ADH4 (alcohol dehydrogenase 4 (class II), pi polypeptide)

Gene

• Entrez ID: 127
• Gene name: Alcohol dehydrogenase 4 (class II), pi polypeptide
• Gene symbol: ADH4
• Synonyms: ADH-2, HEL-S-4
• Chromosome: 4
• Chromosome location: 4q23
• Summary: This gene encodes class II alcohol dehydrogenase 4 pi subunit, which is a member of the alcohol dehydrogenase family. Members of this enzyme family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. Class II alcohol dehydrogenase is a homodimer composed of 2 pi subunits. It exhibits a high activity for oxidation of long-chain aliphatic alcohols and aromatic alcohols and is less sensitive to pyrazole. This gene is localized to chromosome 4 in the cluster of alcohol dehydrogenase genes. [provided by RefSeq, Jul 2008]

miRNA

miRTarBase ID	miRNA	Experiments	Reference
MIRT549898	hsa-miR-4666a-5p	PAR-CLIP	21572407
MIRT549899	hsa-miR-8068	PAR-CLIP	21572407
MIRT549900	hsa-miR-143-5p	PAR-CLIP	21572407
MIRT549901	hsa-miR-3944-5p	PAR-CLIP	21572407
MIRT549902	hsa-miR-5087	PAR-CLIP	21572407
MIRT549903	hsa-miR-4764-5p	PAR-CLIP	21572407
MIRT549904	hsa-miR-657	PAR-CLIP	21572407
MIRT549905	hsa-miR-3665	PAR-CLIP	21572407
MIRT549906	hsa-miR-4722-5p	PAR-CLIP	21572407
MIRT549907	hsa-miR-1286	PAR-CLIP	21572407
MIRT549908	hsa-miR-873-5p	PAR-CLIP	21572407
MIRT549909	hsa-miR-4748	PAR-CLIP	21572407
MIRT549910	hsa-miR-4464	PAR-CLIP	21572407
MIRT549911	hsa-miR-6841-5p	PAR-CLIP	21572407
MIRT549912	hsa-miR-1265	PAR-CLIP	21572407
MIRT549913	hsa-miR-376c-3p	PAR-CLIP	21572407

Gene ontology (GO)

GO ID	Ontology	Definition	Evidence	Reference
GO:0001523	Process	Retinoid metabolic process	IDA	15369820
GO:0003960	Function	NADPH:quinone reductase activity	ISS
GO:0004022	Function	Alcohol dehydrogenase (NAD+) activity	IDA	3466164, 16787387
GO:0004024	Function	Alcohol dehydrogenase activity, zinc-dependent	IBA	21873635
GO:0004024	Function	Alcohol dehydrogenase activity, zinc-dependent	IDA	10407146
GO:0004032	Function	Alditol:NADP+ 1-oxidoreductase activity	IEA
GO:0004745	Function	Retinol dehydrogenase activity	IDA	10514444, 15369820, 16787387, 17279314
GO:0005503	Function	All-trans retinal binding	IDA	15369820
GO:0005515	Function	Protein binding	IPI	32296183
GO:0005654	Component	Nucleoplasm	IDA
GO:0005829	Component	Cytosol	IBA	21873635
GO:0005829	Component	Cytosol	IDA
GO:0005829	Component	Cytosol	TAS
GO:0006066	Process	Alcohol metabolic process	IDA	3466164
GO:0006069	Process	Ethanol oxidation	IDA	16787387
GO:0006069	Process	Ethanol oxidation	TAS
GO:0006081	Process	Cellular aldehyde metabolic process	IDA	3466164
GO:0008270	Function	Zinc ion binding	IBA	21873635
GO:0008270	Function	Zinc ion binding	IDA	10407146
GO:0010430	Process	Fatty acid omega-oxidation	IDA	16081420
GO:0016620	Function	Oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor	IDA	3466164
GO:0019115	Function	Benzaldehyde dehydrogenase [NAD(P)+] activity	IDA	3466164
GO:0019841	Function	Retinol binding	IDA	10407146
GO:0035276	Function	Ethanol binding	IEA
GO:0042572	Process	Retinol metabolic process	IDA	16787387, 17279314
GO:0046164	Process	Alcohol catabolic process	ISS
GO:0046294	Process	Formaldehyde catabolic process	IBA	21873635
GO:0051287	Function	NAD binding	IDA	10407146
GO:0051903	Function	S-(hydroxymethyl)glutathione dehydrogenase activity	IBA	21873635
GO:1901661	Process	Quinone metabolic process	ISS

Other IDs

• MIM: 103740
• HGNC: 252
• e!Ensembl: ENSG00000198099

Protein

• UniProt ID: P08319
• Protein name: All-trans-retinol dehydrogenase [NAD(+)] ADH4 (EC 1.1.1.105) (Alcohol dehydrogenase 4) (Alcohol dehydrogenase class II pi chain)
• Protein function: Catalyzes the NAD-dependent oxidation of either all-trans-retinol or 9-cis-retinol (PubMed:17279314). Also oxidizes long chain omega-hydroxy fatty acids, such as 20-HETE, producing both the intermediate aldehyde, 20-oxoarachidonate and the end product, a dicarboxylic acid, (5Z,8Z,11Z,14Z)-eicosatetraenedioate (PubMed:16081420). Also catalyzes the reduction of benzoquinones (PubMed:10514444).
• PDB: 3COS
• Family and domains:

  Pfam

  Accession	ID	Position in sequence	Description	Type
  PF08240	ADH_N	34 → 166	Alcohol dehydrogenase GroES-like domain	Domain
  PF00107	ADH_zinc_N	208 → 340	Zinc-binding dehydrogenase	Family

• Sequence:

  MGTKGKVIKCKAAIAWEAGKPLCIEEVEVAPPKAHEVRIQIIATSLCHTDATVIDSKFEG
  LAFPVIVGHEAAGIVESIGPGVTNVKPGDKVIPLYAPLCRKCKFCLSPLTNLCGKISNLK
  SPASDQQLMEDKTSRFTCKGKPVYHFFGTSTFSQYTVVSDINLAKIDDDANLERVCLLGC
  GFSTGYGAAINNAKVTPGSTCAVFGLGGVGLSAVMGCKAAGASRIIGIDINSEKFVKAKA
  LGATDCLNPRDLHKPIQEVIIELTKGGVDFALDCAGGSETMKAALDCTTAGWGSCTFIGV
  AAGSKGLTIFPEELIIGRTINGTFFGGWKSVDSIPKLVTDYKNKKFNLDALVTHTLPFDK
  ISEAFDLMNQGKSVRTILIF

• Sequence length: 380