PIK3CG (phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit gamma)

Gene

• Entrez ID: 5294
• Gene name: Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit gamma
• Gene symbol: PIK3CG
• Synonyms: PI3CG, PI3K, PI3Kgamma, PIK3, p110gamma, p120-PI3K
• Chromosome: 7
• Chromosome location: 7q22.3
• Summary: Phosphoinositide 3-kinases (PI3Ks) phosphorylate inositol lipids and are involved in the immune response. The protein encoded by this gene is a class I catalytic subunit of PI3K. Like other class I catalytic subunits (p110-alpha p110-beta, and p110-delta), the encoded protein binds a p85 regulatory subunit to form PI3K. This gene is located in a commonly deleted segment of chromosome 7 previously identified in myeloid leukemias. Several transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jun 2015]

miRNA

miRTarBase ID	miRNA	Experiments	Reference
MIRT006815	hsa-miR-29b-3p	Microarray, qRT-PCR, Western blot	22297492
MIRT006831	hsa-miR-126-3p	Immunocytochemistry, Luciferase reporter assay, qRT-PCR, Western blot	22900072
MIRT006831	hsa-miR-126-3p	Microarray, qRT-PCR, Western blot, SILAC (Stable Isotope Labeling of Amino acids in Culture)	23142521
MIRT006831	hsa-miR-126-3p	Luciferase reporter assay	27729613
MIRT006831	hsa-miR-126-3p	qRT-PCR, Western blot	26659078
MIRT019349	hsa-miR-148b-3p	Microarray	17612493
MIRT029342	hsa-miR-26b-5p	Microarray	19088304
MIRT437359	hsa-miR-27a-3p	Luciferase reporter assay, qRT-PCR	24086481
MIRT438277	hsa-miR-7-5p	Luciferase reporter assay, Western blot	24603851
MIRT438408	hsa-miR-10a-5p	Luciferase reporter assay, qRT-PCR, Western blot	24522205
MIRT439973	hsa-miR-142-3p	HITS-CLIP	22473208
MIRT516704	hsa-miR-1277-5p	PAR-CLIP	23446348
MIRT516704	hsa-miR-1277-5p	PAR-CLIP, HITS-CLIP	21572407
MIRT516704	hsa-miR-1277-5p	HITS-CLIP	23313552
MIRT516705	hsa-miR-190a-3p	PAR-CLIP	23446348
MIRT516705	hsa-miR-190a-3p	PAR-CLIP, HITS-CLIP	21572407
MIRT516705	hsa-miR-190a-3p	HITS-CLIP	23313552
MIRT516706	hsa-miR-4789-5p	PAR-CLIP	23446348
MIRT516706	hsa-miR-4789-5p	PAR-CLIP, HITS-CLIP	21572407
MIRT516707	hsa-miR-3941	PAR-CLIP	23446348
