ADH5 (alcohol dehydrogenase 5 (class III), chi polypeptide)

Gene

• Entrez ID: 128
• Gene name: Alcohol dehydrogenase 5 (class III), chi polypeptide
• Gene symbol: ADH5
• Synonyms: ADH-3, ADHX, AMEDS, BMFS7, FALDH, FDH, GSH-FDH, GSNOR, HEL-S-60p
• Chromosome: 4
• Chromosome location: 4q23
• Summary: This gene encodes a member of the alcohol dehydrogenase family. Members of this family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. The encoded protein forms a homodimer. It has virtually no activity for ethanol oxidation, but exhibits high activity for oxidation of long-chain primary alcohols and for oxidation of S-hydroxymethyl-glutathione, a spontaneous adduct between formaldehyde and glutathione. This enzyme is an important component of cellular metabolism for the elimination of formaldehyde, a potent irritant and sensitizing agent that causes lacrymation, rhinitis, pharyngitis, and contact dermatitis. The human genome contains several non-transcribed pseudogenes related to this gene. [provided by RefSeq, Oct 2008]

miRNA

miRTarBase ID	miRNA	Experiments	Reference
MIRT020534	hsa-miR-155-5p	Proteomics	18668040
MIRT044260	hsa-miR-106b-5p	CLASH	23622248
MIRT441262	hsa-miR-218-5p	HITS-CLIP	23212916
MIRT447186	hsa-miR-4709-3p	PAR-CLIP	22100165
MIRT447186	hsa-miR-4709-3p	PAR-CLIP	22291592
MIRT447187	hsa-miR-4722-5p	PAR-CLIP	22100165
MIRT447187	hsa-miR-4722-5p	PAR-CLIP	22291592
MIRT447188	hsa-miR-1286	PAR-CLIP	22100165
MIRT447188	hsa-miR-1286	PAR-CLIP	22291592
MIRT447189	hsa-miR-4635	PAR-CLIP	22100165
MIRT447189	hsa-miR-4635	PAR-CLIP	22291592
MIRT447190	hsa-miR-873-5p	PAR-CLIP	22100165
MIRT447190	hsa-miR-873-5p	PAR-CLIP	22291592
MIRT447191	hsa-miR-5197-5p	PAR-CLIP	22100165
MIRT447191	hsa-miR-5197-5p	PAR-CLIP	22291592
MIRT450281	hsa-miR-6510-3p	PAR-CLIP	22100165
MIRT450282	hsa-miR-4668-5p	PAR-CLIP	22100165
MIRT450283	hsa-miR-549a	PAR-CLIP	22100165
MIRT450284	hsa-miR-372-5p	PAR-CLIP	22100165
MIRT450285	hsa-miR-371a-5p	PAR-CLIP	22100165
MIRT450286	hsa-miR-616-5p	PAR-CLIP	22100165
MIRT450287	hsa-miR-373-5p	PAR-CLIP	22100165
MIRT450288	hsa-miR-371b-5p	PAR-CLIP	22100165
MIRT450289	hsa-miR-3613-3p	PAR-CLIP	22100165
MIRT450290	hsa-miR-8087	PAR-CLIP	22100165
