PRKACB (protein kinase cAMP-activated catalytic subunit beta)

Gene

• Entrez ID: 5567
• Gene name: Protein kinase cAMP-activated catalytic subunit beta
• Gene symbol: PRKACB
• Synonyms: CAFD2, PKA C-beta, PKACB
• Chromosome: 1
• Chromosome location: 1p31.1
• Summary: The protein encoded by this gene is a member of the serine/threonine protein kinase family. The encoded protein is a catalytic subunit of cAMP (cyclic AMP)-dependent protein kinase, which mediates signalling though cAMP. cAMP signaling is important to a number of processes, including cell proliferaton and differentiation. Multiple alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2014]

miRNA

miRTarBase ID	miRNA	Experiments	Reference
MIRT006806	hsa-miR-23b-3p	Luciferase reporter assay, qRT-PCR	22449550
MIRT006806	hsa-miR-23b-3p	Luciferase reporter assay, Immunocytochemistry, Western blot	24916040
MIRT027986	hsa-miR-93-5p	Sequencing	20371350
MIRT027986	hsa-miR-93-5p	PAR-CLIP	21572407
MIRT031110	hsa-miR-19b-3p	Sequencing	20371350
MIRT031110	hsa-miR-19b-3p	PAR-CLIP	21572407
MIRT080176	hsa-miR-17-5p	PAR-CLIP	21572407
MIRT080177	hsa-miR-19a-3p	PAR-CLIP	21572407
MIRT080178	hsa-miR-20a-5p	PAR-CLIP	21572407
MIRT080179	hsa-miR-106a-5p	PAR-CLIP	21572407
MIRT080180	hsa-miR-203a-3p	PAR-CLIP	21572407
MIRT080181	hsa-miR-106b-5p	PAR-CLIP	21572407
MIRT080182	hsa-miR-302a-3p	PAR-CLIP	21572407
MIRT080183	hsa-miR-302b-3p	PAR-CLIP	21572407
MIRT080184	hsa-miR-302c-3p	PAR-CLIP	21572407
MIRT080185	hsa-miR-302d-3p	PAR-CLIP	21572407
MIRT080186	hsa-miR-372-3p	PAR-CLIP	21572407
MIRT080187	hsa-miR-373-3p	PAR-CLIP	21572407
MIRT080188	hsa-miR-20b-5p	PAR-CLIP	21572407
MIRT080190	hsa-miR-520e	PAR-CLIP	21572407
MIRT080191	hsa-miR-520f-3p	PAR-CLIP	21572407
MIRT080193	hsa-miR-520a-3p	PAR-CLIP	21572407
MIRT080194	hsa-miR-526b-3p	PAR-CLIP	21572407
MIRT080196	hsa-miR-520b	PAR-CLIP	21572407
MIRT080197	hsa-miR-520c-3p	PAR-CLIP	21572407
MIRT080198	hsa-miR-519d-3p	PAR-CLIP	21572407
MIRT080199	hsa-miR-520d-3p	PAR-CLIP	21572407
MIRT080201	hsa-miR-513a-3p	PAR-CLIP	21572407
MIRT080203	hsa-miR-636	PAR-CLIP	21572407
MIRT080204	hsa-miR-548f-5p	PAR-CLIP	21572407
MIRT080205	hsa-miR-548g-5p	PAR-CLIP	21572407
MIRT080206	hsa-miR-302e	PAR-CLIP	21572407
MIRT080207	hsa-miR-513c-3p	PAR-CLIP	21572407
MIRT080209	hsa-miR-548x-5p	PAR-CLIP	21572407
MIRT080210	hsa-miR-3606-3p	PAR-CLIP	21572407
MIRT080211	hsa-miR-3622a-3p	PAR-CLIP	21572407
MIRT080212	hsa-miR-3622b-3p	PAR-CLIP	21572407
MIRT080213	hsa-miR-1273e	PAR-CLIP	21572407
MIRT080214	hsa-miR-3660	PAR-CLIP	21572407
MIRT080215	hsa-miR-548aj-5p	PAR-CLIP	21572407
MIRT080216	hsa-miR-4526	PAR-CLIP	21572407
MIRT080217	hsa-miR-548aw	PAR-CLIP	21572407
MIRT555213	hsa-miR-3692-3p	PAR-CLIP	21572407
MIRT555214	hsa-miR-7158-3p	PAR-CLIP	21572407
MIRT555215	hsa-miR-1468-3p	PAR-CLIP	21572407
MIRT555216	hsa-miR-2277-3p	PAR-CLIP	21572407
MIRT555217	hsa-miR-4539	PAR-CLIP	21572407
MIRT555218	hsa-miR-4724-5p	PAR-CLIP	21572407
MIRT555219	hsa-miR-3120-3p	PAR-CLIP	21572407