MIRT516707	hsa-miR-3941	PAR-CLIP, HITS-CLIP	21572407
MIRT516708	hsa-miR-3689f	PAR-CLIP	23446348
MIRT516708	hsa-miR-3689f	HITS-CLIP	23313552
MIRT516709	hsa-miR-3689e	PAR-CLIP	23446348
MIRT516709	hsa-miR-3689e	HITS-CLIP	23313552
MIRT516710	hsa-miR-3689b-5p	PAR-CLIP	23446348
MIRT516710	hsa-miR-3689b-5p	HITS-CLIP	23313552
MIRT516711	hsa-miR-3689a-5p	PAR-CLIP	23446348
MIRT516711	hsa-miR-3689a-5p	HITS-CLIP	23313552
MIRT516712	hsa-miR-4679	PAR-CLIP	23446348
MIRT516712	hsa-miR-4679	HITS-CLIP	23313552
MIRT516713	hsa-miR-5011-5p	PAR-CLIP	23446348
MIRT516713	hsa-miR-5011-5p	PAR-CLIP, HITS-CLIP	21572407
MIRT516713	hsa-miR-5011-5p	HITS-CLIP	23313552
MIRT516714	hsa-miR-4789-3p	PAR-CLIP	23446348
MIRT516714	hsa-miR-4789-3p	PAR-CLIP, HITS-CLIP	21572407
MIRT516714	hsa-miR-4789-3p	HITS-CLIP	23313552
MIRT516715	hsa-miR-603	PAR-CLIP	23446348
MIRT516715	hsa-miR-603	PAR-CLIP, HITS-CLIP	21572407
MIRT516716	hsa-miR-362-3p	PAR-CLIP	23446348
MIRT516716	hsa-miR-362-3p	PAR-CLIP, HITS-CLIP	21572407
MIRT516717	hsa-miR-329-3p	PAR-CLIP	23446348
MIRT516717	hsa-miR-329-3p	PAR-CLIP, HITS-CLIP	21572407
MIRT516718	hsa-miR-8485	PAR-CLIP	23446348
MIRT516718	hsa-miR-8485	PAR-CLIP, HITS-CLIP	21572407
MIRT516718	hsa-miR-8485	HITS-CLIP	23313552
MIRT542012	hsa-miR-410-3p	PAR-CLIP	21572407
MIRT542012	hsa-miR-410-3p	HITS-CLIP	23313552
MIRT542013	hsa-miR-5580-3p	PAR-CLIP, HITS-CLIP	21572407
MIRT542013	hsa-miR-5580-3p	HITS-CLIP	23313552
MIRT693446	hsa-miR-511-3p	HITS-CLIP	23313552
MIRT693447	hsa-miR-3163	HITS-CLIP	23313552
MIRT693448	hsa-miR-548g-3p	HITS-CLIP	23313552
MIRT693449	hsa-miR-5582-3p	HITS-CLIP	23313552
MIRT693450	hsa-miR-548f-3p	HITS-CLIP	23313552
MIRT693451	hsa-miR-548e-3p	HITS-CLIP	23313552
MIRT693452	hsa-miR-548az-3p	HITS-CLIP	23313552
MIRT693453	hsa-miR-548ar-3p	HITS-CLIP	23313552
MIRT693454	hsa-miR-548a-3p	HITS-CLIP	23313552
MIRT732466	hsa-miR-127-3p	ELISA, qRT-PCR	25347932
MIRT733140	hsa-miR-196b-5p	Flow, qRT-PCR, Western blot	26998071
MIRT733151	hsa-miR-34a-5p	Flow, qRT-PCR, Western blot	27073535
MIRT734301	hsa-miR-125a-5p	qRT-PCR, Western blot	26622553
MIRT734392	hsa-miR-26a-5p	Immunohistochemistry, qRT-PCR, Western blot	26021873

