MAP2K3 (mitogen-activated protein kinase kinase 3)

Gene

• Entrez ID: 5606
• Gene name: Mitogen-activated protein kinase kinase 3
• Gene symbol: MAP2K3
• Synonyms: MAPKK3, MEK3, MKK3, PRKMK3, SAPKK-2, SAPKK2
• Chromosome: 17
• Chromosome location: 17p11.2
• Summary: The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphory

miRNA

miRTarBase ID	miRNA	Experiments	Reference
MIRT005426	hsa-miR-214-3p	GFP reporter assay, Microarray, qRT-PCR, Western blot	19859982
MIRT005426	hsa-miR-214-3p	GFP reporter assay, Microarray, qRT-PCR, Western blot	19859982
MIRT006772	hsa-miR-20a-5p	Luciferase reporter assay	22696064
MIRT006772	hsa-miR-20a-5p	Luciferase reporter assay	22696064
MIRT006772	hsa-miR-20a-5p	Luciferase reporter assay	22696064
MIRT006772	hsa-miR-20a-5p	Luciferase reporter assay	22696064
MIRT006772	hsa-miR-20a-5p	Luciferase reporter assay	22696064
MIRT022960	hsa-miR-124-3p	Microarray	18668037
MIRT005426	hsa-miR-214-3p	Reporter assay;qRT-PCR;Microarray;Other	19859982
MIRT028553	hsa-miR-30a-5p	Proteomics	18668040
MIRT076343	hsa-miR-21-5p	Immunohistochemistry, Luciferase reporter assay, Western blot	24112539
MIRT076343	hsa-miR-21-5p	Immunohistochemistry, Luciferase reporter assay, Western blot	24112539
MIRT440289	hsa-miR-218-5p	HITS-CLIP	23212916
MIRT144865	hsa-miR-19a-3p	HITS-CLIP	22473208
MIRT144866	hsa-miR-19b-3p	HITS-CLIP	22473208
MIRT440289	hsa-miR-218-5p	HITS-CLIP	23212916
MIRT005426	hsa-miR-214-3p	PAR-CLIP	26701625
MIRT735289	hsa-miR-28-3p	qRT-PCR	32425976
MIRT736107	mmu-miR-21a-5p	Luciferase reporter assay, Flow cytometry	31585536
MIRT1127959	hsa-miR-103a	CLIP-seq
MIRT1127960	hsa-miR-107	CLIP-seq
MIRT1127961	hsa-miR-1193	CLIP-seq
MIRT1127962	hsa-miR-1271	CLIP-seq
MIRT1127963	hsa-miR-1285	CLIP-seq
MIRT1127964	hsa-miR-1302	CLIP-seq
MIRT1127965	hsa-miR-15a	CLIP-seq
MIRT1127966	hsa-miR-15b	CLIP-seq
MIRT1127967	hsa-miR-16	CLIP-seq
MIRT1127968	hsa-miR-182	CLIP-seq
MIRT1127969	hsa-miR-1909	CLIP-seq
MIRT1127970	hsa-miR-195	CLIP-seq
MIRT1127971	hsa-miR-3122	CLIP-seq
MIRT1127972	hsa-miR-3136-3p	CLIP-seq
MIRT1127973	hsa-miR-3151	CLIP-seq
MIRT1127974	hsa-miR-3187-5p	CLIP-seq
MIRT1127975	hsa-miR-3614-5p	CLIP-seq
MIRT1127976	hsa-miR-3913-5p	CLIP-seq
MIRT1127977	hsa-miR-3943	CLIP-seq
MIRT1127978	hsa-miR-424	CLIP-seq
MIRT1127979	hsa-miR-4298	CLIP-seq
MIRT1127980	hsa-miR-4300	CLIP-seq
MIRT1127981	hsa-miR-4505	CLIP-seq
