GLS2 (glutaminase 2)

Gene

• Entrez ID: 27165
• Gene name: Glutaminase 2
• Gene symbol: GLS2
• Synonyms: GA, GLS, LGA, hLGA
• Chromosome: 12
• Chromosome location: 12q13.3
• Summary: The protein encoded by this gene is a mitochondrial phosphate-activated glutaminase that catalyzes the hydrolysis of glutamine to stoichiometric amounts of glutamate and ammonia. Originally thought to be liver-specific, this protein has been found in other tissues as well. Alternative splicing results in multiple transcript variants that encode different isoforms. [provided by RefSeq, Jul 2013]

miRNA

miRTarBase ID	miRNA	Experiments	Reference
MIRT016977	hsa-miR-335-5p	Microarray	18185580
MIRT734798	hsa-miR-16-5p	Luciferase reporter assay, qRT-PCR, Western blot	26373319

Gene ontology (GO)

GO ID	Ontology	Definition	Evidence	Reference
GO:0004359	Function	Glutaminase activity	IBA	21873635
GO:0005515	Function	Protein binding	IPI	11163757, 32296183
GO:0005739	Component	Mitochondrion	IDA	20351271
GO:0005759	Component	Mitochondrial matrix	TAS
GO:0006520	Process	Cellular amino acid metabolic process	TAS	10620514
GO:0006537	Process	Glutamate biosynthetic process	IBA	21873635
GO:0006543	Process	Glutamine catabolic process	IBA	21873635
GO:0008652	Process	Cellular amino acid biosynthetic process	TAS
GO:0014047	Process	Glutamate secretion	TAS
GO:0042981	Process	Regulation of apoptotic process	IMP	20351271
GO:0072593	Process	Reactive oxygen species metabolic process	IMP	20351271

Other IDs

• MIM: 606365
• HGNC: 29570
• e!Ensembl: ENSG00000135423

Protein

• UniProt ID: Q9UI32
• Protein name: Glutaminase liver isoform, mitochondrial (GLS) (EC 3.5.1.2) (L-glutaminase) (L-glutamine amidohydrolase)
• Protein function: Plays an important role in the regulation of glutamine catabolism. Promotes mitochondrial respiration and increases ATP generation in cells by catalyzing the synthesis of glutamate and alpha-ketoglutarate. Increases cellular anti-oxidant function via NADH and glutathione production. May play a role in preventing tumor proliferation.
• PDB: 4BQM, 5U0K
• Family and domains:

  Pfam

  Accession	ID	Position in sequence	Description	Type
  PF17959	EF-hand_14	72 → 155	EF-hand domain	Domain
  PF04960	Glutaminase	177 → 463	Glutaminase	Domain
  PF12796	Ank_2	484 → 582	Ankyrin repeats (3 copies)	Repeat

• Sequence:

  MRSMKALQKALSRAGSHCGRGGWGHPSRSPLLGGGVRHHLSEAAAQGRETPHSHQPQHQD
  HDSSESGMLSRLGDLLFYTIAEGQERIPIHKFTTALKATGLQTSDPRLRDCMSEMHRVVQ
  ESSSGGLLDRDLFRKCVSSNIVLLTQAFRKKFVIPDFEEFTGHVDRIFEDVKELTGGKVA
  AYIPQLAKSNPDLWGVSLCTVDGQRHSVGHTKIPFCLQSCVKPLTYAISISTLGTDYVHK
  FVGKEPSGLRYNKLSLNEEGIPHNPMVNAGAIVVSSLIKMDCNKAEKFDFVLQYLNKMAG
  NEYMGFSNATFQSEKETGDRNYAIGYYLKEKKCFPKGVDMMAALDLYFQLCSVEVTCESG
  SVMAATLANGGICPITGESVLSAEAVRNTLSLMHSCGMYDFSGQFAFHVGLPAKSAVSGA
  ILLVVPNVMGMMCLSPPLDKLGNSHRGTSFCQKLVSLFNFHNYDNLRHCARKLDPRREGA
  EIRNKTVVNLLFAAYSGDVSALRRFALSAMDMEQKDYDSRTALHVAAAEGHIEVVKFLIE
  ACKVNPFAKDRWGNIPLDDAVQFNHLEVVKLLQDYQDSYTLSETQAEAAAEALSKENLES
  MV

• Sequence length: 602