Gene ontology (GO)

GO ID	Ontology	Definition	Evidence	Reference
GO:0000287	Function	Magnesium ion binding	IDA	12420224
GO:0001843	Process	Neural tube closure	IEA
GO:0002223	Process	Stimulatory C-type lectin receptor signaling pathway	TAS
GO:0003091	Process	Renal water homeostasis	TAS
GO:0004679	Function	AMP-activated protein kinase activity	IEA
GO:0004691	Function	CAMP-dependent protein kinase activity	IDA	12420224
GO:0005515	Function	Protein binding	IPI	22939624, 23455922, 25416956, 32296183, 32814053
GO:0005524	Function	ATP binding	IDA	12420224
GO:0005634	Component	Nucleus	IBA	21873635
GO:0005654	Component	Nucleoplasm	TAS
GO:0005813	Component	Centrosome	IDA	21399614
GO:0005829	Component	Cytosol	TAS
GO:0005886	Component	Plasma membrane	IEA
GO:0005952	Component	CAMP-dependent protein kinase complex	TAS	12420224
GO:0006468	Process	Protein phosphorylation	IDA	12420224, 21880142
GO:0007165	Process	Signal transduction	TAS	2342480
GO:0007188	Process	Adenylate cyclase-modulating G protein-coupled receptor signaling pathway	TAS	12420224
GO:0007596	Process	Blood coagulation	TAS
GO:0010737	Process	Protein kinase A signaling	IBA	21873635
GO:0031625	Function	Ubiquitin protein ligase binding	IDA	21423175
GO:0034199	Process	Activation of protein kinase A activity	TAS
GO:0034380	Process	High-density lipoprotein particle assembly	TAS
GO:0048471	Component	Perinuclear region of cytoplasm	IEA
GO:0051447	Process	Negative regulation of meiotic cell cycle	IEA
GO:0070062	Component	Extracellular exosome	HDA	23533145
GO:0070613	Process	Regulation of protein processing	IEA
GO:0071377	Process	Cellular response to glucagon stimulus	TAS
GO:0097338	Process	Response to clozapine	IEA
GO:0097546	Component	Ciliary base	TAS
GO:1901621	Process	Negative regulation of smoothened signaling pathway involved in dorsal/ventral neural tube patterning	IEA

Other IDs

• MIM: 176892
• HGNC: 9381
• e!Ensembl: ENSG00000142875

Protein

• UniProt ID: P22694
• Protein name: cAMP-dependent protein kinase catalytic subunit beta (PKA C-beta) (EC 2.7.11.11)
• Protein function: Mediates cAMP-dependent signaling triggered by receptor binding to GPCRs. PKA activation regulates diverse cellular processes such as cell proliferation, the cell cycle, differentiation and regulation of microtubule dynamics, chromatin condensation and decondensation, nuclear envelope disassembly and reassembly, as well as regulation of intracellular transport mechanisms and ion flux. Regulates the abundance of compartmentalized pools of its regulatory subunits through phosphorylation of PJA2 which binds and ubiquitinates these subunits, leading to their subsequent proteolysis (PubMed:12420224, PubMed:21423175). Phosphorylates GPKOW which regulates its ability to bind RNA (PubMed:21880142).
• Family and domains:

  Pfam

  Accession	ID	Position in sequence	Description	Type
  PF00069	Pkinase	44 → 298	Protein kinase domain	Domain

• Sequence:

  MGNAATAKKGSEVESVKEFLAKAKEDFLKKWENPTQNNAGLEDFERKKTLGTGSFGRVML
  VKHKATEQYYAMKILDKQKVVKLKQIEHTLNEKRILQAVNFPFLVRLEYAFKDNSNLYMV
  MEYVPGGEMFSHLRRIGRFSEPHARFYAAQIVLTFEYLHSLDLIYRDLKPENLLIDHQGY
  IQVTDFGFAKRVKGRTWTLCGTPEYLAPEIILSKGYNKAVDWWALGVLIYEMAAGYPPFF
  ADQPIQIYEKIVSGKVRFPSHFSSDLKDLLRNLLQVDLTKRFGNLKNGVSDIKTHKWFAT
  TDWIAIYQRKVEAPFIPKFRGSGDTSNFDDYEEEDIRVSITEKCAKEFGEF

• Sequence length: 351