MIRT1127982	hsa-miR-4527	CLIP-seq
MIRT1127983	hsa-miR-4642	CLIP-seq
MIRT1127984	hsa-miR-4645-5p	CLIP-seq
MIRT1127985	hsa-miR-4673	CLIP-seq
MIRT1127986	hsa-miR-4688	CLIP-seq
MIRT1127987	hsa-miR-4708-3p	CLIP-seq
MIRT1127988	hsa-miR-486-3p	CLIP-seq
MIRT1127989	hsa-miR-497	CLIP-seq
MIRT1127990	hsa-miR-541	CLIP-seq
MIRT1127991	hsa-miR-612	CLIP-seq
MIRT1127992	hsa-miR-663b	CLIP-seq
MIRT1127993	hsa-miR-890	CLIP-seq
MIRT1127994	hsa-miR-9	CLIP-seq
MIRT1127995	hsa-miR-920	CLIP-seq
MIRT1127996	hsa-miR-96	CLIP-seq
MIRT1127997	hsa-miR-19a	CLIP-seq
MIRT1127998	hsa-miR-19b	CLIP-seq
MIRT1127999	hsa-miR-518a-5p	CLIP-seq
MIRT1128000	hsa-miR-527	CLIP-seq
MIRT1128001	hsa-miR-595	CLIP-seq
MIRT1128002	hsa-miR-648	CLIP-seq
MIRT1127965	hsa-miR-15a	CLIP-seq
MIRT1127966	hsa-miR-15b	CLIP-seq
MIRT1127967	hsa-miR-16	CLIP-seq
MIRT1127970	hsa-miR-195	CLIP-seq
MIRT1551116	hsa-miR-214	CLIP-seq
MIRT1127978	hsa-miR-424	CLIP-seq
MIRT1127989	hsa-miR-497	CLIP-seq
MIRT1551117	hsa-miR-708	CLIP-seq
MIRT2036343	hsa-miR-1262	CLIP-seq
MIRT2036344	hsa-miR-1286	CLIP-seq
MIRT1127964	hsa-miR-1302	CLIP-seq
MIRT2036345	hsa-miR-146b-3p	CLIP-seq
MIRT2036346	hsa-miR-199a-5p	CLIP-seq
MIRT2036347	hsa-miR-199b-5p	CLIP-seq
MIRT2036348	hsa-miR-21	CLIP-seq
MIRT2036349	hsa-miR-2392	CLIP-seq
MIRT2036350	hsa-miR-2467-3p	CLIP-seq
MIRT2036351	hsa-miR-3064-5p	CLIP-seq
MIRT1127971	hsa-miR-3122	CLIP-seq
MIRT1127973	hsa-miR-3151	CLIP-seq
MIRT2036352	hsa-miR-3169	CLIP-seq
MIRT2036353	hsa-miR-3170	CLIP-seq
MIRT2036354	hsa-miR-3173-5p	CLIP-seq
MIRT2036355	hsa-miR-3189-3p	CLIP-seq
MIRT2036356	hsa-miR-3192	CLIP-seq
MIRT2036357	hsa-miR-34a	CLIP-seq
MIRT2036358	hsa-miR-34c-5p	CLIP-seq
MIRT1127975	hsa-miR-3614-5p	CLIP-seq
MIRT2036359	hsa-miR-3652	CLIP-seq
MIRT2036360	hsa-miR-377	CLIP-seq
MIRT1127976	hsa-miR-3913-5p	CLIP-seq
MIRT1127977	hsa-miR-3943	CLIP-seq
MIRT2036361	hsa-miR-4287	CLIP-seq
MIRT2036362	hsa-miR-4292	CLIP-seq
MIRT2036363	hsa-miR-4294	CLIP-seq
MIRT1127979	hsa-miR-4298	CLIP-seq
MIRT1127980	hsa-miR-4300	CLIP-seq
MIRT2036364	hsa-miR-4302	CLIP-seq
MIRT2036365	hsa-miR-4313	CLIP-seq
MIRT2036366	hsa-miR-4323	CLIP-seq
MIRT2036367	hsa-miR-4326	CLIP-seq
MIRT2036368	hsa-miR-4430	CLIP-seq
MIRT2036369	hsa-miR-4469	CLIP-seq
MIRT2036370	hsa-miR-449a	CLIP-seq
MIRT2036371	hsa-miR-449b	CLIP-seq
MIRT1127981	hsa-miR-4505	CLIP-seq
MIRT2036372	hsa-miR-4515	CLIP-seq
MIRT2036373	hsa-miR-4640-5p	CLIP-seq
