MAP3K14 (mitogen-activated protein kinase kinase kinase 14)

Gene

• Entrez ID: 9020
• Gene name: Mitogen-activated protein kinase kinase kinase 14
• Gene symbol: MAP3K14
• Synonyms: FTDCR1B, HS, HSNIK, NIK
• Chromosome: 17
• Chromosome location: 17q21.31
• Summary: This gene encodes mitogen-activated protein kinase kinase kinase 14, which is a serine/threonine protein-kinase. This kinase binds to TRAF2 and stimulates NF-kappaB activity. It shares sequence similarity with several other MAPKK kinases. It participates in an NF-kappaB-inducing signalling cascade common to receptors of the tumour-necrosis/nerve-growth factor (TNF/NGF) family and to the interleukin-1 type-I receptor. [provided by RefSeq, Jul 2008]

miRNA

miRTarBase ID	miRNA	Experiments	Reference
MIRT020850	hsa-miR-155-5p	Reporter assay	20584899
MIRT020850	hsa-miR-155-5p	HITS-CLIP	22473208
MIRT052929	hsa-miR-520e	Luciferase reporter assay, qRT-PCR, Western blot	22105365
MIRT440286	hsa-miR-20a-5p	HITS-CLIP	22473208
MIRT440287	hsa-miR-106b-5p	HITS-CLIP	22473208
MIRT727251	hsa-miR-19b-3p	HITS-CLIP	22473208
MIRT727252	hsa-miR-19a-3p	HITS-CLIP	22473208
MIRT727253	hsa-miR-519d-3p	HITS-CLIP	22473208
MIRT727254	hsa-miR-93-5p	HITS-CLIP	22473208
MIRT727255	hsa-miR-20b-5p	HITS-CLIP	22473208
MIRT727256	hsa-miR-17-5p	HITS-CLIP	22473208
MIRT732593	hsa-miR-34a-5p	qRT-PCR	25864118

Transcription factors

Transcription factor	Regulation	Reference
HSF1	Unknown	19381566

Gene ontology (GO)

GO ID	Ontology	Definition	Evidence	Reference
GO:0000165	Process	MAPK cascade	IEA
GO:0000186	Process	Activation of MAPKK activity	IEA
GO:0001650	Component	Fibrillar center	IDA
GO:0004672	Function	Protein kinase activity	IDA	15001576
GO:0004674	Function	Protein serine/threonine kinase activity	EXP	9520446
GO:0004704	Function	NF-kappaB-inducing kinase activity	ISS
GO:0004709	Function	MAP kinase kinase kinase activity	IEA
GO:0005515	Function	Protein binding	IPI	9020361, 9520446, 10733566, 12884866, 14743216, 15208311, 16286467, 16498412, 17158868, 17237370, 22939624, 25036637, 25241761, 25416956, 28514442, 30833792, 31980649, 32296183, 32814053
GO:0005524	Function	ATP binding	IEA
GO:0005654	Component	Nucleoplasm	IDA
GO:0005829	Component	Cytosol	IDA
GO:0005829	Component	Cytosol	TAS
GO:0006468	Process	Protein phosphorylation	IEA
GO:0006955	Process	Immune response	ISS
GO:0007249	Process	I-kappaB kinase/NF-kappaB signaling	ISS
GO:0016301	Function	Kinase activity	TAS
GO:0033209	Process	Tumor necrosis factor-mediated signaling pathway	TAS
GO:0038061	Process	NIK/NF-kappaB signaling	TAS
GO:0043123	Process	Positive regulation of I-kappaB kinase/NF-kappaB signaling	IBA	21873635
GO:0043123	Process	Positive regulation of I-kappaB kinase/NF-kappaB signaling	IMP	10094049
GO:0043231	Component	Intracellular membrane-bounded organelle	IDA
GO:0051607	Process	Defense response to virus	IDA	21478870
GO:0071260	Process	Cellular response to mechanical stimulus	IEP	19593445

Other IDs

• MIM: 604655
• HGNC: 6853
• e!Ensembl: ENSG00000006062

Protein

• UniProt ID: Q99558
• Protein name: Mitogen-activated protein kinase kinase kinase 14 (EC 2.7.11.25) (NF-kappa-beta-inducing kinase) (HsNIK) (Serine/threonine-protein kinase NIK)
• Protein function: Lymphotoxin beta-activated kinase which seems to be exclusively involved in the activation of NF-kappa-B and its transcriptional activity. Promotes proteolytic processing of NFKB2/P100, which leads to activation of NF-kappa-B via the non-canonical pathway. Could act in a receptor-selective manner.
• PDB: 4DN5, 4G3D, 4IDT, 4IDV, 6WPP, 6Z1Q, 6Z1T
• Family and domains:

  Pfam

  Accession	ID	Position in sequence	Description	Type
  PF00069	Pkinase	401 → 649	Protein kinase domain	Domain

• Sequence:

  MAVMEMACPGAPGSAVGQQKELPKAKEKTPPLGKKQSSVYKLEAVEKSPVFCGKWEILND
  VITKGTAKEGSEAGPAAISIIAQAECENSQEFSPTFSERIFIAGSKQYSQSESLDQIPNN
  VAHATEGKMARVCWKGKRRSKARKKRKKKSSKSLAHAGVALAKPLPRTPEQESCTIPVQE
  DESPLGAPYVRNTPQFTKPLKEPGLGQLCFKQLGEGLRPALPRSELHKLISPLQCLNHVW
  KLHHPQDGGPLPLPTHPFPYSRLPHPFPFHPLQPWKPHPLESFLGKLACVDSQKPLPDPH
  LSKLACVDSPKPLPGPHLEPSCLSRGAHEKFSVEEYLVHALQGSVSSGQAHSLTSLAKTW
  AARGSRSREPSPKTEDNEGVLLTEKLKPVDYEYREEVHWATHQLRLGRGSFGEVHRMEDK
  QTGFQCAVKKVRLEVFRAEELMACAGLTSPRIVPLYGAVREGPWVNIFMELLEGGSLGQL
  VKEQGCLPEDRALYYLGQALEGLEYLHSRRILHGDVKADNVLLSSDGSHAALCDFGHAVC
  LQPDGLGKSLLTGDYIPGTETHMAPEVVLGRSCDAKVDVWSSCCMMLHMLNGCHPWTQFF
  RGPLCLKIASEPPPVREIPPSCAPLTAQAIQEGLRKEPIHRVSAAELGGKVNRALQQVGG
  LKSPWRGEYKEPRHPPPNQANYHQTLHAQPRELSPRAPGPRPAEETTGRAPKLQPPLPPE
  PPEPNKSPPLTLSKEESGMWEPLPLSSLEPAPARNPSSPERKATVPEQELQQLEIELFLN
  SLSQPFSLEEQEQILSCLSIDSLSLSDDSEKNPSKASQSSRDTLSSGVHSWSSQAEARSS
  SWNMVLARGRPTDTPSYFNGVKVQIQSLNGEHLHIREFHRVKVGDIATGISSQIPAAAFS
  LVTKDGQPVRYDMEVPDSGIDLQCTLAPDGSFAWSWRVKHGQLENRP

• Sequence length: 947