ATP2B1 (ATPase plasma membrane Ca2+ transporting 1)

Gene

• Entrez ID: 490
• Gene name: ATPase plasma membrane Ca2+ transporting 1
• Gene symbol: ATP2B1
• Synonyms: PMCA1, PMCA1kb
• Chromosome: 12
• Chromosome location: 12q21.33
• Summary: The protein encoded by this gene belongs to the family of P-type primary ion transport ATPases characterized by the formation of an aspartyl phosphate intermediate during the reaction cycle. These enzymes remove bivalent calcium ions from eukaryotic cells against very large concentration gradients and play a critical role in intracellular calcium homeostasis. The mammalian plasma membrane calcium ATPase isoforms are encoded by at least four separate genes and the diversity of these enzymes is further increased by alternative splicing of transcripts. The expression of different isoforms and splice variants is regulated in a developmental, tissue- and cell type-specific manner, suggesting that these pumps are functionally adapted to the physiological needs of particular cells and tissues. This gene encodes the plasma membrane calcium ATPase isoform 1. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

miRNA

miRTarBase ID	miRNA	Experiments	Reference
MIRT005818	hsa-miR-204-5p	Microarray	21282569
MIRT039645	hsa-miR-615-3p	CLASH	23622248
MIRT052579	hsa-let-7a-5p	CLASH	23622248
MIRT136366	hsa-miR-150-5p	HITS-CLIP	19536157
MIRT136399	hsa-miR-4713-5p	HITS-CLIP	19536157
MIRT136414	hsa-miR-6892-3p	HITS-CLIP	19536157
MIRT384905	hsa-miR-6817-3p	HITS-CLIP	19536157
MIRT384906	hsa-miR-6873-3p	HITS-CLIP	19536157
MIRT384907	hsa-miR-7110-3p	HITS-CLIP	19536157
MIRT441150	hsa-miR-20a-5p	HITS-CLIP	22473208
MIRT441151	hsa-miR-106b-5p	HITS-CLIP	22473208
MIRT441152	hsa-miR-142-3p	HITS-CLIP	22473208
MIRT561119	hsa-miR-4465	PAR-CLIP	20371350
MIRT561120	hsa-miR-498	PAR-CLIP	20371350
MIRT561121	hsa-miR-26b-5p	PAR-CLIP	20371350
MIRT561122	hsa-miR-26a-5p	PAR-CLIP	20371350
MIRT561123	hsa-miR-1297	PAR-CLIP	20371350
MIRT561124	hsa-miR-5697	PAR-CLIP	20371350
MIRT561125	hsa-miR-513c-3p	PAR-CLIP	20371350
MIRT561126	hsa-miR-513a-3p	PAR-CLIP	20371350
MIRT561127	hsa-miR-4282	PAR-CLIP	20371350
MIRT561128	hsa-miR-3606-3p	PAR-CLIP	20371350
MIRT724522	hsa-miR-6749-3p	HITS-CLIP	19536157
MIRT724523	hsa-miR-642a-5p	HITS-CLIP	19536157
MIRT724524	hsa-miR-6845-3p	HITS-CLIP	19536157
MIRT724525	hsa-miR-4469	HITS-CLIP	19536157
MIRT724526	hsa-miR-2116-3p	HITS-CLIP	19536157
MIRT724527	hsa-miR-532-3p	HITS-CLIP	19536157
MIRT724528	hsa-miR-6769b-3p	HITS-CLIP	19536157
MIRT724529	hsa-miR-4723-3p	HITS-CLIP	19536157
MIRT724530	hsa-miR-3183	HITS-CLIP	19536157
MIRT724531	hsa-miR-2276-5p	HITS-CLIP	19536157
MIRT724532	hsa-miR-483-3p	HITS-CLIP	19536157
MIRT728252	hsa-miR-181d-5p	HITS-CLIP	22473208
MIRT728253	hsa-miR-181c-5p	HITS-CLIP	22473208
MIRT728254	hsa-miR-181b-5p	HITS-CLIP	22473208
MIRT728255	hsa-miR-519d-3p	HITS-CLIP	22473208
MIRT728256	hsa-miR-181a-5p	HITS-CLIP	22473208
MIRT728257	hsa-miR-93-5p	HITS-CLIP	22473208
MIRT728258	hsa-miR-20b-5p	HITS-CLIP	22473208
MIRT728259	hsa-miR-17-5p	HITS-CLIP	22473208