MIRT1127983	hsa-miR-4642	CLIP-seq
MIRT1127984	hsa-miR-4645-5p	CLIP-seq
MIRT1127985	hsa-miR-4673	CLIP-seq
MIRT2036374	hsa-miR-4676-5p	CLIP-seq
MIRT2036375	hsa-miR-4685-3p	CLIP-seq
MIRT2036376	hsa-miR-4691-5p	CLIP-seq
MIRT2036377	hsa-miR-4701-3p	CLIP-seq
MIRT2036378	hsa-miR-4726-5p	CLIP-seq
MIRT2036379	hsa-miR-4792	CLIP-seq
MIRT1127990	hsa-miR-541	CLIP-seq
MIRT2036380	hsa-miR-575	CLIP-seq
MIRT2036381	hsa-miR-590-5p	CLIP-seq
MIRT2036382	hsa-miR-596	CLIP-seq
MIRT2036383	hsa-miR-635	CLIP-seq
MIRT1128002	hsa-miR-648	CLIP-seq
MIRT1127992	hsa-miR-663b	CLIP-seq
MIRT2036384	hsa-miR-874	CLIP-seq
MIRT2036385	hsa-miR-885-3p	CLIP-seq
MIRT1127995	hsa-miR-920	CLIP-seq
MIRT2036386	hsa-miR-924	CLIP-seq
MIRT1127959	hsa-miR-103a	CLIP-seq
MIRT1127960	hsa-miR-107	CLIP-seq
MIRT1127961	hsa-miR-1193	CLIP-seq
MIRT2266582	hsa-miR-1202	CLIP-seq
MIRT2266583	hsa-miR-1245b-5p	CLIP-seq
MIRT2036343	hsa-miR-1262	CLIP-seq
MIRT1127963	hsa-miR-1285	CLIP-seq
MIRT2036344	hsa-miR-1286	CLIP-seq
MIRT2266584	hsa-miR-1291	CLIP-seq
MIRT2036345	hsa-miR-146b-3p	CLIP-seq
MIRT2266585	hsa-miR-1825	CLIP-seq
MIRT2266586	hsa-miR-1908	CLIP-seq
MIRT2266587	hsa-miR-1910	CLIP-seq
MIRT2036346	hsa-miR-199a-5p	CLIP-seq
MIRT2036347	hsa-miR-199b-5p	CLIP-seq
MIRT1127997	hsa-miR-19a	CLIP-seq
MIRT1127998	hsa-miR-19b	CLIP-seq
MIRT2036348	hsa-miR-21	CLIP-seq
MIRT1551116	hsa-miR-214	CLIP-seq
MIRT2036349	hsa-miR-2392	CLIP-seq
MIRT2036350	hsa-miR-2467-3p	CLIP-seq
MIRT2266588	hsa-miR-300	CLIP-seq
MIRT2266589	hsa-miR-3142	CLIP-seq
MIRT2036352	hsa-miR-3169	CLIP-seq
MIRT2036354	hsa-miR-3173-5p	CLIP-seq
MIRT1127974	hsa-miR-3187-5p	CLIP-seq
MIRT2036355	hsa-miR-3189-3p	CLIP-seq
MIRT2036357	hsa-miR-34a	CLIP-seq
MIRT2036358	hsa-miR-34c-5p	CLIP-seq
MIRT2266590	hsa-miR-3620	CLIP-seq
MIRT2266591	hsa-miR-3650	CLIP-seq
MIRT2266592	hsa-miR-381	CLIP-seq
MIRT2266593	hsa-miR-3926	CLIP-seq
MIRT1127977	hsa-miR-3943	CLIP-seq
MIRT2266594	hsa-miR-3972	CLIP-seq
MIRT2266595	hsa-miR-4264	CLIP-seq
MIRT2266596	hsa-miR-4271	CLIP-seq
MIRT2266597	hsa-miR-4291	CLIP-seq
MIRT2036362	hsa-miR-4292	CLIP-seq
MIRT2036364	hsa-miR-4302	CLIP-seq
MIRT2036365	hsa-miR-4313	CLIP-seq
MIRT2036366	hsa-miR-4323	CLIP-seq
MIRT2036367	hsa-miR-4326	CLIP-seq
MIRT2266598	hsa-miR-4493	CLIP-seq
MIRT2036370	hsa-miR-449a	CLIP-seq
MIRT2036371	hsa-miR-449b	CLIP-seq
MIRT1127981	hsa-miR-4505	CLIP-seq
MIRT2036372	hsa-miR-4515	CLIP-seq
