PPAT (phosphoribosyl pyrophosphate amidotransferase)

Gene

• Entrez ID: 5471
• Gene name: Phosphoribosyl pyrophosphate amidotransferase
• Gene symbol: PPAT
• Synonyms: ATASE, GPAT, PRAT
• Chromosome: 4
• Chromosome location: 4q12
• Summary: The protein encoded by this gene is a member of the purine/pyrimidine phosphoribosyltransferase family. It is a regulatory allosteric enzyme that catalyzes the first step of de novo purine nucleotide biosythetic pathway. This gene and PAICS/AIRC gene, a bifunctional enzyme catalyzing steps six and seven of this pathway, are located in close proximity on chromosome 4, and divergently transcribed from an intergenic region. [provided by RefSeq, Mar 2011]

miRNA

miRTarBase ID	miRNA	Experiments	Reference
MIRT005010	hsa-miR-125b-5p	Microarray	17891175
MIRT024474	hsa-miR-215-5p	Microarray	19074876
MIRT026845	hsa-miR-192-5p	Microarray	19074876
MIRT027826	hsa-miR-98-5p	Microarray	19088304
MIRT030142	hsa-miR-26b-5p	Microarray	19088304
MIRT039332	hsa-miR-425-5p	CLASH	23622248

Gene ontology (GO)

GO ID	Ontology	Definition	Evidence	Reference
GO:0000082	Process	G1/S transition of mitotic cell cycle	IEA
GO:0001822	Process	Kidney development	IEA
GO:0004044	Function	Amidophosphoribosyltransferase activity	IBA	21873635
GO:0005829	Component	Cytosol	TAS
GO:0006164	Process	Purine nucleotide biosynthetic process	IBA	21873635
GO:0006189	Process	'de novo' IMP biosynthetic process	IEA
GO:0006543	Process	Glutamine catabolic process	IEA
GO:0007595	Process	Lactation	IEA
GO:0009113	Process	Purine nucleobase biosynthetic process	IEA
GO:0009116	Process	Nucleoside metabolic process	IEA
GO:0009168	Process	Purine ribonucleoside monophosphate biosynthetic process	TAS
GO:0031100	Process	Animal organ regeneration	IEA
GO:0032869	Process	Cellular response to insulin stimulus	IEA
GO:0035690	Process	Cellular response to drug	IEA
GO:0042802	Function	Identical protein binding	IEA
GO:0046872	Function	Metal ion binding	IEA
GO:0051539	Function	4 iron, 4 sulfur cluster binding	IEA
GO:0060135	Process	Maternal process involved in female pregnancy	IEA

Other IDs

• MIM: 172450
• HGNC: 9238
• e!Ensembl: ENSG00000128059

Protein

• UniProt ID: Q06203
• Protein name: Amidophosphoribosyltransferase (ATase) (EC 2.4.2.14) (Glutamine phosphoribosylpyrophosphate amidotransferase) (GPAT)
• Family and domains:

  Pfam

  Accession	ID	Position in sequence	Description	Type
  PF13522	GATase_6	64 → 216		Domain

• Sequence:

  MELEELGIREECGVFGCIASGEWPTQLDVPHVITLGLVGLQHRGQESAGIVTSDGSSVPT
  FKSHKGMGLVNHVFTEDNLKKLYVSNLGIGHTRYATTGKCELENCQPFVVETLHGKIAVA
  HNGELVNAARLRKKLLRHGIGLSTSSDSEMITQLLAYTPPQEQDDTPDWVARIKNLMKEA
  PTAYSLLIMHRDVIYAVRDPYGNRPLCIGRLIPVSDINDKEKKTSETEGWVVSSESCSFL
  SIGARYYREVLPGEIVEISRHNVQTLDIISRSEGNPVAFCIFEYVYFARPDSMFEDQMVY
  TVRYRCGQQLAIEAPVDADLVSTVPESATPAALAYAGKCGLPYVEVLCKNRYVGRTFIQP
  NMRLRQLGVAKKFGVLSDNFKGKRIVLVDDSIVRGNTISPIIKLLKESGAKEVHIRVASP
  PIKYPCFMGINIPTKEELIANKPEFDHLAEYLGANSVVYLSVEGLVSSVQEGIKFKKQKE
  KKHDIMIQENGNGLECFEKSGHCTACLTGKYPVELEW

• Sequence length: 517