MIRT450291	hsa-miR-7161-5p	PAR-CLIP	22100165
MIRT450292	hsa-miR-548u	PAR-CLIP	22100165
MIRT540628	hsa-miR-4715-3p	PAR-CLIP	21572407
MIRT540629	hsa-miR-5589-3p	PAR-CLIP	21572407
MIRT540630	hsa-miR-6715b-5p	PAR-CLIP	21572407
MIRT540631	hsa-miR-6872-3p	PAR-CLIP	21572407
MIRT540632	hsa-miR-4269	PAR-CLIP	21572407
MIRT540633	hsa-miR-5582-5p	PAR-CLIP	21572407
MIRT620609	hsa-miR-4777-5p	HITS-CLIP	23824327
MIRT620610	hsa-miR-526a	HITS-CLIP	23824327
MIRT620611	hsa-miR-523-5p	HITS-CLIP	23824327
MIRT620612	hsa-miR-522-5p	HITS-CLIP	23824327
MIRT620613	hsa-miR-520c-5p	HITS-CLIP	23824327
MIRT620614	hsa-miR-519c-5p	HITS-CLIP	23824327
MIRT620615	hsa-miR-519b-5p	HITS-CLIP	23824327
MIRT620616	hsa-miR-519a-5p	HITS-CLIP	23824327
MIRT620617	hsa-miR-518f-5p	HITS-CLIP	23824327
MIRT620618	hsa-miR-518e-5p	HITS-CLIP	23824327
MIRT620619	hsa-miR-518d-5p	HITS-CLIP	23824327
MIRT620620	hsa-miR-6088	HITS-CLIP	23824327
MIRT620621	hsa-miR-4770	HITS-CLIP	23824327
MIRT620622	hsa-miR-143-3p	HITS-CLIP	23824327
MIRT620623	hsa-miR-520g-5p	HITS-CLIP	23824327
MIRT664422	hsa-miR-4282	HITS-CLIP	23824327
MIRT695398	hsa-miR-6516-5p	HITS-CLIP	23313552
MIRT695398	hsa-miR-6516-5p	PAR-CLIP	26701625
MIRT695398	hsa-miR-6516-5p	PAR-CLIP	27292025
MIRT695399	hsa-miR-455-3p	HITS-CLIP	23313552
MIRT695399	hsa-miR-455-3p	PAR-CLIP	26701625
MIRT695399	hsa-miR-455-3p	PAR-CLIP	27292025
MIRT695400	hsa-miR-1227-3p	HITS-CLIP	23313552
MIRT695400	hsa-miR-1227-3p	PAR-CLIP	26701625
MIRT695400	hsa-miR-1227-3p	PAR-CLIP	27292025
MIRT695401	hsa-miR-4451	HITS-CLIP	23313552
MIRT695401	hsa-miR-4451	PAR-CLIP	26701625
MIRT695401	hsa-miR-4451	PAR-CLIP	27292025
MIRT695402	hsa-miR-6799-5p	HITS-CLIP	23313552
MIRT695402	hsa-miR-6799-5p	PAR-CLIP	26701625
MIRT695402	hsa-miR-6799-5p	PAR-CLIP	27292025
MIRT695403	hsa-miR-4252	HITS-CLIP	23313552
MIRT695403	hsa-miR-4252	PAR-CLIP	26701625
MIRT695404	hsa-miR-6499-3p	HITS-CLIP	23313552
MIRT695404	hsa-miR-6499-3p	PAR-CLIP	26701625
MIRT695404	hsa-miR-6499-3p	PAR-CLIP	27292025
MIRT695405	hsa-miR-6086	HITS-CLIP	23313552
MIRT695405	hsa-miR-6086	PAR-CLIP	26701625
MIRT695405	hsa-miR-6086	PAR-CLIP	27292025
MIRT695406	hsa-miR-377-5p	HITS-CLIP	23313552
MIRT695406	hsa-miR-377-5p	PAR-CLIP	26701625
MIRT695406	hsa-miR-377-5p	PAR-CLIP	27292025
