PSME4 (proteasome activator subunit 4)

Gene

• Entrez ID: 23198
• Gene name: Proteasome activator subunit 4
• Gene symbol: PSME4
• Synonyms: PA200
• Chromosome: 2
• Chromosome location: 2p16.2

miRNA

miRTarBase ID	miRNA	Experiments	Reference
MIRT019934	hsa-miR-375	Microarray	20215506
MIRT020902	hsa-miR-155-5p	Proteomics	18668040
MIRT027763	hsa-miR-98-5p	Microarray	19088304
MIRT028604	hsa-miR-30a-5p	Proteomics	18668040
MIRT031835	hsa-miR-16-5p	Proteomics	18668040
MIRT035894	hsa-miR-1303	CLASH	23622248
MIRT051708	hsa-let-7e-5p	CLASH	23622248
MIRT439882	hsa-miR-218-5p	HITS-CLIP	23212916
MIRT716823	hsa-miR-7856-5p	HITS-CLIP	19536157
MIRT716824	hsa-miR-4477b	HITS-CLIP	19536157
MIRT716825	hsa-miR-142-3p	HITS-CLIP	19536157
MIRT716826	hsa-miR-4326	HITS-CLIP	19536157
MIRT716827	hsa-miR-6073	HITS-CLIP	19536157
MIRT716828	hsa-miR-20b-3p	HITS-CLIP	19536157
MIRT716829	hsa-miR-4255	HITS-CLIP	19536157
MIRT716830	hsa-miR-1183	HITS-CLIP	19536157
MIRT716831	hsa-miR-651-3p	HITS-CLIP	19536157

Gene ontology (GO)

GO ID	Ontology	Definition	Evidence	Reference
GO:0000165	Process	MAPK cascade	TAS
GO:0000209	Process	Protein polyubiquitination	TAS
GO:0002223	Process	Stimulatory C-type lectin receptor signaling pathway	TAS
GO:0002479	Process	Antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent	TAS
GO:0005515	Function	Protein binding	IPI	25416956
GO:0005634	Component	Nucleus	HDA	21630459
GO:0005634	Component	Nucleus	IBA	21873635
GO:0005634	Component	Nucleus	ISS
GO:0005654	Component	Nucleoplasm	IDA
GO:0005829	Component	Cytosol	IBA	21873635
GO:0005829	Component	Cytosol	ISS
GO:0005829	Component	Cytosol	TAS
GO:0006281	Process	DNA repair	ISS
GO:0006521	Process	Regulation of cellular amino acid metabolic process	TAS
GO:0006974	Process	Cellular response to DNA damage stimulus	ISS
GO:0007275	Process	Multicellular organism development	IEA
GO:0010499	Process	Proteasomal ubiquitin-independent protein catabolic process	IBA	21873635
GO:0010499	Process	Proteasomal ubiquitin-independent protein catabolic process	ISS
GO:0010952	Process	Positive regulation of peptidase activity	IEA
GO:0010972	Process	Negative regulation of G2/M transition of mitotic cell cycle	TAS
GO:0016504	Function	Peptidase activator activity	IBA	21873635
GO:0016504	Function	Peptidase activator activity	ISS
GO:0016579	Process	Protein deubiquitination	TAS
GO:0016607	Component	Nuclear speck	IEA
GO:0031145	Process	Anaphase-promoting complex-dependent catabolic process	TAS
GO:0031146	Process	SCF-dependent proteasomal ubiquitin-dependent protein catabolic process	TAS
GO:0033209	Process	Tumor necrosis factor-mediated signaling pathway	TAS
GO:0035093	Process	Spermatogenesis, exchange of chromosomal proteins	IBA	21873635
GO:0035093	Process	Spermatogenesis, exchange of chromosomal proteins	ISS
GO:0038061	Process	NIK/NF-kappaB signaling	TAS
GO:0038095	Process	Fc-epsilon receptor signaling pathway	TAS
GO:0043161	Process	Proteasome-mediated ubiquitin-dependent protein catabolic process	TAS
GO:0043488	Process	Regulation of mRNA stability	TAS
GO:0043687	Process	Post-translational protein modification	TAS
GO:0050852	Process	T cell receptor signaling pathway	TAS
GO:0055085	Process	Transmembrane transport	TAS
GO:0060071	Process	Wnt signaling pathway, planar cell polarity pathway	TAS
GO:0061418	Process	Regulation of transcription from RNA polymerase II promoter in response to hypoxia	TAS
GO:0070498	Process	Interleukin-1-mediated signaling pathway	TAS
GO:0070577	Function	Lysine-acetylated histone binding	IBA	21873635
GO:0070577	Function	Lysine-acetylated histone binding	ISS
GO:0070628	Function	Proteasome binding	IBA	21873635
GO:0090090	Process	Negative regulation of canonical Wnt signaling pathway	TAS
GO:0090263	Process	Positive regulation of canonical Wnt signaling pathway	TAS
GO:1901990	Process	Regulation of mitotic cell cycle phase transition	TAS
GO:1902036	Process	Regulation of hematopoietic stem cell differentiation	TAS
GO:1990111	Component	Spermatoproteasome complex	IBA	21873635
GO:1990111	Component	Spermatoproteasome complex	ISS

