GOT1 (glutamic-oxaloacetic transaminase 1)

Gene

• Entrez ID: 2805
• Gene name: Glutamic-oxaloacetic transaminase 1
• Gene symbol: GOT1
• Synonyms: AST1, ASTQTL1, GIG18, cAspAT, cCAT
• Chromosome: 10
• Chromosome location: 10q24.2
• Summary: Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. [provided by RefSeq, Jul 2008]

SNPs

SNP ID	Visualize variation	Clinical significance	Consequence
rs749913156	TTG>-	Pathogenic	Coding sequence variant, inframe deletion

miRNA

miRTarBase ID	miRNA	Experiments	Reference
MIRT039193	hsa-miR-769-5p	CLASH	23622248
MIRT497413	hsa-miR-7641	PAR-CLIP	22291592
MIRT497414	hsa-miR-4448	PAR-CLIP	22291592
MIRT497415	hsa-miR-549a	PAR-CLIP	22291592
MIRT497416	hsa-miR-205-5p	PAR-CLIP	22291592
MIRT497417	hsa-miR-1324	PAR-CLIP	22291592
MIRT645847	hsa-miR-3591-5p	HITS-CLIP	23824327
MIRT645848	hsa-miR-483-3p	HITS-CLIP	23824327
MIRT645849	hsa-miR-5003-5p	HITS-CLIP	23824327
MIRT645850	hsa-miR-8485	HITS-CLIP	23824327
MIRT727535	hsa-miR-181d-5p	HITS-CLIP	22473208
MIRT727536	hsa-miR-181c-5p	HITS-CLIP	22473208
MIRT727537	hsa-miR-181a-5p	HITS-CLIP	22473208
MIRT727538	hsa-miR-181b-5p	HITS-CLIP	22473208
MIRT738765	hsa-miR-200b-3p	PAR-CLIP	26701625
MIRT738777	hsa-miR-200c-3p	PAR-CLIP	26701625
MIRT743825	hsa-miR-429	PAR-CLIP	26701625
MIRT745939	hsa-miR-452-5p	PAR-CLIP	26701625
MIRT746894	hsa-miR-4676-3p	PAR-CLIP	26701625
MIRT750465	hsa-miR-548a-3p	PAR-CLIP	26701625
MIRT750582	hsa-miR-548ar-3p	PAR-CLIP	26701625
MIRT750645	hsa-miR-548az-3p	PAR-CLIP	26701625
MIRT750698	hsa-miR-548e-3p	PAR-CLIP	26701625
MIRT750708	hsa-miR-548f-3p	PAR-CLIP	26701625
MIRT750944	hsa-miR-5582-3p	PAR-CLIP	26701625
MIRT754329	hsa-miR-6740-5p	PAR-CLIP	26701625
MIRT760454	hsa-miR-892c-3p	PAR-CLIP	26701625

Gene ontology (GO)