MIRT695407	hsa-miR-98-5p	HITS-CLIP	23313552
MIRT695407	hsa-miR-98-5p	PAR-CLIP	26701625
MIRT695407	hsa-miR-98-5p	PAR-CLIP	27292025
MIRT695408	hsa-miR-4500	HITS-CLIP	23313552
MIRT695408	hsa-miR-4500	PAR-CLIP	26701625
MIRT695408	hsa-miR-4500	PAR-CLIP	27292025
MIRT695409	hsa-miR-4458	HITS-CLIP	23313552
MIRT695409	hsa-miR-4458	PAR-CLIP	26701625
MIRT695409	hsa-miR-4458	PAR-CLIP	27292025
MIRT695410	hsa-let-7i-5p	HITS-CLIP	23313552
MIRT695410	hsa-let-7i-5p	PAR-CLIP	26701625
MIRT695410	hsa-let-7i-5p	PAR-CLIP	27292025
MIRT695411	hsa-let-7g-5p	HITS-CLIP	23313552
MIRT695411	hsa-let-7g-5p	PAR-CLIP	26701625
MIRT695411	hsa-let-7g-5p	PAR-CLIP	27292025
MIRT695412	hsa-let-7f-5p	HITS-CLIP	23313552
MIRT695412	hsa-let-7f-5p	PAR-CLIP	26701625
MIRT695412	hsa-let-7f-5p	PAR-CLIP	27292025
MIRT695413	hsa-let-7e-5p	HITS-CLIP	23313552
MIRT695413	hsa-let-7e-5p	PAR-CLIP	26701625
MIRT695413	hsa-let-7e-5p	PAR-CLIP	27292025
MIRT695414	hsa-let-7d-5p	HITS-CLIP	23313552
MIRT695414	hsa-let-7d-5p	PAR-CLIP	26701625
MIRT695414	hsa-let-7d-5p	PAR-CLIP	27292025
MIRT695415	hsa-let-7c-5p	HITS-CLIP	23313552
MIRT695415	hsa-let-7c-5p	PAR-CLIP	26701625
MIRT695415	hsa-let-7c-5p	PAR-CLIP	27292025
MIRT695416	hsa-let-7b-5p	HITS-CLIP	23313552
MIRT695416	hsa-let-7b-5p	PAR-CLIP	26701625
MIRT695416	hsa-let-7b-5p	PAR-CLIP	27292025
MIRT695417	hsa-let-7a-5p	HITS-CLIP	23313552
MIRT695417	hsa-let-7a-5p	PAR-CLIP	26701625
MIRT695417	hsa-let-7a-5p	PAR-CLIP	27292025
MIRT695418	hsa-miR-4487	HITS-CLIP	23313552
MIRT695418	hsa-miR-4487	PAR-CLIP	26701625
MIRT695418	hsa-miR-4487	PAR-CLIP	27292025
MIRT695419	hsa-miR-558	HITS-CLIP	23313552
MIRT695419	hsa-miR-558	PAR-CLIP	26701625
MIRT695419	hsa-miR-558	PAR-CLIP	27292025
MIRT695420	hsa-miR-3160-3p	HITS-CLIP	23313552
MIRT695420	hsa-miR-3160-3p	PAR-CLIP	26701625
MIRT695420	hsa-miR-3160-3p	PAR-CLIP	27292025
MIRT695421	hsa-miR-1247-3p	HITS-CLIP	23313552
MIRT695421	hsa-miR-1247-3p	PAR-CLIP	26701625
MIRT695421	hsa-miR-1247-3p	PAR-CLIP	27292025
MIRT695422	hsa-miR-6831-5p	HITS-CLIP	23313552
MIRT695422	hsa-miR-6831-5p	PAR-CLIP	26701625
MIRT695422	hsa-miR-6831-5p	PAR-CLIP	27292025
MIRT695423	hsa-miR-3927-3p	HITS-CLIP	23313552
MIRT695423	hsa-miR-3927-3p	PAR-CLIP	26701625
MIRT695423	hsa-miR-3927-3p	PAR-CLIP	27292025