MIRT2266599	hsa-miR-455-3p	CLIP-seq
MIRT2036373	hsa-miR-4640-5p	CLIP-seq
MIRT1127983	hsa-miR-4642	CLIP-seq
MIRT1127984	hsa-miR-4645-5p	CLIP-seq
MIRT2266600	hsa-miR-4666-3p	CLIP-seq
MIRT1127985	hsa-miR-4673	CLIP-seq
MIRT2036374	hsa-miR-4676-5p	CLIP-seq
MIRT2036376	hsa-miR-4691-5p	CLIP-seq
MIRT2266601	hsa-miR-4695-3p	CLIP-seq
MIRT2036377	hsa-miR-4701-3p	CLIP-seq
MIRT2266602	hsa-miR-4701-5p	CLIP-seq
MIRT1127987	hsa-miR-4708-3p	CLIP-seq
MIRT2266603	hsa-miR-4725-3p	CLIP-seq
MIRT2036378	hsa-miR-4726-5p	CLIP-seq
MIRT2266604	hsa-miR-4747-5p	CLIP-seq
MIRT2266605	hsa-miR-4755-3p	CLIP-seq
MIRT2266606	hsa-miR-4776-3p	CLIP-seq
MIRT2036379	hsa-miR-4792	CLIP-seq
MIRT1127999	hsa-miR-518a-5p	CLIP-seq
MIRT2266607	hsa-miR-520g	CLIP-seq
MIRT2266608	hsa-miR-520h	CLIP-seq
MIRT1128000	hsa-miR-527	CLIP-seq
MIRT2036380	hsa-miR-575	CLIP-seq
MIRT2266609	hsa-miR-581	CLIP-seq
MIRT2266610	hsa-miR-584	CLIP-seq
MIRT2266611	hsa-miR-588	CLIP-seq
MIRT2036381	hsa-miR-590-5p	CLIP-seq
MIRT1128001	hsa-miR-595	CLIP-seq
MIRT1127991	hsa-miR-612	CLIP-seq
MIRT2036383	hsa-miR-635	CLIP-seq
MIRT2266612	hsa-miR-644	CLIP-seq
MIRT2266613	hsa-miR-646	CLIP-seq
MIRT1128002	hsa-miR-648	CLIP-seq
MIRT2266614	hsa-miR-663	CLIP-seq
MIRT1127992	hsa-miR-663b	CLIP-seq
MIRT2266615	hsa-miR-665	CLIP-seq
MIRT2036384	hsa-miR-874	CLIP-seq
MIRT1127993	hsa-miR-890	CLIP-seq
MIRT2266616	hsa-miR-922	CLIP-seq
MIRT1127983	hsa-miR-4642	CLIP-seq
MIRT2036343	hsa-miR-1262	CLIP-seq
MIRT2036344	hsa-miR-1286	CLIP-seq
MIRT2266586	hsa-miR-1908	CLIP-seq
MIRT2036346	hsa-miR-199a-5p	CLIP-seq
MIRT2036347	hsa-miR-199b-5p	CLIP-seq
MIRT2036352	hsa-miR-3169	CLIP-seq
MIRT2036364	hsa-miR-4302	CLIP-seq
MIRT2036372	hsa-miR-4515	CLIP-seq
MIRT2036374	hsa-miR-4676-5p	CLIP-seq
MIRT2036377	hsa-miR-4701-3p	CLIP-seq
MIRT2036379	hsa-miR-4792	CLIP-seq
MIRT2036380	hsa-miR-575	CLIP-seq
MIRT1128002	hsa-miR-648	CLIP-seq
MIRT2266614	hsa-miR-663	CLIP-seq
MIRT2266582	hsa-miR-1202	CLIP-seq
MIRT2036343	hsa-miR-1262	CLIP-seq
MIRT2036344	hsa-miR-1286	CLIP-seq
MIRT2266584	hsa-miR-1291	CLIP-seq
MIRT2036345	hsa-miR-146b-3p	CLIP-seq
MIRT2266585	hsa-miR-1825	CLIP-seq
MIRT2266586	hsa-miR-1908	CLIP-seq
MIRT2036350	hsa-miR-2467-3p	CLIP-seq
MIRT2036352	hsa-miR-3169	CLIP-seq
MIRT2266594	hsa-miR-3972	CLIP-seq
MIRT2036372	hsa-miR-4515	CLIP-seq
MIRT2036377	hsa-miR-4701-3p	CLIP-seq
MIRT2266606	hsa-miR-4776-3p	CLIP-seq
MIRT2266614	hsa-miR-663	CLIP-seq