Gene ontology (GO)

GO ID	Ontology	Definition	Evidence	Reference
GO:0001525	Process	Angiogenesis	IEA
GO:0001816	Process	Cytokine production	TAS	19075988
GO:0002250	Process	Adaptive immune response	TAS	17290298
GO:0002407	Process	Dendritic cell chemotaxis	TAS	18278175
GO:0002675	Process	Positive regulation of acute inflammatory response	IEA
GO:0002679	Process	Respiratory burst involved in defense response	TAS	19075988
GO:0003376	Process	Sphingosine-1-phosphate receptor signaling pathway	IMP	18558630
GO:0004672	Function	Protein kinase activity	TAS	19376709
GO:0005515	Function	Protein binding	IPI	12507995, 21393242, 23824069, 25556234, 32814053
GO:0005524	Function	ATP binding	IEA
GO:0005737	Component	Cytoplasm	IBA	21873635
GO:0005737	Component	Cytoplasm	IDA	12507995
GO:0005829	Component	Cytosol	IDA
GO:0005829	Component	Cytosol	TAS
GO:0005886	Component	Plasma membrane	IBA	21873635
GO:0005886	Component	Plasma membrane	IDA
GO:0005942	Component	Phosphatidylinositol 3-kinase complex	IBA	21873635
GO:0005943	Component	Phosphatidylinositol 3-kinase complex, class IA	IBA	21873635
GO:0005944	Component	Phosphatidylinositol 3-kinase complex, class IB	IBA	21873635
GO:0005944	Component	Phosphatidylinositol 3-kinase complex, class IB	IDA	12507995
GO:0006468	Process	Protein phosphorylation	IEA
GO:0006661	Process	Phosphatidylinositol biosynthetic process	TAS
GO:0006897	Process	Endocytosis	IEA
GO:0006954	Process	Inflammatory response	TAS	17290298
GO:0007186	Process	G protein-coupled receptor signaling pathway	IDA	12507995
GO:0007204	Process	Positive regulation of cytosolic calcium ion concentration	IEA
GO:0010595	Process	Positive regulation of endothelial cell migration	IMP	18558630
GO:0010818	Process	T cell chemotaxis	TAS	18278175
GO:0010897	Process	Negative regulation of triglyceride catabolic process	IEA
GO:0014065	Process	Phosphatidylinositol 3-kinase signaling	IBA	21873635
GO:0014065	Process	Phosphatidylinositol 3-kinase signaling	IDA	12507995
GO:0014065	Process	Phosphatidylinositol 3-kinase signaling	IMP	18558630
GO:0014065	Process	Phosphatidylinositol 3-kinase signaling	TAS	17290298
GO:0014068	Process	Positive regulation of phosphatidylinositol 3-kinase signaling	TAS
GO:0016020	Component	Membrane	HDA	19946888
GO:0016020	Component	Membrane	IBA	21873635
GO:0016020	Component	Membrane	IDA	12507995
GO:0016301	Function	Kinase activity	IDA	25327288
GO:0016303	Function	1-phosphatidylinositol-3-kinase activity	IBA	21873635
GO:0016303	Function	1-phosphatidylinositol-3-kinase activity	IDA	11277933, 16123124
GO:0016310	Process	Phosphorylation	IDA	25327288
GO:0016477	Process	Cell migration	IBA	21873635
GO:0030168	Process	Platelet activation	TAS
GO:0030593	Process	Neutrophil chemotaxis	TAS	17290298
GO:0032252	Process	Secretory granule localization	IEA
GO:0033628	Process	Regulation of cell adhesion mediated by integrin	TAS	18278175
GO:0035004	Function	Phosphatidylinositol 3-kinase activity	TAS	17290298
GO:0035005	Function	1-phosphatidylinositol-4-phosphate 3-kinase activity	IBA	21873635
GO:0035005	Function	1-phosphatidylinositol-4-phosphate 3-kinase activity	IDA	16123124
GO:0035022	Process	Positive regulation of Rac protein signal transduction	IMP	18558630
GO:0035747	Process	Natural killer cell chemotaxis	TAS	18278175
GO:0036092	Process	Phosphatidylinositol-3-phosphate biosynthetic process	IBA	21873635
GO:0042098	Process	T cell proliferation	TAS	19075988
GO:0042110	Process	T cell activation	TAS	17290298
GO:0042802	Function	Identical protein binding	IPI	20562859, 23824069
GO:0043303	Process	Mast cell degranulation	TAS	17290298
GO:0043406	Process	Positive regulation of MAP kinase activity	IDA	12507995
GO:0045087	Process	Innate immune response	TAS	17290298
GO:0046854	Process	Phosphatidylinositol phosphorylation	IBA	21873635
GO:0046875	Function	Ephrin receptor binding	IPI	11416136
GO:0046934	Function	Phosphatidylinositol-4,5-bisphosphate 3-kinase activity	IDA	15135396, 16123124
GO:0048015	Process	Phosphatidylinositol-mediated signaling	IBA	21873635
GO:0051897	Process	Positive regulation of protein kinase B signaling	IDA	12507995
GO:0052742	Function	Phosphatidylinositol kinase activity	IBA	21873635
GO:0052812	Function	Phosphatidylinositol-3,4-bisphosphate 5-kinase activity	IEA
GO:0055118	Process	Negative regulation of cardiac muscle contraction	TAS	19147653
GO:0070527	Process	Platelet aggregation	TAS	21035500
GO:0071320	Process	Cellular response to cAMP	IEA
GO:0072672	Process	Neutrophil extravasation	TAS	18278175
GO:0097284	Process	Hepatocyte apoptotic process	IEA
GO:0106310	Function	Protein serine kinase activity	IEA
GO:0106311	Function	Protein threonine kinase activity	IEA
GO:1903169	Process	Regulation of calcium ion transmembrane transport	IEA
GO:2000270	Process	Negative regulation of fibroblast apoptotic process	IEA