Transcription factors

Transcription factor	Regulation	Reference
RARB	Unknown	1996113

Gene ontology (GO)

GO ID	Ontology	Definition	Evidence	Reference
GO:0001523	Process	Retinoid metabolic process	IEA
GO:0003016	Process	Respiratory system process	IEA
GO:0004024	Function	Alcohol dehydrogenase activity, zinc-dependent	IBA	21873635
GO:0005504	Function	Fatty acid binding	IDA	8460164
GO:0005739	Component	Mitochondrion	IEA
GO:0005829	Component	Cytosol	IBA	21873635
GO:0005829	Component	Cytosol	TAS
GO:0006069	Process	Ethanol oxidation	TAS
GO:0008270	Function	Zinc ion binding	IBA	21873635
GO:0008270	Function	Zinc ion binding	IDA	6375718
GO:0009055	Function	Electron transfer activity	TAS	8460164
GO:0010430	Process	Fatty acid omega-oxidation	IDA	16081420
GO:0018119	Process	Peptidyl-cysteine S-nitrosylation	IEA
GO:0018467	Function	Formaldehyde dehydrogenase activity	IDA	8460164
GO:0022900	Process	Electron transport chain	IEA
GO:0032496	Process	Response to lipopolysaccharide	IEA
GO:0042802	Function	Identical protein binding	IEA
GO:0045777	Process	Positive regulation of blood pressure	IEA
GO:0046294	Process	Formaldehyde catabolic process	IBA	21873635
GO:0051409	Process	Response to nitrosative stress	IEA
GO:0051775	Process	Response to redox state	IDA	8460164
GO:0051903	Function	S-(hydroxymethyl)glutathione dehydrogenase activity	IBA	21873635
GO:0051903	Function	S-(hydroxymethyl)glutathione dehydrogenase activity	TAS	8460164
GO:0070062	Component	Extracellular exosome	HDA	19056867, 23533145
GO:0106321	Function	S-(hydroxymethyl)glutathione dehydrogenase NADP activity	IEA
GO:0106322	Function	S-(hydroxymethyl)glutathione dehydrogenase NAD activity	IEA

Other IDs

• MIM: 103710
• HGNC: 253
• e!Ensembl: ENSG00000197894

Protein

• UniProt ID: P11766
• Protein name: Alcohol dehydrogenase class-3 (EC 1.1.1.1) (Alcohol dehydrogenase 5) (Alcohol dehydrogenase class chi chain) (Alcohol dehydrogenase class-III) (Glutathione-dependent formaldehyde dehydrogenase) (FALDH) (FDH) (GSH-FDH) (EC 1.1.1.-) (S-(hydroxymethyl)glutathione dehydrogenase) (EC 1.1.1.284)
• Protein function: Catalyzes the oxidation of long-chain primary alcohols and the oxidation of S-(hydroxymethyl) glutathione (PubMed:8460164). Also oxidizes long chain omega-hydroxy fatty acids, such as 20-HETE, producing both the intermediate aldehyde, 20-oxoarachidonate and the end product, a dicarboxylic acid, (5Z,8Z,11Z,14Z)-eicosatetraenedioate (PubMed:16081420). Class-III ADH is remarkably ineffective in oxidizing ethanol (PubMed:8460164).
• PDB: 1M6H, 1M6W, 1MA0, 1MC5, 1MP0, 1TEH, 2FZE, 2FZW, 3QJ5
• Family and domains:

  Pfam

  Accession	ID	Position in sequence	Description	Type
  PF08240	ADH_N	32 → 160	Alcohol dehydrogenase GroES-like domain	Domain
  PF00107	ADH_zinc_N	202 → 334	Zinc-binding dehydrogenase	Family

• Sequence:

  MANEVIKCKAAVAWEAGKPLSIEEIEVAPPKAHEVRIKIIATAVCHTDAYTLSGADPEGC
  FPVILGHEGAGIVESVGEGVTKLKAGDTVIPLYIPQCGECKFCLNPKTNLCQKIRVTQGK
  GLMPDGTSRFTCKGKTILHYMGTSTFSEYTVVADISVAKIDPLAPLDKVCLLGCGISTGY
  GAAVNTAKLEPGSVCAVFGLGGVGLAVIMGCKVAGASRIIGVDINKDKFARAKEFGATEC
  INPQDFSKPIQEVLIEMTDGGVDYSFECIGNVKVMRAALEACHKGWGVSVVVGVAASGEE
  IATRPFQLVTGRTWKGTAFGGWKSVESVPKLVSEYMSKKIKVDEFVTHNLSFDEINKAFE
  LMHSGKSIRTVVKI

• Sequence length: 374