Gene ontology (GO)

GO ID	Ontology	Definition	Evidence	Reference
GO:0000187	Process	Activation of MAPK activity	IBA	21873635
GO:0000187	Process	Activation of MAPK activity	TAS
GO:0001817	Process	Regulation of cytokine production	IEA
GO:0004674	Function	Protein serine/threonine kinase activity	IEA
GO:0004708	Function	MAP kinase kinase activity	IBA	21873635
GO:0004713	Function	Protein tyrosine kinase activity	IEA
GO:0005515	Function	Protein binding	IPI	11980910, 17255949, 19805025, 20067578, 20173330, 21044950, 21771788, 23602568, 31980649
GO:0005524	Function	ATP binding	IEA
GO:0005654	Component	Nucleoplasm	TAS
GO:0005829	Component	Cytosol	TAS
GO:0006954	Process	Inflammatory response	IEA
GO:0007165	Process	Signal transduction	TAS	7839144
GO:0016020	Component	Membrane	HDA	19946888
GO:0018108	Process	Peptidyl-tyrosine phosphorylation	IEA
GO:0019901	Function	Protein kinase binding	IPI	11279118
GO:0035331	Process	Negative regulation of hippo signaling	IMP	27402810
GO:0035924	Process	Cellular response to vascular endothelial growth factor stimulus	IMP	22696064
GO:0038066	Process	P38MAPK cascade	IMP	22696064
GO:0043536	Process	Positive regulation of blood vessel endothelial cell migration	IMP	22696064
GO:0045860	Process	Positive regulation of protein kinase activity	IDA	11980910
GO:0045893	Process	Positive regulation of transcription, DNA-templated	IDA	11980910
GO:0060048	Process	Cardiac muscle contraction	IEA

Other IDs

• MIM: 602315
• HGNC: 6843
• e!Ensembl: ENSG00000034152

Protein

• UniProt ID: P46734
• Protein name: Dual specificity mitogen-activated protein kinase kinase 3 (MAP kinase kinase 3) (MAPKK 3) (EC 2.7.12.2) (MAPK/ERK kinase 3) (MEK 3) (Stress-activated protein kinase kinase 2) (SAPK kinase 2) (SAPKK-2) (SAPKK2)
• Protein function: Dual specificity kinase. Is activated by cytokines and environmental stress in vivo. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinase p38. Part of a signaling cascade that begins with the activati
• Family and domains:

  Pfam

  Accession	ID	Position in sequence	Description	Type
  PF00069	Pkinase	64 → 325	Protein kinase domain	Domain

• Sequence:

  MESPASSQPASMPQSKGKSKRKKDLRISCMSKPPAPNPTPPRNLDSRTFITIGDRNFEVE
  ADDLVTISELGRGAYGVVEKVRHAQSGTIMAVKRIRATVNSQEQKRLLMDLDINMRTVDC
  FYTVTFYGALFREGDVWICMELMDTSLDKFYRKVLDKNMTIPEDILGEIAVSIVRALEHL
  HSKLSVIHRDVKPSNVLINKEGHVKMCDFGISGYLVDSVAKTMDAGCKPYMAPERINPEL
  NQKGYNVKSDVWSLGITMIEMAILRFPYESWGTPFQQLKQVVEEPSPQLPADRFSPEFVD
  FTAQCLRKNPAERMSYLELMEHPFFTLHKTKKTDIAAFVKEILGEDS

• Sequence length: 347