RAP1A (RAP1A, member of RAS oncogene family)

Gene

• Entrez ID: 5906
• Gene name: RAP1A, member of RAS oncogene family
• Gene symbol: RAP1A
• Synonyms: C21KG, G-22K, KREV-1, KREV1, RAP1, SMGP21
• Chromosome: 1
• Chromosome location: 1p13.2
• Summary: This gene encodes a member of the Ras family of small GTPases. The encoded protein undergoes a change in conformational state and activity, depending on whether it is bound to GTP or GDP. This protein is activated by several types of guanine nucleotide exchange factors (GEFs), and inactivated by two groups of GTPase-activating proteins (GAPs). The activation status of the encoded protein is therefore affected by the balance of intracellular levels of GEFs and GAPs. The encoded protein regulates signaling pathways that affect cell proliferation and adhesion, and may play a role in tumor malignancy. Pseudogenes of this gene have been defined on chromosomes 14 and 17. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]

miRNA

miRTarBase ID	miRNA	Experiments	Reference
MIRT006958	hsa-miR-337-3p	Luciferase reporter assay, qRT-PCR, Western blot	22723956
MIRT051421	hsa-let-7e-5p	CLASH	23622248
MIRT448551	hsa-miR-3591-5p	PAR-CLIP	22100165
MIRT448552	hsa-miR-1273e	PAR-CLIP	22100165
MIRT448553	hsa-miR-4473	PAR-CLIP	22100165
MIRT448554	hsa-miR-4671-3p	PAR-CLIP	22100165
MIRT448555	hsa-miR-636	PAR-CLIP	22100165
MIRT448556	hsa-miR-135a-3p	PAR-CLIP	22100165
MIRT448557	hsa-miR-302c-3p	PAR-CLIP	22100165
MIRT448558	hsa-miR-512-3p	PAR-CLIP	22100165
MIRT448559	hsa-miR-520f-3p	PAR-CLIP	22100165
MIRT512891	hsa-miR-4470	PAR-CLIP	23446348
MIRT512892	hsa-miR-759	PAR-CLIP	23446348
MIRT512893	hsa-miR-4761-5p	PAR-CLIP	23446348
MIRT512894	hsa-miR-5000-5p	PAR-CLIP	23446348
MIRT512895	hsa-miR-4735-3p	PAR-CLIP	23446348
MIRT512896	hsa-miR-18b-5p	PAR-CLIP	23446348
MIRT512897	hsa-miR-18a-5p	PAR-CLIP	23446348
MIRT512898	hsa-miR-551b-5p	PAR-CLIP	23446348
MIRT554934	hsa-miR-4799-5p	PAR-CLIP	21572407
MIRT554935	hsa-miR-8059	PAR-CLIP	21572407
MIRT554936	hsa-miR-4471	PAR-CLIP	21572407
MIRT554937	hsa-miR-6837-3p	PAR-CLIP	21572407
MIRT554938	hsa-miR-6754-3p	PAR-CLIP	21572407
MIRT554939	hsa-miR-412-3p	PAR-CLIP	21572407
MIRT554940	hsa-miR-6715a-3p	PAR-CLIP	21572407
MIRT554941	hsa-miR-5692c	PAR-CLIP	21572407
MIRT554942	hsa-miR-5692b	PAR-CLIP	21572407
MIRT554943	hsa-miR-6822-5p	PAR-CLIP	21572407
MIRT554944	hsa-miR-6750-5p	PAR-CLIP	21572407
MIRT554945	hsa-miR-1277-5p	PAR-CLIP	21572407
MIRT554946	hsa-miR-10a-3p	PAR-CLIP	21572407
MIRT554947	hsa-miR-19a-3p	HITS-CLIP	22473208
MIRT554947	hsa-miR-19a-3p	PAR-CLIP	21572407
MIRT554948	hsa-miR-19b-3p	HITS-CLIP	22473208
MIRT554948	hsa-miR-19b-3p	PAR-CLIP	21572407
MIRT734682	hsa-miR-203a-3p	Luciferase reporter assay, Microarray, qRT-PCR, Western blot	27780730
MIRT734748	hsa-miR-203a-5p	Luciferase reporter assay, qRT-PCR, Western blot	25636908
MIRT761765	hsa-miR-1268a	PAR-CLIP	27292025
MIRT761767	hsa-miR-1268b	PAR-CLIP	27292025
MIRT761784	hsa-miR-1272	PAR-CLIP	27292025
MIRT765735	hsa-miR-340-3p	PAR-CLIP	27292025
MIRT769639	hsa-miR-4486	PAR-CLIP	27292025
MIRT771019	hsa-miR-4684-5p	PAR-CLIP	27292025
MIRT774337	hsa-miR-548c-3p	PAR-CLIP	27292025
MIRT775339	hsa-miR-585-3p	PAR-CLIP	27292025
MIRT778650	hsa-miR-6771-3p	PAR-CLIP	27292025
MIRT779933	hsa-miR-6827-3p	PAR-CLIP	27292025

Transcription factors

Transcription factor	Regulation	Reference
TWIST1	Repression	19051271
TWIST2	Repression	19051271

Gene ontology (GO)

