PIK3R3 (phosphoinositide-3-kinase regulatory subunit 3)

Gene

• Entrez ID: 8503
• Gene name: Phosphoinositide-3-kinase regulatory subunit 3
• Gene symbol: PIK3R3
• Synonyms: p55, p55-GAMMA, p55PIK
• Chromosome: 1
• Chromosome location: 1p34.1
• Summary: Phosphatidylinositol 3-kinase (PI3K) phosphorylates phosphatidylinositol and similar compounds, which then serve as second messengers in growth signaling pathways. PI3K is composed of a catalytic and a regulatory subunit. The protein encoded by this gene represents a regulatory subunit of PI3K. The encoded protein contains two SH2 domains through which it binds activated protein tyrosine kinases to regulate their activity. [provided by RefSeq, Jun 2016]

miRNA

miRTarBase ID	miRNA	Experiments	Reference
MIRT040803	hsa-miR-18a-3p	CLASH	23622248
MIRT042712	hsa-miR-346	CLASH	23622248
MIRT043293	hsa-miR-331-3p	CLASH	23622248
MIRT053541	hsa-miR-7-5p	ELISA, Luciferase reporter assay, qRT-PCR, Western blot	23135998
MIRT726938	hsa-miR-19b-3p	HITS-CLIP	22473208
MIRT726939	hsa-miR-19a-3p	HITS-CLIP	22473208
MIRT732434	hsa-miR-148b-3p	Western blot, Luciferase reporter assay,	24632606
MIRT734057	hsa-miR-193a-5p	Luciferase reporter assay, qRT-PCR, Western blot	24469061
MIRT734832	hsa-miR-132-3p	Luciferase reporter assay, qRT-PCR, Western blot	26252738
MIRT735447	hsa-miR-152-3p	Luciferase reporter assay, qRT-PCR, Western blot	26820128

Transcription factors

Transcription factor	Regulation	Reference
MZF1	Unknown	23509792

Gene ontology (GO)

GO ID	Ontology	Definition	Evidence	Reference
GO:0001784	Function	Phosphotyrosine residue binding	IPI	20624904
GO:0001934	Process	Positive regulation of protein phosphorylation	IMP	28341552
GO:0002042	Process	Cell migration involved in sprouting angiogenesis	IGI	28341552
GO:0005515	Function	Protein binding	IPI	16273093, 17360667, 17500595, 19380743, 20598684, 21706016, 22402981, 24189400, 24658140, 24728074, 25241761, 25814554, 26496610, 28514442, 31515488, 31980649, 32296183, 32606738, 32814053
GO:0005829	Component	Cytosol	TAS
GO:0005942	Component	Phosphatidylinositol 3-kinase complex	IBA	21873635
GO:0006661	Process	Phosphatidylinositol biosynthetic process	TAS
GO:0008286	Process	Insulin receptor signaling pathway	IBA	21873635
GO:0010628	Process	Positive regulation of gene expression	IMP	28341552
GO:0016303	Function	1-phosphatidylinositol-3-kinase activity	TAS	9524259
GO:0030335	Process	Positive regulation of cell migration	NAS	28260020
GO:0036092	Process	Phosphatidylinositol-3-phosphate biosynthetic process	IEA
GO:0043491	Process	Protein kinase B signaling	IMP	28341552
GO:0043551	Process	Regulation of phosphatidylinositol 3-kinase activity	IBA	21873635
GO:0046854	Process	Phosphatidylinositol phosphorylation	IBA	21873635
GO:0046935	Function	1-phosphatidylinositol-3-kinase regulator activity	IBA	21873635

Other IDs

• MIM: 606076
• HGNC: 8981
• e!Ensembl: ENSG00000117461

Protein

• UniProt ID: Q92569
• Protein name: Phosphatidylinositol 3-kinase regulatory subunit gamma (PI3-kinase regulatory subunit gamma) (PI3K regulatory subunit gamma) (PtdIns-3-kinase regulatory subunit gamma) (Phosphatidylinositol 3-kinase 55 kDa regulatory subunit gamma) (PI3-kinase subunit p55-gamma) (PtdIns-3-kinase regulatory subunit p55-gamma) (p55PIK)
• Protein function: Binds to activated (phosphorylated) protein-tyrosine kinases through its SH2 domain and regulates their kinase activity. During insulin stimulation, it also binds to IRS-1.
• Family and domains:

  Pfam

  Accession	ID	Position in sequence	Description	Type
  PF00017	SH2	65 → 140	SH2 domain	Domain
  PF16454	PI3K_P85_iSH2	163 → 331	Phosphatidylinositol 3-kinase regulatory subunit P85 inter-SH2 domain	Domain
  PF00017	SH2	358 → 432	SH2 domain	Domain

• Sequence:

  MYNTVWSMDRDDADWREVMMPYSTELIFYIEMDPPALPPKPPKPMTSAVPNGMKDSSVSL
  QDAEWYWGDISREEVNDKLRDMPDGTFLVRDASTKMQGDYTLTLRKGGNNKLIKIYHRDG
  KYGFSDPLTFNSVVELINHYHHESLAQYNPKLDVKLMYPVSRYQQDQLVKEDNIDAVGKK
  LQEYHSQYQEKSKEYDRLYEEYTRTSQEIQMKRTAIEAFNETIKIFEEQCHTQEQHSKEY
  IERFRREGNEKEIERIMMNYDKLKSRLGEIHDSKMRLEQDLKNQALDNREIDKKMNSIKP
  DLIQLRKIRDQHLVWLNHKGVRQKRLNVWLGIKNEDADENYFINEEDENLPHYDEKTWFV
  EDINRVQAEDLLYGKPDGAFLIRESSKKGCYACSVVADGEVKHCVIYSTARGYGFAEPYN
  LYSSLKELVLHYQQTSLVQHNDSLNVRLAYPVHAQMPSLCR

• Sequence length: 461