Gene ontology (GO)

GO ID	Ontology	Definition	Evidence	Reference
GO:0001772	Component	Immunological synapse	ISS
GO:0001818	Process	Negative regulation of cytokine production	ISS
GO:0003056	Process	Regulation of vascular associated smooth muscle contraction	ISS
GO:0003407	Process	Neural retina development	IEA
GO:0005388	Function	Calcium transmembrane transporter activity, phosphorylative mechanism	IBA	21873635
GO:0005515	Function	Protein binding	IPI	18029012, 22084111, 30126976, 30190470
GO:0005516	Function	Calmodulin binding	IEA
GO:0005524	Function	ATP binding	IEA
GO:0005654	Component	Nucleoplasm	IDA
GO:0005886	Component	Plasma membrane	IDA	18029012
GO:0005886	Component	Plasma membrane	TAS
GO:0005887	Component	Integral component of plasma membrane	IBA	21873635
GO:0006874	Process	Cellular calcium ion homeostasis	IBA	21873635
GO:0007420	Process	Brain development	IEA
GO:0007568	Process	Aging	IEA
GO:0008217	Process	Regulation of blood pressure	ISS
GO:0009409	Process	Response to cold	IEA
GO:0009898	Component	Cytoplasmic side of plasma membrane	IEA
GO:0015085	Function	Calcium ion transmembrane transporter activity	IDA	18029012
GO:0016020	Component	Membrane	HDA	19946888
GO:0016323	Component	Basolateral plasma membrane	ISS
GO:0016324	Component	Apical plasma membrane	IEA
GO:0016887	Function	ATPase activity	IDA	30190470
GO:0019829	Function	ATPase-coupled cation transmembrane transporter activity	IBA	21873635
GO:0030165	Function	PDZ domain binding	IBA	21873635
GO:0030501	Process	Positive regulation of bone mineralization	ISS
GO:0032591	Component	Dendritic spine membrane	IEA
GO:0032809	Component	Neuronal cell body membrane	IEA
GO:0034220	Process	Ion transmembrane transport	TAS
GO:0043231	Component	Intracellular membrane-bounded organelle	IBA	21873635
GO:0045121	Component	Membrane raft	IEA
GO:0046872	Function	Metal ion binding	IEA
GO:0051480	Process	Regulation of cytosolic calcium ion concentration	IBA	21873635
GO:0051480	Process	Regulation of cytosolic calcium ion concentration	IDA	18029012
GO:0051481	Process	Negative regulation of cytosolic calcium ion concentration	IMP	29104511
GO:0051481	Process	Negative regulation of cytosolic calcium ion concentration	ISS
GO:0051928	Process	Positive regulation of calcium ion transport	ISS
GO:0070062	Component	Extracellular exosome	HDA	20458337
GO:0071305	Process	Cellular response to vitamin D	IEA
GO:0071386	Process	Cellular response to corticosterone stimulus	IEA
GO:0098978	Component	Glutamatergic synapse	IDA	18029012
GO:0098978	Component	Glutamatergic synapse	IMP	18029012
GO:0098982	Component	GABA-ergic synapse	IDA	18029012
GO:0098982	Component	GABA-ergic synapse	IMP	18029012
GO:0099059	Component	Integral component of presynaptic active zone membrane	IEA
GO:0099509	Process	Regulation of presynaptic cytosolic calcium ion concentration	IEA
GO:1900076	Process	Regulation of cellular response to insulin stimulus	IMP	29104511
GO:1903779	Process	Regulation of cardiac conduction	TAS
GO:1905056	Function	Calcium-transporting ATPase activity involved in regulation of presynaptic cytosolic calcium ion concentration	IDA	18029012
GO:1905056	Function	Calcium-transporting ATPase activity involved in regulation of presynaptic cytosolic calcium ion concentration	IMP	18029012
GO:1990034	Process	Calcium ion export across plasma membrane	IDA	18029012

