO:0005829	Component	Cytosol	IEA
GO:0005886	Component	Plasma membrane	TAS
GO:0007186	Process	G protein-coupled receptor signaling pathway	TAS	9856955
GO:0007264	Process	Small GTPase mediated signal transduction	IEA
GO:0016020	Component	Membrane	TAS	10777494
GO:0017156	Process	Calcium-ion regulated exocytosis	IEA
GO:0017157	Process	Regulation of exocytosis	IEA
GO:0019933	Process	CAMP-mediated signaling	IEA
GO:0030073	Process	Insulin secretion	IEA
GO:0030552	Function	CAMP binding	ISS
GO:0031267	Function	Small GTPase binding	ISS
GO:0050790	Process	Regulation of catalytic activity	IEA
GO:0050796	Process	Regulation of insulin secretion	TAS
GO:0098686	Component	Hippocampal mossy fiber to CA3 synapse	IEA
GO:0098693	Process	Regulation of synaptic vesicle cycle	IEA

Other IDs

• MIM: 606058
• HGNC: 16626
• e!Ensembl: ENSG00000091428

Protein

• UniProt ID: Q8WZA2
• Protein name: Rap guanine nucleotide exchange factor 4 (Exchange factor directly activated by cAMP 2) (Exchange protein directly activated by cAMP 2) (EPAC 2) (cAMP-regulated guanine nucleotide exchange factor II) (cAMP-GEFII)
• Protein function: Guanine nucleotide exchange factor (GEF) for RAP1A, RAP1B and RAP2A small GTPases that is activated by binding cAMP. Seems not to activate RAB3A. Involved in cAMP-dependent, PKA-independent exocytosis through interaction with RIMS2 (By similarity).
• Family and domains:

  Pfam

  Accession	ID	Position in sequence	Description	Type
  PF00027	cNMP_binding	61 → 150	Cyclic nucleotide-binding domain	Domain
  PF00610	DEP	220 → 289	Domain found in Dishevelled, Egl-10, and Pleckstrin (DEP)	Domain
  PF00027	cNMP_binding	375 → 459	Cyclic nucleotide-binding domain	Domain
  PF00618	RasGEF_N	499 → 605	RasGEF N-terminal motif	Domain
  PF00617	RasGEF	775 → 952	RasGEF domain	Family

• Sequence:

  MVAAHAAHSSSSAEWIACLDKRPLERSSEDVDIIFTRLKEVKAFEKFHPNLLHQICLCGY
  YENLEKGITLFRQGDIGTNWYAVLAGSLDVKVSETSSHQDAVTICTLGIGTAFGESILDN
  TPRHATIVTRESSELLRIEQKDFKALWEKYRQYMAGLLAPPYGVMETGSNNDRIPDKENT
  PLIEPHVPLRPANTITKVPSEKILRAGKILRNAILSRAPHMIRDRKYHLKTYRQCCVGTE
  LVDWMMQQTPCVHSRTQAVGMWQVLLEDGVLNHVDQEHHFQDKYLFYRFLDDEHEDAPLP
  TEEEKKECDEELQDTMLLLSQMGPDAHMRMILRKPPGQRTVDDLEIIYEELLHIKALSHL
  STTVKRELAGVLIFESHAKGGTVLFNQGEEGTSWYIILKGSVNVVIYGKGVVCTLHEGDD
  FGKLALVNDAPRAASIVLREDNCHFLRVDKEDFNRILRDVEANTVRLKEHDQDVLVLEKV
  PAGNRASNQGNSQPQQKYTVMSGTPEKILEHFLETIRLEATLNEATDSVLNDFIMMHCVF
  MPNTQLCPALVAHYHAQPSQGTEQEKMDYALNNKRRVIRLVLQWAAMYGDLLQEDDVSMA
  FLEEFYVSVSDDARMIAALKEQLPELEKIVKQISEDAKAPQKKHKVLLQQFNTGDERAQK
  RQPIRGSDEVLFKVYCMDHTYTTIRVPVATSVKEVISAVADKLGSGEGLIIVKMSSGGEK
  VVLKPNDVSVFTTLTINGRLFACPREQFDSLTPLPEQEGPTVGTVGTFELMSSKDLAYQM
  TIYDWELFNCVHELELIYHTFGRHNFKKTTANLDLFLRRFNEIQFWVVTEICLCSQLSKR
  VQLLKKFIKIAAHCKEYKNLNSFFAIVMGLSNVAVSRLALTWEKLPSKFKKFYAEFESLM
  DPSRNHRAYRLTVAKLEPPLIPFMPLLIKDMTFTHEGNKTFIDNLVNFEKMRMIANTART
  VRYYRSQPFNPDAAQANKNHQDVRSYVRQLNVIDNQRTLSQMSHRLEPRRP

• Sequence length: 1011