PCK2 (phosphoenolpyruvate carboxykinase 2, mitochondrial)

Gene

• Entrez ID: 5106
• Gene name: Phosphoenolpyruvate carboxykinase 2, mitochondrial
• Gene symbol: PCK2
• Synonyms: PEPCK, PEPCK-M, PEPCK2
• Chromosome: 14
• Chromosome location: 14q11.2-q12
• Summary: This gene encodes a mitochondrial enzyme that catalyzes the conversion of oxaloacetate to phosphoenolpyruvate in the presence of guanosine triphosphate (GTP). A cytosolic form of this protein is encoded by a different gene and is the key enzyme of gluconeogenesis in the liver. Alternatively spliced transcript variants have been described. [provided by RefSeq, Apr 2014]

miRNA

miRTarBase ID	miRNA	Experiments	Reference
MIRT030573	hsa-miR-24-3p	Microarray	19748357
MIRT031958	hsa-miR-16-5p	Proteomics	18668040

Transcription factors

Transcription factor	Regulation	Reference
ATF2	Unknown	11042264
CEBPA	Unknown	11042264
CEBPB	Activation	8093246
CREBBP	Unknown	10085123;11997389
FOS	Unknown	1325459
HDAC3	Unknown	17456789
JUN	Unknown	1325459
NCOR2	Unknown	17456789
NFIA	Unknown	10085123
NR0B2	Repression	17094771
NR0B2	Unknown	15358835
PPARGC1A	Activation	16891307
RELA	Repression	20137375
SRCAP	Unknown	11522779

Gene ontology (GO)

GO ID	Ontology	Definition	Evidence	Reference
GO:0004611	Function	Phosphoenolpyruvate carboxykinase activity	TAS	8645161
GO:0004613	Function	Phosphoenolpyruvate carboxykinase (GTP) activity	IBA	21873635
GO:0004613	Function	Phosphoenolpyruvate carboxykinase (GTP) activity	TAS
GO:0005515	Function	Protein binding	IPI	21988832
GO:0005525	Function	GTP binding	IEA
GO:0005739	Component	Mitochondrion	IBA	21873635
GO:0005739	Component	Mitochondrion	IDA
GO:0005759	Component	Mitochondrial matrix	TAS
GO:0005829	Component	Cytosol	IBA	21873635
GO:0006094	Process	Gluconeogenesis	IBA	21873635
GO:0006094	Process	Gluconeogenesis	IEA
GO:0006094	Process	Gluconeogenesis	TAS
GO:0006107	Process	Oxaloacetate metabolic process	IEA
GO:0006116	Process	NADH oxidation	IEA
GO:0019543	Process	Propionate catabolic process	IBA	21873635
GO:0030145	Function	Manganese ion binding	IBA	21873635
GO:0032024	Process	Positive regulation of insulin secretion	IEA
GO:0032496	Process	Response to lipopolysaccharide	IEA
GO:0032869	Process	Cellular response to insulin stimulus	IBA	21873635
GO:0033993	Process	Response to lipid	IBA	21873635
GO:0042594	Process	Response to starvation	IBA	21873635
GO:0046327	Process	Glycerol biosynthetic process from pyruvate	IBA	21873635
GO:0070365	Process	Hepatocyte differentiation	IBA	21873635
GO:0071333	Process	Cellular response to glucose stimulus	IBA	21873635
GO:0071356	Process	Cellular response to tumor necrosis factor	IEA
GO:0071549	Process	Cellular response to dexamethasone stimulus	IBA	21873635

Other IDs

• MIM: 614095
• HGNC: 8725
• e!Ensembl: ENSG00000100889

Protein

• UniProt ID: Q16822
• Protein name: Phosphoenolpyruvate carboxykinase [GTP], mitochondrial (PEPCK-M) (EC 4.1.1.32)
• Protein function: Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.
• Family and domains:

  Pfam

  Accession	ID	Position in sequence	Description	Type
  PF17297	PEPCK_N	46 → 274	Phosphoenolpyruvate carboxykinase N-terminal domain	Domain
  PF00821	PEPCK_GTP	278 → 637	Phosphoenolpyruvate carboxykinase C-terminal P-loop domain	Domain

• Sequence:

  MAALYRPGLRLNWHGLSPLGWPSCRSIQTLRVLSGDLGQLPTGIRDFVEHSARLCQPEGI
  HICDGTEAENTATLTLLEQQGLIRKLPKYNNCWLARTDPKDVARVESKTVIVTPSQRDTV
  PLPPGGARGQLGNWMSPADFQRAVDERFPGCMQGRTMYVLPFSMGPVGSPLSRIGVQLTD
  SAYVVASMRIMTRLGTPVLQALGDGDFVKCLHSVGQPLTGQGEPVSQWPCNPEKTLIGHV
  PDQREIISFGSGYGGNSLLGKKCFALRIASRLARDEGWLAEHMLILGITSPAGKKRYVAA
  AFPSACGKTNLAMMRPALPGWKVECVGDDIAWMRFDSEGRLRAINPENGFFGVAPGTSAT
  TNPNAMATIQSNTIFTNVAETSDGGVYWEGIDQPLPPGVTVTSWLGKPWKPGDKEPCAHP
  NSRFCAPARQCPIMDPAWEAPEGVPIDAIIFGGRRPKGVPLVYEAFNWRHGVFVGSAMRS
  ESTAAAEHKGKIIMHDPFAMRPFFGYNFGHYLEHWLSMEGRKGAQLPRIFHVNWFRRDEA
  GHFLWPGFGENARVLDWICRRLEGEDSARETPIGLVPKEGALDLSGLRAIDTTQLFSLPK
  DFWEQEVRDIRSYLTEQVNQDLPKEVLAELEALERRVHKM

• Sequence length: 640