Other IDs

• MIM: 108731
• HGNC: 814
• e!Ensembl: ENSG00000070961

Protein

• UniProt ID: P20020
• Protein name: Plasma membrane calcium-transporting ATPase 1 (EC 7.2.2.10) (Plasma membrane calcium ATPase isoform 1) (PMCA1) (Plasma membrane calcium pump isoform 1)
• Protein function: Catalyzes the hydrolysis of ATP coupled with the transport of calcium from the cytoplasm to the extracellular space thereby maintaining intracellular calcium homeostasis. Plays a role in blood pressure regulation through regulation of intracellular calcium concentration and nitric oxide production leading to regulation of vascular smooth muscle cells vasoconstriction. Positively regulates bone mineralization through absorption of calcium from the intestine. Plays dual roles in osteoclast differentiation and survival by regulating RANKL-induced calcium oscillations in preosteoclasts and mediating calcium extrusion in mature osteoclasts (By similarity). Regulates insulin sensitivity through calcium/calmodulin signaling pathway by regulating AKT1 activation and NOS3 activation in endothelial cells (PubMed:29104511). May play a role in synaptic transmission by modulating calcium and proton dynamics at the synaptic vesicles.
• PDB: 6A69
• Family and domains:

  Pfam

  Accession	ID	Position in sequence	Description	Type
  PF00690	Cation_ATPase_N	51 → 121	Cation transporter/ATPase, N-terminus	Domain
  PF00122	E1-E2_ATPase	189 → 307		Family
  PF00122	E1-E2_ATPase	347 → 453		Family
  PF13246	Cation_ATPase	482 → 614		Family
  PF00702	Hydrolase	658 → 809		Domain
  PF00689	Cation_ATPase_C	879 → 1061	Cation transporting ATPase, C-terminus	Family
  PF12424	ATP_Ca_trans_C	1103 → 1149	Plasma membrane calcium transporter ATPase C terminal	Family

• Sequence:

  MGDMANNSVAYSGVKNSLKEANHDGDFGITLAELRALMELRSTDALRKIQESYGDVYGIC
  TKLKTSPNEGLSGNPADLERREAVFGKNFIPPKKPKTFLQLVWEALQDVTLIILEIAAIV
  SLGLSFYQPPEGDNALCGEVSVGEEEGEGETGWIEGAAILLSVVCVVLVTAFNDWSKEKQ
  FRGLQSRIEQEQKFTVIRGGQVIQIPVADITVGDIAQVKYGDLLPADGILIQGNDLKIDE
  SSLTGESDHVKKSLDKDPLLLSGTHVMEGSGRMVVTAVGVNSQTGIIFTLLGAGGEEEEK
  KDEKKKEKKNKKQDGAIENRNKAKAQDGAAMEMQPLKSEEGGDGDEKDKKKANLPKKEKS
  VLQGKLTKLAVQIGKAGLLMSAITVIILVLYFVIDTFWVQKRPWLAECTPIYIQYFVKFF
  IIGVTVLVVAVPEGLPLAVTISLAYSVKKMMKDNNLVRHLDACETMGNATAICSDKTGTL
  TMNRMTVVQAYINEKHYKKVPEPEAIPPNILSYLVTGISVNCAYTSKILPPEKEGGLPRH
  VGNKTECALLGLLLDLKRDYQDVRNEIPEEALYKVYTFNSVRKSMSTVLKNSDGSYRIFS
  KGASEIILKKCFKILSANGEAKVFRPRDRDDIVKTVIEPMASEGLRTICLAFRDFPAGEP
  EPEWDNENDIVTGLTCIAVVGIEDPVRPEVPDAIKKCQRAGITVRMVTGDNINTARAIAT
  KCGILHPGEDFLCLEGKDFNRRIRNEKGEIEQERIDKIWPKLRVLARSSPTDKHTLVKGI
  IDSTVSDQRQVVAVTGDGTNDGPALKKADVGFAMGIAGTDVAKEASDIILTDDNFTSIVK
  AVMWGRNVYDSISKFLQFQLTVNVVAVIVAFTGACITQDSPLKAVQMLWVNLIMDTLASL
  ALATEPPTESLLLRKPYGRNKPLISRTMMKNILGHAFYQLVVVFTLLFAGEKFFDIDSGR
  NAPLHAPPSEHYTIVFNTFVLMQLFNEINARKIHGERNVFEGIFNNAIFCTIVLGTFVVQ
  IIIVQFGGKPFSCSELSIEQWLWSIFLGMGTLLWGQLISTIPTSRLKFLKEAGHGTQKEE
  IPEEELAEDVEEIDHAERELRRGQILWFRGLNRIQTQIRVVNAFRSSLYEGLEKPESRSS
  IHNFMTHPEFRIEDSEPHIPLIDDTDAEDDAPTKRNSSPPPSPNKNNNAVDSGIHLTIEM
  NKSATSSSPGSPLHSLETSL

• Sequence length: 1220