ARAF (A-Raf proto-oncogene, serine/threonine kinase)

Gene

• Entrez ID: 369
• Gene name: A-Raf proto-oncogene, serine/threonine kinase
• Gene symbol: ARAF
• Synonyms: A-RAF, ARAF1, PKS2, RAFA1
• Chromosome: X
• Chromosome location: Xp11.3
• Summary: This proto-oncogene belongs to the RAF subfamily of the Ser/Thr protein kinase family, and maybe involved in cell growth and development. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jan 2012]

SNPs

SNP ID	Visualize variation	Clinical significance	Consequence
rs1057519786	C>G,T	Not-provided, likely-pathogenic	Missense variant, coding sequence variant, genic downstream transcript variant
rs1057519876	T>G	Likely-pathogenic	Missense variant, coding sequence variant, genic downstream transcript variant

miRNA

miRTarBase ID	miRNA	Experiments	Reference
MIRT004929	hsa-miR-124-3p	Microarray	15685193
MIRT004929	hsa-miR-124-3p	Microarray	18668037
MIRT043358	hsa-miR-331-3p	CLASH	23622248

Gene ontology (GO)

GO ID	Ontology	Definition	Evidence	Reference
GO:0000165	Process	MAPK cascade	TAS
GO:0000186	Process	Activation of MAPKK activity	IEA
GO:0004672	Function	Protein kinase activity	IBA	21873635
GO:0004674	Function	Protein serine/threonine kinase activity	IDA	19667065
GO:0004709	Function	MAP kinase kinase kinase activity	IEA
GO:0005515	Function	Protein binding	IPI	11909642, 12620389, 15778465, 17979178, 19933840, 20052757, 20130576, 20936779, 21903422, 21988832, 22939624, 24441586, 24746704, 25036637, 25241761, 25852190, 28514442, 31980649
GO:0005524	Function	ATP binding	IEA
GO:0005575	Component	Cellular_component	ND
GO:0005739	Component	Mitochondrion	IBA	21873635
GO:0005829	Component	Cytosol	IBA	21873635
GO:0005829	Component	Cytosol	TAS
GO:0006464	Process	Cellular protein modification process	TAS	3029685
GO:0006468	Process	Protein phosphorylation	IEA
GO:0032006	Process	Regulation of TOR signaling	IMP	22609986
GO:0032434	Process	Regulation of proteasomal ubiquitin-dependent protein catabolic process	IMP	22609986
GO:0033138	Process	Positive regulation of peptidyl-serine phosphorylation	IDA	19667065
GO:0043066	Process	Negative regulation of apoptotic process	IDA	19667065
GO:0046872	Function	Metal ion binding	IEA
GO:0106310	Function	Protein serine kinase activity	IEA
GO:0106311	Function	Protein threonine kinase activity	IEA

Other IDs

• MIM: 311010
• HGNC: 646
• e!Ensembl: ENSG00000078061

Protein

• UniProt ID: P10398
• Protein name: Serine/threonine-protein kinase A-Raf (EC 2.7.11.1) (Proto-oncogene A-Raf) (Proto-oncogene A-Raf-1) (Proto-oncogene Pks)
• Protein function: Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May also regulate the TOR signaling cascade. ; [Isoform 2]: Serves as a positive regulator of myogenic differentiation by inducing cell cycle arrest, the expression of myogenin and other muscle-specific proteins, and myotube formation.
• PDB: 1WXM, 2MSE
• Family and domains:

  Pfam

  Accession	ID	Position in sequence	Description	Type
  PF02196	RBD	20 → 89	Raf-like Ras-binding domain	Domain
  PF00130	C1_1	99 → 147	Phorbol esters/diacylglycerol binding domain (C1 domain)	Domain
  PF07714	PK_Tyr_Ser-Thr	310 → 567	Protein tyrosine and serine/threonine kinase	Domain

• Sequence:

  MEPPRGPPANGAEPSRAVGTVKVYLPNKQRTVVTVRDGMSVYDSLDKALKVRGLNQDCCV
  VYRLIKGRKTVTAWDTAIAPLDGEELIVEVLEDVPLTMHNFVRKTFFSLAFCDFCLKFLF
  HGFRCQTCGYKFHQHCSSKVPTVCVDMSTNRQQFYHSVQDLSGGSRQHEAPSNRPLNELL
  TPQGPSPRTQHCDPEHFPFPAPANAPLQRIRSTSTPNVHMVSTTAPMDSNLIQLTGQSFS
  TDAAGSRGGSDGTPRGSPSPASVSSGRKSPHSKSPAEQRERKSLADDKKKVKNLGYRDSG
  YYWEVPPSEVQLLKRIGTGSFGTVFRGRWHGDVAVKVLKVSQPTAEQAQAFKNEMQVLRK
  TRHVNILLFMGFMTRPGFAIITQWCEGSSLYHHLHVADTRFDMVQLIDVARQTAQGMDYL
  HAKNIIHRDLKSNNIFLHEGLTVKIGDFGLATVKTRWSGAQPLEQPSGSVLWMAAEVIRM
  QDPNPYSFQSDVYAYGVVLYELMTGSLPYSHIGCRDQIIFMVGRGYLSPDLSKISSNCPK
  AMRRLLSDCLKFQREERPLFPQILATIELLQRSLPKIERSASEPSLHRTQADELPACLLS
  AARLVP

• Sequence length: 606