GO ID	Ontology	Definition	Evidence	Reference
GO:0004069	Function	L-aspartate:2-oxoglutarate aminotransferase activity	IBA	21873635
GO:0004069	Function	L-aspartate:2-oxoglutarate aminotransferase activity	IDA	2182221, 2241899, 2731362, 6391741
GO:0004069	Function	L-aspartate:2-oxoglutarate aminotransferase activity	ISS
GO:0004609	Function	Phosphatidylserine decarboxylase activity	IEA
GO:0005634	Component	Nucleus	HDA	21630459
GO:0005654	Component	Nucleoplasm	IDA
GO:0005737	Component	Cytoplasm	IDA	7060339
GO:0005764	Component	Lysosome	IEA
GO:0005829	Component	Cytosol	IBA	21873635
GO:0005829	Component	Cytosol	IDA
GO:0005829	Component	Cytosol	TAS
GO:0006094	Process	Gluconeogenesis	TAS
GO:0006103	Process	2-oxoglutarate metabolic process	ISS
GO:0006107	Process	Oxaloacetate metabolic process	IEA
GO:0006114	Process	Glycerol biosynthetic process	ISS
GO:0006531	Process	Aspartate metabolic process	ISS
GO:0006532	Process	Aspartate biosynthetic process	IBA	21873635
GO:0006533	Process	Aspartate catabolic process	IDA	2241899
GO:0006536	Process	Glutamate metabolic process	ISS
GO:0007219	Process	Notch signaling pathway	IEA
GO:0008652	Process	Cellular amino acid biosynthetic process	TAS
GO:0009743	Process	Response to carbohydrate	IEA
GO:0019550	Process	Glutamate catabolic process to aspartate	IEA
GO:0019551	Process	Glutamate catabolic process to 2-oxoglutarate	IEA
GO:0030170	Function	Pyridoxal phosphate binding	IEA
GO:0030511	Process	Positive regulation of transforming growth factor beta receptor signaling pathway	IEA
GO:0031406	Function	Carboxylic acid binding	IEA
GO:0032869	Process	Cellular response to insulin stimulus	IEP	8396422
GO:0032966	Process	Negative regulation of collagen biosynthetic process	IEA
GO:0035902	Process	Response to immobilization stress	IEA
GO:0043679	Component	Axon terminus	IEA
GO:0046686	Process	Response to cadmium ion	IEA
GO:0047801	Function	L-cysteine:2-oxoglutarate aminotransferase activity	ISS
GO:0051384	Process	Response to glucocorticoid	IEP	8396422
GO:0051481	Process	Negative regulation of cytosolic calcium ion concentration	IEA
GO:0051902	Process	Negative regulation of mitochondrial depolarization	IEA
GO:0055089	Process	Fatty acid homeostasis	IEA
GO:0060290	Process	Transdifferentiation	IEA
GO:0070062	Component	Extracellular exosome	HDA	19056867, 19199708, 20458337, 23533145
GO:0071260	Process	Cellular response to mechanical stimulus	IEA
GO:1990267	Process	Response to transition metal nanoparticle	IEA

Other IDs

• MIM: 138180
• HGNC: 4432
• e!Ensembl: ENSG00000120053

Protein

• UniProt ID: P17174
• Protein name: Aspartate aminotransferase, cytoplasmic (cAspAT) (EC 2.6.1.1) (EC 2.6.1.3) (Cysteine aminotransferase, cytoplasmic) (Cysteine transaminase, cytoplasmic) (cCAT) (Glutamate oxaloacetate transaminase 1) (Transaminase A)
• Protein function: Biosynthesis of L-glutamate from L-aspartate or L-cysteine. Important regulator of levels of glutamate, the major excitatory neurotransmitter of the vertebrate central nervous system. Acts as a scavenger of glutamate in brain neuroprotection. The aspartate aminotransferase activity is involved in hepatic glucose synthesis during development and in adipocyte glyceroneogenesis. Using L-cysteine as substrate, regulates levels of mercaptopyruvate, an important source of hydrogen sulfide. Mercaptopyruvate is converted into H(2)S via the action of 3-mercaptopyruvate sulfurtransferase (3MST). Hydrogen sulfide is an important synaptic modulator and neuroprotectant in the brain.
• PDB: 3II0, 3WZF, 6DNA, 6DNB, 6DND, 6LIG
• Family and domains:

  Pfam

  Accession	ID	Position in sequence	Description	Type
  PF00155	Aminotran_1_2	31 → 405	Aminotransferase class I and II	Domain

• Sequence:

  MAPPSVFAEVPQAQPVLVFKLTADFREDPDPRKVNLGVGAYRTDDCHPWVLPVVKKVEQK
  IANDNSLNHEYLPILGLAEFRSCASRLALGDDSPALKEKRVGGVQSLGGTGALRIGADFL
  ARWYNGTNNKNTPVYVSSPTWENHNAVFSAAGFKDIRSYRYWDAEKRGLDLQGFLNDLEN
  APEFSIVVLHACAHNPTGIDPTPEQWKQIASVMKHRFLFPFFDSAYQGFASGNLERDAWA
  IRYFVSEGFEFFCAQSFSKNFGLYNERVGNLTVVGKEPESILQVLSQMEKIVRITWSNPP
  AQGARIVASTLSNPELFEEWTGNVKTMADRILTMRSELRARLEALKTPGTWNHITDQIGM
  FSFTGLNPKQVEYLVNEKHIYLLPSGRINVSGLTTKNLDYVATSIHEAVTKIQ

• Sequence length: 413