POLB (DNA polymerase beta)

Gene

• Entrez ID: 5423
• Gene name: DNA polymerase beta
• Gene symbol: POLB
• Synonyms: -
• Chromosome: 8
• Chromosome location: 8p11.21
• Summary: The protein encoded by this gene is a DNA polymerase involved in base excision and repair, also called gap-filling DNA synthesis. The encoded protein, acting as a monomer, is normally found in the cytoplasm, but it translocates to the nucleus upon DNA damage. Several transcript variants of this gene exist, but the full-length nature of only one has been described to date. [provided by RefSeq, Sep 2011]

miRNA

miRTarBase ID	miRNA	Experiments	Reference
MIRT035975	hsa-miR-1301-3p	CLASH	23622248
MIRT051150	hsa-miR-16-5p	CLASH	23622248

Transcription factors

Transcription factor	Regulation	Reference
ATF2	Activation	10518804

Gene ontology (GO)

GO ID	Ontology	Definition	Evidence	Reference
GO:0001701	Process	In utero embryonic development	IEA
GO:0003684	Function	Damaged DNA binding	IEA
GO:0003887	Function	DNA-directed DNA polymerase activity	IBA	21873635
GO:0003887	Function	DNA-directed DNA polymerase activity	IDA	9207062, 16600869
GO:0003887	Function	DNA-directed DNA polymerase activity	TAS
GO:0003906	Function	DNA-(apurinic or apyrimidinic site) endonuclease activity	TAS
GO:0005515	Function	Protein binding	IPI	9207062, 15520167, 25260657, 25416956, 25910212, 26496610, 28514442, 31515488, 32814053
GO:0005634	Component	Nucleus	IBA	21873635
GO:0005634	Component	Nucleus	IDA	19713937, 21362556
GO:0005654	Component	Nucleoplasm	TAS
GO:0005737	Component	Cytoplasm	IDA	15725623, 19713937, 21362556
GO:0005874	Component	Microtubule	IDA	15725623
GO:0005876	Component	Spindle microtubule	IDA	15725623
GO:0006261	Process	DNA-dependent DNA replication	TAS	8168825
GO:0006281	Process	DNA repair	TAS	8168825
GO:0006284	Process	Base-excision repair	IBA	21873635
GO:0006284	Process	Base-excision repair	IDA	16600869
GO:0006284	Process	Base-excision repair	IMP	21362556
GO:0006286	Process	Base-excision repair, base-free sugar-phosphate removal	TAS
GO:0006287	Process	Base-excision repair, gap-filling	IEA
GO:0006288	Process	Base-excision repair, DNA ligation	TAS
GO:0006290	Process	Pyrimidine dimer repair	IEA
GO:0006297	Process	Nucleotide-excision repair, DNA gap filling	IMP	20227374
GO:0006303	Process	Double-strand break repair via nonhomologous end joining	IBA	21873635
GO:0006954	Process	Inflammatory response	IEA
GO:0006974	Process	Cellular response to DNA damage stimulus	IMP	21362556
GO:0007435	Process	Salivary gland morphogenesis	IEA
GO:0007568	Process	Aging	IEA
GO:0008017	Function	Microtubule binding	IDA	15725623
GO:0008630	Process	Intrinsic apoptotic signaling pathway in response to DNA damage	IEA
GO:0010332	Process	Response to gamma radiation	IEA
GO:0016446	Process	Somatic hypermutation of immunoglobulin genes	IEA
GO:0016579	Process	Protein deubiquitination	TAS
GO:0016829	Function	Lyase activity	IDA	16600869
GO:0019899	Function	Enzyme binding	IPI	16600869, 19713937, 21362556
GO:0032991	Component	Protein-containing complex	IDA	25378300
GO:0045471	Process	Response to ethanol	IEA
GO:0046872	Function	Metal ion binding	IEA
GO:0048535	Process	Lymph node development	IEA
GO:0048536	Process	Spleen development	IEA
GO:0048872	Process	Homeostasis of number of cells	IEA
GO:0051402	Process	Neuron apoptotic process	IEA
GO:0055093	Process	Response to hyperoxia	IEA
GO:0071707	Process	Immunoglobulin heavy chain V-D-J recombination	IEA
GO:0071897	Process	DNA biosynthetic process	IEA

Other IDs

• MIM: 174760
• HGNC: 9174
• e!Ensembl: ENSG00000070501

Protein

• UniProt ID: P06746
• Protein name: DNA polymerase beta (EC 2.7.7.7) (EC 4.2.99.-)
• Protein function: Repair polymerase that plays a key role in base-excision repair. Has 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity that removes the 5' sugar phosphate and also acts as a DNA polymerase that adds one nucleotide to the 3' end of the arising single-nucleotide gap. Conducts 'gap-filling' DNA synthesis in a stepwise distributive fashion rather than in a processive fashion as for other DNA polymerases.
• PDB: 1BPX, 1BPY, 1BPZ, 1MQ2, 1MQ3, 1TV9, 1TVA, 1ZJM, 1ZJN, 1ZQA, 1ZQB, 1ZQC, 1ZQD, 1ZQE, 1ZQF, 1ZQG, 1ZQH, 1ZQI, 1ZQJ, 1ZQK, 1ZQL, 1ZQM, 1ZQN, 1ZQO, 1ZQP, 1ZQQ, 1ZQR, 1ZQS, 1ZQT, 2FMP, 2FMQ, 2FMS, 2I9G, 2ISO, 2ISP, 2P66, 2PXI, 3C2K, 3C2L, 3C2M, 3GDX, 3ISB, 3ISC, 3ISD, 3JPN, 3JPO, 3JPP, 3JPQ, 3JPR, 3JPS, 3JPT
• Family and domains:

  Pfam

  Accession	ID	Position in sequence	Description	Type
  PF14716	HHH_8	12 → 78	Helix-hairpin-helix domain	Domain
  PF10391	DNA_pol_lambd_f	98 → 147	Fingers domain of DNA polymerase lambda	Domain
  PF14792	DNA_pol_B_palm	149 → 262	DNA polymerase beta palm	Domain
  PF14791	DNA_pol_B_thumb	268 → 334	DNA polymerase beta thumb	Family

• Sequence:

  MSKRKAPQETLNGGITDMLTELANFEKNVSQAIHKYNAYRKAASVIAKYPHKIKSGAEAK
  KLPGVGTKIAEKIDEFLATGKLRKLEKIRQDDTSSSINFLTRVSGIGPSAARKFVDEGIK
  TLEDLRKNEDKLNHHQRIGLKYFGDFEKRIPREEMLQMQDIVLNEVKKVDSEYIATVCGS
  FRRGAESSGDMDVLLTHPSFTSESTKQPKLLHQVVEQLQKVHFITDTLSKGETKFMGVCQ
  LPSKNDEKEYPHRRIDIRLIPKDQYYCGVLYFTGSDIFNKNMRAHALEKGFTINEYTIRP
  LGVTGVAGEPLPVDSEKDIFDYIQWKYREPKDRSE

• Sequence length: 335