Other IDs

• MIM: 607705
• HGNC: 20635
• e!Ensembl: ENSG00000068878

Protein

• UniProt ID: Q14997
• Protein name: Proteasome activator complex subunit 4 (Proteasome activator PA200)
• Protein function: Associated component of the proteasome that specifically recognizes acetylated histones and promotes ATP- and ubiquitin-independent degradation of core histones during spermatogenesis and DNA damage response. Recognizes and binds acetylated histones via its bromodomain-like (BRDL) region and activates the proteasome by opening the gated channel for substrate entry. Binds to the core proteasome via its C-terminus, which occupies the same binding sites as the proteasomal ATPases, opening the closed structure of the proteasome via an active gating mechanism. Component of the spermatoproteasome, a form of the proteasome specifically found in testis: binds to acetylated histones and promotes degradation of histones, thereby participating actively to the exchange of histones during spermatogenesis. Also involved in DNA damage response in somatic cells, by promoting degradation of histones following DNA double-strand breaks.
• PDB: 6KWX, 6KWY, 6REY
• Family and domains:

  Pfam

  Accession	ID	Position in sequence	Description	Type
  PF16507	BLM10_mid	330 → 828	Proteasome-substrate-size regulator, mid region	Family
  PF11919	DUF3437	1756 → 1843	Domain of unknown function (DUF3437)	Family

• Sequence:

  MEPAERAGVGEPPEPGGRPEPGPRGFVPQKEIVYNKLLPYAERLDAESDLQLAQIKCNLG
  RAVQLQELWPGGLFWTRKLSTYIRLYGRKFSKEDHVLFIKLLYELVSIPKLEISMMQGFA
  RLLINLLKKKELLSRADLELPWRPLYDMVERILYSKTEHLGLNWFPNSVENILKTLVKSC
  RPYFPADATAEMLEEWRPLMCPFDVTMQKAITYFEIFLPTSLPPELHHKGFKLWFDELIG
  LWVSVQNLPQWEGQLVNLFARLATDNIGYIDWDPYVPKIFTRILRSLNLPVGSSQVLVPR
  FLTNAYDIGHAVIWITAMMGGPSKLVQKHLAGLFNSITSFYHPSNNGRWLNKLMKLLQRL
  PNSVVRRLHRERYKKPSWLTPVPDSHKLTDQDVTDFVQCIIQPVLLAMFSKTGSLEAAQA
  LQNLALMRPELVIPPVLERTYPALETLTEPHQLTATLSCVIGVARSLVSGGRWFPEGPTH
  MLPLLMRALPGVDPNDFSKCMITFQFIATFSTLVPLVDCSSVLQERNDLTEVERELCSAT
  AEFEDFVLQFMDRCFGLIESSTLEQTREETETEKMTHLESLVELGLSSTFSTILTQCSKE
  IFMVALQKVFNFSTSHIFETRVAGRMVADMCRAAVKCCPEESLKLFVPHCCSVITQLTMN
  DDVLNDEELDKELLWNLQLLSEITRVDGRKLLLYREQLVKILQRTLHLTCKQGYTLSCNL
  LHHLLRSTTLIYPTEYCSVPGGFDKPPSEYFPIKDWGKPGDLWNLGIQWHVPSSEEVSFA
  FYLLDSFLQPELVKLQHCGDGKLEMSRDDILQSLTIVHNCLIGSGNLLPPLKGEPVTNLV
  PSMVSLEETKLYTGLEYDLSRENHREVIATVIRKLLNHILDNSEDDTKSLFLIIKIIGDL
  LQFQGSHKHEFDSRWKSFNLVKKSMENRLHGKKQHIRALLIDRVMLQHELRTLTVEGCEY
  KKIHQDMIRDLLRLSTSSYSQVRNKAQQTFFAALGAYNFCCRDIIPLVLEFLRPDRQGVT
  QQQFKGALYCLLGNHSGVCLANLHDWDCIVQTWPAIVSSGLSQAMSLEKPSIVRLFDDLA
  EKIHRQYETIGLDFTIPKSCVEIAELLQQSKNPSINQILLSPEKIKEGIKRQQEKNADAL
  RNYENLVDTLLDGVEQRNLPWKFEHIGIGLLSLLLRDDRVLPLRAIRFFVENLNHDAIVV
  RKMAISAVAGILKQLKRTHKKLTINPCEISGCPKPTQIIAGDRPDNHWLHYDSKTIPRTK
  KEWESSCFVEKTHWGYYTWPKNMVVYAGVEEQPKLGRSREDMTEAEQIIFDHFSDPKFVE
  QLITFLSLEDRKGKDKFNPRRFCLFKGIFRNFDDAFLPVLKPHLEHLVADSHESTQRCVA
  EIIAGLIRGSKHWTFEKVEKLWELLCPLLRTALSNITVETYNDWGACIATSCESRDPRKL
  HWLFELLLESPLSGEGGSFVDACRLYVLQGGLAQQEWRVPELLHRLLKYLEPKLTQVYKN
  VRERIGSVLTYIFMIDVSLPNTTPTISPHVPEFTARILEKLKPLMDVDEEIQNHVMEENG
  IGEEDERTQGIKLLKTILKWLMASAGRSFSTAVTEQLQLLPLFFKIAPVENDNSYDELKR
  DAKLCLSLMSQGLLYPHQVPLVLQVLKQTARSSSWHARYTVLTYLQTMVFYNLFIFLNNE
  DAVKDIRWLVISLLEDEQLEVREMAATTLSGLLQCNFLTMDSPMQIHFEQLCKTKLPKKR
  KRDPGSVGDTIPSAELVKRHAGVLGLGACVLSSPYDVPTWMPQLLMNLSAHLNDPQPIEM
  TVKKTLSNFRRTHHDNWQEHKQQFTDDQLLVLTDLLVSPCYYA

• Sequence length: 1843