Other IDs

• MIM: 601232
• HGNC: 8978
• e!Ensembl: ENSG00000105851

Protein

• UniProt ID: P48736
• Protein name: Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit gamma isoform (PI3-kinase subunit gamma) (PI3K-gamma) (PI3Kgamma) (PtdIns-3-kinase subunit gamma) (EC 2.7.1.137) (EC 2.7.1.153) (EC 2.7.1.154) (Phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit gamma) (PtdIns-3-kinase subunit p110-gamma) (p110gamma) (Phosphoinositide-3-kinase catalytic gamma polypeptide) (Serine/threonine protein kinase PIK3CG) (EC 2.7.11.1) (p120-PI3K)
• Protein function: Phosphoinositide-3-kinase (PI3K) that phosphorylates PtdIns(4,5)P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Links G-protein coupled receptor activation to PIP3 production. Involved in immune, inflammatory and allergic responses. Modulates leukocyte chemotaxis to inflammatory sites and in response to chemoattractant agents. May control leukocyte polarization and migration by regulating the spatial accumulation of PIP3 and by regulating the organization of F-actin formation and integrin-based adhesion at the leading edge. Controls motility of dendritic cells. Together with PIK3CD is involved in natural killer (NK) cell development and migration towards the sites of inflammation. Participates in T-lymphocyte migration. Regulates T-lymphocyte proliferation and cytokine production. Together with PIK3CD participates in T-lymphocyte development. Required for B-lymphocyte development and signaling. Together with PIK3CD participates in neutrophil respiratory burst. Together with PIK3CD is involved in neutrophil chemotaxis and extravasation. Together with PIK3CB promotes platelet aggregation and thrombosis. Regulates alpha-IIb/beta-3 integrins (ITGA2B/ ITGB3) adhesive function in platelets downstream of P2Y12 through a lipid kinase activity-independent mechanism. May have also a lipid kinase activity-dependent function in platelet aggregation. Involved in endothelial progenitor cell migration. Negative regulator of cardiac contractility. Modulates cardiac contractility by anchoring protein kinase A (PKA) and PDE3B activation, reducing cAMP levels. Regulates cardiac contractility also by promoting beta-adrenergic receptor internalization by binding to GRK2 and by non-muscle tropomyosin phosphorylation. Also has serine/threonine protein kinase activity: both lipid and protein kinase activities are required for beta-adrenergic receptor endocytosis. May also have a scaffolding role in modulating cardiac contractility. Contributes to cardiac hypertrophy under pathological stress. Through simultaneous binding of PDE3B to RAPGEF3 and PIK3R6 is assembled in a signaling complex in which the PI3K gamma complex is activated by RAPGEF3 and which is involved in angiogenesis.
• PDB: 1E8Y, 1E8Z, 1HE8, 2A4Z, 2A5U, 2CHW, 2CHX, 2CHZ, 2V4L, 3APC, 3APD, 3APF, 3CSF, 3CST, 3DBS, 3DPD, 3ENE, 3IBE, 3L08, 3L13, 3L16, 3L17, 3L54, 3LJ3, 3MJW, 3ML8, 3ML9, 3NZS, 3NZU, 3OAW, 3P2B, 3PRE, 3PRZ, 3PS6, 3QAQ, 3QAR, 3QJZ, 3QK0, 3R7Q, 3R7R, 3S2A, 3SD5, 3T8M, 3TJP, 3TL5, 3ZVV, 3ZW3, 4ANU, 4ANV, 4ANW, 4ANX
• Family and domains:

  Pfam

  Accession	ID	Position in sequence	Description	Type
  PF00794	PI3K_rbd	203 → 312	PI3-kinase family, ras-binding domain	Domain
  PF00792	PI3K_C2	377 → 519	Phosphoinositide 3-kinase C2	Domain
  PF00613	PI3Ka	543 → 733	Phosphoinositide 3-kinase family, accessory domain (PIK domain)	Family
  PF00454	PI3_PI4_kinase	827 → 1044	Phosphatidylinositol 3- and 4-kinase	Family

• Sequence:

  MELENYKQPVVLREDNCRRRRRMKPRSAAASLSSMELIPIEFVLPTSQRKCKSPETALLH
  VAGHGNVEQMKAQVWLRALETSVAADFYHRLGPHHFLLLYQKKGQWYEIYDKYQVVQTLD
  CLRYWKATHRSPGQIHLVQRHPPSEESQAFQRQLTALIGYDVTDVSNVHDDELEFTRRGL
  VTPRMAEVASRDPKLYAMHPWVTSKPLPEYLWKKIANNCIFIVIHRSTTSQTIKVSPDDT
  PGAILQSFFTKMAKKKSLMDIPESQSEQDFVLRVCGRDEYLVGETPIKNFQWVRHCLKNG
  EEIHVVLDTPPDPALDEVRKEEWPLVDDCTGVTGYHEQLTIHGKDHESVFTVSLWDCDRK
  FRVKIRGIDIPVLPRNTDLTVFVEANIQHGQQVLCQRRTSPKPFTEEVLWNVWLEFSIKI
  KDLPKGALLNLQIYCGKAPALSSKASAESPSSESKGKVQLLYYVNLLLIDHRFLLRRGEY
  VLHMWQISGKGEDQGSFNADKLTSATNPDKENSMSISILLDNYCHPIALPKHQPTPDPEG
  DRVRAEMPNQLRKQLEAIIATDPLNPLTAEDKELLWHFRYESLKHPKAYPKLFSSVKWGQ
  QEIVAKTYQLLARREVWDQSALDVGLTMQLLDCNFSDENVRAIAVQKLESLEDDDVLHYL
  LQLVQAVKFEPYHDSALARFLLKRGLRNKRIGHFLFWFLRSEIAQSRHYQQRFAVILEAY
  LRGCGTAMLHDFTQQVQVIEMLQKVTLDIKSLSAEKYDVSSQVISQLKQKLENLQNSQLP
  ESFRVPYDPGLKAGALAIEKCKVMASKKKPLWLEFKCADPTALSNETIGIIFKHGDDLRQ
  DMLILQILRIMESIWETESLDLCLLPYGCISTGDKIGMIEIVKDATTIAKIQQSTVGNTG
  AFKDEVLNHWLKEKSPTEEKFQAAVERFVYSCAGYCVATFVLGIGDRHNDNIMITETGNL
  FHIDFGHILGNYKSFLGINKERVPFVLTPDFLFVMGTSGKKTSPHFQKFQDICVKAYLAL
  RHHTNLLIILFSMMLMTGMPQLTSKEDIEYIRDALTVGKNEEDAKKYFLDQIEVCRDKGW
  TVQFNWFLHLVLGIKQGEKHSA

• Sequence length: 1102