AFDN (afadin, adherens junction formation factor)

Gene

• Entrez ID: 4301
• Gene name: Afadin, adherens junction formation factor
• Gene symbol: AFDN
• Synonyms: AF6, MLL-AF6, MLLT4, l-afadin
• Chromosome: 6
• Chromosome location: 6q27
• Summary: This gene encodes a multi-domain protein involved in signaling and organization of cell junctions during embryogenesis. It has also been identified as the fusion partner of acute lymphoblastic leukemia (ALL-1) gene, involved in acute myeloid leukemias wit

Gene ontology (GO)

GO ID	Ontology	Definition	Evidence	Reference
GO:0005515	Function	Protein binding	IPI	17491594, 19461049, 22891260, 23750010, 23885123, 27815408, 30463011
GO:0005654	Component	Nucleoplasm	IDA
GO:0005829	Component	Cytosol	IDA
GO:0005829	Component	Cytosol	TAS
GO:0005886	Component	Plasma membrane	IDA
GO:0005911	Component	Cell-cell junction	IDA	19461049
GO:0005912	Component	Adherens junction	IBA	21873635
GO:0007155	Process	Cell adhesion	TAS	10856295
GO:0007165	Process	Signal transduction	IEA
GO:0007267	Process	Cell-cell signaling	TAS	8242616
GO:0008022	Function	Protein C-terminus binding	TAS	10856295
GO:0010628	Process	Positive regulation of gene expression	IMP	25893857
GO:0016607	Component	Nuclear speck	IDA
GO:0022409	Process	Positive regulation of cell-cell adhesion	IMP	25893857
GO:0030054	Component	Cell junction	IDA
GO:0030336	Process	Negative regulation of cell migration	IMP	21478912
GO:0031267	Function	Small GTPase binding	IPI	23885123
GO:0032880	Process	Regulation of protein localization	IBA	21873635
GO:0034332	Process	Adherens junction organization	TAS
GO:0043547	Process	Positive regulation of GTPase activity	IMP	23885123
GO:0044291	Component	Cell-cell contact zone	IDA	16882694
GO:0044331	Process	Cell-cell adhesion mediated by cadherin	ISS	23990464
GO:0045296	Function	Cadherin binding	HDA	25468996
GO:0046930	Component	Pore complex	IMP	30463011
GO:0046931	Process	Pore complex assembly	IMP	30463011
GO:0050839	Function	Cell adhesion molecule binding	IBA	21873635
GO:0051015	Function	Actin filament binding	IDA	16882694
GO:0061951	Process	Establishment of protein localization to plasma membrane	ISS	23990464
GO:0070160	Component	Tight junction	IDA	27815408
GO:0070830	Process	Bicellular tight junction assembly	IMP	27815408
GO:0090557	Process	Establishment of endothelial intestinal barrier	IMP	23885123
GO:2000049	Process	Positive regulation of cell-cell adhesion mediated by cadherin	IMP	16882694

Other IDs

• MIM: 159559
• HGNC: 7137
• e!Ensembl: ENSG00000130396

Protein

• UniProt ID: P55196
• Protein name: Afadin (ALL1-fused gene from chromosome 6 protein) (Protein AF-6) (Afadin adherens junction formation factor)
• Protein function: Belongs to an adhesion system, probably together with the E-cadherin-catenin system, which plays a role in the organization of homotypic, interneuronal and heterotypic cell-cell adherens junctions (AJs) (By similarity). Nectin- and actin-filamen
• PDB: 1T2M, 1XZ9, 2AIN, 2EXG, 5A6C, 7QCR
• Family and domains:

  Pfam

  Accession	ID	Position in sequence	Description	Type
  PF00788	RA	39 → 133	Ras association (RalGDS/AF-6) domain	Domain
  PF00788	RA	246 → 348	Ras association (RalGDS/AF-6) domain	Domain
  PF00498	FHA	426 → 492	FHA domain	Family
  PF01843	DIL	785 → 889	DIL domain	Family
  PF00595	PDZ	1008 → 1090	PDZ domain	Domain

• Sequence:

  MSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCI
  RVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGERRLDIDEKPLVVQLNWNKD
  DREGRFVLKNENDAIPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEALRQA
  SDKDDRPFQGEDVENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSS
  DGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARV
  MLPPGAQHSDEKGAKEIILDDDECPLQIFREWPSDKGILVFQLKRRPPDHIPKKTKKHLE
  GKTPKGKERADGSGYGSTLPPEKLPYLVELSPGRRNHFAYYNYHTYEDGSDSRDKPKLYR
  LQLSVTEVGTEKLDDNSIQLFGPGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISET
  TMLQSGMKVQFGASHVFKFVDPSQDHALAKRSVDGGLMVKGPRHKPGIVQETTFDLGGDI
  HSGTALPTSKSTTRLDSDRVSSASSTAERGMVKPMIRVEQQPDYRRQESRTQDASGPELI
  LPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSNQYRPDISPTERT
  HKVIAVVNKMVSMMEGVIQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLA
  HLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMSLLRRCRVNA
  ALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHYWGAIIRQQLGHIEAWAEKQGLELAAD
  CHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIE
  NVVTVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLC
  QRGFCRLIPHTRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGL
  SIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTR
  TSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSTQNGS
  PESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSAYASGTTAKITSV
  STGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPPQNQWPNYEEKPHMHT
  DSNHSSIAIQRVTRSQEELREDKAYQLERHRIEAAMDRKSDSDMWINQSSSLDSSTSSQE
  HLNHSSKSVTPASTLTKSGPGRWKTPAAIPATPVAVSQPIRTDLPPPPPPPPVHYAGDFD
  GMSMDLPLPPPPSANQIGLPSAQVAAAERRKREEHQRWYEKEKARLEEERERKRREQERK
  LGQMRTQSLNPAPFSPLTAQQMKPEKPSTLQRPQETVIRELQPQQQPRTIERRDLQYITV
  SKEELSSGDSLSPDPWKRDAKEKLEKQQQMHIVDMLSKEIQELQSKPDRSAEESDRLRKL
  MLEWQFQKRLQESKQKDEDDEEEEDDDVDTMLIMQRLEAERRARLQDEERRRQQQLEEMR
  KREAEDRARQEEERRRQEEERTKRDAEEKRRQEEGYYSRLEAERRRQHDEAARRLLEPEA
  PGLCRPPLPRDYEPPSPSPAPGAPPPPPQRNASYLKTQVLSPDSLFTAKFVAYNEEEEEE
  DCSLAGPNSYPGSTGAAVGAHDACRDAKEKRSKSQDADSPGSSGAPENLTFKERQRLFSQ
  GQDVSNKVKASRKLTELENELNTK

• Sequence length: 1824