GO ID	Ontology	Definition	Evidence	Reference
GO:0003924	Function	GTPase activity	IBA	21873635
GO:0005085	Function	Guanyl-nucleotide exchange factor activity	ISS
GO:0005515	Function	Protein binding	IPI	7791872, 8756332, 10608844, 11022048, 11359771, 12845325, 15569673, 16698549, 17916086
GO:0005525	Function	GTP binding	IBA	21873635
GO:0005737	Component	Cytoplasm	IDA	11359771, 23209302
GO:0005769	Component	Early endosome	ISS
GO:0005770	Component	Late endosome	ISS
GO:0005829	Component	Cytosol	TAS
GO:0005886	Component	Plasma membrane	IBA	21873635
GO:0005886	Component	Plasma membrane	TAS
GO:0007399	Process	Nervous system development	IEA
GO:0009743	Process	Response to carbohydrate	IEA
GO:0010976	Process	Positive regulation of neuron projection development	ISS
GO:0019003	Function	GDP binding	IBA	21873635
GO:0030033	Process	Microvillus assembly	IMP	22797597
GO:0030054	Component	Cell junction	ISS
GO:0031267	Function	Small GTPase binding	IEA
GO:0032045	Component	Guanyl-nucleotide exchange factor complex	IEA
GO:0032486	Process	Rap protein signal transduction	IBA	21873635
GO:0032486	Process	Rap protein signal transduction	IMP	21840392
GO:0032966	Process	Negative regulation of collagen biosynthetic process	IEA
GO:0035579	Component	Specific granule membrane	TAS
GO:0035690	Process	Cellular response to drug	IEA
GO:0038180	Process	Nerve growth factor signaling pathway	ISS
GO:0043005	Component	Neuron projection	IEA
GO:0043312	Process	Neutrophil degranulation	TAS
GO:0043547	Process	Positive regulation of GTPase activity	ISS
GO:0044877	Function	Protein-containing complex binding	IDA	23209302
GO:0045335	Component	Phagocytic vesicle	IEA
GO:0045860	Process	Positive regulation of protein kinase activity	ISS
GO:0046326	Process	Positive regulation of glucose import	IEA
GO:0048471	Component	Perinuclear region of cytoplasm	IDA	11359771
GO:0050796	Process	Regulation of insulin secretion	TAS
GO:0061028	Process	Establishment of endothelial barrier	IMP	21840392
GO:0070062	Component	Extracellular exosome	HDA	20458337
GO:0070374	Process	Positive regulation of ERK1 and ERK2 cascade	ISS
GO:0071320	Process	Cellular response to cAMP	IBA	21873635
GO:0071320	Process	Cellular response to cAMP	IDA	21840392
GO:0072659	Process	Protein localization to plasma membrane	IDA	17916086
GO:0097327	Process	Response to antineoplastic agent	IEA
GO:0097421	Process	Liver regeneration	IEA
GO:0098696	Process	Regulation of neurotransmitter receptor localization to postsynaptic specialization membrane	IDA	12202034
GO:0098696	Process	Regulation of neurotransmitter receptor localization to postsynaptic specialization membrane	IMP	12202034
GO:0098978	Component	Glutamatergic synapse	IDA	12202034
GO:0098978	Component	Glutamatergic synapse	IMP	12202034
GO:1901888	Process	Regulation of cell junction assembly	IMP	21840392
GO:1905451	Process	Positive regulation of Fc-gamma receptor signaling pathway involved in phagocytosis	IEA
GO:1990090	Process	Cellular response to nerve growth factor stimulus	ISS
GO:2000301	Process	Negative regulation of synaptic vesicle exocytosis	IBA	21873635
GO:2001214	Process	Positive regulation of vasculogenesis	ISS

Other IDs

• MIM: 179520
• HGNC: 9855
• e!Ensembl: ENSG00000116473

Protein

• UniProt ID: P62834
• Protein name: Ras-related protein Rap-1A (EC 3.6.5.2) (C21KG) (G-22K) (GTP-binding protein smg p21A) (Ras-related protein Krev-1)
• Protein function: Induces morphological reversion of a cell line transformed by a Ras oncogene. Counteracts the mitogenic function of Ras, at least partly because it can interact with Ras GAPs and RAF in a competitive manner. Together with ITGB1BP1, regulates KRIT1 localization to microtubules and membranes. Plays a role in nerve growth factor (NGF)-induced neurite outgrowth. Plays a role in the regulation of embryonic blood vessel formation. Involved in the establishment of basal endothelial barrier function. May be involved in the regulation of the vascular endothelial growth factor receptor KDR expression at endothelial cell-cell junctions.
• PDB: 1C1Y, 1GUA, 3KUC, 4KVG
• Family and domains:

  Pfam

  Accession	ID	Position in sequence	Description	Type
  PF00071	Ras	5 → 167	Ras family	Domain

• Sequence:

  MREYKLVVLGSGGVGKSALTVQFVQGIFVEKYDPTIEDSYRKQVEVDCQQCMLEILDTAG
  TEQFTAMRDLYMKNGQGFALVYSITAQSTFNDLQDLREQILRVKDTEDVPMILVGNKCDL
  EDERVVGKEQGQNLARQWCNCAFLESSAKSKINVNEIFYDLVRQINRKTPVEKKKPKKKS
  CLLL

• Sequence length: 184